scholarly journals Critical Quality Control Issues of Antibiotic Susceptibility Tests for Developing Countries

2021 ◽  
pp. 17-21
Author(s):  
Ayodele Oluwaseun Ajayi ◽  
Oluwabukola Atinuke Popoola
Keyword(s):  
Developing Countries ◽  
Antibiotic Susceptibility ◽  
Bacterial Infections ◽  
Antibiotic Resistant ◽  
Treatment Regimens ◽  
Treatment And Prevention ◽  
Antibiotic Susceptibility Test ◽  
Susceptibility Tests ◽  
And Control ◽  
Quality Assurance And Control

Antibiotic therapy has been the mainstay of treatment of bacterial infections and the initial successes of the earlier generations of antibiotics for treatment and prevention of bacterial infections spurred further efforts to discover newer antibiotics. Antibiotic Susceptibility Test (AST) is a standardized procedure usually carried out on cultivable bacterial pathogens to determine their sensitivity to specific antibiotics, and to guide physicians on the best antibiotic treatment regimens for specific infections. The quality assurance and control for antibiotic susceptibility tests should be prioritized in deliberate efforts to reduce the burden of antibiotic resistant bacterial infections in developing countries.

scholarly journals All-electrical monitoring of bacterial antibiotic susceptibility in a microfluidic device

2020 ◽  
Vol 117 (20) ◽  
pp. 10639-10644 ◽  
Author(s):  
Yichao Yang ◽  
Kalpana Gupta ◽  
Kamil L. Ekinci
Keyword(s):  
Microfluidic Device ◽  
Electrical Resistance ◽  
Antibiotic Susceptibility ◽  
Bacterial Infections ◽  
Morphological Changes ◽  
Resistant Bacteria ◽  
Microfluidic Channels ◽  
Cross Sectional ◽  
Antibiotic Susceptibility Test ◽  
Susceptibility Tests

The lack of rapid antibiotic susceptibility tests adversely affects the treatment of bacterial infections and contributes to increased prevalence of multidrug-resistant bacteria. Here, we describe an all-electrical approach that allows for ultrasensitive measurement of growth signals from only tens of bacteria in a microfluidic device. Our device is essentially a set of microfluidic channels, each with a nanoconstriction at one end and cross-sectional dimensions close to that of a single bacterium. Flowing a liquid bacteria sample (e.g., urine) through the microchannels rapidly traps the bacteria in the device, allowing for subsequent incubation in drugs. We measure the electrical resistance of the microchannels, which increases (or decreases) in proportion to the number of bacteria in the microchannels. The method and device allow for rapid antibiotic susceptibility tests in about 2 h. Further, the short-time fluctuations in the electrical resistance during an antibiotic susceptibility test are correlated with the morphological changes of bacteria caused by the antibiotic. In contrast to other electrical approaches, the underlying geometric blockage effect provides a robust and sensitive signal, which is straightforward to interpret without electrical models. The approach also obviates the need for a high-resolution microscope and other complex equipment, making it potentially usable in resource-limited settings.

Antibiotic susceptibility pattern against pathogenic bacteria causing Dental Caries

2011 ◽  
Vol 1 (1) ◽  
pp. 31-35
Author(s):  
Deepak Dwivedi ◽  
Tejram Kushwah ◽  
Mukesh Kushwah ◽  
Vinod Singh
Keyword(s):  
Dental Caries ◽  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Resistant Bacteria ◽  
Susceptibility Pattern ◽  
Antibiotic Resistant ◽  
Antibiotic Susceptibility Pattern ◽  
Penicillin V ◽  
Antibiotics Susceptibility ◽  
Susceptibility Tests

Antibiotics to treat dental caries infection are routinely prescribed which led to the increased resistance against bacteria. The purpose of this investigation was to perform antibiotic susceptibility tests on a panel of pathogenic bacteria isolated from dental caries infection. Bacteria were isolated from caries site of patients and identified at the species level. Each of 150 species of bacteria was tested for antibiotics susceptibility to a five antibiotics using Etest. The antibiotics used were Amoxicillin, Cloxocillin, Erythromycin, Tetracycline and Penicillin‐V. The obtained resistance percentage for each antibiotic were Penicillin V: 72/150 (48%), Tetracycline: 99/150 (66%), Amoxicillin: 135/150 (90%), Cloxocillin: 117/150 (78%), and Erythromycin: 90/150 (60%) (Table 1). In case of combinatorial antibiotic exposure, the resistance percentage of Penicillin V/Amoxicillin and Amoxicillin/ Erythromycin was 39/150 (26%), and 45/150 (30%) respectively. The study has well demonstrated the clinical picture of antibiotic resistance and susceptibility pattern of bacteria causing dental caries. The obtained comprehensive data will allow investigating the spatial distribution of pathogenic, antibiotic resistant bacteria among dental caries patients which further may help into development of novel diagnostic and treatment approaches for the same.

scholarly journals Microbial Isolates from Vegetable Foreign Bodies Inhaled by Dogs

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Sara Flisi ◽  
Manuel Dall’Aglio ◽  
Costanza Spadini ◽  
Clotilde Silvia Cabassi ◽  
Fausto Quintavalla
Keyword(s):  
Respiratory Tract ◽  
Antibiotic Susceptibility ◽  
Resistant Bacteria ◽  
Upper Respiratory Tract ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Bacteria And Fungi ◽  
Susceptibility Tests ◽  
The Right

Grass-seed inhalation is a common problem in canine patients, in particular during summer months, migrating in upper and lower respiratory tract. Grass awns can harbor bacteria and fungi, causing grass seeds foreign body-related disease (GSFBD). Aim of this study was to investigate the aerobic microbial flora isolated from grass awns extracted from 41 dogs with GSFBD and the antibiotic susceptibility of the isolated bacterial strains. Fifty-four grass awns were localized with diagnostic imaging tests and removed by endoscopy from respiratory tract. The most frequent localizations were in the left nostril and the right hemithorax. Only one grass awn was extracted from each patient except in 7 that had more than one. Bacteriological and mycological cultures, strains identification, and antibiotic susceptibility tests were performed. One or more bacterial strains were isolated from all grass awns. Fungal strains were isolated only in 4 cases. Staphylococcussp. was the most frequent isolate in the upper respiratory tract (36.8%), whileE. coli(24.4%) was the most frequent isolate in the lower tract. Fluoroquinolones and Doxycycline were the most effective antibiotics, while resistance was observed against Gentamicin (>93%), Cefapirin, and Clindamycin (>80%). These data are relevant in relation to the use of these antibiotics in both animals and humans, for the risk of transmission of antibiotic resistant bacteria or resistance genes.

scholarly journals Antibiotic susceptibility and pathogenicity of Aeromonas hydrophila isolated from red hybrid tilapia (Oreochromis niloticus×Oreochromis mossambicus) in Malaysia

2020 ◽  
Vol 13 (10) ◽  
pp. 2166-2171 ◽  
Author(s):  
Nurfarahin Ahmad Pauzi ◽  
Nurliyana Mohamad ◽  
Mohamad Azzam-Sayuti ◽  
Ina Salwany Md. Yasin ◽  
Mohd Zamri Saad ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Bacterial Infections ◽  
Infected Fish ◽  
The Body ◽  
Limited Information ◽  
Tubular Necrosis ◽  
Wide Range ◽  
Mar Index ◽  
Susceptibility Tests

Background and Aim: Aeromonas hydrophila is a major cause of bacterial infections affecting a wide range of warm water fishes worldwide. In Malaysia, A. hydrophila isolations from diseased fishes were previously reported; however, with limited information. The present study investigates the antibiotic susceptibility and pathogenicity of A. hydrophila isolated from farmed red hybrid tilapia (Oreochromis spp.) in Malaysia. Materials and Methods: A. hydrophila was biochemically identified and subjected to antibiotic susceptibility tests. The isolate was then intraperitoneally injected into red hybrid tilapia, and the mortality, clinicopathological changes, and LD50 were determined up to 240 h post-infection (hpi). Results: The isolate demonstrated multiple antibiotic resistances (MAR) toward amikacin, ampicillin, cefotaxime, amoxicillin, trimethoprim-sulfamethoxazole, erythromycin, and streptomycin, with a MAR index of 0.5. The experimental infection of A. hydrophila at 105 CFU/mL in the red hybrid tilapia resulted in 100% mortality at 240 hpi. The LD50 was determined at 1.1×104 CFU/mL. Infected fish demonstrated occasional erratic swimming patterns, localized hemorrhages and depigmentation on the body and operculum areas, fin erosion, enlargement of the gall bladder, and hemorrhage in internal organs. Microscopic observation of infected fish revealed brain congestion, tubular necrosis, and glomerular shrinkage in the kidneys, necrosis of hepatocytes, and congestion of blood vessels in the liver. Conclusion: The high virulence of A. hydrophila to the red hybrid tilapia emphasizes the importance of active, on-going monitoring of its prevalence in Malaysian tilapia farming.

scholarly journals Beating the Bio-Terror Threat with Rapid Antimicrobial Susceptibility Testing

2021 ◽  
Vol 9 (7) ◽  
pp. 1535
Author(s):  
Shahar Rotem ◽  
Ida Steinberger-Levy ◽  
Ofir Israeli ◽  
Eran Zahavy ◽  
Ronit Aloni-Grinstein
Keyword(s):  
Antibiotic Susceptibility ◽  
Environmental Samples ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Development Stage ◽  
Post Exposure Prophylaxis ◽  
Bacterial Agent ◽  
Susceptibility Tests ◽  
Exposure Prophylaxis ◽  
And Control

A bioterror event using an infectious bacterium may lead to catastrophic outcomes involving morbidity and mortality as well as social and psychological stress. Moreover, a bioterror event using an antibiotic resistance engineered bacterial agent may raise additional concerns. Thus, preparedness is essential to preclude and control the dissemination of the bacterial agent as well as to appropriately and promptly treat potentially exposed individuals or patients. Rates of morbidity, death, and social anxiety can be drastically reduced if the rapid delivery of antimicrobial agents for post-exposure prophylaxis and treatment is initiated as soon as possible. Availability of rapid antibiotic susceptibility tests that may provide key recommendations to targeted antibiotic treatment is mandatory, yet, such tests are only at the development stage. In this review, we describe the recently published rapid antibiotic susceptibility tests implemented on bioterror bacterial agents and discuss their assimilation in clinical and environmental samples.

Nanomedicine and Its Applications in Combating Resistance Relating to Opportunistic Pathogens With Special Reference to Tuberculosis

2021 ◽  
pp. 87-113
Author(s):  
Priya Shrivastava ◽  
Laxmikant Gautam ◽  
Anamika Jain ◽  
Nikhar Vishwakarma ◽  
Sonal Vyas ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Special Reference ◽  
Bacterial Infections ◽  
Term Therapy ◽  
Intracellular Pathogens ◽  
Antibiotic Resistant ◽  
Potential Applications ◽  
Long Term Therapy ◽  
And Control

The worldwide misuse of antimicrobials and subsequent rise of the multi-drug resistant (MDR) opportunistic intracellular pathogens have led to a paradigm shift in the established perspective of antimicrobials and bacterial-human relations. Antibiotic resistant strains of mycobacterium tuberculosis have threatened the progress in management and control of worldwide epidemic of tuberculosis. Mycobacterium tuberculosis intrinsically resists many antimicrobials, limiting the number of compounds available for treatment. Typically, conventional treatments for these diseases consist of long-term therapy with a combination of bioactive(s) that can cause side effects and contribute to low patient compliance. The escalation of antimicrobial resistance poses compelling demand for new ways of treating bacterial infections. The main objective of this chapter is to review the potential applications of nanotechnology-based drug delivery systems in combating drug resistance associated with opportunistic intracellular pathogens with special reference to tuberculosis.

scholarly journals Occurrence of Antibiotic Resistant Bacteria in Environmental Wastes

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 151-157
Author(s):  
R.C. Poudel ◽  
D.R. Joshi ◽  
N.R. Dhakal ◽  
A.B. Karki
Keyword(s):  
Staphylococcus Aureus ◽  
Sewage Sludge ◽  
Antibiotic Susceptibility ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Antibiotic Susceptibility Pattern ◽  
Antibiotic Susceptibility Test ◽  
Enterococcus Spp

Microbial resistance to antibiotics has been emerging in environmental isolates. This study was carried out from October 2008 to January 2009 to describe the antibiotic susceptibility pattern of the bacteria isolated from sewage sludge, biowaste and bioslurry samples. A total of 49 identified isolates were taken for antibiotic susceptibility test. Amikacin and Gentamicin were the effective antibiotics for the Gram negative bacteria, comparatively Escherichia coli was the most sensitive. Similarly, all isolates of Staphylococcus aureus and Enterococcus spp. were sensitive to Tetracycline and Erythromycin respectively and none of these isolates were resistant to Vancomycin. Out of 13 S. aureus, five (38.5%) were Methicillin resistant Staphylococcus aureus (MRSA). The multidrug resistant (MDR) isolates accounted for 46.9%, the highest percentage of MDR isolates was seen in Pseudomonas aeruginosa (9/12, 75.0%). The proper management of multidrug resistant bacteria present in waste is suggestive for environmental and public health.Key words: Multidrug resistant bacteria, Bio-waste, Sewage sludge, BioslurryDOI: 10.3126/on.v7i1.2563Our Nature (2009) 7:151-157 

scholarly journals Isolation, Identification, and Antibiotic Sensitivity Pattern of Salmonella spp from Locally Isolated Egg Samples

2019 ◽  
pp. 1-11 ◽  
Keyword(s):  
Antibiotic Susceptibility ◽  
Public Health Problem ◽  
Resistance Pattern ◽  
Human Consumption ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
Poultry Eggs ◽  
Sensitivity Pattern ◽  
Antibiotic Susceptibility Test

Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered as major sources for these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg sample collected from different retailer markets and farm of Savar area, Bangladesh. Antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from eggs yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found to Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from egg surface were found more antibiotic resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.

Microbial spectrum and antibiotic susceptibility of uropathogens causing Urinary tract infection in pregnant women

2011 ◽  
Vol 1 (2) ◽  
pp. 77-80
Author(s):  
Pallavi Jayavanth ◽  
Junainah Abd Hamid ◽  
Mathan Mohan ◽  
Mohamed Rafi ◽  
Rajasegar Anamalley ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Antibiotic Susceptibility ◽  
Antibiotic Resistant ◽  
Isolation And Characterization ◽  
Antimicrobial Surveillance ◽  
Antibiotic Susceptibility Test ◽  
Urine Specimens

Urinary tract infection (UTI) is an intermittent cause of sickness in the pregnant women. A study was conducted to identify the microbial gamut and antibiotic vulnerability patterns of bacteria isolated from pregnant women with suspected urinary tract infection. In total, 75 urine specimens were screened for bacteriuria over a period of four months. Aseptically collected urine specimens were subjected to standard isolation and characterization procedures. Antibiotic susceptibility test was performed according to CLSI (2007). Five types of bacteria were isolated namely Escherichia coli 32(55%), Staphylococcus aureus 12 (21%), Enterococcus Sp 7 (12%), Klebseilla pneumoniae 4 (7%) and Pseudomonas aeruginosa 3 (5%). All bacterial isolates exhibited efficacy to all the antibiotics namely fosfomycin (50mg), ciprofloxacin (10mg), trimethoprim & sulfamethoxazole (25 mg), ampicillin (10 mg), nitrofurantoin (300 mg) and cefazolin (30mg) except amoxycillin. Ampicillin and ciprofloxacin exhibited illustrious efficacy at low concentration. Regular antimicrobial surveillance and reinforcement of appropriate antibiotic regimen in pregnant women with UTI will restrain the re‐emergence of antibiotic resistant uropathogens.

scholarly journals A Phage Therapy Guide for Clinicians and Basic Scientists: Background and Highlighting Applications for Developing Countries

2021 ◽  
Vol 11 ◽  
Author(s):  
Ali Khalid ◽  
Ruby C. Y. Lin ◽  
Jonathan R. Iredell
Keyword(s):  
Developing Countries ◽  
Low Income ◽  
Bacterial Infections ◽  
Disease Burden ◽  
Phage Therapy ◽  
Critical Level ◽  
Low Income Countries ◽  
Global Health Research ◽  
Antibiotic Resistant ◽  
Feasible Option

Approximately 10% of global health research is devoted to 90% of global disease burden (the so-called “10/90 Gap”) and it often neglects those diseases most prevalent in low-income countries. Antibiotic resistant bacterial infections are known to impact on healthcare, food security, and socio-economic fabric in the developing countries. With a global antibiotic resistance crisis currently reaching a critical level, the unmet needs in the developing countries are even more striking. The failure of traditional antimicrobials has led to renewed interest in century-old bacteriophage (phage) therapy in response to the urgent need to develop alternative therapies to treat infections. Phage therapy may have particular value in developing countries where relevant phages can be sourced and processed locally and efficiently, breaking specifically the economic barrier of access to expensive medicine. Hence this makes phage therapy an attractive and feasible option. In this review, we draw our respective clinical experience as well as phage therapy research and clinical trial, and discuss the ways in which phage therapy might reduce the burden of some of the most important bacterial infections in developing countries.

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