scholarly journals Diversity and Antibiotic Susceptibility of Bacteria in Water of Hotel Restaurants in Dhaka City

2017 ◽  
Vol 43 (2) ◽  
pp. 173-180
Author(s):  
Md Abdul Karim ◽  
Nasrin Sultana
Keyword(s):  
Bacterial Abundance ◽  
Bacterial Count ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Genus Bacillus ◽  
Gram Positive ◽  
Gram Negative ◽  
Agar Media ◽  
Micrococcus Roseus ◽  
Microbiological Status

Present study was conducted to determine the microbiological status of water from dispensers in different roadside hotel and restaurants of Dhaka city. Samples were collected from seven hotel and restaurants. Aerobic heterotrophic bacterial count ranged between 1.5 × 10 and 8.8 × 103 cfu/ml. Enteric and related bacterial abundance in MacConkey, SS and Cetrimide agar media ranged from 0 to 4.9 × 106, 0 to 2.1 × 105 and 0 to 1.2 × 106cfu/ml, respectively. In total, 28 bacterial isolates were obtained during the study period. Among them, 15 were heterotrophic isolates and 13 were enteric and related bacteria. Among 15 aerobic heterotrophic isolates, 11 were gram positive and five were gram negative. Out of 11 gram positive isolates, 7 belonged to the genus Bacillus viz. B. circulans, B. subtilis, B. stearothermophilus, B. brevis and B. cereus and one to coccus viz. Micrococcus roseus. The other gram positive species were Kurtia gibsonii, Auriobacterium liguefaciens and Curtobacterium luteum. Four gram negative isolates were Neisseria elongate sub. spp. glycolytica, Plesiomonas shigelloides, Pseudomonas fluorescens biovar 1, Pseudomonas aeruginosa. All 13 enteric and related isolates were gram negative, short rod; and non-spore formers and belonged to the genera Escherichia, Klebsiella, Shigella and Pseudomonas. Among all isolates, two were resistant and six were susceptible to all five antibiotics. Asiat. Soc. Bangladesh, Sci. 43(2): 173-180, December 2017

scholarly journals Bacterial status and pollution level in the water of Gulshan Lake, Dhaka

2013 ◽  
Vol 40 (2) ◽  
pp. 165-173 ◽  
Author(s):  
Md Abdul Karim ◽  
Nazneen Akhter ◽  
Sirajul Hoque
Keyword(s):  
Lake Water ◽  
Bacterial Abundance ◽  
Point Of View ◽  
Pollution Level ◽  
Bacterial Isolates ◽  
Macconkey Agar ◽  
Gram Positive ◽  
Gram Negative ◽  
Different Seasons ◽  
Agar Media

Water samples were collected from four selected sites of the Gulshan lake during four different seasons. Four different media were used to study the aerobic heterotrophic, enteric and related bacterial abundance. Aerobic heterotrophic bacterial abundance ranged between 1×106 and 9×106 cfu/100 ml, while enteric and related bacterial abundance in SS agar, MacConkey agar and Cetrimide agar media ranged between 1×103 and 3×105 cfu/100 ml, between 1×104 and 1×107, and between 1×100 and 1.4×103 cfu/100 ml, respectively. Among 40 bacterial isolates 14 were selected out of which seven were Gram-positive and seven were Gramnegative. Among Gram-positive isolates, six were rod-shaped spore former belonging to Bacillus and Lactobacillus, while another one was coccoid in shape and under the genus Planococcus. Out of seven Gram-negative isolates, two were the members of Pseudomonas, remaining five were Morganella, Neisseria, Escherichia, Aeromonas and Enterobacter. Inorganic nutrients like NH4+-N, NO3+-N, NO2+-N and PO4+ values were found to be satisfactory from pollution point of view. The presence of different bacterial isolates indicate that the Gulshan lake water was polluted with chemical and bacterial pollutants. DOI: http://dx.doi.org/10.3329/bjz.v40i2.14309 Bangladesh J. Zool. 40(2): 165-173, 2012

scholarly journals Bacterial status and pollution level in the sediment of Gulshan Lake, Dhaka

2013 ◽  
Vol 38 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Md Abdul Karim ◽  
Nazneen Akhter ◽  
Sirajul Hoque
Keyword(s):  
Bacterial Count ◽  
Pollution Level ◽  
Bacterial Isolates ◽  
Residential Areas ◽  
Bacterial Strains ◽  
Genus Bacillus ◽  
Nitrogen And Phosphorus ◽  
Gram Positive ◽  
Gram Negative ◽  
Different Seasons

Gulshan-Baridhara lake located in and around Gulshan, Banani and Baridhara, has always been Dhaka’s most exclusive residential area mainly because of the pristine lake. The lake is elongated in a north south direction and surrounded mainly by residential areas and some industrial units as well. To evaluate the pollution level of the sediment of the lake, samples were collected from 4 selected locations in 4 different seasons. Four different media were used to study the aerobic heterotrophic, enteric and related bacterial count. Aerobic heterotrophic bacterial count was in the range of 1.1 × 104 to 1.2 ×106 cfu/g in sediment sample, while enteric and related bacterial count in 3 different media (SS agar, MacConkey agar and Cetrimide agar) were 1×100 to 1.5×104 cfu/g, 1×100 to 6×104, and 1×100 to 1.1×103 cfu/g in sediment, respectively. Out of 23 bacterial isolates 18 were Gram-positive and 5 were Gram-negative. Among the Gram-positive, all were rod shaped spore former and under the genus Bacillus. Out of 5 Gram-negative isolates 2 were the member of Pseudomonas, remaining 3 strains were Escherichia, Aeromonas and Enterobacter. Total nitrogen and phosphorus level along with the presence of different bacterial strains clearly indicated that Gulshan lake sediment was polluted with the chemical and bacterial pollutants. DOI: http://dx.doi.org/10.3329/jasbs.v38i2.15596 J. Asiat. Soc. Bangladesh, Sci. 38(2): 155-161, December 2012

scholarly journals Isolation and Characterization of Bacteria with Biochemical and Pharmacological Importance from Soil Samples of Dhaka City

2017 ◽  
Vol 16 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Kohinur Begum ◽  
Sultana Juhara Mannan ◽  
Refaya Rezwan ◽  
Md Mahinur Rahman ◽  
Md Shajidur Rahman ◽  
...  
Keyword(s):  
Bioactive Compound ◽  
Soil Samples ◽  
Penicillin G ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Gram Positive ◽  
Gram Negative ◽  
Isolation And Characterization

We studied soil samples from Dhaka municipality area to isolate and characterize bacteria having potential biochemical and pharmacological importance. Total twenty five soil samples were collected from fish, vegetables and fruits dump area from Dhaka City. Bacterial population was sub-cultured in trypticase soya agar (TSA) plate. Nineteen colonies were isolated, cultured and characterized by gram staining and biochemical tests. Six isolates were found to be gram negative while thirteen were gram positive. All isolates were positive in oxidase, catalase, citrate, and protease tests. Eight isolates showed coagulase negative and nine were coagulase positive. It was found that all bacterial isolates were sensitive to tetracycline, chloramphenicol, gentamycin, ciprofloxacin, azithromycin and ceftriaxone. About 95% of the bacterial isolates were resistant to penicillin-G and ampicillin. About 89%, 26%, 21% and 11% of the bacterial isolates were resistant to amoxicillin, co-trimoxazole, nalidixic acid and erythromycin, respectively. It was found that bacterial isolates produce chemical(s) inhibitory to other bacterial strains including both gram positive and gram negative bacteria. Further studies are needed to characterize the potential antibacterial factor(s) and other bioactive compound (s) present in these bacterial isolates from soil samples.Dhaka Univ. J. Pharm. Sci. 16(1): 129-136, 2017 (June)

scholarly journals Bacterial Isolates and its Antibiotic Susceptibility Pattern in NICU

2014 ◽  
Vol 11 (1) ◽  
pp. 66-70 ◽  
Author(s):  
S Shrestha ◽  
NC Shrestha ◽  
S Dongol Singh ◽  
RPB Shrestha ◽  
S Kayestha ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Neonatal Intensive Care ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Gram Positive ◽  
Antibiotic Susceptibility Pattern ◽  
Gram Negative ◽  
Nosocomial Sepsis

Background Neonatal sepsis is one of the major causes of morbidity and mortality among the newborns in the developing world. Objectives To determine the common bacterial isolates causing sepsis in neonatal intensive care unit and its antibiotic susceptibility pattern. Methods A one year discriptive prospective study was conducted in neonatal intensive care unit to analyse the results of blood culture and to look into the sensitivity of the commonly used antibiotics. Results The blood culture yield by conventional method was 44.13% with nosocomial sepsis accounting for 10.79%. 84.08% were culture proven early onset sepsis and 15.95% were late onset sepsis. Klebsiella infection was the commonest organism isolated in early, late and nosocomial sepsis but statistically not significant. Gram positive organisms were 39.36% in which Staphylococcus aureus was the leading microorganism followed by coagulase negative staphylococcus areus. Gram negative organisms were 60.64% amongst them Klebsiella was the most often encountered followed by Pseudomonas. The most common organism Klebsiella was 87.5% and 78.3% resistance to ampicillin and gentamycin respectively. Among gram negative isolates 87.5% and 77.2% were resistance to ampicillin and gentamycin respectively. Among gram positive isolates 58.5% and 31.5% resistance were noted to ampicillin and gentamycin respectively. Resistance to cefotaxim to gram negative and gram positive isolates were 87.34% and 59.35% respectively. Conclusion Klebsiella is most common organism which is almost resistance to first line antibiotics. Resistance to both gram negative and gram positive isolates among firstline antibiotics and even with cefotaxim is emerging and is a major concern in neonatal intensive care unit. DOI: http://dx.doi.org/10.3126/kumj.v11i1.11030 Kathmandu University Medical Journal Vol.11(1) 2013: 66-70

scholarly journals Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Kesi Kurnia ◽  
Nina Hermayani Sadi ◽  
Syafitri Jumianto
Keyword(s):  
Heterotrophic Bacteria ◽  
Water Environment ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Gram Negative ◽  
Isolation And Characterization ◽  
Agar Media ◽  
Physical And Chemical ◽  
Standard Disk

<span>Pollution of water environment with heavy metals is becoming one of the most severe environmental and human health hazards. Lead (Pb) is a major pollutant and highly toxic to human, animals, plants, and microbes. </span><span lang="IN">Toxic metals are difficult to remove from the environment, since they cannot be chemically or biologically degraded and are ultimately indestructible. Biological approaches based on metal-resistant microorganisms have received a great deal of attention as alternative remediation processes. </span><span>This study aim to isolat</span><span lang="IN">e</span><span> and characterize Pb resistant of heterotrophic bacteria in Cilalay Lake, </span><span lang="IN">West Java, </span><span>Indonesia. The water samples were collected </span><span lang="IN">along</span><span> three points around Cilalay Lake. </span><span lang="IN">Water physical and chemical </span><span>determination was performed using the Water Quality Checker</span><span lang="IN">. </span><span>The bacterial isolates were screened on T</span><span lang="IN">r</span><span>ipton</span><span lang="IN">e</span><span> Glucose Yeast (TGY) agar plates. </span><span lang="IN">Afterwards s</span><span>elected isolates were grown on Nutrient Agar media 50% </span><span lang="IN">with </span><span>supplemented Pb 100 ppm by the standard disk. Population of resistant bacteria was counted. The result from metal resistant bacteria indicated that all isolates w</span><span lang="IN">ere</span><span> resistant. The most abundant type of resistant </span><span lang="IN">bacteria </span><span>to lead was Gram negative more than Gram positive. Identified have metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water</span>

scholarly journals Microbiological Screening and Antimicrobial Sensitivity Profiling of Wound Infections in a Tertiary Care Hospital of Bangladesh

2020 ◽  
pp. 101-106
Keyword(s):  
Wound Infection ◽  
Primary Care Physicians ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Treatment Modalities ◽  
Care Hospital ◽  
Bacterial Isolates ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Sensitivity

Wound infection is a major problem in hospitals in developing countries. Wound infection causes morbidity and prolonged hospital stay thus this prospective study was conducted for a period of seven months (January 2019 to July 2019). A total of 217 specimens (wound swabs and pus exudates) from wound infected patients in a Tertiary Care Hospital in Bangladesh. A retrospective study of the microbiological evaluation was done by cultural growth as well as Gram staining and biochemical examination to identify the bacterial isolates. Finally, the antimicrobial vulnerability testing was performed by Kirby-Bauer disc diffusion conventional method. A total of 295 samples were tested. Out of which 217 (73.5%) were found culture positive. E. coli was the most predominant gram-negative isolates whereas Staphylococcus aureus and Coagulase-negative Staphylococcus were the most commonly isolated gram-positive organisms. Antimicrobial sensitivity profile of bacterial isolates revealed imipenem, meropenem, amikacin, and nitrofurantoin to be the most effective antimicrobials against gram-negative isolates, whereas imipenem, meropenem, amikacin, nitrofurantoin, amoxiclav, and gentamicin were the most effective drugs against gram-positive isolates. The result of this examination contributes to the identification of basic causative microbes involved in wound infection and findings of antibiotic susceptibility patterns can be helpful for primary care physicians to optimize the treatment modalities, articulate policies for empiric antimicrobial therapy, and to minimize the rate of infection among wound infected patients.

scholarly journals Antimicrobial Resistance Patterns of Bacterial Isolates from Blood Culture among HIV/AIDS Patients at Felege Hiwot Referral Hospital, Northwest Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Mohabaw Jemal ◽  
Teshiwal Deress ◽  
Teshome Belachew ◽  
Yesuf Adem
Keyword(s):  
Antimicrobial Resistance ◽  
Blood Culture ◽  
Agar Plate ◽  
Blood Cultures ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Aids Patients ◽  
Gram Positive ◽  
Gram Negative ◽  
Hiv Aids

Background. The emergence and spread of antimicrobial resistance in bacteria is recognized as a global public health problem. Bloodstream infection with antimicrobial-resistant bacteria in HIV/AIDS patients makes the problem more challenging. So, regular and periodic diagnosis and use of the appropriate antimicrobial susceptibility pattern determination is the only option for decreasing the prevalence and development of drug-resistant bacteria. Methods. An institution-based cross-sectional study was conducted among 384 HIV/AIDS patients. Sociodemographic data of patients were recorded using structured questionnaires. Blood cultures were collected with BACTEC aerobic blood culture bottles. A pair of samples was collected from each patient aseptically and incubated at 37°. If samples are positive for bacterial agents, they were subcultured to solid media such as blood agar plate, chocolate agar plate, and MacConkey agar plates. Identification was performed using colony characteristics and standard biochemical techniques. The antimicrobial susceptibility test was determined by the Kirby–Bauer disc diffusion method. Data entry and analysis were performed while using SPSS version 20. Descriptive statistics were performed to calculate frequencies. Results. Altogether, 384 patients were included, and 123 blood cultures were positive, so that the yield was thus 32%. About 46 (37.4%) of Gram-negative and 77 (62.6%) of Gram-positive bacterial species were identified. Among Gram-negative bacterial isolates, K. pneumoniae was the leading pathogen, 19 (41.3%), whereas S. aureus, 38 (49.4%), was predominant among Gram-positive isolates. In his study, the majority of Gram-positive isolates showed high level of resistance to penicillin, 72 (95.5%), tetracycline, 55 (71.4%), and cotrimoxazole, 45 (58.4%). About 28 (73.6%) of S. aureus isolates were also methicillin-resistant. Gram-negative bacterial isolates also showed a high resistance to ampicillin (91.3%), tetracycline (91.3%), and gentamicin (47.8%). Overall, about 78% of multidrug resistance was observed. Conclusion. Several pathogens were resistant to greater than five antimicrobial agents, so that proper management of patients with bacteremia is needed, and a careful selection of effective antibiotics should be practiced.

scholarly journals Isolation and quantification of indicator and pathogenic microorganisms along with their drug resistance traits from bottled and jar water samples within Dhaka city, Bangladesh

2020 ◽  
Vol 9 (1) ◽  
pp. 12-14
Author(s):  
Md Aftab Uddin ◽  
Mst Aysha Siddiqua ◽  
Mst Sadia Ahmed
Keyword(s):  
Water Samples ◽  
Bacterial Count ◽  
Fecal Coliforms ◽  
Pathogenic Microorganisms ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Potential Threat ◽  
Antibiotic Susceptibility Pattern ◽  
Shigella Spp

Commercial drinking water may serve as potential threat to public health if these items are contaminated with a number of pathogenic microorganisms due to faulty manufacturing process. Present study attempted to isolate and quantify the microorganisms from various jar and bottle water samples collected from various areas of Dhaka city. Antibiotic susceptibility pattern of suspected bacterial isolates were also determined in this study. Out of the eighteen samples studied, ten were jar water samples and eight were bottled water samples. The range of total viable bacterial count (TVBC) in these samples ranged from 102 to 105 cfu/ml. Specific pathogens such as, Salmonella spp., Shigella spp., Vibrio spp. and fecal coliforms could not be found in these samples. However coliforms could be detected in 10 samples. The antibiogram study showed that all Escherichia coli and Klebsiella spp. isolates found from these samples were sensitive against gentamicin (10 μg) and azithromycin (30 μg). Variable antibiotic resistance among these bacterial isolates was detected against cefotaxime (30 μg), streptomycin (10 μg) and erythromycin (15 μg). Stamford Journal of Microbiology, Vol.9(1) 2019: 12-14

scholarly journals Bakteri Aerob Penyebab Infeksi Nosokomial di Ruang Bedah RSU Abepura, Kota Jayapura, Papua

2018 ◽  
Vol 4 (2) ◽  
pp. 63-68
Author(s):  
Daniel Lantang ◽  
Desi Paiman
Keyword(s):  
Listeria Monocytogenes ◽  
Nosocomial Infection ◽  
Brain Heart Infusion ◽  
Aerobic Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Agar Media ◽  
Identification Of Bacteria

The research on identification of aerobic bacteria causing nosocomial infection in the surgery room of General Hospital (RSU) Abepura, Jayapura had been done from August–December 2008. Samples were taken from room air (30 minutes) using blood agar media and from certain tools at some points using brain heart infusion media (BHI). The colony appearance was observed after 24 hours of incubation period of bacterial growth. Further analysis to identify the aerobic bacteria causing nosocomial infections was done by several tests in Jayapura Health Laboratory. The result showed that there were 15 aerobic bacteria consisted of: the coccid gram–positive bacteria: Staphylococus epidermidis, S. aureus, S. saprophyticus, Streptcoccus sp; the rod gram-positive bacteria: Listeria monocytogenes, Diptheroid sp, Lactobacillus sp and gram-negative bacteria: Providence rettgeri, Pseudomonas puttrefaciens, Klebsiela ozaena, P. malthophyla, Morganela morganii, Serattia sp, K. oxytoca, and K. pneumonia. Key words: Aerobic bacteria, identification of bacteria, nosocomial infection, surgical room, Jayapura.

scholarly journals Detection of microbial proliferation & assessment of antibacterial activity of spices collected from various locations of Dhaka city, Bangladesh

2017 ◽  
Vol 7 (1) ◽  
pp. 10-13
Author(s):  
Md Aftab Uddin ◽  
Khondakar Wahid Hasan ◽  
Jubaida Binta Jamal
Keyword(s):  
Antibacterial Activity ◽  
Zingiber Officinale ◽  
Bacterial Count ◽  
Syzygium Aromaticum ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Two Samples ◽  
Microbial Proliferation ◽  
Staphylococcus Spp

The current study was aimed to detect the presence of contaminating microorganisms in some commonly available spices collected from different areas of Dhaka city, Bangladesh and also to assess their antibacterial activity. Out of eight samples under this study, total viable bacterial count (TVBC) was observed within the range of 106 to 107cfu/g. Among the specific bacterial isolates Staphylococcus spp., Pseudomonas spp. and Escherichia coli were found in almost all the samples within the margin of 102 to 106 cfu/g. Alarmingly Salmonella spp. and Vibrio spp. were presumptively identified in one and two samples respectively. Besides every sample showed the presence of fungus with load up to 103 cfu/g. Antibacterial activity of these samples was tested against seven laboratory isolates. Syzygium aromaticum showed highest activity against as many as five bacterial isolates while Cinnamomum verum was active against four isolates. On the other contrary, both Zingiber officinale and Laurus nobilis demonstrated their antibacterial activity only against Staphylococcus spp. and Klebsiella spp. respectively. Stamford Journal of Microbiology, Vol.7(1) 2017: 10-13

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