A Study on Nutritional Composition and GC-MS Phytochemical Analysis of Thaumatococcus daniellii Leaves

Aims: There is a high risk associated with using artificial materials like polyethylene or foil for wrapping foods before cooking. The present study investigated the phytochemical and nutrient composition of Thaumatococus daniellii leaf, a local food wrapper. Place and Duration of Study: Biochemistry Unit, Department of Science Laboratory Technology, Imo State Polytechnic Umuagwo, between April and May 2018. Methodology: The phytochemical compounds were analyzed using gas chromatography coupled with mass spectrometry (GC-MS) while proximate and vitamin composition was determined using standard analytical methods. Results: The GC-MS phytochemical analysis revealed the presence of eight (8) bioactive compounds 1,2,3,4-Butanetetrol, d-Glycero-d-idoheptose, Cyclopentane,1-ethyl-1-methyl-, 6-Octyl-1-ol,3,7-dimethyl-, 2-Octenal, E- Octane,1-chloro-,2-Acetoxy-1,1,10-trimethyl-6,9-epidioxydecalin and Bicyclo[3.1.1]heptan-3-ol,2,6,6-trimethyl-,(1α,2β,3α,5α)- while vitamin assay results showed that Thaumatococcus daniellii leaf contained 3.1mg/100g of vitamin A, 1.07 mg/100 g vitamin B1, 1.32 mg/100 g vitamin B3, 1.11 mg/100 g of vitamin B5, 16.34 mg/100 g of vitamin B6, 11.86 mg/100 g of vitamin B12 and 25.19 mg/100 g of vitamin C. Result of proximate analysis indicates that Thaumatococcus daniellii leaf contains 10.15% ash, 9.67% moisture, 20.41% protein, 11.42% lipids, 13.78% fibre and 34.57% carbohydrate. Conclusion: In summary, our findings indicate that Thaumatococcus daniellii leaves contain bioactive compounds, an appreciable number of vitamins and high nutritional constituents. Hence Thaumatococcus daniellii leaves can be incorporated into animal feed, taking into cognizance the high protein, fat and vitamin contents. These suggest that it may play critical medicinal and nutritional roles and probably be a safer alternative to polyethylene paper and aluminum foil wraps; hence justifying its local usage as food wrapper. The leaves may also serve as a lead for the production of a healthier material for wrapping local foods.

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Phytochemical Analysis of the Fruits of Sea Buckthorn (Hippophae rhamnoides): Identification of Organic Acid Derivatives

Plants ◽

10.3390/plants10050860 ◽

2021 ◽

Vol 10 (5) ◽

pp. 860

Author(s):

Yong Hoon Lee ◽

Hee Joo Jang ◽

Kun Hee Park ◽

Seon-Hee Kim ◽

Jung Kyu Kim ◽

...

Keyword(s):

Organic Acid ◽

Negative Control ◽

Nutritional Composition ◽

Sea Buckthorn ◽

Hippophae Rhamnoides ◽

Phytochemical Analysis ◽

Bioactive Constituents ◽

Hippophaë Rhamnoides ◽

The Absolute ◽

New Compounds

Hippophae rhamnoides L. (Elaeagnaceae), commonly known as “Sea buckthorn” and “Vitamin tree”, is a spiny deciduous shrub whose fruit is known for its nutritional composition, such as vitamin C, and is consumed as a dietary supplement worldwide. As part of our ongoing efforts to identify structurally new and bioactive constituents from natural resources, the phytochemical investigation of the extract of H. rhamnoides fruits led to the isolation of one malate derivative (1), five citrate derivatives (2–6), and one quinate derivative (7). The structures of the isolated compounds were elucidated by analysis of 1D and 2D nuclear magnetic resonance (NMR) spectroscopic data and high-resolution electrospray ionization (HR-ESI) liquid chromatography–mass spectrometry (LC/MS) data. Three of the citrate derivatives were identified as new compounds: (S)-1-butyl-5-methyl citrate (3), (S)-1-butyl-1′-methyl citrate (4), and (S)-1-methyl-1′-butyl citrate (6), which turned out to be isolation artifacts. The absolute configurations of the new compounds were established by quantum chemical electronic circular dichroism (ECD) calculation, which is an informative tool for verifying the absolute configuration of organic acid derivatives. The isolated compounds 1–7 were evaluated for their stimulatory effects on osteogenesis. Compounds 1, 3, 4, 6, and 7 stimulated osteogenic differentiation up to 1.4 fold, compared to the negative control. These findings provide experimental evidence that active compounds 1, 3, 4, 6, and 7 induce the osteogenesis of mesenchymal stem cells and activate bone formation.

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Phytochemical Analysis and Antiradical Properties of Sarcodon imbricatus (L.:Fr) Karsten

Natural Product Communications ◽

10.1177/1934578x0800301123 ◽

2008 ◽

Vol 3 (11) ◽

pp. 1934578X0800301 ◽

Cited By ~ 2

Author(s):

Maria Carla Marcotullio ◽

Gildas Norbert Oball-Mond Mwankie ◽

Lina Cossignani ◽

Bruno Tirillini ◽

Rita Pagiotti

Keyword(s):

Antiradical Activity ◽

Unsaturated Fatty Acids ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Nutritional Composition ◽

Phytochemical Analysis ◽

Ergosterol Peroxide ◽

Antiradical Properties ◽

Dpph Radical Scavenging ◽

Good Food

The chemical composition of Sarcodon imbricatus (L.:Fr) Karsten (Hydnaceae) was evaluated to assess it as source of nutrients and nutraceuticals. The analyzed mushroom contains ergosterol and ergosterol peroxide. S. imbricatus methanolic extract showed a moderate antiradical activity (measured by DPPH radical scavenging activity). The combination of bioactive compounds and rich nutritional composition (high contents in protein, low content in fat, and its content of unsaturated fatty acids) makes the mushroom a good food.

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Phytochemical Analysis, Nutritional Composition and Antimicrobial Activities of White Mulberry (Morus alba)

Pakistan Journal of Nutrition ◽

10.3923/pjn.2012.456.460 ◽

2012 ◽

Vol 11 (5) ◽

pp. 456-460 ◽

Cited By ~ 8

Author(s):

M.O. Omidiran ◽

R.A. Baiyewu ◽

I.T. Ademola ◽

O.C. Fakorede ◽

E.O. Toyinbo ◽

...

Keyword(s):

Antimicrobial Activities ◽

Nutritional Composition ◽

Morus Alba ◽

Phytochemical Analysis ◽

White Mulberry

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Evaluation of nutritional, phytochemical, antioxidant and cytotoxic potential of Capsella bursa-pastoris, a wild vegetable from potohar region of Pakistan

Kuwait Journal of Science ◽

10.48129/kjs.v48i3.9562 ◽

2021 ◽

Vol 48 (3) ◽

Author(s):

Iqra Riaz ◽

◽

Yamin Bibi ◽

Nabeela Ahmad ◽

Sobia Nisa ◽

...

Keyword(s):

Antioxidant Activity ◽

Brine Shrimp ◽

Methanol Extract ◽

Reducing Power ◽

Nutritional Composition ◽

Total Phenolic ◽

Human Consumption ◽

Phytochemical Analysis ◽

Cytotoxic Potential ◽

Dpph Method

Capsella bursa-pastoris has been analyzed for nutritional composition, antioxidant and cytotoxic potential to reveal its nutraceutical and medicinal importance. Methanol and chloroform extracts were used for preliminary phytochemical analysis following quantification of total phenolic, flavonoid, tannins, saponins, and alkaloids. Antioxidant activity was determined by using DPPH, phosphomolybdate, and reducing power assays. Proximate and elemental analysis was performed by using standard methods. Brine shrimp larvae assay was used to determine the cytotoxicity of chloroform and methanol extract. Chloroform and methanol extract of the plant indicated the presence of 1.56×10-4 mg g-1 and 1.28×10-4 mg g-1 phenolic, respectively. The plant was found to have high carbohydrate and protein contents. The high-level mineral composition was determined containing Potassium, Sodium, Iron, Zinc, and Copper. Results of antioxidant activity by the DPPH method indicated IC50 of 235.37 μg ml-1 and 552.01 μg ml-1 for chloroform and methanol extract, respectively. Brine shrimp larvae assay was used to determine the cytotoxicity resulting LD50 of 246.4469 μg ml-1 and 169.85 μg ml-1 of methanol and chloroform extract, respectively. The study inferred that C. bursa-pastoris could serve as a good source of protein and energy as well as micronutrients in the form of a leafy vegetable for human consumption.

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Comparative study of composition, antioxidant and antimicrobial activity of two adult edible insects from Tenebrionidae family

BMC Chemistry ◽

10.1186/s13065-020-00707-0 ◽

2020 ◽

Vol 14 (1) ◽

Author(s):

Daniel R. Flores ◽

Luz E. Casados ◽

Sandra F. Velasco ◽

Ana C. Ramírez ◽

Gilberto Velázquez

Keyword(s):

Antioxidant Activity ◽

Protein Extraction ◽

Antimicrobial Activities ◽

Nutritional Composition ◽

Protein Hydrolysates ◽

Phytochemical Analysis ◽

Protein Extract ◽

Lack Of Information ◽

Microbiological Control ◽

Antioxidant And Antimicrobial Activity

Abstract In the case of Tenebrionidae family insects, studies focus on larval stage, leaving a lack of information regarding other stages. Therefore, this study was performed in order to understand the differences between the nutritional composition and the bioactivity of two species of this family in their adult stage, fed with a specific diet. Adult beetles of both species were defatted, lyophilized and protein extracted with buffer. Proximal and phytochemical analysis of the extracts of each insect were performed, along with protein extract and hydrolysis analysis by Tris-Tricine and Tris Glycine SDS PAGE. This analysis showed that T. molitor contained more protein and fat than U. dermestoides but contained less crude fiber. The protein extraction was made with PBS, where 130 and 45 kDa bands showed predominant for U. dermestoides, and less protein was present for T. molitor. Antioxidant and antimicrobial activities of the enzymatic protein hydrolysates and protein crude extracts were determined. Presence of protein associated with the antioxidant activity were found in both insects. Nonetheless U. dermestoides had a higher antioxidant activity with the protein extract in contrast with the higher antioxidant activity shown by U. dermestoides once the extracts were digested. After proteolysis, protein extracts showed an increasing antioxidant activity, plus, the ability to inhibit microbial growth of Proteus, Shigella and Bacillus. Insect protein hydrolysates with protease open the possibility for the use of these beetles as new sources of encrypted peptides for microbiological control once characterized.

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Nutritional Composition and Phytochemical Analysis of Aqueous Extract of Allium cepa (Onion) and Allium sativum (Garlic)

Asian Food Science Journal ◽

10.9734/afsj/2018/43165 ◽

2018 ◽

Vol 3 (4) ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Mahmood Hassan Dalhat ◽

Fatima Abdulkadir Adefolake ◽

Maimuna Musa

Keyword(s):

Allium Cepa ◽

Aqueous Extract ◽

Allium Sativum ◽

Nutritional Composition ◽

Phytochemical Analysis

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Evaluation of Phytochemical Contents, Proximate Nutritional Composition and Antimicrobial Activity of the Leaves and Rhizome Extracts of Cyperus rotundus Linn. in Uyo, Akwa Ibom State, Nigeria

South Asian Journal of Research in Microbiology ◽

10.9734/sajrm/2020/v7i130159 ◽

2020 ◽

pp. 1-11

Author(s):

Ikon, Grace Michael ◽

Etang, Ubong Ekerenam ◽

Udoiko, Etima Micah ◽

Ohagim, Ifunanya Promise

Keyword(s):

Antimicrobial Activity ◽

Plant Extracts ◽

Test Organism ◽

Nutritional Composition ◽

Cyperus Rotundus ◽

Phytochemical Analysis ◽

Aqueous Extracts ◽

Plant Materials ◽

Zones Of Inhibition ◽

Phytochemical Contents

Background: The leaves and rhizome extracts of Cyperus rotundus Linn. popularly called “Nut grass” in many Nigerian communities have been extensively used in local food preparation and in treatment purposes. Aim: This study aimed to evaluate the phytochemical contents, proximate nutritional composition and antimicrobial activity of the leaves and rhizome extracts of C. rotundus. Methodology: The disease-free plant materials were collected from a farm in Uyo, Akwa Ibom State, Nigeria. Preparation of the plant material, methanolic and aqueous extracts; bacterial culture, isolation, microscopy and biochemical identification; phytochemical screening and proximate nutritional analysis were done according to standard methods, while screening for antimicrobial activity was done by agar well diffusion technique. Results: The preliminary phytochemical analysis of the plant extracts showed the presence of bioactive compounds at varying amounts such as glycosides, tannins, reducing sugars, alkaloids, flavonoids, polyphenols, terpenoids, saponins and phlobatannins. The proximate nutritional and elemental analysis of C. rotundus extracts showed high presence of B-carotene (164.3 ± 0.02), Vitamin A (109.25 ± 0.01) and carbohydrate (59.0 ± 0.01) with moderate content of lipid (24.25 ± 0.02) and moisture (9.10 ± 0.01) as well as contents of some mineral elements such as Ca, K and P occurring in the range literature values in mg per 100 g dry weight of the plant sample. The methanol and aqueous extracts of C. rotundus showed varying diameter of zones of inhibition on the test organism. The observable inhibitory effect of the plant extracts on the test organism was more pronounced with methanol extracts as indicated by the diameter of zones of inhibition in mm in the order of 22.0>14.0>13.0 for P. mirabilis, E. coli and S. aureus, respectively compared to the aqueous extract. Conclusion: The results of this study have shown the antimicrobial, therapeutic and nutritional potential of the leaves and rhizome extracts of C. rotundus. It could possibly find application as a good alternative antibacterial agent, nutraceuticals and dietary supplements.

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