scholarly journals Histological and Histochemical Study of Radiofrequency Radiation effects on the Hippocampus during the Pre- and Postnatal Stages of Development

2021 ◽  
pp. 51-64
Author(s):  
Joshua Oladele Owolabi ◽  
Olayinka Stephen Ilesanmi
Keyword(s):  
Radiation Effects ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Hippocampal Formation ◽  
Later Life ◽  
Primary Objective ◽  
Postnatal Exposure ◽  
Experimental Modelling ◽  
Radiofrequency Radiation ◽  
Functional Integrity

Background: The research was designed to model the exposure to radiofrequency radiation (RFR) by habitual users of RFR-enabled devices and to observe possible aberrations in tissues that are attributable to exposures. The RFR exposure regimen modelled cases of continuous, and intermittent exposures in human conditions, using Wistar rats. The primary objective of the study was to study intrauterine and postnatal exposure to RFR and study its effects on specific brain structural and functional attributes. Materials and Methods: Experimental Wistar rats were housed in facilities that enabled exposure to specific type of RFR source (the 4G RFR-emitting internet router) and for specific durations which included 21 days of pregnancy and 35 post-natal days, marking the point of puberty. Following exposure, animals were sacrificed to excise brain tissues for histological analysis using the haematoxylin and eosin technique, histochemical analysis using the Nissl technique, and immunohistochemical techniques including the IBA 1 and Caspase 3 techniques for inflammation and potential apoptosis. Representative histological and histochemical photomicrographs were analysed using principles of qualitative histology and histochemistry. Results and Conclusion: Findings from the current research showed that RFR-exposure did not produce teratogenic or neurodegenerative effects within the hippocampus. This was evident from the study of the hippocampus’ histoarchitectural organisation, morphologies of the cells as well as their spatial distribution.  Functional integrity of cells in the different regions of the hippocampal formation, namely the CA 1-4 areas as well as the dentate gyrus also showed that Nissl substance expression, which is a marker of neuron functional integrity, was relatively normally expressed across the experimental animals.  This experimental modelling of human habitual exposure to RFR showed no evidence of prenatal teratogenic effects or postnatally induced extensive neurodegeneration up until puberty. However, it would be very important to indicate that RFR-exposure enhanced apoptotic potentials via the Caspase-3 pathway. The implications of this effect on later life mental health and neurological attributes will require further investigation

scholarly journals A Study of RFR Effects on the Brain Neurochemistry during Pre- and Postnatal Brain Development

2021 ◽  
pp. 53-63
Author(s):  
Joshua Oladele Owolabi ◽  
Olayinka Stephen Ilesanmi ◽  
Vimla Luximon-Ramma
Keyword(s):  
Wistar Rats ◽  
Later Life ◽  
Brain Structures ◽  
Primary Objective ◽  
Postnatal Exposure ◽  
Pregnant Rats ◽  
Tissue Samples ◽  
Mean Values ◽  
Radiofrequency Radiation ◽  
Prolonged Duration

Background: The research was designed to model animals which mimic the exposure to the radiofrequency radiation [RFR] by habitual users of RFR-enabled devices and to observe possible aberrations in behaviours that are attributable to exposures. The research was designed to model cases of continuous, and intermittent exposures in human conditions, using Wistar rats. The primary objective of this study was to study intrauterine and postnatal exposure and study the effects brain structures, functions and behaviours in Wistar rats. Materials and Methods: The experiment started with 42 pregnant rats that were exposed to RFR [4G] to observe the possibilities of RFR-induced teratogenic effects. Thereafter, half of the offspring were sacrificed for their brains to be studied at birth. The other half were exposed to RFR to observe postnatal effects of RFR radiation until puberty. The exposure regimen was the same for the mothers and the offspring in each group. What varied was he duration of exposure per day being 6 hours, 24 hours and 24 hours. Experimental Wistar rats were housed in facilities that enable exposure to specific dose (4G) of radiations during pregnancy (~21 days) of and during post-natal days until day 35, marking approximately the point of puberty. Following the dissection of the rat, brain tissue samples were homogenized in buffered phosphate saline [PBS]. The homogenates were centrifuged, and the supernatant was assayed for each neurotransmitter of interest. The values from each sample were recorded for the sample. The mean values for samples that constituted a group was calculated and statistically analyzed. ELISA biochemical assay kits were used.     Results and Conclusion: RFR-exposure caused changes in neurotransmitters and enzyme neurochemistry.  Cytochrome C oxidase enzyme and neurotransmitters especially dopamine, gamma-amino butyric acid , glutamine and serotonin and their activities levels were significantly altered especially with prolonged duration in RFR exposure. These findings would altogether suggest that radiofrequency radiation exposure might change brain neurochemistry permanently following exposure either during the intrauterine or the postnatal stage of life. The implications of these changes on later life mental health and neurological attributes will require further investigation

scholarly journals A Study of 4G Radiofrequency Radiation effects on Juvenile Wistar Rats Cerebellum and Potential Attenuative Properties of Fish Oil Omega-3 Fat

2021 ◽  
pp. 6-19
Author(s):  
Fabiyi Oluwaseyi Sunday ◽  
Ogunbiyi Olubunmi ◽  
C. Odusote Ifeoluwa ◽  
Adelakin Lola ◽  
Olanrewaju John Afees ◽  
...  
Keyword(s):  
Fish Oil ◽  
Radiation Effects ◽  
Wistar Rats ◽  
Pubertal Development ◽  
Spatial Arrangement ◽  
Barnes Maze ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Radiofrequency Radiation ◽  
Living Organisms

Introduction: Technological devices have become a very important part of our everyday life. These electronic devices create an artificial electromagnetic field (EMF) and emit radiofrequency radiations (RFR) which have been reported to have deleterious effects on various tissues in living organisms. Fish oil is a rich source of the n-3 (also known as omega-3) fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The cerebellum plays an important role in motor control and the coordination, precision and timing of movements, as well as in motor learning. This study investigated the effect of radiofrequency radiation on the structures of the cerebellum of adolescent Wistar rats, its effect on pre-pubertal development and the potential attenuative properties of fish oil omega-3 fat. Materials and Methods: Forty (n=40) adolescent Wistar rats of approximately 35 days old were divided into five groups labelled A-E. A 4G RFR-emitting WI-FI device served as the RFR source. The 4G radiofrequency transmitter was installed at a distance of 0.5 meters from the base of the cage housing the experimental animals in each group. The fish oil was administered orally to the animals. Rats were sacrificed after 28 days of treatment and their brains harvested. The cerebellar tissues were excised and processed for histological, immunohistochemical and biochemical observations. Neurobehavioural studies including Barnes Maze and beam walk were carried out before sacrifice. Results: 4G radiofrequency radiation caused distortions in the morphology and spatial arrangement of Purkinje cells in the cerebellar tissues. It also induced neuroinflammation as marked by enhanced astrocyte reactions. Also, behavioural aberrations including negative changes in key memory parameters such as increased latency and error poke in Barnes maze assays, and increased movement parameter errors were attributable to FRR effects. Also, aberrations in neurotransmitters activities levels and vital neurochemistry enzymes were observed. The administered Omega-3 fat had an attenuative effect on the affected cortical structures, neurochemistry and selected neurobehavioural parameters.

P–061 Protective effect of melatonin against bleomycin, etoposide, and cisplatin (BEP) chemotherapy-induced testicular toxicity in Wistar rats: A biochemical, immunohistochemical and apoptotic genes based evidence

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
M Moradi ◽  
A Faramarzi ◽  
N Goodarzi ◽  
A H Hashemian ◽  
H Cheraghi ◽  
...  
Keyword(s):  
Testosterone Level ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Treated Group ◽  
Serum Testosterone Level ◽  
Dependent Manner ◽  
Qrt Pcr ◽  
Tnf Α ◽  
Significant Difference ◽  
Bep Chemotherapy

Abstract Study question Does exogenous melatonin (MLT) attenuate BEP-induced damage in testicular cells and spermatogenesis in a dose-dependent manner? Summary answer Melatonin protected the testes against BEP-induced testis damage through ameliorating nitro-oxidative stress, apoptosis, and inflammation. However, there was no significant difference between melatonin-treated groups. What is known already Recently, the prevalence of testicular cancer (TC), accounting for the most common cancer among young people of reproductive age (15–40 years), has risen internationally. BEP chemotherapy has increased the 5-year survival rate of TC patients at all stages of testicular germ cell tumors to 90–95%. However, BEP creates a high incidence of male infertility and even long-term genotoxic effects, which emerges as a critical health issue. Melatonin is a well-known potent antioxidant with widespread clinical applications that recently has been giving increasing attention to its role in male sub/infertility. Study design, size, duration 60 Adult male Wistar rats were randomly assigned to six groups (n = 10/group). Group 1, 3, and 4 were injected with vehicle, 10 and 20 mg/kg of melatonin, respectively. Other groups received one cycle of bleomycin, etoposide, and cisplatin for a total of 3 weeks with or without melatonin. Melatonin administration started daily one week before BEP initiation continued on days 2, 9, and 16; and one week after the completion of the BEP cycle. Participants/materials, setting, methods Bodyweight, testes weight, Sperm parameters (count, motility, viability, and morphology), testosterone hormone level, testicular histopathology, stereological parameters, testicular level of malondialdehyde (MDA), nitric oxide (NO), and total antioxidant capacity (TAC), the expression of Bcl–2, Bax, Caspase–3, p53, and TNF-α (Real-time PCR and immunohistochemistry) were evaluated at the end of the study (day 35). Main results and the role of chance Our findings showed that melatonin restores the BEP-induced reduction in the body and testes weight (P<.05). the evaluation of quantitative analysis of the testes stereological procedures, QRT-PCR examination and immunohistochemical (IHC) staining revealed that melatonin reverses the BEP-induced impaired spermatogenesis (P<.05). Furthermore, melatonin rectifies BEP-induced disturbance on sperm count, motility, viability, and morphology. The testosterone level in the BEP-treated group was decreased significantly by comparison with the control group (P<.01). By contrast, co-administration of 10 and 20 mg/kg of melatonin could enhance the serum testosterone level significantly (P<.05). Moreover, melatonin enhanced the antioxidant status of the testis by elevating TAC and ameliorating MDA and NO levels. More notably, QRT-PCR examination indicated that melatonin therapy suppressed BEP-induced apoptosis by modulating apoptosis-associated genes such as Bcl–2, Bax, Caspase–3, p53 in the testis (P<.01). Besides, Co-administration of 10 and 20 mg/kg of melatonin with BEP regimen decreased significantly the population of p53 (54.21 ±6.18% and 51.83±8.45, respectively) and TNF-α positive cells (42.91±9.92% and 33.57±2.97, respectively) by comparison to the BEP group. Also, melatonin with low and high doses could enhance the expression of Bcl–2 protein in spermatogenic cells line (59.19±10.18%, 63.08±5.23, respectively) compared to the BEP-treated group. Limitations, reasons for caution Owing to limited laboratory facilities we were not able to perform further studies to verify the mechanism of melatonin in the specific targets by using transfection technique and transgenic. Wider implications of the findings: These findings can draw attention to the clinical application of melatonin and also suggest that melatonin may be an attractive agent for attenuating chemotherapy-associated male sub/infertility. This indolamine also may shorten the fertility recovery period in patients undergoing chemotherapy with the BEP regimen. Trial registration number N/A

scholarly journals Effectiveness of Vitamin C and Vitamin E Antioxidant Combination on Caspase-3 Expression in Wistar White Rat Pulmonum Contusion

2020 ◽  
Vol 3 (1) ◽  
pp. 31-44
Author(s):  
Bermansyah ◽  
Gama Satria ◽  
Ahmad Umar
Keyword(s):  
Cell Death ◽  
Vitamin E ◽  
Vitamin C ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Cell Function ◽  
Pulmonary Contusion ◽  
Tnf Α ◽  
Male Wistar Rats

Introduction.Pulmonary contusions can cause a progressive inflammatory response. Activation of TNF-α cytokines and reactive oxygen species (ROS) can cause pulmonary cell death. Antioxidants can have the potential to neutralize ROS. The purpose of this study is to determine the effectiveness of antioxidant administration in maintaining pulmonary cell function in wistar rats that have been induced to experience pulmonary contusions through caspase-3 levels. Methods.This study was an in vivo experimental study conducted on thirty male wistar rats and divided into five groups (n = 6): control, pulmonary contusion + asthaxanthine 5 mg/kgBW, pulmonary contusion + vitamin C and E 50 mg/kgBW, pulmonary contusion + vitamin C and E 100 mg/kgBW, pulmonary contusion + vitamin C and E 200 mg/kgBW. The value of Caspase-3 is evaluated by the IHC. All data analyzes used SPSS 18. Results. Low doses of antioxidants have the potential to reduce pulmonary cell death in wistar rats induced by pulmonary contusions.Conclussion. Vitamin C and E effective to reduce polmonary cell death in pulmonary contusion.Keywords: antioxidants, vitamin C, vitamin E, pulmonary contusions animal model, apoptosis, caspase-3

scholarly journals Haloperidol and Risperidone Induce Apoptosis Neuronal Cell : Invivo Study

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Ester G Panserga ◽  
Cecep S Kristanto ◽  
Budi Pratiti ◽  
Patricia Wulandari
Keyword(s):  
Cell Apoptosis ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Neuronal Cells ◽  
Neuronal Cell ◽  
Total Percentage ◽  
White Rats ◽  
Male Wistar Rats ◽  
Group B

Abstract Introduction Antipsychotics are drugs that are widely prescribed for mental disorders, such as schizophrenia and psychosis. Recent in vitro studies show antipsychotics play a role in the initiation of neuronal cell apoptosis. This study aims to determine the effect of haloperidol and risperidone on neuronal cell apoptosis in Wistar white rats. Methods Male wistar rats aged 8 weeks (n = 30) were used in this study. Wistar rats were randomized into 6 groups. Group A: 5 wistar rats as a control without induced schizophrenia, aquades and drugs. Group B: 5 Wistar-induced psychotic mice (using 30 mg / kgBB ketamine, intraperitoneal injection for 5 days) and aquadest. Group C: 5 rats were induced psychotic and were given haloperidol or 0.05 mg / kgBB orally, for 28 days. Group D: 5 mice were induced psychotic and were given haloperidol 0.1 mg / kg orally, for 28 days. Group E: 5 mice were induced psychotic and were given risperidone 0.05 mg / kgBB orally, for 28 days. Group F: 5 mice were induced psychotic and given risperidone 0.1 mg / kgBB orally, for 28 days. Apoptosis of neuronal cells in the ventral tegmental area was assessed by caspase-3 immunohistochemistry. The colored area will be calculated as a total percentage using the imageJ program. Results Risperidone and haloperidol increase caspase-3 activity, but haloperidol increases caspase-3 activity more than risperidone. Conclussion Risperidone and haloperidol induce apoptosis of neuronal cells and tardive dyskinesia in Wistar rats with psychotic models.

Investigation of Radiofrequency Radiation Effects on Excitable Tissues

1980 ◽  
Author(s):  
Everette C. Burdette ◽  
Ronald L. Seaman ◽  
Robert L. DeHaan ◽  
R. C. Lantz
Keyword(s):  
Radiation Effects ◽  
Radiofrequency Radiation

scholarly journals Towards predicting intracellular radiofrequency radiation effects

PLoS ONE ◽  
2019 ◽  
Vol 14 (3) ◽  
pp. e0213286 ◽  
Author(s):  
Claus Nielsen ◽  
Ron Hui ◽  
Wing-Yee Lui ◽  
Ilia A. Solov’yov
Keyword(s):  
Radiation Effects ◽  
Radiofrequency Radiation

scholarly journals Postnatal exposure to cocaine in rats housed in an enriched environment: effects on social interactions

2007 ◽  
Vol 26 (4) ◽  
pp. 303-309 ◽  
Author(s):  
Ana Magalhães ◽  
Teresa Summavielle ◽  
Maria Amélia Tavares ◽  
Liliana de Sousa
Keyword(s):  
Social Interactions ◽  
Wistar Rats ◽  
Environmental Enrichment ◽  
Postnatal Life ◽  
Postnatal Exposure ◽  
Social Investigation ◽  
Social Challenges ◽  
Play Fighting ◽  
Environment Effects ◽  
Cocaine Hydrochloride

This study was undertaken to evaluate the effects of environmental enrichment (EE) in rats exposed to cocaine during the first month of postnatal life by examining several categories of social behaviour (play fighting, social investigation, comfort behaviours and invitation to play). Wistar rats were divided in four groups: pups exposed to cocaine hydrochloride (15 mg/kg body weight/day), sc, in two daily doses, from postnatal day (PND) 1 to 28 and reared in EE; exposed to cocaine as previously described and reared in standard environment (SE); saline-exposed and reared in EE; pups saline-exposed and reared in SE. On PND 21, 24 and 28, social interactions were examined for 10 min. Results show that cocaine animals reared in SE decreased the frequency of play solicitation. Control animals reared in EE exhibited decreased play fighting and social investigation behaviours compared to SE-reared rats. Animals postnatally exposed to cocaine when reared in EE displayed more comfort and invitation to play behaviours and decreased social investigation compared with SE-reared animals. The results suggest that in rats postnatally exposed to cocaine, EE rearing elicited differences in both processing of environmental stimuli and a response to social challenges. Human & Experimental Toxicology (2007) 26, 303-309

scholarly journals Association between early-life exposure to the Great Chinese Famine and poor physical function later in life: a cross-sectional study

BMJ Open ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. e027450
Author(s):  
Tao Tao ◽  
Lingyan Dai ◽  
JinXiang Ma ◽  
Yusi Li ◽  
Zhuoyuan Guo
Keyword(s):  
Physical Function ◽  
Early Life ◽  
Later Life ◽  
Exposed Group ◽  
Self Report ◽  
Postnatal Exposure ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Fetal Period ◽  
Early Life Exposure

ObjectivesThis study aimed to evaluate the association between early-life exposure to the Great Chinese Famine (1959–1961) and the prevalence of poor physical function in midlife.DesignA population-based historical prospective study was performed as part of a wider cross-sectional survey. Exposure to famine was defined by birthdate, and participants were divided into non-exposed group, fetal-exposed group and infant-exposed group.Setting and participantsA total of 3595 subjects were enrolled into the study from the China Health and Retirement Longitudinal Study (CHARLS) 2015 based on random selection of households that had at least one member aged 45 years old and older in 28 provinces of mainland China.Main outcome measuresPhysical function status was assessed by a six-item self-report on the Barthel scale which rated basic activities of daily living (BADL).Results743 (20.7%) out of all participants were exposed to the Great Chinese Famine in their fetal periods, while 1550 (43.1%) participants were exposed at the age of an infant. The prevalence of poor physical function in the non-exposed group, fetal period-exposed group and infant period-exposed group were 12.3%, 15.5% and 17.0%, respectively. Among males, after stratification by gender and severity of famine, the prevalence of poor physical function in the fetal period was significantly higher (OR 2.40, 95% CI 1.18 to 4.89, p=0.015) than the non-exposed group in severely affected areas, even after adjusting for the number of chronic diseases, place of residence, smoking and alcohol drinking habits, marital status, educational level and body mass index. A similar connection between prenatal and early postnatal exposure to the Great Chinese Famine and the prevalence of poor physical function in midlife, however, was not observed from female adults.ConclusionsMales who were exposed to the Great Chinese Famine (1959–1961) present considerably decreased physical function in their later life.

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