scholarly journals Antioxidant Capacity and Total Phenolic and Flavonoid Contents of Methanolic Extracts of Urtica dioica L. by Different Extraction Techniques

2020 ◽  
pp. 207-214
Author(s):  
Sabina Begić ◽  
Emir Horozić ◽  
Hurija Alibašić ◽  
Edita Bjelić ◽  
Salih Seferović ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Soxhlet Extraction ◽  
Biologically Active ◽  
Total Phenolic ◽  
Bioactive Components ◽  
Extraction Techniques ◽  
Active Components ◽  
Methanolic Extracts ◽  
High Antioxidant Activity

In this study, the efficacy of different extraction techniques (maceration, ultrasound-assisted and Soxhlet extraction) on the content of biologically active components in extracts from fresh and dried nettle leaves, and their antioxidant activity were analyzed. Methanol was used as the solvent. Total phenolic content and antioxidant capacity were determined by Folin-Ciocalteu, DPPH and FRAP methods, respectively. High content of total phenolic compounds and high antioxidant activity were recorded in extracts of dried nettle. Extracts obtained from fresh nettle samples showed significantly lower content of analyzed bioactive components and lower antioxidant activity. In the case of all extracts, Soxhlet extraction proved to be the most efficient, and maceration the least efficient extraction technique for isolation of bioactive components from nettle leaves.

scholarly journals Anti-urease, total phenolic content and antioxidant activities of some Inula l. (Asteraceae) taxa in Turkey

Genetika ◽  
2020 ◽  
Vol 52 (3) ◽  
pp. 825-834
Author(s):  
Emre Sevindik ◽  
Sinem Aydin ◽  
Mehmet Paksoy ◽  
Bahar Sokmen
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Soxhlet Extraction ◽  
Total Phenolic ◽  
Food Industries ◽  
Total Antioxidant ◽  
Ethanol Extracts

The genus Inula belongs to family Asteraceae and it is medicinally important because it possesses cytotoxic, anticancer, hepatoprotective, antibacterial and anti-inflammatory properties. The aim of this research was to explore antioxidant and anti-urease activities of fifteen Inula taxa in Turkey. Ethanol extracts of Inula taxa were obtained by Soxhlet extraction. Antioxidant activity was determined through total antioxidant activity, total phenolic content and copper reducing antioxidant capacity (CUPRAC) analyses. The highest total phenolic content and total antioxidant capacity were found in I. orientalis (158.54?0.002 ?g GAE/mL) and I. macrocephala (189.16?0.002 ?g AAE/mL). CUPRAC activity increases with the increasing concentration of the extracts and the standard. Moreover, all the tested plants exhibited better anti-urease activity than standard thiourea except for I. anatolica and I. thapsoides subsp. thapsoides. Results obtained from this study demonstrate that Inula species might be utilized as a probable natural source in the pharmaceutical and food industries but further and more specific studies are needed to determine active antioxidative and anti-urease compounds in the extracts.

scholarly journals EVALUATION OF TOTAL POLYPHENOLS AND ANTIOXIDANT CAPACITY IN MUSHROOM EXTRACTS PLEUROTUS OSTREATUS AND LENTINULA EDODES

2020 ◽  
pp. 96-99
Author(s):  
IVAN MARCELO GARCIA ◽  
ARTURO ROJAS S. ◽  
DANILO MONTERO ◽  
MARCO ARELLANO
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Pleurotus Ostreatus ◽  
Lentinula Edodes ◽  
Ethanolic Extract ◽  
Total Polyphenols ◽  
Methanolic Extracts ◽  
High Antioxidant Activity ◽  
Mushroom Extracts

Objective: This research was aimed at assessing the concentration of total polyphenols in ethanolic and methanolic extracts of Pleurotus ostreatus and Lentinula edodes mushrooms and antioxidant activity. Methods: Polyphenols were determined by the Folin Ciocalteu method, using lyophilized mushroom samples for the preparation of extracts and antioxidant activity by the TBARS method. Results: Extracts prepared from mushrooms showed appreciable values ​​of polyphenols, and for the ethanolic extract of Pleurotus ostreatus and Lentinula edodes values ​​of 102.78 and 81.83 mg of gallic acid/100 g of the sample respectively, comparable to those obtained in some fruits For methanolic extracts, values ​​of 100.45 and 78.92 mg of gallic acid/100 g of sample were obtained. Polyphenol concentration values ​​for the Pleurotus were higher in the two types of extracts and lower for the Lentinula edodes. Conclusion: When evaluating the antioxidant activity, high antioxidant activity was found for the two types of mushroom, Pleurotus ostreatus and Lentinula edodes, presenting peroxidase inhibition values ​​of 88.04 and 89.49% respectively.

scholarly journals Total polyphenol content and antioxidant capacity of allium plants and their correlation with antibacterial and antifungal activity

2021 ◽  
pp. 85-97
Author(s):  
S. S. Satorov ◽  
F. D. Mirzoeva
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Biologically Active ◽  
Diffusion Method ◽  
Active Components ◽  
Total Polyphenols ◽  
Disk Diffusion Method ◽  
Ethanol Extracts ◽  
Antibacterial And Antifungal

Aim. To determine the total polyphenols and antioxidant capacity and their correlation with antimicrobial and fungicidal properties in some species of the genus Allium growing in Tajikistan.Materials and methods. The objects of the study were 15 kinds of the Amaryllidaceae family. Antibacterial onions were studied by the disk diffusion method against 4 types of microorganisms (test strains) and 1 type of yeast fungus. In the presence of total polyphenols, the content in μg of Gallic acid (GA) per ml of plant extract was measured. To determine the antioxidant activity, a solution of ABTS K2S2O8 was used. Trolox was used as a standard.Results. The maximum content of total polyphenols is characterized by ethanol extracts of A. Carolinianum, A. altaicum, A. elatum, A. oschaninii, A. ramosum, A. hymenorhisum. At the same time, their lowest amount was found in the bulbs of A. longicuspis. A. shoenoprasum, which is statistically significantly lower than in all the above-listed species of this plant group. The extracts from the stems of A. carolinianum, A. elatum, and A. pamiricum had the highest antioxidant effect. There is a direct weak correlation between biologically active components with antimicrobial and fungicidal activity in A. oshaninii and A. suworowii, depending on the altitude gradient.Conclusions. The results allow suggesting that the above-ground and underground parts of some wild-growing endemic species of the genus Allium have different degrees of antioxidant activity due to total polyphenols. The content of total polyphenols and the antioxidant capacity of extracts obtained from various plants in different in large ranges. In some cases, there are direct correlations between the content of polyphenols, antioxidant properties, antimicrobial and fungal properties.

scholarly journals PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF THE CROATIAN RED CLOVER GERMPLASM COLLECTION

Poljoprivreda ◽  
2020 ◽  
Vol 26 (2) ◽  
pp. 3-10
Author(s):  
Daniela Horvat ◽  
◽  
Marijana Tucak ◽  
Marija Viljevac Vuletić ◽  
Tihomir Čupić ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Germplasm Collection ◽  
Red Clover ◽  
Biologically Active ◽  
Total Phenolic ◽  
Active Components ◽  
Leaf Extracts ◽  
Breeding Populations ◽  
Full Flowering

The leaf samples of two varieties, twenty breeding populations, and seven local populations of the Croatian red clover were collected in the full flowering stage, with an aim to evaluate their phenolic content and antioxidant activity by using the spectrophotometric methods. A significant variation among the varieties/populations in the content of total phenolics and flavonoids was determined. The results demonstrated that the red clover is a rich source of phenolics and flavonoids, ranging from 38.67 to 59.96 mg GAE/g of DM and 21.19 to 51.48 mg CE/g of DM, respectively. The high levels in both phenolics and flavonoids were found in breeding populations/variety CD-8, Rc-11/7, Rc-11/8, Rc-11/15 and OS Osiris. The leaf extracts manifested a strong antioxidant activity towards diphenyl-1-picrylhydrazyl (DPPH), with a variation from 31.50 to 63.14%. A significant correlation was found between the antioxidant activity of the extracts and their total phenolic and flavonoid content (r = 0.737 and 0.839, respectively). Considering the obtained results, the crude extracts of red clover manifested a significant antioxidant potential and can be used as a natural source of biologically active components in the human and animal nutrition.

scholarly journals Value-Added Pastry Cream Enriched with Microencapsulated Bioactive Compounds from Eggplant (Solanum melongena L.) Peel

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 351
Author(s):  
Georgiana Horincar ◽  
Elena Enachi ◽  
Vasilica Barbu ◽  
Doina Georgeta Andronoiu ◽  
Gabriela Râpeanu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Solanum Melongena ◽  
Value Added ◽  
Biologically Active ◽  
Total Phenolic ◽  
Overall Acceptability ◽  
A Value ◽  
High Antioxidant Activity ◽  
Weak Gel

In this study, antioxidant-rich eggplant peel extract was used to obtain a value-added pastry cream. In order to reduce the susceptibility to degradation, microencapsulation of the biologically active compounds from the eggplant peel was first performed. The microencapsulated bioactive compounds powder (MBC) obtained through freeze-drying retained about 94.31% of the anthocyanins present in the extract, was rich in phenolic compounds, and displayed a high antioxidant activity. The purple colored powder was added to the pastry cream in different concentrations (5% and 10%), allowing significant increase of total phenolic content and antioxidant activity, which were rather stable over 72 h of storage under refrigeration conditions. Sensory evaluation indicated that addition of MBC resulted in improved color and overall acceptability of the pastry cream formulation. All pastry cream samples exhibited rheological behavior specific to the weak gel-like structures, with increasing values of storage modulus with MBC addition. The instrumental texture analysis showed that MBC addition to the pastry cream slightly decreased the firmness and improved the chewiness of the samples.

scholarly journals Variation in Phenolic Compounds Content and Antioxidant Activity of Different Plant Organs from Rumex crispus L. and Rumex obtusifolius L. at Different Growth Stages

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 237 ◽  
Author(s):  
Pavel Feduraev ◽  
Galina Chupakhina ◽  
Pavel Maslennikov ◽  
Natalia Tacenko ◽  
Liubov Skrypnik
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Reproductive Organs ◽  
Biologically Active ◽  
Total Phenolic ◽  
Growth Stages ◽  
Active Components ◽  
Rumex Crispus ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

The study investigated the accumulation of phenolic compounds and the antioxidant activity of extracts of various parts of R. crispus and R. obtusifolius, collected at the flowering stage and the fruiting stage. Half of the collected plants were divided into root, stem, leaves, and reproductive organs (inflorescence). The other half was used to study the vertical distribution of biologically active components and antioxidants throughout the plant. The samples were analyzed for total catechins content, total proanthocyanidins content, total phenolic content, and total antioxidant activity (1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’azinobis(3)ethylbenzthiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays). All analyses were performed in four replicates. In general, a similar trend was observed in the distribution of phenolic compounds in the studied species. The maximum content of these secondary metabolites was noted in the reproductive organs, both in the flowering and fruiting period. Stems were characterized by a minimum content of the studied classes of substances. The antioxidant activity of the sorrels studied parts can be arranged in the following order: the generative part (flowers, seeds) > leaves > root > stem (for flowering and fruiting stages). It was found that parts of the root closer to the stem differed in higher activity.

scholarly journals Some important physicochemical and bioactive characteristics of the main apricot cultivars from Turkey

2020 ◽  
Vol 44 (6) ◽  
pp. 651-661
Author(s):  
Neva KARATAŞ ◽  
Memnune ŞENGÜL
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Titratable Acidity ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Fruit Peel ◽  
Positive Effects ◽  
Trolox Equivalent ◽  
High Antioxidant Activity ◽  
Β Carotene

Turkey is one of the world’s leading producers in both in fresh and dried apricots, and Malatya Province is the capital for apricots. In the present study, the fruit of 13 apricot cultivars (Adilcevaz, Alkaya, Aprikoz, Çataloğlu, Hacıhaliloğlu, Hasanbey, İsmailağa, Kabaaşı, Mahmuduneriği, Soğancı, Şam, Şekerpare, and Tokaloğlu-Erzincan), grown together at the Apricot Research Institute in Malatya Province, were harvested and evaluated for some important physicochemical and bioactive characteristics. The results showed statistically significant differences in most of the physicochemical and bioactive characteristics. Fresh apricot fruit peel color values, L,a, and b, were determined as between 48.66 and 64.70, 8.12and 22.82, and 16.50and 38.67, respectively. The fruit, the dry matter (DM), pH, titratable acidity, reducing sugar, sucrose, total sugar, total phenolic content, lycopene, β-carotene, vitamin A, vitamin E and vitamin C contents were between 13.05% and 23.12%, 3.68 and 5.04, 0.22% and 1.40%, 2.02 and 5.40 g/100 g, 1.83 and 3.97 g/100g, 4.96 and 8.04 g/100 g, 24.60 and 50.69 mg GAE/mg fresh weight, 3.84 and 17.89 mg/100g, 19.59 and 40.53 mg/100g, 0.13 and 0.67 μg/g, 15.67 and 22.12 μg/g, and 1.41 and 8.16 μg/g, respectively. Antioxidant activity was determined using 3 different methods, β-carotene bleaching, trolox equivalent antioxidant capacity (TEAC), and 2,2-Diphenyl-1-picrylhydrazyl hydrate (DPPH). The antioxidant capacity varied according to the methods used. The results showed that apricot fruit had high antioxidant activity and varied according to methods used, and was rich in carotenoids and phenolic substances, which have positive effects on human health and can be consumed as a functional food.

EXTRACTION OPTIMIZATION AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS FROM AVOCADO PEEL

2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Quadratic Model ◽  
Total Phenolic ◽  
Total Antioxidant

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel

scholarly journals Optimization of Natural Antioxidants Extraction from Pineapple Peel and Their Stabilization by Spray Drying

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1255
Author(s):  
Sofia C. Lourenço ◽  
Débora A. Campos ◽  
Ricardo Gómez-García ◽  
Manuela Pintado ◽  
M. Conceição Oliveira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Spray Drying ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Food Products ◽  
Total Phenolic ◽  
Dry Extract ◽  
Pineapple Peel ◽  
Dpph Method

Pineapple peel still contains an important amount of phenolic compounds and vitamins with valuable antioxidant activity. In this way, the aim of this study was the recovery of the bioactive compounds from pineapple peel using environmentally friendly and low-cost techniques, envisaging their application in food products. From the solid-liquid extraction conditions tested, the one delivering an extract with higher total phenolic content and antioxidant capacity was a single extraction step with a solvent-pineapple peel ratio of 1:1 (w/w) for 25 min at ambient temperature, using ethanol-water (80–20%) as a solvent. The resulting extract revealed a total phenolic content value of 11.10 ± 0.01 mg gallic acid equivalent (GAE)/g dry extract, antioxidant activity of 91.79 ± 1.98 µmol Trolox/g dry extract by the DPPH method, and 174.50 ± 9.98 µmol Trolox/g dry extract by the FRAP method. The antioxidant rich extract was subjected to stabilization by the spray drying process at 150 °C of inlet air temperature using maltodextrin (5% w/w) as an encapsulating agent. The results showed that the antioxidant capacity of the encapsulated compounds was maintained after encapsulation. The loaded microparticles obtained, which consist of a bioactive powder, present a great potential to be incorporated in food products or to produce bioactive packaging systems.

scholarly journals Antifungal and Antioxidant Potential of Methanolic Extracts from Acorus calamus L., Chlorella vulgaris Beijerinck, Lemna minuta Kunth and Scenedesmus dimorphus (Turpin) Kützing

2021 ◽  
Vol 11 (11) ◽  
pp. 4745
Author(s):  
Toncho Dinev ◽  
Milena Tzanova ◽  
Katya Velichkova ◽  
Diyana Dermendzhieva ◽  
Georgi Beev
Keyword(s):  
Antioxidant Activity ◽  
Chlorella Vulgaris ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Acorus Calamus ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Methanolic Extracts ◽  
Scenedesmus Dimorphus

Plant extracts are an important alternative to antibiotics, which are ever more restricted because of their developing microbial resistance and some adverse effects that have been observed following frequent application. The aim of the present study was to determine the antifungal and antioxidant activity of the methanolic extracts of Acorus calamus, Chlorella vulgaris, Lemna minuta and Scenedesmus dimorphus. The antifungal activity of the extracts against strains of Aspergillus flavus, Aspergillus parasiticus, Aspergillus ochraceus, Aspergillus niger, Aspergillus carbonarius, Fusarium graminearum, Fusarium oxysporum, Penicillium chrysogenum and Alternaria alternata was evaluated via the agar well diffusion method. The antioxidant activity of the extracts was measured through the determination of three parameters—total phenolic content, total flavonoid content and radical scavenging potential (determined through UV/Vis analysis). A. calamus extracts had the highest antimicrobial activity against eight fungal strains, followed by the C. vulgaris, L. minuta and S. dimorphus extracts, which were inhibitory against two to three strains. Among the extracts from the species studied, the extract from S. dimorphus showed the highest antioxidant potential, as determined via the DPPH (1,1’-diphenyl-2-picrylhydrazil-radical) method. This correlated to its high total phenolic and flavonoid content. From A. calamus and L. minuta, methanolic extracts were obtained that exhibited similar values of the aforementioned parameters, followed by C. vulgaris extracts, which showed the lowest antioxidant activity. Based on the Pearson correlation coefficients, the impacts of the total phenolic content and the total flavonoid content on radical scavenging capacity are similar, and flavonoids were a significant part of the total phenolic compounds extracted from the plant materials studied.

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