scholarly journals Antibiogram Testing of Bacterial Isolates from Cassava, Yam and Plantain Flours and Shelf Life Studies of the Products Sold in Some Markets in Port Harcourt, Nigeria

2019 ◽  
pp. 1-11
Author(s):  
M. Elenwo ◽  
N. Maduka ◽  
N. N. Odu
Keyword(s):  
Moisture Content ◽  
Shelf Life ◽  
Nalidixic Acid ◽  
Viable Count ◽  
Total Viable Count ◽  
Bacterial Isolates ◽  
Gram Positive ◽  
Gram Negative ◽  
Yam Flour ◽  
Shelf Life Studies

Bacterial resistance to commonly used antibiotics is a threat to public health. This study focused on antibiogram testing of bacterial isolates from packaged and exposed cassava, plantain and yam flour using chloramphenicol, ciprofloxacin, erythromycin, levofloxacin, gentamicin, ampiclox, rifampicin, amoxil, streptomycin, norfloxacin, ampicillin, ceporex, tarivid, nalidixic acid, peflacine, augmentin and septrin. Shelf life studies involved monitoring total viable count, total fungal count and moisture content of the flour samples stored at room temperature (28± 2°C) at 1 Wk interval for 4 Wks. Standard methods and agar diffusion technique were adopted. Bacillus sp. and Staphylococcus sp. (Gram-positive) were identified in all the flour samples. Salmonella sp. and Escherichia coli (Gram negative) were isolated from exposed plantain and yam flour, respectively. Gram-positive isolates susceptible and resistant to each antibiotic ranged between 65.38-96.15% and 3.85-30.77%, respectively whereas that of Gram-negative isolates was between 25-75%. Antibiogram testing revealed that 76.92 and 30.77% of Bacillus and Staphylococci isolates, respectively were sensitive; 100% Salmonella sp. and E. coli (isolate OMY) was resistant and sensitive, respectively. Among antibiotics used for Gram-positive bacteria, rifampicin was most effective whereas streptomycin, tarivid, nalidixic acid, gentamycin, augmentin and ciprofloxacin were more effective than other antibiotics used for Gram negative bacteria. During storage of the flour samples, moisture content (8.6-23.20%) and total viable count (6.47-6.86 log10cfu/g) increased but total fungal count (3.53-2.15 log10cfu/g) decreased with few exceptions. Therefore, reduction in microbial contamination of edible flours by implementing good manufacturing practices and proper packaging of the commercialized products could reduce the spread of antimicrobial resistant bacteria.

scholarly journals Bacteriological Quality Assessment of Raw Beef Sold in Sylhet Sadar

2016 ◽  
Vol 13 (2) ◽  
pp. 9-16 ◽  
Author(s):  
Farhana Jahan ◽  
A T M Mahbub-E-Elahi ◽  
A B Siddique
Keyword(s):  
Viable Count ◽  
Total Viable Count ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Fresh Meat ◽  
Gram Negative ◽  
Bacteriological Quality ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

This study was conducted to assess the bacteriological quality of fresh raw beef sold in different markets of Sylhet Sadar. A total of seventy five (75) fresh raw beef samples were randomly collected from seven  major markets (Shibgonj, Mirabazar, Kazitula, Ambarkhana, Madina market, Bandar bazar and Sheikh ghat) of Sylhet Sadar. Total Viable Count of the samples ranged between 2.5 × 105 to 2.25 × 108cfu/g. Acceptability for consumption of these samples as satisfactory, acceptable and rejected were 40, 32 and 28%, respectively. A total of 115 bacterial isolates of 5 genera were identified including gram negative Escherichia coli [15(10%)], Salmonella spp. [20(13.33%)], Klebsiella spp. [30(20%)], Enterobacter spp. [10(6.67%)] and gram positive Staphylococcus spp. [40(26.67%)]. The presence of these organisms in fresh meat from conventional beef is alarming.DOI: http://dx.doi.org/10.3329/agric.v13i2.26654The Agriculturists 2015; 13(2) 09-16

scholarly journals Bacterial Isolates and its Antibiotic Susceptibility Pattern in NICU

2014 ◽  
Vol 11 (1) ◽  
pp. 66-70 ◽  
Author(s):  
S Shrestha ◽  
NC Shrestha ◽  
S Dongol Singh ◽  
RPB Shrestha ◽  
S Kayestha ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Neonatal Intensive Care ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Gram Positive ◽  
Antibiotic Susceptibility Pattern ◽  
Gram Negative ◽  
Nosocomial Sepsis

Background Neonatal sepsis is one of the major causes of morbidity and mortality among the newborns in the developing world. Objectives To determine the common bacterial isolates causing sepsis in neonatal intensive care unit and its antibiotic susceptibility pattern. Methods A one year discriptive prospective study was conducted in neonatal intensive care unit to analyse the results of blood culture and to look into the sensitivity of the commonly used antibiotics. Results The blood culture yield by conventional method was 44.13% with nosocomial sepsis accounting for 10.79%. 84.08% were culture proven early onset sepsis and 15.95% were late onset sepsis. Klebsiella infection was the commonest organism isolated in early, late and nosocomial sepsis but statistically not significant. Gram positive organisms were 39.36% in which Staphylococcus aureus was the leading microorganism followed by coagulase negative staphylococcus areus. Gram negative organisms were 60.64% amongst them Klebsiella was the most often encountered followed by Pseudomonas. The most common organism Klebsiella was 87.5% and 78.3% resistance to ampicillin and gentamycin respectively. Among gram negative isolates 87.5% and 77.2% were resistance to ampicillin and gentamycin respectively. Among gram positive isolates 58.5% and 31.5% resistance were noted to ampicillin and gentamycin respectively. Resistance to cefotaxim to gram negative and gram positive isolates were 87.34% and 59.35% respectively. Conclusion Klebsiella is most common organism which is almost resistance to first line antibiotics. Resistance to both gram negative and gram positive isolates among firstline antibiotics and even with cefotaxim is emerging and is a major concern in neonatal intensive care unit. DOI: http://dx.doi.org/10.3126/kumj.v11i1.11030 Kathmandu University Medical Journal Vol.11(1) 2013: 66-70

scholarly journals Microbiological Screening and Antimicrobial Sensitivity Profiling of Wound Infections in a Tertiary Care Hospital of Bangladesh

2020 ◽  
pp. 101-106
Keyword(s):  
Wound Infection ◽  
Primary Care Physicians ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Treatment Modalities ◽  
Care Hospital ◽  
Bacterial Isolates ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Sensitivity

Wound infection is a major problem in hospitals in developing countries. Wound infection causes morbidity and prolonged hospital stay thus this prospective study was conducted for a period of seven months (January 2019 to July 2019). A total of 217 specimens (wound swabs and pus exudates) from wound infected patients in a Tertiary Care Hospital in Bangladesh. A retrospective study of the microbiological evaluation was done by cultural growth as well as Gram staining and biochemical examination to identify the bacterial isolates. Finally, the antimicrobial vulnerability testing was performed by Kirby-Bauer disc diffusion conventional method. A total of 295 samples were tested. Out of which 217 (73.5%) were found culture positive. E. coli was the most predominant gram-negative isolates whereas Staphylococcus aureus and Coagulase-negative Staphylococcus were the most commonly isolated gram-positive organisms. Antimicrobial sensitivity profile of bacterial isolates revealed imipenem, meropenem, amikacin, and nitrofurantoin to be the most effective antimicrobials against gram-negative isolates, whereas imipenem, meropenem, amikacin, nitrofurantoin, amoxiclav, and gentamicin were the most effective drugs against gram-positive isolates. The result of this examination contributes to the identification of basic causative microbes involved in wound infection and findings of antibiotic susceptibility patterns can be helpful for primary care physicians to optimize the treatment modalities, articulate policies for empiric antimicrobial therapy, and to minimize the rate of infection among wound infected patients.

scholarly journals Diversity and Antibiotic Susceptibility of Bacteria in Water of Hotel Restaurants in Dhaka City

2017 ◽  
Vol 43 (2) ◽  
pp. 173-180
Author(s):  
Md Abdul Karim ◽  
Nasrin Sultana
Keyword(s):  
Bacterial Abundance ◽  
Bacterial Count ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Genus Bacillus ◽  
Gram Positive ◽  
Gram Negative ◽  
Agar Media ◽  
Micrococcus Roseus ◽  
Microbiological Status

Present study was conducted to determine the microbiological status of water from dispensers in different roadside hotel and restaurants of Dhaka city. Samples were collected from seven hotel and restaurants. Aerobic heterotrophic bacterial count ranged between 1.5 × 10 and 8.8 × 103 cfu/ml. Enteric and related bacterial abundance in MacConkey, SS and Cetrimide agar media ranged from 0 to 4.9 × 106, 0 to 2.1 × 105 and 0 to 1.2 × 106cfu/ml, respectively. In total, 28 bacterial isolates were obtained during the study period. Among them, 15 were heterotrophic isolates and 13 were enteric and related bacteria. Among 15 aerobic heterotrophic isolates, 11 were gram positive and five were gram negative. Out of 11 gram positive isolates, 7 belonged to the genus Bacillus viz. B. circulans, B. subtilis, B. stearothermophilus, B. brevis and B. cereus and one to coccus viz. Micrococcus roseus. The other gram positive species were Kurtia gibsonii, Auriobacterium liguefaciens and Curtobacterium luteum. Four gram negative isolates were Neisseria elongate sub. spp. glycolytica, Plesiomonas shigelloides, Pseudomonas fluorescens biovar 1, Pseudomonas aeruginosa. All 13 enteric and related isolates were gram negative, short rod; and non-spore formers and belonged to the genera Escherichia, Klebsiella, Shigella and Pseudomonas. Among all isolates, two were resistant and six were susceptible to all five antibiotics. Asiat. Soc. Bangladesh, Sci. 43(2): 173-180, December 2017

scholarly journals Modeling the Effect of Active Modified Atmosphere Packaging on the Microbial Stability and Shelf Life of Gutted Sea Bass

2019 ◽  
Vol 9 (23) ◽  
pp. 5019 ◽  
Author(s):  
Theofania Tsironi ◽  
Athina Ntzimani ◽  
Eleni Gogou ◽  
Maria Tsevdou ◽  
Ioanna Semenoglou ◽  
...  
Keyword(s):  
Growth Rate ◽  
Shelf Life ◽  
Microbial Growth ◽  
Modified Atmosphere Packaging ◽  
Sea Bass ◽  
Viable Count ◽  
Lag Phase ◽  
Modified Atmosphere ◽  
Total Viable Count ◽  
Microbial Stability

The aim of the study was the evaluation and mathematical modeling of the effect of active modified atmosphere packaging (MAP), by the incorporation of CO2 emitters in the package, on the microbial stability and shelf life of gutted sea bass during refrigerated storage. Gutted sea bass samples were packaged in modified atmosphere (50% CO2–40% N2–10% O2) with and without CO2 emitters (ACT-MAP, MAP) (gas/product volume ratio 3:1) and stored at isothermal conditions: 0 °C, 5 °C, and 10 °C. The gas concentration in the package headspace (%CO2, %O2) and microbial growth (total viable count, TVC, Pseudomonas spp., Enterobacteriaceae spp., lactic acid bacteria) were monitored during storage. The microbial growth was modeled using the Baranyi growth model, and the kinetic parameters (microbial growth rate, lag phase) were estimated at the tested temperature and packaging conditions. The results showed that the ACT-MAP samples presented significantly lower microbial growth compared to the MAP samples. The growth rate of the total viable count at 0 °C was 0.175 and 0.138 d−1 for the MAP and ACT-MAP sea bass, respectively (p < 0.05). The shelf life of the MAP sea bass at 0–10 °C (based on a final TVC value: 7 log CFU g−1) was extended 4–7 days with the addition of a CO2 emitter in the package. The CO2 concentration in the ACT-MAP samples was stabilized at approximately 60%, while the CO2 in the MAP samples was approximately 40% at the end of the shelf life.

scholarly journals Antimicrobial Resistance Patterns of Bacterial Isolates from Blood Culture among HIV/AIDS Patients at Felege Hiwot Referral Hospital, Northwest Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Mohabaw Jemal ◽  
Teshiwal Deress ◽  
Teshome Belachew ◽  
Yesuf Adem
Keyword(s):  
Antimicrobial Resistance ◽  
Blood Culture ◽  
Agar Plate ◽  
Blood Cultures ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Aids Patients ◽  
Gram Positive ◽  
Gram Negative ◽  
Hiv Aids

Background. The emergence and spread of antimicrobial resistance in bacteria is recognized as a global public health problem. Bloodstream infection with antimicrobial-resistant bacteria in HIV/AIDS patients makes the problem more challenging. So, regular and periodic diagnosis and use of the appropriate antimicrobial susceptibility pattern determination is the only option for decreasing the prevalence and development of drug-resistant bacteria. Methods. An institution-based cross-sectional study was conducted among 384 HIV/AIDS patients. Sociodemographic data of patients were recorded using structured questionnaires. Blood cultures were collected with BACTEC aerobic blood culture bottles. A pair of samples was collected from each patient aseptically and incubated at 37°. If samples are positive for bacterial agents, they were subcultured to solid media such as blood agar plate, chocolate agar plate, and MacConkey agar plates. Identification was performed using colony characteristics and standard biochemical techniques. The antimicrobial susceptibility test was determined by the Kirby–Bauer disc diffusion method. Data entry and analysis were performed while using SPSS version 20. Descriptive statistics were performed to calculate frequencies. Results. Altogether, 384 patients were included, and 123 blood cultures were positive, so that the yield was thus 32%. About 46 (37.4%) of Gram-negative and 77 (62.6%) of Gram-positive bacterial species were identified. Among Gram-negative bacterial isolates, K. pneumoniae was the leading pathogen, 19 (41.3%), whereas S. aureus, 38 (49.4%), was predominant among Gram-positive isolates. In his study, the majority of Gram-positive isolates showed high level of resistance to penicillin, 72 (95.5%), tetracycline, 55 (71.4%), and cotrimoxazole, 45 (58.4%). About 28 (73.6%) of S. aureus isolates were also methicillin-resistant. Gram-negative bacterial isolates also showed a high resistance to ampicillin (91.3%), tetracycline (91.3%), and gentamicin (47.8%). Overall, about 78% of multidrug resistance was observed. Conclusion. Several pathogens were resistant to greater than five antimicrobial agents, so that proper management of patients with bacteremia is needed, and a careful selection of effective antibiotics should be practiced.

SHELF LIFE AND CULINARY PROPERTIES OF THAWED FROZEN PASTEURIZED WHOLE EGG

1969 ◽  
Vol 32 (9) ◽  
pp. 362-364 ◽  
Author(s):  
D. V. Vadehra ◽  
F. R. Steele ◽  
R. C. Baker
Keyword(s):  
Shelf Life ◽  
Viable Count ◽  
Total Viable Count ◽  
Whole Egg

Commercially pasteurized whole egg had a shelf life (total viable count slightly in excess of 106 bacteria per gram) of approximately 15, 9, 2, and 1 days at 2, 7, 13, and 23 C, respectively. The thawed frozen pasteurized product has a maximum shelf life of 9 days at 2 C and 3 days at 13 C. The thawing temperature of the frozen product did not influence its shelf life to any great extent. Culinary properties of the fresh, pasteurized and thawed frozen pasteurized egg were very similar.

Direct viable count of Gram-positive and Gram-negative bacteria using ciprofloxacin as inhibitor of cellular division

1995 ◽  
Vol 22 (2) ◽  
pp. 139-150 ◽  
Author(s):  
Isabel Barcina ◽  
Inés Arana ◽  
Patricia Santorum ◽  
Juan Iriberri ◽  
Luis Egea
Keyword(s):  
Viable Count ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Cellular Division ◽  
Gram Negative

scholarly journals The Effect of Packaging Methods on the Shelf-life of Iron Fortified Mozzarella Cheese

2021 ◽  
Author(s):  
M. Raquib ◽  
T. Borpuzari ◽  
M. Hazarika ◽  
S.K. Laskar ◽  
G.K. Saikia ◽  
...  
Keyword(s):  
Shelf Life ◽  
Goat Milk ◽  
Storage Period ◽  
Viable Count ◽  
Vacuum Packaging ◽  
Cow Milk ◽  
Total Viable Count ◽  
Mozzarella Cheese ◽  
Kiwi Fruit ◽  
Treatment Groups

Background: The present study was undertaken to study the effect of different packaging methods on the shelf-life of iron fortified mozzarella cheese prepared from cow milk, goat milk and mixed milk using kiwi fruit extract.Methods: Mozzarella cheese samples were packed both aerobically and under vacuum packaging condition and microbiological load (Total viable count, Escherichia coli, Salmonella, Shigella, Staphylococcus aureus, yeast and moulds and spores count) were monitored throughout the storage period in order to determine its shelf-life. Result: The total viable count (TVC) increased gradually from 0 day till 15 days of refrigerated storage for all the samples of iron fortified mozzarella cheese under both aerobic and vacuum packaging conditions irrespective of the types of milk and enzymes used. Under aerobic packaging condition, highest total viable count was observed for goat’s milk sample in both control and treatment groups all throughout the storage period. Mixed milk iron fortified mozzarella cheese exhibited least count in both the groups. The TVC of all the cheese samples were higher in aerobic packaging compared to vacuum packaging condition. The shelf-life or best before use of the product could judged as 15d from the date of manufacturing.

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