scholarly journals A Study of RFR Effects on the Brain Neurochemistry during Pre- and Postnatal Brain Development

2021 ◽  
pp. 53-63
Author(s):  
Joshua Oladele Owolabi ◽  
Olayinka Stephen Ilesanmi ◽  
Vimla Luximon-Ramma
Keyword(s):  
Wistar Rats ◽  
Later Life ◽  
Brain Structures ◽  
Primary Objective ◽  
Postnatal Exposure ◽  
Pregnant Rats ◽  
Tissue Samples ◽  
Mean Values ◽  
Radiofrequency Radiation ◽  
Prolonged Duration

Background: The research was designed to model animals which mimic the exposure to the radiofrequency radiation [RFR] by habitual users of RFR-enabled devices and to observe possible aberrations in behaviours that are attributable to exposures. The research was designed to model cases of continuous, and intermittent exposures in human conditions, using Wistar rats. The primary objective of this study was to study intrauterine and postnatal exposure and study the effects brain structures, functions and behaviours in Wistar rats. Materials and Methods: The experiment started with 42 pregnant rats that were exposed to RFR [4G] to observe the possibilities of RFR-induced teratogenic effects. Thereafter, half of the offspring were sacrificed for their brains to be studied at birth. The other half were exposed to RFR to observe postnatal effects of RFR radiation until puberty. The exposure regimen was the same for the mothers and the offspring in each group. What varied was he duration of exposure per day being 6 hours, 24 hours and 24 hours. Experimental Wistar rats were housed in facilities that enable exposure to specific dose (4G) of radiations during pregnancy (~21 days) of and during post-natal days until day 35, marking approximately the point of puberty. Following the dissection of the rat, brain tissue samples were homogenized in buffered phosphate saline [PBS]. The homogenates were centrifuged, and the supernatant was assayed for each neurotransmitter of interest. The values from each sample were recorded for the sample. The mean values for samples that constituted a group was calculated and statistically analyzed. ELISA biochemical assay kits were used.     Results and Conclusion: RFR-exposure caused changes in neurotransmitters and enzyme neurochemistry.  Cytochrome C oxidase enzyme and neurotransmitters especially dopamine, gamma-amino butyric acid , glutamine and serotonin and their activities levels were significantly altered especially with prolonged duration in RFR exposure. These findings would altogether suggest that radiofrequency radiation exposure might change brain neurochemistry permanently following exposure either during the intrauterine or the postnatal stage of life. The implications of these changes on later life mental health and neurological attributes will require further investigation

scholarly journals Histological and Histochemical Study of Radiofrequency Radiation effects on the Hippocampus during the Pre- and Postnatal Stages of Development

2021 ◽  
pp. 51-64
Author(s):  
Joshua Oladele Owolabi ◽  
Olayinka Stephen Ilesanmi
Keyword(s):  
Radiation Effects ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Hippocampal Formation ◽  
Later Life ◽  
Primary Objective ◽  
Postnatal Exposure ◽  
Experimental Modelling ◽  
Radiofrequency Radiation ◽  
Functional Integrity

Background: The research was designed to model the exposure to radiofrequency radiation (RFR) by habitual users of RFR-enabled devices and to observe possible aberrations in tissues that are attributable to exposures. The RFR exposure regimen modelled cases of continuous, and intermittent exposures in human conditions, using Wistar rats. The primary objective of the study was to study intrauterine and postnatal exposure to RFR and study its effects on specific brain structural and functional attributes. Materials and Methods: Experimental Wistar rats were housed in facilities that enabled exposure to specific type of RFR source (the 4G RFR-emitting internet router) and for specific durations which included 21 days of pregnancy and 35 post-natal days, marking the point of puberty. Following exposure, animals were sacrificed to excise brain tissues for histological analysis using the haematoxylin and eosin technique, histochemical analysis using the Nissl technique, and immunohistochemical techniques including the IBA 1 and Caspase 3 techniques for inflammation and potential apoptosis. Representative histological and histochemical photomicrographs were analysed using principles of qualitative histology and histochemistry. Results and Conclusion: Findings from the current research showed that RFR-exposure did not produce teratogenic or neurodegenerative effects within the hippocampus. This was evident from the study of the hippocampus’ histoarchitectural organisation, morphologies of the cells as well as their spatial distribution.  Functional integrity of cells in the different regions of the hippocampal formation, namely the CA 1-4 areas as well as the dentate gyrus also showed that Nissl substance expression, which is a marker of neuron functional integrity, was relatively normally expressed across the experimental animals.  This experimental modelling of human habitual exposure to RFR showed no evidence of prenatal teratogenic effects or postnatally induced extensive neurodegeneration up until puberty. However, it would be very important to indicate that RFR-exposure enhanced apoptotic potentials via the Caspase-3 pathway. The implications of this effect on later life mental health and neurological attributes will require further investigation

The effects of prenatal and postnatal exposure to electromagnetic field on rat ovarian tissue

2020 ◽  
Vol 36 (12) ◽  
pp. 1010-1018
Author(s):  
Acikgoz Burcu ◽  
Ersoy Nevin ◽  
Aksu Ilkay ◽  
Kiray Amac ◽  
Bagriyanik Husnu Alper ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Sham Group ◽  
Ovarian Tissue ◽  
Female Offspring ◽  
Postnatal Exposure ◽  
Pregnant Rats ◽  
Tissue Samples ◽  
Rat Pups ◽  
And Function ◽  
Oxide Synthase

Exposure to an electromagnetic field (EMF) can have adverse effects on many organs and tissues, including the reproductive system. This study aimed to investigate the effects of EMF exposure during prenatal and postnatal periods on ovarian development in rat offspring. In this study, rat pups born from eight pregnant rats were used. EMF exposure was initiated on the first day of pregnancy and continued until the 42nd postnatal day. The blood and ovarian tissue samples of female offspring in sham and EMF groups were collected when they reached the age of 42 days. Follicle-stimulating hormone levels were significantly higher in the EMF group than in the sham group. Estradiol levels were significantly lower in the EMF group than in the sham group. Tissue-inducible nitric oxide synthase (iNOS) levels and expression were significantly greater in the EMF group than in the sham group. In the EMF group, congestion, bleeding areas, and degeneration of follicle structures were observed in ovarian tissue. The findings suggest that exposure to 50-Hz, 3-mT EMF used in this study during prenatal and postnatal periods may lead to impaired ovarian structure and function in female offspring. EMF may affect ovarian physiology by increasing iNOS levels and may lead to fertility disorders.

Antitumoral and Anticholinesterasic Activities of the Seven Species from Rubiaceae

2019 ◽  
Vol 20 (4) ◽  
pp. 302-308 ◽  
Author(s):  
Carla R.F. Volobuff ◽  
Pedro C.O. Junior ◽  
Sidney M. dos Santos ◽  
Zefa V. Pereira ◽  
Diego C. Ferreira ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Biological Activities ◽  
Brain Structures ◽  
Acetylcholinesterase Inhibition ◽  
Mato Grosso ◽  
Male Wistar Rats ◽  
First Time ◽  
Mato Grosso Do Sul ◽  
Antiproliferative Activities ◽  
Mcf 7

Background: The genus Psychotria and Palicourea are reported as a source of alkaloids and iridoids, which exhibit biological activities. This study aimed to evaluate antiproliferative and anticholinesterase activities and quantification of the alkaloids of seven species among the genus found in Mato Grosso do Sul region in Brazil. Methods: Concentrations of alkaloids were measured spectrophotometrically. The extracts were submitted to antiproliferative activity against ten cell lines. The anticholinesterase activity of the extracts was developed using brain structures of male Wistar rats: cerebral cortex, hippocampus, hypothalamus and striatum by the Ellman method. Results: Alkaloids from Psychotria and Palicourea species were quantified which showed values of 47.6 to 21.9 µg/g. Regarding the antiproliferative potential, Palicourea crocea demonstrated selectivity against the 786-0 cell line (GI50: 22.87 µg/mL). Psychotria leiocarpa inhibited cell growth against OVCAR-3 (GI50: 3.28 µg/mL), K-562 (GI50: 5.26 µg/mL), HaCaT (GI50: 27.20 µg/mL), PC-3 (GI50: 34.92 µg/mL), MCF-7 (GI50: 35.80 µg/mL) and P. capillacea showed activity against OVCAR-3 (GI50: 2.33 µg/ml) and U251 (GI50: 16.66 µg/ml). The effect of acetylcholinesterase inhibition was more effective in the hippocampus, demonstrating inhibition for Paliourea crocea, Psychotria deflexa, P. brachybotrya and P. leiocarpa of 70%, 57%, 50% and 40%, respectively, followed by P. poeppigiana and P. capillacea, inhibiting 21%, compared to the control. Conclusion: Herein, the present work showed for the first time, anticholinesterasic and antiproliferative activities of extracts of Palicourea and Psychotria seem to be mainly associated with the levels of alkaloids in the leaves of these species.

scholarly journals Whole-brain 3D MR fingerprinting brain imaging: clinical validation and feasibility to patients with meningioma

2021 ◽  
Author(s):  
Thomaz R. Mostardeiro ◽  
Ananya Panda ◽  
Robert J. Witte ◽  
Norbert G. Campeau ◽  
Kiaran P. McGee ◽  
...  
Keyword(s):  
White Matter ◽  
Statistical Significance ◽  
Relaxation Times ◽  
Brain Structures ◽  
Centrum Semiovale ◽  
In Vivo Evaluation ◽  
Whole Brain ◽  
Mean Values ◽  
Caudate Head

Abstract Purpose MR fingerprinting (MRF) is a MR technique that allows assessment of tissue relaxation times. The purpose of this study is to evaluate the clinical application of this technique in patients with meningioma. Materials and methods A whole-brain 3D isotropic 1mm3 acquisition under a 3.0T field strength was used to obtain MRF T1 and T2-based relaxometry values in 4:38 s. The accuracy of values was quantified by scanning a quantitative MR relaxometry phantom. In vivo evaluation was performed by applying the sequence to 20 subjects with 25 meningiomas. Regions of interest included the meningioma, caudate head, centrum semiovale, contralateral white matter and thalamus. For both phantom and subjects, mean values of both T1 and T2 estimates were obtained. Statistical significance of differences in mean values between the meningioma and other brain structures was tested using a Friedman’s ANOVA test. Results MR fingerprinting phantom data demonstrated a linear relationship between measured and reference relaxometry estimates for both T1 (r2 = 0.99) and T2 (r2 = 0.97). MRF T1 relaxation times were longer in meningioma (mean ± SD 1429 ± 202 ms) compared to thalamus (mean ± SD 1054 ± 58 ms; p = 0.004), centrum semiovale (mean ± SD 825 ± 42 ms; p < 0.001) and contralateral white matter (mean ± SD 799 ± 40 ms; p < 0.001). MRF T2 relaxation times were longer for meningioma (mean ± SD 69 ± 27 ms) as compared to thalamus (mean ± SD 27 ± 3 ms; p < 0.001), caudate head (mean ± SD 39 ± 5 ms; p < 0.001) and contralateral white matter (mean ± SD 35 ± 4 ms; p < 0.001) Conclusions Phantom measurements indicate that the proposed 3D-MRF sequence relaxometry estimations are valid and reproducible. For in vivo, entire brain coverage was obtained in clinically feasible time and allows quantitative assessment of meningioma in clinical practice.

Visualisation of GABAergic neurons and synapses in the rat brain using immunohistochemistry for two forms of glutamate decarboxylase

2021 ◽  
Vol 21 (2) ◽  
pp. 63-73
Author(s):  
Valeria A. Razenkova ◽  
Dmitrii E. Korzhevskii
Keyword(s):  
Brain Tissue ◽  
Glutamate Decarboxylase ◽  
Brain Structures ◽  
Gabaergic Neurons ◽  
Laboratory Animals ◽  
Tissue Samples ◽  
Tissue Sections ◽  
Background Staining ◽  
The Central Nervous System ◽  
Rat Forebrain

BACKGROUND: Taking into account the importance of GABAergic brain system research and also the opportunity to achieve specific and accurate results in laboratory studies using immunohistochemical approaches, it seems important to have a reliable method of visualization GABA-synthesizing cells, their projections and synapses, for the morphofunctional analysis of GABAergic system both in normal conditions and in the experimental pathology. AIM: The aim of the study was to visualize analyze GABAergic neurons and synapses within rats brain using three different antibody types against glutamate decarboxylase and to identify the optimal conditions for reaction performing. MATERIALS AND METHODS: The study was performed on paraffin brain tissue sections of 5 adult Wistar rats. Immunohistochemical reactions using three antibody types against glutamate decarboxylase isoform 67 (GAD67) and glutamate decarboxylase isoform 65 (GAD65) were performed. Additional controls on C57/Bl6 mice and Chinchilla rabbits brain samples were also carried out. RESULTS: Antibodies used in the research made it possible to achieve high quality of GABAergic structures visualizing without increasing background staining. At the same time different antibody types are distinct in their efficacy to perform immunohistochemistry reaction on laboratory animal brain tissue samples. By performing additional controls, we discovered that there is necessary to adsorb secondary reagents immunoglobulins in order to eliminate nonspecific staining. It was found that GAD67 and GAD65 distribution in rat forebrain structures is different. It was stated that GAD67 immunohistochemistry most completely reveals GABAergic brain structures compared to GAD65 immunhistochemistry. The possibility of determining morphological features of GABAergic neurons and synaptic terminals, as well as performing quantitative analysis, was demonstrated. CONCLUSIONS: The approach proposed makes it possible to specifically visualize GABAergic structures of the central nervous system of different laboratory animals. This could be useful both in fundamental studies and in pathology research.

scholarly journals Effect of the oral administration of monosodium glutamate during pregnancy and breast-feeding in the offspring of pregnant Wistar rats

2010 ◽  
Vol 25 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Vinicius von Diemen ◽  
Manoel Roberto Maciel Trindade
Keyword(s):  
Weight Gain ◽  
Oral Administration ◽  
Wistar Rats ◽  
Monosodium Glutamate ◽  
The Other ◽  
Control Group ◽  
Pregnant Rats ◽  
Dependent Relation ◽  
Dose Dependent

PURPOSE: Determine the effects of the MSG (monosodium glutamate) in the offspring of pregnant rats through the comparison of the weight, NAL (nasal-anal length) and IL (Index of Lee) at birth and with 21 days of life. METHODS: Pregnant Wistar rats and their offspring were divided into 3 groups: GC, G10 and G20. Each of the groups received 0%, 10% and 20% of MSG, respectively from coupling until the end of the weaning period. RESULTS: Neither weight nor NAL were different among the groups at birth. The group G20 at birth had an IL lower than the group GC (p<0,05) and with 21 days of life presented weight and NAL lower than the groups G10 and this lower than the GC (p<0,01). Otherwise the G20 at 21 days of life had the IL similar to the other two groups. The weight profit percentage from birth to the 21st day of life was lower in the G20 regarding the other two groups (p<0,01). The G20 had a NAL increase percentage from birth to the 21st day of life lower than the G10 and this lower than the GC (p<0,01). CONCLUSIONS: MSG presented a dose-dependent relation in the variables weight and NAL. It caused a decrease in the growth pattern as well as in the weight gain pattern until the 21st day of life. The IL of the group 20% had an increased in relation to the control group after 3 weeks of follow up.

scholarly journals SPECTRAL ENTROPY FOR ASSESSING THE PATIENT RESPONSIVENESS DURING PROPOFOL/FENTANYL SEDATION IN DAY CARE SURGERIES

2019 ◽  
Vol 3 (9) ◽  
Author(s):  
Dr Shalendra Singh ◽  
Dr Priya Taank
Keyword(s):  
Day Care ◽  
Objective Assessment ◽  
Primary Objective ◽  
Sedation Scale ◽  
Depth Of Anaesthesia ◽  
Anesthetic Care ◽  
Mean Values ◽  
Day Care Surgery ◽  
Monitoring Of Sedation ◽  
Care Surgery

Background: For day care surgery under monitored anesthetic care, precise monitoring of sedation depth facilitates optimization of dosage and prevents adverse complications from over sedation. Conventionally subjective sedation scales, such as the Modified observer’s assessment of alertness/ sedation scale (MOAA/S) have been widely utilized for sedation monitoring. The newer monitoring called entropy is considered to be beneficial for objective assessment with combined use of opioids and hypnotics if applied. The primary objective is to determine measurement of entropy as a marker for measuring depth of anaesthesia. Methods: Two groups P and PF ( with 25 patients each) received either propofol 1mg/kg followed by maintenance infusion of 250 mic/ kg/hr whereas “PF”group received additional single dose of fentanyl 2 mic/kg respectively. The values of response entropy (RE) and state entropy (SE) corresponding to each MOAA/S (5 to 0) were determined. Results: The patient’s demographic profile and clinical characteristics were comparable in both the groups. No difference observed in duration of anaesthesia and surgery in both groups. No difference observed in MOAA/S in both groups.  The results shows a highly significant differences in the observed means of SE, RE, MAP and HR with considerably higher mean values in group P (p<0.0001). However other parameter such as SPO2, ETCO2 and RR were almost comparable in both groups. Conclusion: The mean value of SE and RE in group P and PF indicates that deeper plane of anaesthesia is observed in PF group. It is concluded that in assessing the level of hypnosis during intra-operative sedation in MAC, entropy corresponds to MOAA/S and increases or decreases proportionately depending upon increase or decrease level of sedation. Hence from these results it is proposed that entropy monitoring is a reliable monitoring index of anaesthesia depth in MAC. Keywords: Entropy, Propofol, Sedation, Day care surgery, Observer’s assessment of alertness/ sedation scale, Monitored anaesthesia Care

scholarly journals Effects of ligature-induced periodontitis in pregnant Wistar rats

2003 ◽  
Vol 17 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Mariane Ponzio de Azevedo Galvão ◽  
Cassiano Kuchenbecker Rösing ◽  
Maria Beatriz Cardoso Ferreira
Keyword(s):  
Body Weight ◽  
Birth Weight ◽  
Low Birth Weight ◽  
Periodontal Disease ◽  
Wistar Rats ◽  
The Body ◽  
Control Group ◽  
Female Adult ◽  
Pregnant Rats ◽  
Male Adult

The aim of this study was to evaluate the influence of ligature-induced periodontal disease in pregnant rats on their newborn's health parameters. Twenty-four female adult Wistar rats were divided into two groups: the control group (G1) and the group that was submitted to dental ligatures around second upper molars (G2). After the four week period of development of periodontitis, the female animals were mated with male adult Wistar rats. There were no differences in the body weight of females between the two groups during mating and pregnancy. No differences were observed among the groups in relation to the viable newborn index. However, there were differences in newborn birth weight, explained by the diverse size of the litters. In this study, ligature-induced periodontal disease did not promote changes during pregnancy that resulted in low birth weight in newborn Wistar rats.

Local intrarenal vasoconstrictor-vasodilator interactions in mild partial ureteral obstruction

1979 ◽  
Vol 236 (2) ◽  
pp. F131-F140 ◽  
Author(s):  
I. Ichikawa ◽  
B. M. Brenner
Keyword(s):  
Angiotensin Ii ◽  
Flow Rate ◽  
Ureteral Obstruction ◽  
Wistar Rats ◽  
Unilateral Ureteral Obstruction ◽  
Hydraulic Pressure ◽  
Mean Values ◽  
Glomerular Capillary ◽  
Single Nephron ◽  
Systemic Factor

Micropuncture studies were performed in Munich-Wistar rats with surgically created chronic partial unilateral ureteral obstruction (UUO). Mean values for superficial single nephron (SN)GFR, total GFR, and initial glomerular plasma flow rate (QA) in obstructed kidneys were essentially identical to values in nonobstructed kidneys. Nevertheless, glomerular capillary hydraulic pressure (PGC) was significantly higher in obstructed than in nonobstructed kidneys. This increase in PGC served to offset the markedly reduced glomerular capillary ultrafiltration coefficient that was also confined to the kidneys ipsilateral to the ureteral obstruction. During infusion of indomethacin or meclofenamate, SNGFR and QA decreased significantly, in association with elevations in arteriolar resistances in obstructed kidneys, whereas such changes were not observed in nonobstructed kidneys. The results suggest that local intrarenal factors, rather than circulating or systemic factor(s), bring about functional adaptations to partial ureteral obstruction. In particular, an indomethacin- and meclofenamate-sensitive vasodilator (presumably prostaglandin) plays a role in antagonizing the effects of a simultaneously acting vasoconstrictor which, although not identified, displayed the functional properties of angiotensin II.

Sign in / Sign up

Export Citation Format

Share Document