Effect of Phosphorus on the Cadmium Transfer and ROS-scavenging Capacity of Rice Seedlings

Background: We observed that mitochondrial reactive oxygen species (mtROS) plays very important roles in the pregression of chagesic disease (CD). In this study, we utilized genetically-modified mice to scavenge mtROS to investigate the impact of improved ROS scavenging capacity on heart function in CD. Methods and Results: C57BL/6 mice (wild-type, MnSODtg, MnSOD+/-) were infected with Trypanosoma cruzi(Tc). Chronically infected mice (≥120dpi) exhibited a substantial decrease in heart tissue MnSOD gene expression, protein level, enzyme activity and antioxidant level; decrease of heart dysfunction via lower of SV, CO, EF, FS and LVPW,s, and increase of ESV/EDS and LVID;s; enhancement of hypertrophy by increase of IVS, LV mass and areas duo to augmentation of collagen expressions. One of our novel observations was that sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2) lost its role of maintenance of low cytoplasm free calcium and mediated calcium uptake to intracellular store in Tc-induced chronic chagasic disease. Studies of fresh heart slices using O2K confirmed that Tc diminished heart mitochondrial function like decrease of oxygen flux and respiratory control ratio (RCR), which were caused by enhancements of ROS. Myocardial mitochondrial damage was pronounced and associated with a >x% decline in mitochondrial oxygen flux in chronically infected wild-type and MnSOD transgenic mice. Imaging of intact heart for cardiomyocytes and collagen by the nonlinear optical microscopy techniques showed significant increase in collagen (>x0-fold) in chronically infected wild-type mice; while MnSODtg mice exhibited a basal increase in collagen that did not change during chronic phase. Chronically infected MnSODtg mice exhibited a marginal decline in Tc-induced heart function, heart hypertrophy, mitochondrial dysfunction Conclusions: Overexpression of MnSOD inhibited Tc-induced oxidative damage od heart tissue. , suggesting that enhancing the mitochondrial ROS scavenging capacity was beneficial in controlling the inflammatory and oxidative pathology, and cardiac remodeling responses that are hallmarks of chronic Chagas disease.

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Abstract B024: PKM2 activation suppresses cellular ROS scavenging capacity and potentiates doxorubicin antitumor activity

10.1158/1535-7163.targ-17-b024 ◽

2018 ◽

Author(s):

Peter Peterson ◽

Clifford J. Whatcott ◽

David J. Bearss ◽

Steven L. Warner ◽

Adam Siddiqui-Jain

Keyword(s):

Antitumor Activity ◽

Ros Scavenging ◽

Scavenging Capacity

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Facile Synthesis of Water-Soluble Fullerene (C60) Nanoparticles via Mussel-Inspired Chemistry as Efficient Antioxidants

Nanomaterials ◽

10.3390/nano9121647 ◽

2019 ◽

Vol 9 (12) ◽

pp. 1647 ◽

Cited By ~ 4

Author(s):

Xiaoyan Zhang ◽

Yihan Ma ◽

Sheng Fu ◽

Aiqing Zhang

Keyword(s):

Rational Design ◽

Water Soluble ◽

Photoelectron Spectra ◽

Ros Scavenging ◽

Laser Scattering ◽

Green Approach ◽

Transmission Electron ◽

Scavenging Capacity ◽

The Michael Addition

Rational design and modification of the all-carbon fullerene cages to meliorate their nature of hydrophobicity is critical for biomedical applications. The outstanding electron affinity of fullerenes enables them to effectively eliminate reactive oxygen species (ROS), the excess of which may lead to health hazards or biological dysfunction. Herein reported is a facile, mild, and green approach to synthesizing the favorable water-soluble C60 nanoparticles capable of ROS-scavenging by combining the mussel-inspired chemistry with the Michael addition reaction. Various characterization techniques, including Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectra (XPS), thermogravimetric analysis (TGA), transmission electron cryomicroscopy (Cryo-TEM), and dynamic laser scattering (DLS) were carried out to confirm the satisfactory preparation of the hybrid C60-PDA-GSH nanoparticles, which exhibited apparent scavenging capacity of DPPH and hydroxyl radicals in vitro. Additionally, the biocompatible C60-PDA-GSH nanoparticles entered into cells and displayed a universal cytoprotective effect against oxidative press induced by H2O2 in four kinds of human cells at a low concentration of 2 μg/mL. The ease and versatility of the strategy present in this work will not only trigger more fullerene-based materials by the immobilization of diverse functional molecules, but will also extend their possible applications.

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Endophytic infection modulates ROS-scavenging systems and modifies cadmium distribution in rice seedlings exposed to cadmium stress

Theoretical and Experimental Plant Physiology ◽

10.1007/s40626-019-00159-5 ◽

2019 ◽

Vol 31 (4) ◽

pp. 463-474 ◽

Cited By ~ 1

Author(s):

Lianju Ma ◽

Xuemei Li ◽

Lanlan Wang ◽

Yueying Li ◽

Ning Bu ◽

...

Keyword(s):

Cadmium Stress ◽

Rice Seedlings ◽

Ros Scavenging

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Effects of Bisphenol A on Oxidative Stress in the Rat Brain

Antioxidants ◽

10.3390/antiox9030240 ◽

2020 ◽

Vol 9 (3) ◽

pp. 240 ◽

Cited By ~ 2

Author(s):

Keiko Kobayashi ◽

Yanchen Liu ◽

Hiroshi Ichikawa ◽

Shigekazu Takemura ◽

Yukiko Minamiyama

Keyword(s):

Oxidative Stress ◽

Bisphenol A ◽

Ros Scavenging ◽

Adult Rat ◽

Systemic Oxidative Stress ◽

Scavenging Capacity ◽

Phosphorylated Akt ◽

Phosphatase 2A ◽

Related Proteins ◽

The Brain

We investigated the effect of bisphenol A (BPA) on oxidative stress and tau-related proteins in adult rat brains. BPA (10 mg/L) was administered to rats for eight weeks through their drinking water. The reactive oxygen species (ROS) scavenging capacity for hydroxyl radicals in the plasma was reduced after two weeks. In the hippocampus, four and eight weeks of BPA increased the ratio of oxidized DJ-1/DJ-1 (PARK7). The ratio of phosphorylated-GSK3β/GSK3β and phosphorylated-AKT/AKT increased after one week of BPA treatment. The ratio of phosphorylated JNK/JNK and phosphorylated-ERK/ERK increased after eight weeks of BPA; the elevation could be related to tau phosphorylation. Protein phosphatase 2A (PP2A) in the hippocampus decreased after eight weeks of BPA treatment. At that time, SOD1 was significantly induced, but no changes in SOD2 expression were apparent in the hippocampus. Furthermore, the ratio of phosphorylated-tau (PHF-1, Ser396/ Ser404) to total tau level did not change. However, PHF-1 or other sites of tau could be phosphorylated after eight weeks in the hippocampi of rats. BPA induced systemic oxidative stress and could change ROS-induced signaling pathways in the brain. These results suggest that mitochondrial dysfunction possibly is not responsible for oxidative stress and neurodegeneration due to low doses of BPA.

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The role of ascorbic acid in rice leaf senescence and photo–carbon imbalance

Functional Plant Biology ◽

10.1071/fp19248 ◽

2020 ◽

Vol 47 (3) ◽

pp. 263 ◽

Cited By ~ 1

Author(s):

Le Yu ◽

Qilei Zhang ◽

Lina Lu ◽

Hui Gao ◽

Qiang Liu ◽

...

Keyword(s):

Ascorbic Acid ◽

Leaf Senescence ◽

Photosynthetic Capacity ◽

Carbon Assimilation ◽

Main Idea ◽

Light Energy ◽

Ros Scavenging ◽

Rice Leaf ◽

Scavenging Capacity

Leaf senescence is an important factor that affects crop yield traits and is regulated by various factors. Here, we propose the photo–carbon imbalance hypothesis to explain the mechanism of rice leaf senescence. The main idea of this hypothesis is that carbon assimilation decreases faster than the absorption of light energy in photosynthesis during the late stages of rice growth, which ultimately results in leaf senescence. Our results indicate that endogenous ascorbic acid (Asc) plays an important role in leaf senescence by affecting the expression of senescence genes, thereby influencing photosynthetic capacity and consequently grain yield. The effects of exogenous Asc and methyl jasmonate (MeJA) on photosynthetic capability implied that the balance between photoreaction and carbon assimilation is regulated by exogenous antioxidants or accelerators of senescence. The results of the shading treatments indicated that shading will mitigate the photo–carbon imbalance and improve photosynthetic capacity, resulting in increased yields. Increasing antioxidant concentrations can enhance the reactive oxygen species (ROS) scavenging capacity, whereas shading reduces excess light energy, which may help to restore the photo–carbon balance.

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Oxygen radical scavenging capacity of phenolic and non-phenolic compounds in red and white wines

Open Life Sciences ◽

10.2478/s11535-011-0095-8 ◽

2012 ◽

Vol 7 (1) ◽

pp. 146-158 ◽

Cited By ~ 2

Author(s):

Roberto Scalzo ◽

Massimo Morassut ◽

Paolo Rapisarda

Keyword(s):

Hydroxyl Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Volatile Fraction ◽

Vitis Vinifera L ◽

Scavenging Activity ◽

Ros Scavenging ◽

White Wines ◽

Hydroxyl Radical Scavenging Activity ◽

Scavenging Capacity

AbstractThe aim of the present study was the evaluation of the antioxidant content in phenolic and non-phenolic extracts of ten wine samples, trying to elucidate the potential role of unusual antioxidant compounds. Samples of wines processed from red and white grapes (Vitis vinifera L.), deprived of the volatile fraction at low temperature and buffered at physiological pH, were fractionated by C18 into two fractions: FR1 and FR2. Non-phenolics, such as tartaric, malic, lactic, and succinic acids; glucose; fructose; and glycerin were mainly found in FR1, while polyphenols were present exclusively in FR2. Peroxyl radical quenching was assayed by the ORAC method, while superoxide and hydroxyl radical scavenging activity were assayed by electron paramagnetic resonance. In the ORAC and superoxide assays, most of the activity was found in FR2, while in hydroxyl radical assay, the activity was found in FR1. Model solutions were used to attribute a role to the single compounds in the evaluation of wine’s ROS scavenging capacity: the ORAC and superoxide anion scavenging effects were mainly attributed to the polyphenols, averaging 94.8%, with some contribution from glycerin, particularly in white wines. Unexpectedly, the main chemical responsible for hydroxyl radical scavenging activity was glycerin (56.1%), with the polyphenols scavenging at 18.1%.

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OsLEA1a overexpression enhances tolerance to diverse abiotic stresses by inhibiting cell membrane damage and enhancing ROS scavenging capacity in transgenic rice

Functional Plant Biology ◽

10.1071/fp20231 ◽

2021 ◽

Author(s):

Zhaodan Wang ◽

Qian Zhang ◽

Juan Qin ◽

Guosheng Xiao ◽

Shanshan Zhu ◽

...

Keyword(s):

Cell Membrane ◽

Transgenic Rice ◽

Abiotic Stresses ◽

Membrane Damage ◽

Ros Scavenging ◽

Cell Membrane Damage ◽

Scavenging Capacity

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Antioxidant Capacity of “Mexican Arnica”Heterotheca inuloidesCass Natural Products and Some Derivatives: Their Anti-Inflammatory Evaluation and Effect onC. elegansLife Span

Oxidative Medicine and Cellular Longevity ◽

10.1155/2015/843237 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 11

Author(s):

José Luis Rodríguez-Chávez ◽

Elvia Coballase-Urrutia ◽

Antonio Nieto-Camacho ◽

Guillermo Delgado-Lamas

Keyword(s):

Life Span ◽

Antioxidant Capacity ◽

Harmful Effect ◽

Inflammatory Activity ◽

Biological Processes ◽

Ros Scavenging ◽

C Elegans ◽

Scavenging Capacity ◽

Anti Inflammatory ◽

Stable Free Radicals

It has been suggested that the accumulation of biomolecular damage caused by reactive oxygen species (ROS) contributes to aging. The antioxidant activity is related to the ability of certain compounds to protect against the potentially harmful effect of processes or reactions involving ROS. This ability is associated with the termination of free radical propagation in biological systems. FromHeterotheca inuloidesvarious compounds which have shown to possess antioxidant capacity and scavenging ROS. The aim of this study was to determine the antioxidant capacity of additional natural components isolated fromH. inuloidesand some semisynthetic derivatives, their anti-inflammatory activity and the effect onCaenorhabditis elegansnematode life span. Compounds showed ability to inhibit various biological processes such as lipid peroxidation, scavenge nonbiological important oxidants such as1O2,OH∙, H2O2, and HOCl and scavenge non biological stable free radicals (DPPH). Some cadinane type compounds showed possess antioxidant, ROS scavenging capacity, anti-inflammatory activity, and effect on theC. eleganslife span. Flavonoid type compounds increased the life of the nematode and quercetin was identified as the compound with the greatest activity. The modification of chemical structure led to a change in the antioxidant capacity, the anti-inflammatory activity, and the survival of the worm.

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