Propagation Studies in Passion Fruit (Passiflora edulis Sims.) Using Cuttings

Passion fruit is a high value export oriented crop, which is gaining popularity among farmers. One of the constraints in expansion of this crop is availability of quality planting material. Passion fruit is propagated through seed, cutting and grafting. Among these methods, cuttings encourage the production and multiplication of true to type planting material. Therefore, a study was conducted at the Fruits Crops Research Station, Kerala Agricultural University, Vellanikkara, Thrissur, during August 2019 - November 2019 to evaluate the effect of different concentrations of NAA and number of nodes on the rooting of cuttings and survival percentage. The experiment was laid out in completely randomized design with twenty treatments and two replications. Experiment consisted of two factors, viz. NAA concentration (200 ppm, 400 ppm, 600 ppm, 800 ppm and control) and number of nodes (one node, two node, three node and four node), following quick dip method. The study revealed that there was significant variation among the treatments. When the effect of number of nodes alone was considered, four noded cutting had the maximum survival percentage, and higher root and shoot parameters. Improved survival percentage, and enhanced root and shoot characteristics were noticed with the increasing NAA concentration. From the present study, it was concluded that combined effect of more number of nodes and higher NAA concentration had profound effect on survival percentage, shoot length, root length, number of leaves and leaf area and four noded cuttings dipped in 800 ppm NAA, for 3-5 seconds was found to be the best successful propagule for passion fruit.

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Perbaikan Pembungaan Pamelo melalui Aplikasi Strangulasi dan Zat Pemecah Dormansi

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.7.3.139-145 ◽

2017 ◽

Vol 7 (3) ◽

pp. 139

Author(s):

Slamet Susanto ◽

Maya Melati ◽

Herik Sugeru

Keyword(s):

Carbohydrate Content ◽

Research Station ◽

Dormancy Breaking ◽

Randomized Design ◽

Breaking Dormancy ◽

Two Factors ◽

The Wire ◽

Research Experiment ◽

Agriculture Research Institute ◽

And Control

ABSTRACT Pummelo productivity is still low, therefore efforts should be made to increase its flowering and production. The objective of study was to determine the effectiveness of strangulation combined with the application of breaking dormancy substances to improve flowering of pummelo. The experiment was conducted in January to November 2015, at the Cikabayan Experimental Research Station, IPB. The carbohydrate and nitrogen analysis was done in laboratory of Postharvest Agriculture Research Institute, Bogor. Three-year-old pummelo grown in field was subjected for this research. Experiment used a completely randomized design (CRD) with two factors. The first factor was treatment to stimulate flowering, consisted of 3 levels i.e. single strangulation, double strangulation and control, and the second factor was the use of dormancy breaking substances, consisted of 3 types i.e. KNO3, Ethepon and BAP. Strangulation was performed by pressing the wire with a diameter of 2.0 mm into stem as deep as the diameter of the wire. Strangulation was done simultaneously and then released after 3 months. Dormancy breaking substance was applied immediately after releasing the wire for strangulation with the concentration of 200 ppm KNO3, 100 ppm Ethepon or 100 ppm BA. Treatments were replicated 4 times. The results showed that single and double strangulation treatments for 3 months were an effective way to induce flowering of young pummelo trees. Double strangulation produced more flowers as compared to single strangulation. Increased carbohydrate content and C/N ratio in leaves were observed on flower induced trees. Strangulation treatment for 3 months can increase flowering of young pummelo trees. Application of dormancy breaking substances did not have any effect on flowering induction in pummelo.Keywords: carbohydrate content, dormancy breaking substance, flower induction, pummelo, strangulationABSTRAK Produktivitas pamelo masih rendah sehingga perlu upaya peningkatan pembungaan dan produksinya. Penelitian ini bertujuan untuk mengetahui efektivitas strangulasi yang dikombinasikan dengan aplikasi zat pemecah dormansi dalam meningkatkan pembungaan jeruk pamelo. Penelitian dilaksanakan pada bulan Januari sampai Nopember 2015, bertempat di Kebun Percobaan IPB Cikabayan. Analisis karbohidrat dan nitrogen dilakukan di laboratorium BB Pasca Panen, Bogor. Percobaan menggunakan tanaman jeruk pamelo “Nambangan’ berumur 3 tahun dengan Rancangan Acak Lengkap (RAL) dua faktor. Sebagai faktor pertama yaitu perlakuan untuk menstimulasi pembungaan, terdiri atas 3 taraf perlakuan yaitu perlakuan strangulasi tunggal, strangulasi ganda dan kontrol, dan sebagai faktor kedua adalah penggunaan zat pemecah dormansi terdiri atas 3 jenis yaitu KNO3, Ethepon dan BAP. Masing-masing perlakuan diulang 4 kali sehingga terdapat 36 unit percobaan. Strangulasi dilaksanakan dengan melilitkan kawat dengan diameter 2.0 mm pada batang dengan menekan kawat ke batang sedalam diameter kawat tersebut. Strangulasi dilakukan serentak pada batang, strangulasi dilepas setelah 3 bulan kemudian. Zat pemecah dormansi diaplikasikan segera setelah pelepasan kawat strangulasi dengan konsentrasi masing-masing 200 ppm untuk KNO3, 100 ppm untuk Ethepon atau 100 ppm untuk BAP. Hasil penelitian menunjukkan perlakuan strangulasi selama 3 bulan merupakan cara efektif menginduksi pembungaan tanaman jeruk pamelo. Perlakuan strangulasi ganda menghasilkan bunga lebih banyak dibandingkan dengan strangulasi tunggal. Tanaman yang telah terinduksi menunjukkan tingginya kandungan karbohidrat pada tajuk tanaman sehingga meningkatkan rasio C/N. Perlakuan zat pemecah dormansi tidak memberikan pengaruh yang nyata terhadap pembungaan jeruk pamelo. Kata kunci: kandungan karbohidrat, pamelo, pembungaan, strangulasi, zat pemecah dormansi

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Growth Response of Mucuna Cuttings to Concentration and Duration of Soaking Shallot Extract

AGRINULA: Jurnal Agroteknologi dan Perkebunan ◽

10.36490/agri.v4i2.162 ◽

2021 ◽

Vol 4 (2) ◽

pp. 122-129

Author(s):

Enda Sabda Gentri Sembiring ◽

Julaili Irni ◽

Rama Riana Sitinjak ◽

Bayu Pratomo

Keyword(s):

Growth Response ◽

Block Design ◽

Duncan Multiple Range Test ◽

Soaking Time ◽

Extract Concentration ◽

Survival Percentage ◽

Randomized Block Design ◽

Two Factors ◽

Number Of Leaves ◽

Range Test

Introduction: This study aims to determine the growth response of Mucuna bracteata cuttings to the concentration and duration of soaking shallot extract. Materials and Methods: This study used a factorial Randomized Block Design (RBD) with two factors, namely: shallot extract concentration (P) consists of 4 levels, include P0= untreated, P1= 10 ml, P2= 20 ml, P3= 30 ml, meanwhile the soaking time (Q) consists of 4 levels, such as Q0= momentarily dipping, Q1= 15 minutes, Q2= 30 minutes, Q3= 45 minutes. The data was processed by ANOVA and if significant, then further tested with Duncan Multiple Range Test (DMRT) at the rate of 5%. Results: The concentration of shallot extract, soaking time and their interaction had a very significant effect on the survival percentage of Mucuna bracteata D.C. cuttings. The highest survival percentage of cuttings of Mucuna bracteata on the effect of shallot extract concentration and soaking time was obtained in the P0Q0, P0Q1, and P3Q2 treatments after the lid was opened at 4 weeks after planting. In the number of leaves also had significant effect in the interaction of two factors

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Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.6.1.20-28 ◽

2015 ◽

Vol 6 (1) ◽

pp. 20

Author(s):

Erna Sinaga ◽

Megayani Sri Rahayu ◽

Awang Maharijaya

Keyword(s):

Tissue Culture ◽

Drought Tolerance ◽

In Vitro Selection ◽

Solanum Melongena ◽

Survival Percentage ◽

Drought Tolerant ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design

ABSTRACTThe objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought tolerance of eggplant and the drought tolerant eggplant accessions. The experiment was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was arranged in a completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10, and 15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090). The results showed that the addition of PEG to in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought ABSTRAKPenelitian ini bertujuan untuk mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG) terhadap pertumbuhan tanaman terung in vitro, mendapatkan konsentrasi PEG yang dapat digunakan untuk seleksi tanaman terung secara in vitro dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor. Penelitian ini disusun dalam rancangan acak lengkap dua faktor. Faktor pertama adalah konsentrasi PEG terdiri atas 0, 5, 10, dan 15%. Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090). Hasil penelitian menunjukkan bahwa penambahan PEG pada media in vitro memberikan pengaruh nyata dan sangat nyata terhadap persentase hidup eksplan, persentase eksplan berkalus, pertambahan tinggi tunas, dan jumlah daun tanaman terung. Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan

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The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/905/1/012036 ◽

2021 ◽

Vol 905 (1) ◽

pp. 012036

Author(s):

Nandariyah ◽

L S Mahmudah ◽

R B Arniputri ◽

A T Sakya

Keyword(s):

Tissue Culture ◽

Coconut Water ◽

Emergence Time ◽

Shoot Height ◽

Culture Techniques ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Tissue Culture Techniques ◽

Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

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Response of Shallots of Batu Ijo Variety on Doses of N And K Fertilizers

Akta Agrosia ◽

10.31186/aa.24.1.19-24 ◽

2021 ◽

Vol 24 (1) ◽

pp. 19-24

Author(s):

Widodo Widodo ◽

Marlin Marlin ◽

Nancy B. Sitio

Keyword(s):

Plant Growth ◽

N Fertilizer ◽

Tuber Weight ◽

Plant Weight ◽

Randomized Design ◽

K Fertilizer ◽

Two Factors ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Higher Doses

Shallots (Allium cepa var. Aggregatum L) have many varieties that can be grown in the highlands and lowlands and one of them is the Batu Ijo variety. Nutrients N and K play an important role for plant needs. This research was conducted from March to June 2019 in Medan Baru, Bengkulu City. This study used a completely randomized design with two factors. The first factor was the dose of N fertilizer and the second factor was the application of K fertilizer. The application of N fertilizer had a significant effect on the number of shallots at week 2. The Urea dose given decreases the number of spring onions. For the best treatment at plant height is the treatment of Urea 300 kg/ha and KCl 50 kg/ha. Plant growth from week 2 to 5 increased significantly. The application of K fertilizer has an effect on the number of shallots at week 2 and the number of tubers. The higher the plant age, the more the number of leaves produced, but at higher doses, the number of leaves and the number of tubers decreased. The best treatment for the number of leaves produced was Urea 300 kg/ha and without giving KCl. For the best treatment of fresh plant weight, namely Urea 300 kg/ha and KCl 150 kg/ha, the best treatment fresh tuber weight was Urea 300 kg/ha and KCl 150 kg/ha, for tuber diameter the best treatment was 200 kg/ha of urea and KCl 100 kg/ha and for the number of tubers of Urea 0 kg/ha and KCl 150 kg/ha.Keywords: shallots, N fertilizer, K fertilizer

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ALTERNATIVE SUBSTRATES FOR PRODUCTION OF YELLOW PASSION FRUIT SEEDLINGS

JOURNAL OF NEOTROPICAL AGRICULTURE ◽

10.32404/rean.v7i1.3890 ◽

2020 ◽

Vol 7 (1) ◽

pp. 43-48

Author(s):

Eduardo Santana Aires ◽

Carlos Alberto Aragão ◽

Itala Laiane Silva Gomes ◽

Gilmário Noberto de Souza ◽

Isa Gabriela Vieira de Andrade

Keyword(s):

Plant Height ◽

Dry Matter ◽

Passion Fruit ◽

Dry Matter Accumulation ◽

Alternative Substrates ◽

Randomized Design ◽

Length Width ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Yellow Passion Fruit

This study aimed to evaluate different substrates to produce yellow passion fruit seedlings. For this purpose, an experiment was carried out in a greenhouse, from April to June 2016. A completely randomized design with five replications, in a 6 x 5 factorial scheme was used. The first factor was the alternative substrates (poultry, cattle and equine manure, sugarcane and coconut bagasse, and Organomais compost). The second factor was the replacement levels to the commercial substrate of Plantmax® (100:0, 75:25, 50:50, 25:75, 0:100). The seedling evaluation was carried out 42 days after sowing. The following variables were analyzed: length, width, and the number of leaves; plant height and shoot dry matter. A significant effect (P0.05) for the interaction between the factors was verified. The addition of 60% cattle manure and Organomais to the commercial substrate Plantmax® promoted greater length, width, and the number of leaves, plant height, and shoot dry matter accumulation in the yellow passion fruit seedlings.

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ADDITION OF THIAMIN AND LEAF FOLIAR IN ABACA BANANA (Musa textillis Nee.) ACCLIMATIZATION

Agrivet ◽

10.31315/agrivet.v25i2.4286 ◽

2021 ◽

Vol 25 (2) ◽

pp. 88

Author(s):

Rina Srilestari ◽

Suwardi Suwardi

Keyword(s):

Raw Materials ◽

Paper Industry ◽

Economic Value ◽

Large Space ◽

Planting Material ◽

Randomized Design ◽

Number Of Leaves ◽

The Right ◽

Leaf Fertilizer ◽

Range Test

The economic value of the Abaca banana is on the trunk which contains fiber for valuable textile and paper industry raw materials. The multipurpose fiber and its prospects are quite good, so the abaca plant gets a lot of attention from various community groups both private, state-owned, cooperative and farmers. Propagation through multiplication humps is very low, planting material is easily damaged in transportation, is not durable and requires large space so that transportation costs are high. One alternative to overcome the provision of healthy, large and fast seedlings is to use seedlings from tissue culture. The aim of the study is to get the right thiamin and leaf fertilizer concentrations at the acclimatization stage. The experimental design used was a 2-factor Complete Randomized Design, Thiamin concentration (1 ml / l, 2 ml / l, 3 ml / l) and leaf fertilizer (0.5 ml / l, 1 ml / l, 1.5 ml / l). The data obtained were analyzed for diversity at 5% level and further tests using Duncan Multiple Range Test (DMRT) level of 5%. The results showed no interaction between thiamin and leaf fertilizer, Thiamin 2 ml / l gave the best results on all parameters and 1.5 ml / l leaf fertilizer could increase plant height and the number of leaves of abaca banana plants.Keywords: Thiamin, leaf fertilizer, abaca banana, acclimatization

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Effect of Potting Mixture on Growth of Commiphora wightii (Arn.) Bhandari Through Semi-Hardwood Cuttings

Journal of Non Timber Forest Products ◽

10.54207/bsmps2000-2017-512mnu ◽

2017 ◽

Vol 24 (4) ◽

pp. 239-242

Author(s):

A. Sure ◽

S. Khachane ◽

A. Nimkar

Keyword(s):

Medicinal Plant ◽

Chlorophyll Content ◽

Low Cost ◽

Commiphora Wightii ◽

Experimental Trial ◽

Planting Material ◽

Randomized Design ◽

Endangered Medicinal Plant ◽

Number Of Leaves ◽

Completely Randomized Design

An experimental trial was conducted to find out the effective potting mixture and low cost quality planting material through semi-hardwood cuttings propagation of endangered medicinal plant Commiphora wightii (Arn.) Bhandari. The experiment was carried out in CRD (Completely Randomized Design) with six treatments, three replications and 20 cuttings per treatment. Growth in cutting was measured by eleven parameters. Parameter such as sprouting percentage (88.67%), rooting percentage (83.45%), minimum days for sprouting (9.82), number of sprouts (7.17), length of shoot (15.25cm), diameter of shoot (3.950mm), number of leaves (24.83), chlorophyll content a, b and total (7.6290, 5.7083 and 13.3374 mg/100g, respectively) was significantly superior under potting mixture S+S+V (soil+sand+vermicompost). Furthermore rooting parameter such as number of roots (7.51), length of root (14.60cm), diameter of root (1.338mm) was found higher in media containing S+S+C (soil+sand+cocopeat).

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Sugar Cane Cake and Mineral Fertilizers on Coconut (Cocos nucifera Linn.) Seedlings

CORD ◽

10.37833/cord.v25i2.142 ◽

2009 ◽

Vol 25 (2) ◽

pp. 11

Author(s):

Luiz Gonzaga Bione Ferraz ◽

Ademar Barros da Silva ◽

José Nunes Filho ◽

Antonio Raimundo de Sousa ◽

Venézio Felipe dos Santos

Keyword(s):

Sugar Cane ◽

Chemical Elements ◽

Cocos Nucifera ◽

Block Design ◽

Organic Fertilizer ◽

Mineral Fertilizers ◽

Research Station ◽

Linear Quadratic ◽

Two Factors ◽

And Control

High quantities of mineral fertilizers (MF) are required for good coconut seedling production, whose up dated recommendation is 232 grams per seedling. The objective of this work was to assess the substitution of the MF by organic fertilizer as sugar-cane filter cake (SCFC) on coconut seedlings in polybag. The SCFC is an organic residue from sugar-cane manufacturing composed of various chemical elements, as for instance: nitrogen, phosphorus, potassium, calcium and magnesium. The experiment was carried out at the Itapirema Research Station, Goiana, Pernambuco State, Brazil, in a randomized complete block design, with factorial disposition 52, replicated three times, with six tall coconut seedlings per plot. The treatments were the combinations of five doses of MF (in g seedling-1): 46.25; 92.50; 138.75; 185.00 and control, using urea, simple superphosphate and potassium chloride at the proportion of 1:2:3, respectively, and five doses of SCFC (in kg polybag-1): 0.5; 1.0; 1.5; 2.0 and control. Eight months after young coconut seedlings replacement to the polybags the following parameters were measured: collar girth (CG), leaf area (LA), number of live leaves (NL) and plant height (PH). For leaf nutritional diagnosis and growing media chemical and physical analyses, samples were collected. There was significance for the two factors, individually, to all parameters. The trend analysis revealed linear, quadratic and cubic responses, according to the studied character. The mixture of 0.5 kg of SCFC polybag-1 plus 46.25 g of the MF was sufficient for obtaining seedlings of excellent quality and promoting a fertilizers economy around 80%. According to other results from this research, the number of seedlings per plot and the critical level of nutrients in the leaf of coconut seedlings should be reviewed.

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PENGARUH NAA DAN BAP TERHADAP PERTUMBUHAN SUBKULTUR ANGGREK HASIL PERSILANGAN Dendrobium biggibum X Dendrobium liniale

Caraka Tani Journal of Sustainable Agriculture ◽

10.20961/carakatani.v31i1.11938 ◽

2016 ◽

Vol 31 (1) ◽

pp. 33

Author(s):

Sri Hartati ◽

Agus Budiyono ◽

Ongko Cahyono

Keyword(s):

Plant Conservation ◽

Naphthalene Acetic Acid ◽

Randomized Design ◽

Significant Difference ◽

Two Factors ◽

Number Of Leaves ◽

Media Research ◽

Test Level ◽

Range Test

Indonesia has a high diversity of species of orchids. One of them is the Dendrobium orchid. Orchid seeds do not have endosperm should be grown in a medium that has enough nutrients. Orchid propagation in vitro is strongly influenced by the composition of the medium used. Research through experiments aimed at studying the effect of NAA, BAP, NAA and BAP combination of the subculture plantlets growth of hybrids orchid Dendrobium biggibum X liniale in Vacin Went media. Research conducted at the Laboratory of Tissue Culture Center for Plant Conservation Bogor Botanical Gardens. The experimental design used was completely randomized design (CRD) with two factors. The first factor was the concentration of Naphthalene Acetic Acid /NAA ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). The second factor was the type media of Benzyl Amino Purine /BAP ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). Each treatment was replicated eight times. Analysis of the data by F test level 5% and if there is a significant difference continued with Duncan Multiple (Duncan's Multiple Range Test) level of 5%. The result showed that the addition of NAA 3 ppm as much as 4,96 cm and BAP 3 ppm as much as 4,41 cm give a significant effect on the increase of high plantlets and so NAA 3 ppm as much as 5,76 cm effect on roots length , but did not significantly effect the number of leaves and roots.

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