CYTOTOXIC ACTIVITY OF AVOCADO SEEDS EXTRACTS (PERSEA AMERICANA MILL.) ON T47D CELL LINES

Research into plants with anticancer effects is actively encouraged in orderto discover new drugs with lessertoxicity but more potent effects. The aims of study are to evaluate the antioxidant properties and to investigate the cytotoxic activity of Plectranthus amboinicus (Lour.) Spreng. leaves ethyl acetate fractions on HeLa,T47D and MCF7 cell lines. The extract was prepared by graded maceration using n-hexane and ethyl acetate. The ethyl acetate extract was fractionated in vacuum liquid chromatography with n-hexane: ethyl acetate; and ethyl acetate: methanol as mobile phase. Then, the fractions were analyzed with thin layer chromatography (TLC). The free radical scavenging activity was measured by DPPH method, the total flavonoid content was calculated by quercetin equivalent and the absorbance is measured by using UV-Visible spectrophotometry. The cytotoxic activity were determined using MTT assay. The fractions contained 5 sub fractions with same TLC profile. The fractions showed antioxidant activity by DPPH method with different IC50 values, namely: 130 µg/mL(I), 127 µg/mL(II), 137 µg/mL(III), 129 µg/mL(IV), and 124 µg/ mL(V), respectively. The measurement of total flavonoid content showed 118 mg QE/g (I), 50 mg QE/g (II), 207 mg QE/g (III), 56 mg QE/g (IV), and 55 mg QE/g (V). The IC50 of each sub fractions on HeLa cell were 77 µg/mL, 46 µg/mL, 93 µg/mL, 71 µg/mL and 476 µg/mL; for T47D cell were 1621 µg/mL, 111 µg/mL, 128 µg/mL, 150 µg/mL and 209 µg/mL; and for MCF7 were 259 µg/mL, 343 µg/mL, 575 µg/mL, 408 µg/mL and 250 µg/mL. Based on the results, the fractions derived from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. leaves exhibit antioxidant. The Fraction II from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. was the most cytotoxic on HeLa, T47D and MCF7 cell lines. It is potential to undergo further isolation of its cytotoxic compounds.Keywords : antioxidant, cytotoxic, Plectranthus amboinicul (Lour.) Spreng., ethyl acetate fractions

Download Full-text

Selectivity Index of Alpinia galanga Extract and 1’-Acetoxychavicol Acetate on Cancer Cell Lines

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev10iss2pp95-100 ◽

2019 ◽

Vol 10 (2) ◽

pp. 95

Author(s):

Muhammad Da'i ◽

Khairunnisa Azani Meilinasary ◽

Andi Suhendi ◽

Sari Haryanti

Keyword(s):

Cytotoxic Activity ◽

Cancer Cells ◽

Cell Lines ◽

Cancer Cell ◽

Ethanol Extract ◽

T47d Cell ◽

Cancer Cell Lines ◽

Selectivity Index ◽

Methanol Fraction ◽

Alpinia Galanga

Previous research stated that galangal (Alpinia galanga) extract has a potential as cytotoxic agent with active compound of 1’-Acetoxychavicol Acetate (ACA). The objective of this study was to determine the selectivity of ethanol extract, ethyl acetate fraction, and methanol fraction of of galangal, and ACA on cancer cell lines. Cytotoxic activity was carried out using the MTT method on T47D breast cancer, WiDr colon cancer, HeLa cervical cancer, and Vero normal cell lines. The results showed that galangal ethanol extract and its fractions had selectivity index equal to or less than 2 on cancer cells. Meanwhile, ACA had selectivity index more than 3 on T47D cell and HeLa cell. ACA showed a strong cytotoxic activity against cancer cells T47D, HeLa, and WiDr with IC50 values of 3.14, 7.26, and 12.49 μg/ml, respectively. Based on data, it could be concluded that ACA was the most selective to inhibit T47D cell with a selectivity index of 6.6.Keywords: 1’-Acetoxychavicol acetate, galangal (Alpinia galanga), selective index, cytotoxic

Download Full-text

Acetoxy Chavicol Acetate (ACA) Concentration and Cytotoxic Activity of Alpinia galanga Extract on HeLa, MCF7 and T47D Cancer Cell Lines

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev8iss2pp81-84 ◽

2017 ◽

Vol 8 (2) ◽

pp. 81

Author(s):

Andi Suhendi ◽

Erindyah Retno Wikantyasning ◽

Gunawan Setyadi ◽

Arifah Sri Wahyuni ◽

Muhammad Da'i

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

T47d Cell ◽

Severe Side Effect ◽

Cytotoxic Activities ◽

Local Markets ◽

Alpinia Galanga ◽

Liquid Liquid Extraction ◽

Ic50 Value ◽

Ic50 Values

Due to severe side effect and non-specific chemotherapeutic agent, screening and discovery for cancer therapy are still working, especially from natural resources. Traditionally, people used herbal medicine either to prevent or cure diseases. One of herbal that commonly used in Indonesia is Alpinia galangal. Previous study stated that active compound is acetoxy chavicol acetate (ACA) and active as anticancer. This research aimed to determine ACA concentration and cytotoxic activity of Alpinia galanga extract (AGE) from three local markets on HeLa, MCF7 and T47D cell lines. The galangal used from three local markets namely Pasar Legi Surakarta, Beringharjo Yogyakarta, and Wonogiri. The extraction was performed by maceration using 96% ethanol as solvent. ACA quantitation using UV spectrophotometer at λ = 208.5 nm. Samples were prepared by liquid-liquid extraction using an ethyl acetate. Cytotoxic activities were performed by MTT assay. The result showed that the concentration of ACA of AGE from the three local markets were 3.798; 0.035; and 0.009 % w/w, respectively. Cytotoxic activity, describes as IC50 value, on HeLa cell line of AGE from three local markets, in order were 13.26; 36.32 and > 100 µg/ mL. Meanwhile, AGE from Pasar Legi on MCF7 and T47D cell lines have IC50 value of 15.80; 12.50 µg/ mL, respectively. In contrast, two other samples have IC50 values of greater than 100 µg/ mL. The highest activity was from the highest concentration of ACA on the samples.Key words: Alpinia galanga, HeLa, T47D, MCF7 and Acetoxy chavicol acetate

Download Full-text

Cytotoxic Activity and Secondary Metabolite Characteristics of Sea Cucumber Actinopyga sp. Methanolic Extract

SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology ◽

10.15578/squalen.v11i1.193 ◽

2016 ◽

Vol 11 (1) ◽

pp. 23

Author(s):

Muhammad Nursid ◽

Afrilia Putri Maharani ◽

Riyanti Riyanti ◽

Endar Marraskuranto

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Sea Cucumber ◽

Ion Trap ◽

Methanolic Extract ◽

T47d Cell ◽

Breast Cancer Cell Lines ◽

Mtt Method ◽

T47d Breast Cancer Cell ◽

Tof Ms

The objectives of this research were to assess cytotoxic activity and examine characteristic of sea cucumber Actinopyga sp. methanolic extract. Cytotoxicity assay was conducted using MTT method against WiDr (colon cancer) and T47D (breast cancer) cell lines. Actinopyga sp. methanolic extract was characterized by using phytochemical screening, Transform-Infrared (FT-IR) spectroscopy, dan Liquid Chromatography Ion Trap Time of Flight Mass Spectrophotometer (LC-IT-ToF-MS). The result showed that Actinopyga sp. methanolic extract inhibited Widr and T47D cell lines viability with the IC50 value of 55.93 and 87.55 µg/mL, respectively. Functional groups analysis showed the presence of hydroxyl, amine, carboxylic acid, nitrate, amide, sulphur, ester, and ether. LC-IT-ToF-MS analysis of crude extract showed that it containing many steroids compound.

Download Full-text

CYTOTOXIC ACTIVITY AND APOPTOSIS INDUCTION OF T47D CELL LINES BY Turbinaria decurrens EXTRACT

SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology ◽

10.15578/squalen.v8i1.78 ◽

2013 ◽

Vol 8 (1) ◽

pp. 23 ◽

Cited By ~ 1

Author(s):

Muhammad Nursid ◽

Nurrahmi Dewi Fajarningsih ◽

Ekowati Chasanah

Keyword(s):

Flow Cytometry ◽

Ethyl Acetate ◽

Cytotoxic Activity ◽

Cell Lines ◽

Crude Extract ◽

T47d Cell ◽

Apoptosis Induction ◽

Flow Cytometry Analysis ◽

Methanol Fraction ◽

Ic50 Value

Marine algae is known to contain a wide variety of biomedical compounds having pharmaceutical applications. The aim of this research was to evaluate cytotoxic activity and apoptosis induction of Turbinaria decurrens extract on T47D cell lines. Cytotoxic activity test was conducted by using MTT assay whereas detection of apoptosis was evaluated by DNA fragmentations and flow cytometry analysis. The MTT test showed that crude extract had medium cytotoxic activity to T47D, HepG2, and C28 cell lines with IC50 value of 172, againts 360 and 330 µg ml-1, respectively. After solvent partition of crude extract, the cytotoxic activity of n-hexane and ethyl acetate fractions T47D cell increased, the cytotoxic activity of n. hexane and ethyl acetate fractions T47D cell increased with IC50 value of with IC50 43.1 and 51.9 µg ml-1, respectively, whereas IC50 value of methanol fraction was 383.0 µg ml-1. Analysis of DNA fragmentation of T47D cell showed that both n-hexane and ethyl acetate fractions could not fragment DNA as a features of apoptosis. However, flow cytometry analysis by using annexin-V and propidium iodide staining revealed that n-hexane and ethyl acetate fractions could induce apoptosis in T47D cell. This research indicated that Turbinaria decurrens had potency to induce apoptosis in T47D cells.

Download Full-text

Sitotoksisitas in vitro ekstrak etanolik buah parijoto (Medinilla speciosa, reinw.ex bl.) terhadap sel kanker payudara T47D

JURNAL GIZI INDONESIA ◽

10.14710/jgi.2.2.53-58 ◽

2014 ◽

Vol 2 (2) ◽

pp. 53-58 ◽

Cited By ~ 1

Author(s):

Iin Tusanti ◽

Andrew Johan ◽

RA Kisdjamiatun

Keyword(s):

Breast Cancer ◽

Cytotoxic Activity ◽

Cell Line ◽

Cell Lines ◽

Colorimetric Assay ◽

Ethanolic Extract ◽

T47d Cell ◽

Ic50 Value ◽

T47d Cell Line

Background: Several studies focused on phytochemical as agents of cancer prevention and co-chemotherapy. One of Indonesian plant which has edible fruit but it hasn’t been completely explored is Medinilla speciosa (Reinw.ex Bl.). Objective : The aim of this study is to examine the cytotoxic activity (IC50 value) of Medinilla speciosa (Reinw.ex Bl.) fruit ethanolic extract. Methods : Medinilla speciosa (Reinw.ex Bl.) fruit ethanolic extract was used in this study. The cytotoxic activity was investigated in vitro on human breast cancer T47D cell-line. The cells viability were assessed using MTT colorimetric assay. Breast cancer T47D cell lines was treated with fruit ethanolic extract (10, 25, 50, 100, 250, 500 and 1000 µg/ml) for 24 hour of incubation. This study also identified phytochemical compound of the fruit with thin layer chromatography (TLC). Results: The result showed that ethanolic extract of Medinilla speciosa (Reinw.ex Bl.) has moderate cytotoxicity on breast cancer T47D cell line with IC50 value of 614.50 µg/ml and yield the decrease of cell viability at higher concentration. Medinilla speciosa fruit can not be used as anticancer agent but chemoprevention agent. Phytochemical test showed that the fruit extract contain flavonoid and saponin compound. Conclusion: Ethanolic extract of Medinilla speciosa fruit exhibited moderate cytotocicity on breast cancer T47D cell lines with IC50 value was 614,50 µg/ml thus it can be used as chemopreventioan agent.

Download Full-text