Antimicrobial and Cytotoxic Activities and GC-MS Analysis of Phytocomponents of Methanolic Extract of Curculigo pilosa (Schum and Thonn) Engl. (Hypoxidaceae) Rhizomes

2014 ◽  
Vol 4 (12) ◽  
pp. 1552-1567 ◽  
Author(s):  
Elijah Shaba
Keyword(s):  
Methanolic Extract ◽  
Cytotoxic Activities ◽  
Ms Analysis

GC-MS ANALYSIS OF PHYTOCHEMICALS IN THE METHANOLIC EXTRACT OF Caesalpinia coriaria (Jacq) Willd.

2016 ◽  
Vol 4 (7) ◽  
pp. 1417-1422 ◽  
Author(s):  
P.T. Srinivasan ◽  
◽  
D. Anandhi ◽  
S. Kanimozhi ◽  
M. Anbarasan. ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Ms Analysis

scholarly journals UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca

2021 ◽  
Vol 72 (1) ◽  
pp. 97-108
Author(s):  
Rafeef K. Massadeh ◽  
Tamam El-Elimat ◽  
Mohammad Al-Gharaibeh ◽  
Khaled Tawaha ◽  
Feras Q. Alali
Keyword(s):  
High Resolution ◽  
Plant Material ◽  
Methanolic Extract ◽  
Volatile Oil ◽  
Ethyl Linoleate ◽  
Major Constituent ◽  
Hexadecanoic Acid ◽  
Nicotiana Glauca ◽  
Plant Oil ◽  
Ms Analysis

Abstract The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g−1 dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of N. glauca. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (e.g., β-bisabolol) and carboxylic acids and esters (e.g., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.

Eugenol Found by GC-MS Analysis of The Methanolic Extract of The Fruit Pulp of Indigenous Mango (Mangifera indica L.) Variety, Champa from Murshidabad District of West Bengal, India

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Mitu De ◽  
Susanta Ray ◽  
Subhasree Dutta ◽  
Santi Ranjan Dey
Keyword(s):  
Volatile Compounds ◽  
West Bengal ◽  
Methanolic Extract ◽  
Mangifera Indica ◽  
Fruit Pulp ◽  
Ms Analysis ◽  
Bioactive Component ◽  
Herbal Plants ◽  
Michelia Champaca

Indigenous mango (Mangifera indica L.) varieties have unique aroma, taste, flavour, texture and size. The interactions among volatile compounds and with sugars and acids have a role in the distinct mango flavor. Murshidabad district of West Bengal has several indigenous mango varieties that have unique attributes like taste and aroma. The indigenous Champa mango variety of Murshidabad district has a distinct aroma very similar to that of the champaka flower (Michelia champaca L.). The present investigation was to determine if there was any phyto-chemical which could be responsible for the distinct and unique aroma present in the mango variety Champa. Eugenol, a phytogenic bioactive component frequently found in diversified herbal plants was found by GC-MS analysis of the methanolic extract of the fruit pulp of indigenous mango variety, Champa.

scholarly journals GC-MS analysis, antimicrobial, antioxidant, antilipoxygenase and cytotoxic activities of Jacaranda mimosifolia methanol leaf extracts and fractions

PLoS ONE ◽  
2020 ◽  
Vol 15 (7) ◽  
pp. e0236319 ◽  
Author(s):  
Rabia Naz ◽  
Thomas H. Roberts ◽  
Asghari Bano ◽  
Asia Nosheen ◽  
Humaira Yasmin ◽  
...  
Keyword(s):  
Cytotoxic Activities ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Jacaranda Mimosifolia

scholarly journals Decorosides A and B, Cytotoxic Flavonoid Glycosides from the Leaves of Rhododendron decorum

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Mostafa E. Rateb ◽  
Hossam M. Hassan ◽  
El-Shaimaa A. Arafa ◽  
Marcel Jaspars ◽  
Rainer Ebel
Keyword(s):  
Spectral Data ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Methanolic Extract ◽  
Cancer Cell Lines ◽  
Biological Evaluation ◽  
Flavonoid Glycosides ◽  
Cytotoxic Activities ◽  
Diphenyltetrazolium Bromide

Bioassay and NMR-guided fractionation of the methanolic extract of Rhododendron decorum leaves resulted in the isolation of two new flavonoid glycosides, 5,7-dihydroxy-6,8-dimethyldihydroflavanone-7-O-α-L-arabinopyranosyl(l→6)-β-D-glucopyranoside (decoroside A, 1) and its 3-hydroxy congener (decoroside B, 2), along with five known compounds myricitrin (3), afzelin (4), (-)-epicatechin (5), (+)-catechin (6), and ampeloptin (7). The structures of the isolated compounds were elucidated by extensive interpretation of their spectral data. Biological evaluation using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay revealed promising cytotoxic activities of these compounds against different cancer cell lines.

Trigona laeviceps Propolis from Thailand: Antimicrobial, Antiproliferative and Cytotoxic Activities

2009 ◽  
Vol 37 (05) ◽  
pp. 855-865 ◽  
Author(s):  
Supawadee Umthong ◽  
Songchan Puthong ◽  
Chanpen Chanchao
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Water Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
E Coli ◽  
Anticancer Properties ◽  
Crude Extracts ◽  
Sw620 Cells

Propolis is one of the natural bee products which has long been used as a crude preventative and prophylactic medicine, and has been reported to possess antibacterial, antiviral, anti-inflammatory, antioxidative and anticancer properties. Propolis of the stingless bee, Trigona laeviceps, was extracted by water or methanol at 35% (w/v) yielding a crude water or a methanolic extract at 60 and 80 mg/ml, respectively, which is 17.1 and 22.9% (w/w) of the total propolis, respectively. The antimicrobial activity of both crude extracts was assayed on four selected pathogenic microbes by using the agar well diffusion method. The results suggested that both water and methanolic crude extracts have some antimicrobial activities, water extract has greater antimicrobial activity than methanolic extract. The relative order of sensitivity of the four microbes were, however, the same between the two extracts from the most to least sensitive, S. aureus > E. coli ≫ C. albicans ⋙ A. niger, with indeed no observed growth inhibition of A. niger at all. Antiproliferative and cytotoxic affects were tested on the colon carcinoma cell line, SW620, using the three parameters: (1) MTT assay; (2) cell morphology; and (3) the fragmentation of genomic DNA. The water extract of propolis showed a higher antiproliferative activity than that of methanolic extract to SW620 cells, additionally both appeared to cause cell death by necrosis.

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