scholarly journals Isolation of Diarrhea Causing Organisms (Salmonella and Shigella) from Selected Seafood

Author(s):  
Owhorchukwu Amadi- Wali ◽  
Chinyere Amadi- Wali ◽  
Allwell Sunny Njigwum

Aims: The study investigated the presence of entities causing diarrhea (Shigella & Salmonella) from some selected seafood. Study Design:  The study adopted a completely randomized experimental Design. Place and Duration of Study: Department of Medical Microbiology, Rivers State University Teaching Hospital (RSUTH), between January 2020 and February 2020. Methodology: Simple random technique was employed to collect sufficient quantities of five different fresh raw seafoods (shrimp, periwinkle, crab, sardine fish and mudskipper) across fish harbors and fish markets (Nembe waterside, Abonema Wharf and 1 Fish Market) in Port Harcourt, and were evaluated for bacteriological quality. Sample collection was a cross-sectional type. The isolation and identification of isolates were done according to standard bacteriological analytical methods. The study employed Frequency counts, percentages and one- way ANOVA statistics, and the analysis was done using SPSS version 23. Although, one-way ANOVA statistics was used to test the hypothesis of the study at 0.05 level of significance, while Tukey’s test was used for ranking means. Results: The finding showed that 53 percent of the isolates (i.e. 8 out of 15 isolates) were characterized as Salmonella and Shigella. Also, the result shows that all the seafood evaluated contain unacceptable levels of Salmonella and Shigella contamination, which ranged from 1.79 x107 CFU/g to 2.96 x107CFU/g. The level of contamination found in the selected seafood is shown in descending order from the highest to the lowest: Sardine> Periwinkle> Shrimps> Mudskipper> Crab. More so, result from the hypothesis showed that there was a significant mean difference in the Salmonella and Shigella count amongst selected seafood (P < .001). Conclusion: The results of this study constitute an indicator of fecal contamination in selected seafood from fish markets in Port Harcourt. Amongst others, it was recommended that Government should enforce laws discouraging the dumping of untreated waste into water bodies.

2021 ◽  
Vol 10 (1) ◽  
pp. 218-223
Author(s):  
Kalio DGB ◽  
Eli S ◽  
Okagua KE ◽  
Allagoa DO

Background: Post-operative anaemia is often a reflection of pre-operative pre-operative work-up and pre-operative anaemia. In addition. Post-operative anaemia is also determined by co-morbidities of patients prior to surgery. The prevalence of post-operative anaemia varies based on surgical specialties and the experience of the surgeon; prevalence rates as high as 85% have been recorded in orthopaedic surgeries. Aim: To determine the prevalence of post-operative anaemic in surgical patients at the Rivers State University Teaching Hospital (RSUTH). Method: This was a six months cross sectional study of the post-operative anaemia of patients who had operation at the Surgery and Obstetrics/Gyaecology departments of The Rivers State University Teaching Hospital. The permission for the study was granted by the head of department of obstetrics and gynaecology in conjunction with the head of .the hospital management. The yard stick for anaemia was packed cell volume less than 33% in line with the World Health Organization (WHO). A structured proforma was used to obtain information from patient’s case notes and analyzed using SPSS version 25. Result: Three hundred and eigthy subjects were recruited for the study. Males subjects were 150 (39.5%) while females were 230 (60.5%) respectively. The mean age was 31 years. One hundred and ninety nine (52.4%) were obstetrics and gynaecological surgeries while 181 (47.6 %) were non-gynaecological surgeries. The commonest indication for surgery was caesarean section representing 130 (34.2%) of the subjects. Two hundred and sixty six of the subjects (70%) had PCV less than 33%. One hundred and fifty two (40%) women had PCV less than 33% while 114 (30%) of the men had PCV less than 33%. Conclusion: The study revealed that prevalence of post-operative anaemia amongst surgical patients at RSUTH was 70 %. The post-operative anaemia amongst women was worrisome. The need to optimize patients prior to surgery cannot be over-emphasized to prevent morbidities and mortalities post-operative.


Author(s):  
Eze Chinwe Nwadiuto ◽  
Ihua Nnenna ◽  
Ijewere Blessing Ofureb

In the abattoir, large amount of water is being used in processing operations; and this produces huge amount of wastewater which empty into the surrounding water bodies. The cross-sectional study followed the conventional microbiological techniques of macroscopy, microscopy, isolation, and identification to assess the bacteriological and parasitological examination of water sources in selected abattoirs in Port Harcourt Metropolis, Rivers State, Nigeria. The result of microbial population showed Total Coliform ranging from 5 x 101cfu/ml – 2.80 x 102cfu/ml and Faecal Coliform bacteria of 2.80 x 101 cfu/ml -1.85 x 102 cfu/ml. Total Heterotrophic bacteria ranged from 7.8 x101cfu/ml to 4.2 x102cfu/ml. The mean microbial counts; 4.86 x101 cfu/ml Fecal Coliform, 9.65 x 101cfu/ml Total Coliform and 1.82 x 102cfu/ml Heterotrophic Count. The isolates identified were Klebsiella spp, Shigella spp., Salmonella typhymurium, E. coli, Proteus spp., Salmonella paratyphi, Vibrio cholera. Nkpor village abattoir recorded highest occurrence of bacteria isolate with a total of 50(22.03%) followed by Iwofe 48(21.15%). Water sample from ponds had a higher parasitic contamination while samples from the borehole were free from parasitic infestation. Water samples for parasitological assessment were filtered and examined for parasitic organism using standard methods. Four different parasites were identified in the study, they include Ascaris lumbricoides (32.5%), Entamoeba histolytica (22.5%), Giardia lamblia (17.5%) and the least Taenia spp (7.5%). Nkpor-village abattoir had the highest level of contamination with (30.0%) parasite occurrence, followed by Mgboshimini abattoir (15.0%). The use of unsafe water for meat processing by butchers, portends a serious public health risk to consumers who purchase the meat, from these abattoirs. Therefore, government should endeavour to make borehole water available to all the abattoirs in Port Harcourt.


Author(s):  
T. Neebee–Sordum ◽  
D. G. Tamuno-emine ◽  
D. Onwuli

Aim: The aim of this study was to assess thyroid hormone levels in among diabetics in Port- Harcourt  of Rivers State in Nigeria Study Design: This study is a cross-sectional study. Place and Duration of Study: This study was conducted at Chemical Pathology Department, Rivers State University Teaching Hospital, Port Harcourt, Nigeria, between December, 2019 and February, 2020. Methodology: A total of 224 subjects were recruited for the study, diabetic parents (132 subjects), non-diabetic parents (112 subjects. Five (5) ml of blood sample was collected and used to analyse for serum thyroxine (T4), tri-iodothyronine (T­­3) and thyroid stimulating hormone (TSH) using Enzyme-linked immunoasorbent assay. Data were analyzed statistically with SPSS version 22.0 and values considered significant at p< 0.05. Results: The mean ± S.D of serum TSH were 1.28± 0.6u/ml (control) and 1.84±1.05 u/ml (subject), (p=0.023). T4 value were 2.49 ± 0.86u/ml (control) (p=0.024) and 3.90 ±1,62u/ml (subjects) and T3 values were 1.89±0.41u/ml (p=0.0001). Conclusion: The evaluation of thyroid hormones (TSH, T4 and T3) among diabetes with the age ranges of population studied shows that there was altered thyroid hormone in diabetes mellitus individuals.


Author(s):  
Felix Chikaike Clement Wekere ◽  
Rose Sitonma Iwo-Amah ◽  
Joseph Ngozi Kwosah ◽  
Adetomi Bademosi ◽  
Simeon Chijioke Amadi

Background: Caesarean section (CS) is a major obstetric surgery done for pregnant women and is lifesaving.  Aim: The study was aimed at reviewing CS at the Rivers State University Teaching Hospital (RSUTH), to determine the prevalence, trend and indications, for improved management outcomes. Methods: In this cross-sectional study, we reviewed five thousand, five hundred and ninety-eight (5598) cases of caesarean sections (3699 emergency, 1899 planned) managed at the RSUTH from 1st January, 2015 to 31st December, 2019.  Data collected were analysed using IBM, Statistical Product and Service Solutions (SPSS), version 25.0 Armonk, New York. Results: There were 13,516 deliveries and 5,598 cases of CS over the review period, giving the prevalence of CS as 41.4% or 414 per 1000 deliveries.  Emergency and planned CS cases accounted for 66.1% and 33.9% respectively. An increasing trend of CS was observed over the review period. The mean age ± SD of the participants was 32.30±1.04 years (95% CI:30.26,34.34). Modal age group was 35-39 years, accounting for 33.2% followed by those aged 30-34 years (26.2%). Majority of the patients were multipara [3396 (60.7%)], married [4890 (87.4%)], Christians [5540 (99%)] and had tertiary level education [2800 (50%)]. The commonest indication for CS in RSUTH was previous caesarean section [1925(34%)], followed by cephalopelvic disproportion [757(13.4%)], foetal distress [418(7.4%)], preeclampsia [390(6.9%)] and multiple gestation [252 (4.5%)]. Conclusion: The rate of caesarean section is high in RSUTH with an increasing trend.  Although CS is lifesaving, efforts should be made to reduce the rate to the level recommended by WHO, especially in Low-middle-income countries (LMICs), where there is high aversion to CS.


2021 ◽  
Vol 42 (2) ◽  
pp. 403-410
Author(s):  
M.A. Erasmus ◽  
G.N. Wokem

A cross-sectional investigation of hepatitis-B status among attendees of Modern Primary Health Centre, Eneka and Rivers State University Teaching Hospital, Port Harcourt was evaluated. Seven hundred (700) subjects of different ages of both sexes were included in the study after ethical approval was obtained from the Rivers State Ministry of Health, Port Harcourt. Consent forms were issued to get subjects’ consent before questionnaire administration to obtain their demographic data. The uninfected subjects were used as control. About 4mls of blood was taken from each subject by vein-puncture; 2mls each was dispensed into EDTA and plain bottles for analysis. The samples were used to assay for hepatitis-B surface antigen (HBsAg), hepatitis–B virus (HBV) markers (HBsAb, HBeAg, HBeAb, HBcAb) using standard techniques. The overall prevalence of HBV was 5.1% in Port Harcourt. The males had higher prevalence of 7.9% HBV than the females 3.4% HBV, although there was no statistically significant difference (P ≥0.05). The HBV was highest among subjects of age group 24-29 years (8.29%) and 30-35 years (9.2%) accordingly. The HBV markers results show that while HBsAg occurred among all the subjects that were positive for HBV, HBeAb was completely absent.


Author(s):  
Peter A. Awoyesuku ◽  
Dagogo A. Mac Pepple

Background: The high maternal and perinatal mortality rates in Nigeria continue to be issues of concern. That antenatal care improves both perinatal and maternal outcomes is now well established. The study seeks to identify the predictors of poor maternal and perinatal outcome among singleton maternal delivery cases referred to Rivers State University Teaching Hospital (RSUTH).Methods: A longitudinal study was employed; the patients referred to RSUTH were followed up to ascertain maternal and neonatal outcomes. A sample size of 460 was used. Selection of cases was done by systematic sampling. Data obtained in the study were demographic, obstetric and perinatal findings. Maternal and perinatal outcome were dichotomized into poor and good outcomes. Bivariate and multivariate analyses were performed using SPSS version 20.0.Results: A total of 460 cases were recruited. The mean age±SD was 28.7±4.6 years. There was poor maternal outcome in 65 (14.1%) and poor perinatal outcome in 291 (63.3%). There was one maternal death (rate 0.2%), perinatal mortality rate was 26.5%, low birth-weight rate was 6.3% and asphyxia rate was 23.3%. There is an association of multiparity and unbooked status with poor maternal outcome, while poor perinatal outcome was influenced by unbooked status.Conclusions: Perinatal and maternal mortality were high amongst all referral cases. There is a positive correlation of multiparity with poor maternal outcome and between unbooked status and an increased risk of both maternal and perinatal adverse outcomes.


2021 ◽  
Vol 11 (3) ◽  
pp. 115-119
Author(s):  
Eli S ◽  
Aaron FE ◽  
Okagua KE ◽  
Omodu OJ ◽  
Iyama AC

Background: With the high prevalence of HIV in Sub-Saharan Africa, screening for this viral infection is a routine in many health institutions prior to surgery, the world has recorded success story in the research of HIV especially with the advent of Highly Active Anti-Retroviral Therapy (HAART) this disease burden has been reduced globally. The prevalence of HI in Nigeria is 1.4%. Aim: To determine the prevalence of HIV in surgical patients at the Rivers State University Teaching Hospital (RSUTH), Port Harcourt, Rivers State, Nigeria. Method: This was a cross sectional study of ANC attendees at the RSUTH, from January 1st to June 31st, 2019. They were surgical patients who were systematically selected, information was obtained from patients case notes with a structured proforma. The information obtained was coded and analyzed using SPSS version 25. Result: A total of 370 patients were attended to pre-operatively. There were 146 (39.5%) males and 224 (60.5%) females. The mean age was 31 years. One hundred and ninety four (52.4%) were obstetrics and gynaecological surgeries while 176 (47.6 %) were non-gynaecological surgeries. The commonest indication for surgery was caesarean representing 126 (34.1%) of the subjects.The prevalence of HIV in surgical patients was 8 (2.1%). Three (1.6%) of the obstetrics and gynaecological patients had HIV compared to 1(0.5%) non-obstetrics and gynaecological patient. Conclusion: The study revealed that prevalence of HIV in surgical patients at the RSUTH was high (2.1%). HIV infection was thrice as common in obstetrics and gynaecological patients compared with other surgical disciplines. There is need to step up surveillance amongst ANC attendees who are reflection of the populace in this region.


Author(s):  
Mandu A. March ◽  
Hannah A. Vaikosen ◽  
Enye A. Akporoghene

The study investigated information needs and seeking behaviour of nurses at Rivers State University Teaching Hospital (RSUTH), Port Harcourt. It adopted a descriptive research survey. The population of the study was 352 nurses at RSUTH from which a sample 100 was drawn. Self-structured questionnaire was used. The questionnaire included a four point likert scale (4-1). Out of 100 copies of the questionnaire administered, 94 were found valid for analysis. Data was analysed using simple percentages and mean score. The findings of the study showed that nurses needed information on drugs therapy, diagnostic, patients’ management and personal development. The factors that motivated their information seeking include working environment, patients’ care, professionalism and personal development. To satisfy their curiosity, they consulted different sources of information including colleagues, internet and textbook. It also showed that nurses had a negative perception about library and information services in meeting their information needs. Inadequate information resources in the library, inadequate information retrieval skills and closed access were some of the challenges they faced when seeking for their information needs. However, they suggested training of nurses and provision of standard library for the nurses. It was recommended among other things that the library should be well equipped to meet the information needs of the nurses also the nurses should acquire information retrieval skills to enable them access information resources on the internet and databases. Keywords:  Information needs, Information seeking behaviour, Nurses, Rivers, Health information


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