scholarly journals Determination of PRSV Tolerant Papaya Plants from SBF6 Generation of Intergeneric Population Using Ranking Model System

2020 ◽  
pp. 133-139
Author(s):  
R. Mohammed Aseef ◽  
K. Soorianathasundaram ◽  
P. Paramaguru ◽  
P. Muthulakshmi ◽  
A. John Joel ◽  
...  
Keyword(s):  
Genetic Parameters ◽  
Selection Process ◽  
Model System ◽  
Disease Index ◽  
Ranking Model ◽  
Number Of Leaves ◽  
The Mean ◽  
Cent Disease ◽  
Variability Study

The Full-sib mated sixth (SBF6) generation of intergeneric population of papaya from the cross CP 50 x Vasconcellea cauliflora was evaluated to select the progenies with PRSV tolerance coupled with better yield. In order to identify the well performing progenies, the mean performance and genetic parameters were estimated. Interestingly, the results of the variability study revealed that the genotypic coefficient of variation on number of fruits, leaf area and number of leaves were higher and making it as the selectable traits. The correlation analysis was made to understand the degree of relationship between the PRSV tolerance and various morphological traits. The result showed that plant height and number of fruits were significantly and negatively correlated with per cent disease index (PDI) with the r value of -0.273 and -0.261 respectively. By considering the number of fruits as the selectable trait, a ranking model system was developed and the results showed 5 ranks of 53 progenies. Among them, 13 well performing progenies from Rank IV and Rank V were selected for further evaluation. The ranking model system helps the researchers by making the selection process statistically much easier and efficient.

scholarly journals GENETIC EVALUATION OF SEED GERMINATION AND DEVELOPMENT OF SEEDLINGS IN Ormosia excelsa Benth

FLORESTA ◽  
2018 ◽  
Vol 48 (3) ◽  
pp. 331
Author(s):  
Jennifer Souza Tomaz ◽  
Maria Teresa Gomes Lopes ◽  
Mágno Sávio Ferreira Valente ◽  
Manuel De Jesus Vieira Lima Júnior ◽  
Graciela Inês Bolzón de Muniz ◽  
...  
Keyword(s):  
Genetic Parameters ◽  
Selection Process ◽  
Germination Rate ◽  
Different Temperatures ◽  
High Heritability ◽  
Mean Germination Time ◽  
Number Of Leaves ◽  
Set Up ◽  
Germination Traits ◽  
Selection Of

The aim of this study was to estimate the genetic parameters of Ormosia excelsa seeds based on germination traits, at different temperatures, in order to facilitate the selection of superior varieties of seeds for producing seedlings. Twenty six progenies collected in the municipality of Autazes (state of Amazonas, Brazil) were evaluated. The experiment was completely randomized, with four replications and 25 seeds per plot, totaling 100 seeds per progeny. The germination rate, mean germination time and the synchronization and speed germination indexes were determined at the temperatures of 30 and 35 °C. Seedling development was also evaluated under nursery conditions. The progenies of O. excelsa showed significant genetic variability for all traits. Both temperatures proved to be effective in producing germination close to 87%. Germination began between 1 and 4 days after sowing. However, for greater efficiency in the selection of superior progenies, each environment should be considered individually. The high heritability values obtained (> 65%) and the high genetic correlation favorable for selection, among all traits, resulted in significant gains according to the selection process used. Our results indicated that determining superior progenies for height and number of leaves was possible based on a single evaluation. The fact that 100% of seedlings planted in the field survived promotes a promising outlook to set up commercial nurseries for the species.

Detection of Circulating Tissue Factor and Factor VII in a Normal Population

1996 ◽  
Vol 75 (05) ◽  
pp. 772-777 ◽  
Author(s):  
Sybille Albrecht ◽  
Matthias Kotzsch ◽  
Gabriele Siegert ◽  
Thomas Luther ◽  
Heinz Großmann ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Normal Population ◽  
Mean Value ◽  
Factor Vii ◽  
Significant Difference ◽  
Age Dependent ◽  
The Mean ◽  
Highly Correlated ◽  
And Gender

SummaryThe plasma tissue factor (TF) concentration was correlated to factor VII concentration (FVIIag) and factor VII activity (FVIIc) in 498 healthy volunteers ranging in age from 17 to 64 years. Immunoassays using monoclonal antibodies (mAbs) were developed for the determination of TF and FVIIag in plasma. The mAbs and the test systems were characterized. The mean value of the TF concentration was 172 ± 135 pg/ml. TF showed no age- and gender-related differences. For the total population, FVIIc, determined by a clotting test, was 110 ± 15% and the factor VIlag was 0.77 ± 0.19 μg/ml. FVII activity was significantly increased with age, whereas the concentration demonstrated no correlation to age in this population. FVII concentration is highly correlated with the activity as measured by clotting assay using rabbit thromboplastin. The ratio between FVIIc and FVIIag was not age-dependent, but demonstrated a significant difference between men and women. Between TF and FVII we could not detect a correlation.

Determination of Plasminogen in Clots and Thrombi

1966 ◽  
Vol 16 (01/02) ◽  
pp. 038-050 ◽  
Author(s):  
Ulla Hedner ◽  
Inga Marie Nilsson ◽  
B Robertson
Keyword(s):  
Mean Value ◽  
Main Mechanism ◽  
Clot Lysis ◽  
Post Mortem ◽  
Digestion Time ◽  
Normal Volunteers ◽  
Casein Solution ◽  
The Mean

SummaryThe plasminogen content was determined by a casein method in plasma and serum from 20 normal volunteers. The mean plasminogen content was found to be 10.1 ACU (the arbitrary caseinolytic unit defined in such a way that using a 3% casein solution and a digestion time of 20 min. at 37°C, 10 ACU gave an extinction of 0.300). No difference between serum and plasma regarding the plasminogen content was found.Plasminogen was determined in drained and drained plus washed clots prepared from 2 ml plasma. The highest values found in the drained clots were 0.9 ACU/clot and 0.2 ACU/clot in the drained plus washed clots.Plasminogen was also determined in drained and drained plus washed clots prepared from plasma with added purified plasminogen. The plasminogen was recovered in the washing fluid. According to these tests, then, purified added plasminogen is washed out of the clots.The plasminogen content of 20 thrombi obtained post mortem was also determined. The mean value was found to be 0.7 ACU/cm thrombus. Judging from our results, the “intrinsic clot lysis theory” is not the main mechanism of clot dissolution.

SERUM TRIIODOTHYRONINE DETERMINATION BY SATURATION ANALYSIS

1973 ◽  
Vol 72 (4) ◽  
pp. 714-726 ◽  
Author(s):  
A. Burger ◽  
B. Miller ◽  
C. Sakoloff ◽  
M. B. Vallotton
Keyword(s):  
Ionic Strength ◽  
Limit Of Detection ◽  
Improved Method ◽  
Accuracy Of Measurement ◽  
Tracer Amount ◽  
The Mean ◽  
Physiological Values ◽  
Hyperthyroid Patients ◽  
Solvent Blank

ABSTRACT An improved method for the determination of serum triiodothyronine (T3) has been developed. After addition of a tracer amount of the hormone, T3 was extracted from 1 ml serum under conditions of pH and ionic strength which favoured T3 extraction (89%) over thyroxine (T4) extraction (58%). Chromatography of the extracted material on Sephadex LH-20 separated T3 completely from residual T4. The T3 eluate was dried, then re-dissolved in 0.5 ml NaOH 0.04 n. To 0.2 ml duplicate aliquots, a standard amount of TBG was added for the competitive protein analysis. After one hour incubation at 4°C, separation of bound from free T3 was achieved on small Sephadex G-25 columns. Overall recovery was 67 ± 10.8% and correction for the loss was made. The solvent blank was 37 ± 27 (sd) ng/100 ml. Accuracy of measurement of known quantities of T3 added to serum was 98.4%. The coefficient of variation within the assay was 6.2% and between the assays it was 11.4%. The limit of detection (0.1 ng) corresponded to a concentration of 25 ng/100 ml. T4 added to serum did not interfere with T3 determination until high non-physiological values were reached. The mean ± sd serum T3 in 54 euthyroid subjects was 153 ± 58 ng/100 ml and in 24 hyperthyroid patients it was 428 ±186 ng/100 ml; 4 out of the 24 hyperthyroid values were within 2 sd of the mean euthyroid group. All the values found in the euthyroid group were well above the limit of detection of the method.

Determination of the Probability Distribution of the Mean Sound Level

2010 ◽  
Vol 35 (4) ◽  
pp. 543-550 ◽  
Author(s):  
Wojciech Batko ◽  
Bartosz Przysucha
Keyword(s):  
Probability Distribution ◽  
Probability Distribution Function ◽  
Mean Value ◽  
Sound Level ◽  
Function Form ◽  
Noise Levels ◽  
Logarithmic Mean ◽  
The Mean ◽  
Estimation Of Uncertainty

AbstractAssessment of several noise indicators are determined by the logarithmic mean <img src="/fulltext-image.asp?format=htmlnonpaginated&src=P42524002G141TV8_html\05_paper.gif" alt=""/>, from the sum of independent random resultsL1;L2; : : : ;Lnof the sound level, being under testing. The estimation of uncertainty of such averaging requires knowledge of probability distribution of the function form of their calculations. The developed solution, leading to the recurrent determination of the probability distribution function for the estimation of the mean value of noise levels and its variance, is shown in this paper.

Simultaneous Determination of Alogliptin, Linagliptin, Saxagliptin, and Sitagliptin in Bulk Drug and Formulation by UPLC Q-TOF-MS

2020 ◽  
Vol 17 (1) ◽  
pp. 95-105
Author(s):  
Ramji Rathod ◽  
Faraat Ali ◽  
Amrish Chandra ◽  
Robin Kumar ◽  
Meenakshi Dahiya ◽  
...  
Keyword(s):  
Internal Standard ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Pharmaceutical Dosage Forms ◽  
Sensitivity And Selectivity ◽  
The Mean ◽  
Bulk Drug ◽  
Positive Ion ◽  
Higher Sensitivity

Background: A simple and sensitive Ultra Performance Liquid Chromatography-Mass Spectrometry method was developed and validated to measure the concentrations of Alogliptin (ALO), Linagliptin (LIN), Saxagliptin (SAX), and Sitagliptin (SIT) using Pioglitazone (PIO) as an internal standard. Methods: Chromatographic separation of six gliptins was achieved on a C-18 column (100×2.1 mm, 2.7 μm) using a mobile phase consisting of formic acid in water, 0.1%v/v: acetonitrile in gradient elution. Electrospray ionization (ESI) source was operated in the positive ion mode. Targeted MS/MS mode on a QTOF MS was used to quantify the drug utilizing the transitions of 340.1(m/z), 473.2 (m/z), 316.2 (m/z), 408.1 (m/z), and 357.1 (m/z) for ALO, LIN, SAX, SIT and PIO respectively. Results: As per ICH Q2R1 guidelines, a detailed validation of the method was carried out and the standard curves were found to be linear over the concentration ranges of 1516.0-4548.1 ng mL-1, 519.8- 1559.4 ng mL-1, 1531.4-4594.3 ng mL-1and 1519.6-4558.8 ng mL-1 for ALO, LIN, SAX and SIT respectively. Precision and accuracy results were within the acceptable limits. The mean recovery was found to be 98.8 _ 0.76 % (GEM), 102.2 _ 1.59 % (LIN), 95.3 _ 2.74 % (SAX) and 99.2 _ 1.75 % (SIT) respectively. Conclusions: The optimized validated UPLC QTOF-MS/MS method offered the advantage of shorter analytical times and higher sensitivity and selectivity. The optimized method is suitable for application in quantitative analysis of pharmaceutical dosage forms for QC laboratory.

The flow of liquid in a stream from the standard turbine impeller

1979 ◽  
Vol 44 (3) ◽  
pp. 700-710 ◽  
Author(s):  
Ivan Fořt ◽  
Hans-Otto Möckel ◽  
Jan Drbohlav ◽  
Miroslav Hrach
Keyword(s):  
Reynolds Number ◽  
Kinematic Viscosity ◽  
Momentum Flux ◽  
Relative Size ◽  
Mean Velocity ◽  
Axial Profile ◽  
The Mean ◽  
Hot Film ◽  
Turbine Impeller

Profiles of the mean velocity have been analyzed in the stream streaking from the region of rotating standard six-blade disc turbine impeller. The profiles were obtained experimentally using a hot film thermoanemometer probe. The results of the analysis is the determination of the effect of relative size of the impeller and vessel and the kinematic viscosity of the charge on three parameters of the axial profile of the mean velocity in the examined stream. No significant change of the parameter of width of the examined stream and the momentum flux in the stream has been found in the range of parameters d/D ##m <0.25; 0.50> and the Reynolds number for mixing ReM ##m <2.90 . 101; 1 . 105>. However, a significant influence has been found of ReM (at negligible effect of d/D) on the size of the hypothetical source of motion - the radius of the tangential cylindrical jet - a. The proposed phenomenological model of the turbulent stream in region of turbine impeller has been found adequate for values of ReM exceeding 1.0 . 103.

Precipitation and filtration of zinc, magnesium, copper and aluminium hydroxides

1982 ◽  
Vol 47 (12) ◽  
pp. 3362-3370
Author(s):  
Otakar Söhnel ◽  
Eva Matějčková
Keyword(s):  
Residence Time ◽  
Mean Residence Time ◽  
Filtration Resistance ◽  
Filtration Pressure ◽  
Bed Porosity ◽  
The Mean ◽  
Filtration Properties

Filtration properties of batchwise precipitated suspensions of Zn(OH)2, Mg(OH)2 and Cu(OH)2 and continuously precipitated Al(OH)3 were studied. For batchwise precipitated suspensions was verified the theoretically predicted dependence of specific filtration resistance on initial supersaturation and for the continuously precipitated Al(OH)3 the relation between the specific filtration resistance and the mean residence time of suspension in the reactor. Dependences were also recorded between the bed porosity and concentration of precipitated solutions, specific filtration resistance and used filtration pressure and the effect of aging of the batchwise precipitated suspension of Mg(OH)2on its filtration properties. The used CST method for determination of filtration characteristics of Zn(OH)2 suspension was also studied.

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