Composition of Fatty Acids and Antioxidant Activity of Pomegranate Seed Oil CV. 'Molar'

2019 ◽  
pp. 1-9
Author(s):  
Ágda Malany Forte De Oliveira ◽  
Railene Hérica Carlos Rocha Araújo ◽  
Kalinny de Araújo Alves ◽  
Elny Alves Onias ◽  
Roberlúcia Araújo Candeia ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Fruit Development ◽  
Seed Oil ◽  
Biologically Active ◽  
Punicic Acid ◽  
Omega 3 ◽  
Pomegranate Seed Oil ◽  
Randomized Design ◽  
Pomegranate Seed

Aims: Pomegranate has been used since ancient times as a universal therapeutic agent due to the presence of biologically active ingredients in different parts of the plant. Pomegranate seed oil is considered a nutraceutical because of its rich composition. Therefore, this work aimed to study the main changes in the composition of fatty acids and antioxidant activity of pomegranate seed oil (cv. Molar) in different stages of fruit development. Study design: Completely randomized design. The treatments were the ages (60, 70, 80, 90 and 100 days), counted from the beginning of the anthesis. For each harvest a random sampling of five fruits was used for each repetition, and four replications per stage of fruit development were performed totaling 20 fruits per treatment. Place and Duration of Study: The research was carried out in partnership with the farm Águas de Tamanduá, located in Várzeas de Sousa, PB, (longitude 38°13'41" and latitude 06°45'33"). Methodology: The characterization of the phenological phases of pomegranate (Molar cv.) development was carried out at the beginning of the orchard. Vigorous and healthy adult plants were selected. Hermaphrodite flowers were marked, evenly distributed in the area, with colored tape resistant to high temperature, sunshine, winds and rains. The marking of the flowers occurred in the early hours of the morning, and at the time of the marking, thinning of flowers was carried out on branches that had two or more flowers at the apex, leaving only a single flower on the branch. Seed oil was extracted from a sample of 20 fruits at different stages of development: 60, 70, 80, 90 and, 100 days counted from the start of the anthesis. Results: The general composition of the oil of pomegranate seeds cv. Molar, regardless of the stage of fruit development, takes the order of PUFA> SFA> MUFA, with a higher content of polyunsaturated fatty acids (omega 3 and 6), and after saturated and monounsaturated, and low concentrations of total Trans Isomers. Conclusion: The best periods for the consumption of pomegranate seed oil are between 80 and 90 days due to the higher amount of unsaturated acids and punicic acid, and lower concentrations of palmitic acid, as well as a higher concentration of phenolic compounds. The method of DPPH, with methanol extractor identifies the antioxidant activity of pomegranate seed oil, however not efficiently.

scholarly journals Fatty Acids Composition of Red and Purple Pomegranate (Punica granatum L) Seed Oil

2010 ◽  
Vol 1 (2) ◽  
pp. 74 ◽  
Author(s):  
Hartati Soetjipto ◽  
Murda Pradipta ◽  
KH Timotius
Keyword(s):  
Fatty Acids ◽  
Neutral Lipid ◽  
Acid Content ◽  
Seed Oil ◽  
Lipid Fraction ◽  
Punica Granatum ◽  
Punicic Acid ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed ◽  
Punica Granatum L

The aim of this investigation was to determine the content and composition of fatty acid in seed oil of red and purple pomegranate (Punica granatum L). The extraction process was performed by Soxhlet extractor with petroleum ether as solvent. The separation and identification of pomegranate seed oil was done by using GCMS. The total oil content of red and purple  pomegranate  were 128 g/kg d.w  and 103 g/kg d.w respectively. Both showed the same major fatty acids  as palmitic, stearic, oleic, linoleic and  punisic acid. Oleic acid (19-21%) and linoleic acid (20-21%) were found as the most  dominant fatty acids in red pomegranate, whereas purple pomegranate seed oil was dominated by oleic acid (41-43%) and punicic acid  (0-25%). Neutral lipid  fraction of  red and purple pomegranate seed oils was more dominant than glycolipid and phospholipid. Neutral  lipid fraction  of red and purple pomegranate seed oil were  89 % and 91% respectively.  Glycolipid fraction  of red and purple pomegranate seed oil were 8 % and 5 %, whereas phosholipid fraction of red and purple pomegranate seed oil were 3 % and 4 %. The punicic acid content of  total lipid of  purple pomegranate seed oil (PPSO) (0-25%) was higher than red pomegranate (RPSO) (9-16%). On the contrary neutral lipid of red pomegranate showed higher punicic acid content (54-75%) than the purple pomegranate (14-55%). Glycolipid of red pomegranate contained  punicic acid  (0-42%). The punicic acid content  of the phospholipid fraction of  red pomegranate was higher (0-22 %) than the one of purple pomegranate (0-2%).Key words : fatty acid, pomegranate, Punica granatum, punicic acid, seed oil

Evaluation of green extraction processes, lipid composition and antioxidant activity of pomegranate seed oil

2021 ◽  
Author(s):  
E. Rojo-Gutiérrez ◽  
O. Carrasco-Molinar ◽  
J. M. Tirado-Gallegos ◽  
A. Levario-Gómez ◽  
M. L. Chávez-González ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Lipid Composition ◽  
Seed Oil ◽  
Green Extraction ◽  
Pomegranate Seed Oil ◽  
Extraction Processes ◽  
Pomegranate Seed

Pomegranate seed oil: Effect on 3-nitropropionic acid-induced neurotoxicity in PC12 cells and elucidation of unsaturated fatty acids composition

2016 ◽  
Vol 20 (1) ◽  
pp. 40-48 ◽  
Author(s):  
Bushra N. Al-Sabahi ◽  
Majekodunmi O. Fatope ◽  
Musthafa Mohamed Essa ◽  
Selvaraju Subash ◽  
Saleh N. Al-Busafi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Pc12 Cells ◽  
Seed Oil ◽  
Unsaturated Fatty Acids ◽  
Fatty Acids Composition ◽  
Pomegranate Seed Oil ◽  
Nitropropionic Acid ◽  
Pomegranate Seed ◽  
3 Nitropropionic Acid

scholarly journals Oil content, lipid profiling and oxidative stability of “Sefri” Moroccan pomegranate (Punica granatum L.) seed oil

OCL ◽  
2021 ◽  
Vol 28 ◽  
pp. 5
Author(s):  
Ahmed Hajib ◽  
Issmail Nounah ◽  
Hicham Harhar ◽  
Said Gharby ◽  
Badreddine Kartah ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Oxidative Stability ◽  
Oil Content ◽  
Seed Oil ◽  
Saturated Fatty Acids ◽  
Punica Granatum ◽  
Major Constituent ◽  
Punicic Acid ◽  
Pomegranate Seed

The aim of this study was to determine the chemical composition (fatty acids, tocopherols, and sterols) and evaluate the oxidative stability of Moroccan pomegranate (Punica granatum L.) seed oil. The oil content of pomegranate seed was 22.63 g/100g of dry weight. The fatty acid composition showed a dominance of conjugated linolenic acids (CLnAs) (86.96 g/100g). The most dominant fatty acid was punicic acid (75.1 g/100g), followed by catalpic acid (6.7 g/100g) and linoleic acid with amounts of 4.11 g/100g. The seed oil only contained a low level of saturated fatty acids with palmitic (2.64 g/100g) and stearic acids (1.73 g/100g) as main saturated fatty acids. The sterol marker, β-sitosterol, accounted for 404.59 mg/100g of the total sterol content in the seed oil. Total tocopherol content in seed oil was 332.44 mg/100g. γ-tocopherol (190.47 mg/100g oil) is the major constituent, followed by α-tocopherol (74.62 mg/100g oil) and δ-tocopherol (53.3 mg/100g oil). The induction time calculated by the Rancimat accelerated method was found to be of 3.6 h at 120 °C. In terms of oil, pomegranate seed oil may be considered as a valuable source for new multipurpose products with industrial, cosmetic and pharmaceutical uses.

scholarly journals Effect of pomegranate seed oil on fatty acids composition of Oreochromis niloticus trough supplemented diet

2019 ◽  
Vol 41 (1) ◽  
pp. 37995 ◽  
Author(s):  
Vanessa Vivian de Almeida Schneider ◽  
Maria Eugênia Petenuci ◽  
Ana Paula Lopes ◽  
Vanessa Jorge Santos ◽  
Jesuí Vergílio Visentainer
Keyword(s):  
Fatty Acids ◽  
Oreochromis Niloticus ◽  
Seed Oil ◽  
Fatty Acids Composition ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed

Pomegranate seed oil, a rich source of punicic acid, prevents diet-induced obesity and insulin resistance in mice

2011 ◽  
Vol 49 (6) ◽  
pp. 1426-1430 ◽  
Author(s):  
Irene O.C.M. Vroegrijk ◽  
Janna A. van Diepen ◽  
Sjoerd van den Berg ◽  
Irene Westbroek ◽  
Hiskias Keizer ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Seed Oil ◽  
Rich Source ◽  
Punicic Acid ◽  
Pomegranate Seed Oil ◽  
Diet Induced Obesity ◽  
Pomegranate Seed

scholarly journals Effect of the Biodegradable Coatings the Base on Microalgae and Oil of the Seed of the Pomegranate in the Conservation Powder-Crop of the Papaya ‘Golden’

2018 ◽  
Vol 10 (10) ◽  
pp. 367 ◽  
Author(s):  
Albert E. M. de M. Teodosio ◽  
Railene H. C. R. Araujo ◽  
José F. de Lima ◽  
Elny A. Onias ◽  
Ana P. N. Ferreira ◽  
...  
Keyword(s):  
Seed Oil ◽  
Promising Alternative ◽  
Pomegranate Seed Oil ◽  
Chlorella Sp ◽  
Randomized Design ◽  
Pomegranate Seed ◽  
Completely Randomized Design ◽  
Biodegradable Coatings ◽  
Crop Conservation

It is very challenging to the quality of the papaya culture’s fruits (Carica papaya L.) after the crop, especially due to their significance to the international market, that is, their elevated exportation demands. The purpose of this work was the application of biodegradable coatings composed of Scenedesmus sp. and Chlorella sp. associated or not with pomegranate seed oil in ‘Golden’ papaya and to evaluate their effect in the conservation powder-crop. The installation of the experiment was carried out in a completely randomized design, with a 6 x 6 factorial outline, that is, six concentrations (C: control; SO: 0.5% of Scenedesmus sp. + 0.3% of pomegranate seed oil; S: 0.5% of Scenedesmus sp.; CO: 0.5% of Chlorella sp. + 0.3% of pomegranate seed oil; CH: 0.5% of Chlorella sp.; O: 0.3% of pomegranate seed oil) and six evaluation periods (0, 3, 6, 9, 12, and 15 days), stored at a temperature of 18±2 °C with 60±5% RH with three repetitions of two fruits per portion. The use of coverings composed of Scenedesmus sp. and Chlorella sp. in association or not with pomegranate seed oil was proven efficient in the reduction of the breathing tax of ‘Golden’ papaya, delaying the ripening process, and therefore representing a promising alternative for these fruits’ powder-crop conservation. The coating composed of 0.5% of Chlorella sp. + 0.3% of pomegranate seed oil (CO) provided a better powder-crop conservation of ‘Golden’ papaya during 15 days of storage.

Pomegranate Seed Oil Modulates Functions and Survival of BV-2 Microglial Cells in vitro

2014 ◽  
Vol 84 (5-6) ◽  
pp. 295-309 ◽  
Author(s):  
Lucia Račková ◽  
Volkan Ergin ◽  
Elif Burcu Bali ◽  
Marcela Kuniaková ◽  
Çimen Karasu
Keyword(s):  
Seed Oil ◽  
Therapeutic Potential ◽  
Fold Increase ◽  
Punica Granatum ◽  
Current Evidence ◽  
No Production ◽  
Omega 3 ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed

Abstract. Current evidence has demonstrated the immunomodulatory efficacy of omega-3 polyunsaturated fatty acids (PUFAs) in glial cells, suggesting their therapeutic potential for diseases in the central nervous system (CNS). However, conjugated omega-5 PUFAs have also attracted considerable attention because of their suggested anti-inflammatory effects. In the present study, the effect of pomegranate (Punica granatum L.) seed oil (PSEO) (a rich source of omega-5 PUFAs) on the activation of cultured BV-2 microglia was investigated within a 24-hour incubation period. PSEO (25 μg/ml) showed only a slightly smaller inhibitory effect on LPS-stimulated NO production (243 ± 12.5 % of control, p<0.001 vs. 437 ± 9.2 % in stimulated cells) and TNF-α release (87.1 ± 5.62 pg/ml vs. 229 ± 24.4 pg/ml in stimulated cells), as well as iNOS expression (7.36-fold of control, p < 0.01, vs. 17.5-fold increase in stimulated cells) compared to a standardized omega-3 PUFAs mixture (25 μg/ml) and the flavonoid quercetin (25 μmol/l). Unlike quercetin and stobadine, only the PUFA preparations effectively prevented apoptosis of microglia (as confirmed by the suppression of caspase 3 activation) exposed to the toxic concentration of LPS. The PUFA preparations did not provide a notable suppression of the intracellular oxidant generation and did not influence the intracellular distribution of cholesterol (as confirmed by filipin staining). However, they appeared to affect the morphology of activated cells. In conclusion, our data point to the first evidence of immunomodulation and cytoprotection of BV-2 microglia by the pomegranate seed oil, indicating that it may be (comparably to omega-3 PUFAs) efficient against microglia-mediated neuroinflammation while preventing the premature depletion of these immune effector cells in the brain.

scholarly journals Effect of pomegranate seed oil on hyperlipidaemic subjects: a double-blind placebo-controlled clinical trial

2010 ◽  
Vol 104 (3) ◽  
pp. 402-406 ◽  
Author(s):  
Parvin Mirmiran ◽  
Mohammad Reza Fazeli ◽  
Golaleh Asghari ◽  
Abbas Shafiee ◽  
Fereidoun Azizi
Keyword(s):  
Clinical Trial ◽  
Seed Oil ◽  
Lipid Profiles ◽  
Controlled Clinical Trial ◽  
Punicic Acid ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Pomegranate Seed Oil ◽  
Baseline Values ◽  
Pomegranate Seed

In vitroandin vivostudies have shown that punicic acid, a type of conjugated fatty acid and the main constituent of pomegranate seed oil (PSO), has anti-atherogenic effects. The present study aimed at determining the effect of PSO treatment on serum lipid profiles. This double-blind placebo-controlled randomised clinical trial included fifty-one hyperlipidaemic subjects, diagnosed according to National Cholesterol Education Program definition, and randomly assigned to the PSO and the control groups. The PSO and placebo groups received 400 mg PSO and placebo twice daily, respectively and were followed up for 4 weeks. Serum concentrations of lipids and lipoproteins were measured before and 4 weeks after intervention. Mean concentration of TAG and the TAG:HDL cholesterol (HDL-C) ratio were significantly decreased after 4 weeks in the PSO group as compared with baseline values (2·75 (sd1·40)v. 3·45 (sd1·56) mmol/l,P = 0·009 and 5·7 (sd4·6)v. 7·5 (sd5·0),P = 0·031, respectively). The treatment effect was statistically significant in the PSO group as compared with controls in diminution of cholesterol:HDL-C ratio (5·4 (sd1·5)v. 5·9 (sd1·4),P < 0·05) adjusted for baseline values. We found a mean difference for PSOv.placebo in HDL-C concentration (0·13 v. − 0·02 mmol/l) and cholesterol:HDL-C ratio ( − 0·42 v. 0·01,P < 0·05). Serum cholesterol, LDL cholesterol and glucose concentrations and body composition variables remained unchanged. It is concluded that administration of PSO for 4 weeks in hyperlipidaemic subjects had favourable effects on lipid profiles including TAG and TAG:HDL-C ratio.

scholarly journals Pomegranate Seed Oil and Bitter Melon Extract Affect Fatty Acids Composition and Metabolism in Hepatic Tissue in Rats

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5232
Author(s):  
Agnieszka Stawarska ◽  
Tomasz Lepionka ◽  
Agnieszka Białek ◽  
Martyna Gawryjołek ◽  
Barbara Bobrowska-Korczak
Keyword(s):  
Fatty Acids ◽  
Dietary Supplements ◽  
Seed Oil ◽  
Dietary Factors ◽  
Female Rats ◽  
Hepatic Tissue ◽  
Bitter Melon ◽  
Fatty Acids Composition ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed

Pomegranate seed oil (PSO) and bitter melon dried fruits (BME) are used as natural remedies in folk medicine and as dietary supplements. However, the exact mechanism of their beneficial action is not known. The aim of study was to assess how the diet supplementation with PSO and/or with an aqueous solution of Momordica charantia affects the metabolism of fatty acids, fatty acids composition and the level of prostaglandin E2 (PGE2) in rat liver. Animals (Sprague-Dawley female rats, n = 48) were divide into four equinumerous groups and fed as a control diet or experimental diets supplemented with PSO, BME or both PSO and BME for 21 weeks. Fatty acids were determined using gas chromatography with flame ionization detection. PSO added to the diet increased the rumenic acid content (p < 0.0001) and increased accumulation of n-6 fatty acids (p = 0.0001) in hepatic tissue. Enrichment of the diet either with PSO or with BME reduced the activity of Δ6-desaturase (D6D) (p = 0.0019), whereas the combination of those dietary factors only slightly increased the effect. Applied dietary supplements significantly reduced the PGE2 level (p = 0.0021). No significant intensification of the influence on the investigated parameters resulted from combined application of PSO and BME. PSO and BME have potential health-promoting properties because they influence fatty acids composition and exhibit an inhibiting effect on the activity of desaturases and thus they contribute to the reduction in the metabolites of arachidonic acid (especially PGE2).

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