scholarly journals Distribution of Aromatic Volatiles in Young, Recently Matured, Matured and Old Leaves of Ginger (Zingiber officinales) by Electronic Nose

Author(s):  
Ramasamy Ravi ◽  
. Sunil ◽  
Dharma Pitchay

Aims: Ginger aromatic volatiles generally extracted and quantified by various laboratory techniques analysis and instruments. Possibility of few highly volatiles loss coupled with the formation of different compounds during various extraction process due to changes in the natural chemical profiles of the ginger tissue samples. Thus, a study was carried out to explore and quantify the distribution of various natural volatiles present in young, recently matured, matured and old leaves of ginger. Study Design: Ginger seedlings and plants were grown in 100% biochar substrate and fertigated with 50 mg.L-1 of 20% N:4.3% P:16.6% K water soluble fertilizer. Five months old ginger plant leaves were harvested and washed with distilled water and separated by various physiological leaf stages. Place and Duration of Study: Tennessee State University, Nashville, USA. Methodology: Aromatic volatiles were analyzed and quantified by using Alpha Soft V14 electronic nose. Volatiles with more than 1% concentration by fresh mass were 20, 21, 20, and 29 in young, recently matured, matured and old leaves respectively. Results: Young, recently matured, matured and old leaves of ginger showed major volatiles  like benzaldehyde, 1, 8- cineole, myrcene, butane-2, 3-dione, 1 S-α- pinene, Z-3-Hexen-1-ol acetate, butanol and 1-Propanal, 2-methyl- volatiles were identified. A significant variation in the composition, quantity and distribution of volatiles was found across the various leaf tissue samples of ginger. Conclusion: The distribution of aromatic volatiles in this study would provide better insight and guidance to the consumers for maximum utilization ginger plant tissues beside rhizome; as a source of aromatic volatiles. Utilization of other plant tissues especially could be considered as an important byproduct will definitely contribute to the sustainability of ginger production and marketing in the near future.

1992 ◽  
Vol 10 (1) ◽  
pp. 52-54
Author(s):  
Judith R. Purman ◽  
Francis R. Gouin

Abstract Rooted cuttings of poinsettia (Euphorbia pulcherrima Willd. ex Clotzsch ‘Amy’) and seedlings of bibb lettuce (Lactusa sativa L.), baldhead cabbage (Brassica oleracea L. var. Capitata), sweet william (Dianthus barbatus L.) and pansy (Viola & Wittrockiana Gams.) were grown in Sunshine Mix and 7 experimental media containing 30-day-old (“New”) or 90-day-old (“Aged”) cocompost (polymer dewatered sewage sludge composted with processed garbage), perlite or vermiculite, and peatmoss. Plants were fertilized biweekly with water soluble fertilizer of 25N-2.2P-16.6K (25-5-20) at 250 ppm of N applied at 0, 1 or 2 wks after transplanting. The growth of all species did not vary with compost age. Dianthus and pansy plants grown in 50% “New” compost and dianthus grown in 25% “New” compost produced significantly lower top dry mass than those grown in Sunshine Mix. All other treatments for cabbage, lettuce, dianthus and pansy showed no significant difference in top growth from those grown in Sunshine Mix. Poinsettia plants grown in Sunshine Mix produced greater total growth than all other treatments except those grown in equal portions of “New” compost, peat and perlite. Lettuce and cabbage leaf tissue contained cadmium levels less than 0.5 ppm regardless of compost level.


HortScience ◽  
2020 ◽  
Vol 55 (8) ◽  
pp. 1303-1307
Author(s):  
W. Garrett Owen

The objective of this study was to determine optimum fertilizer concentrations, identify leaf tissue nutrient sufficiency ranges by chronological age, and establish leaf tissue nutrient standards of containerized Russian sage (Perovskia sp.). Common Russian sage (P. atriplicifolia Benth.) and ‘Crazy Blue’ Russian sage were greenhouse-grown in a soilless substrate under one of six constant liquid fertilizer concentrations [50, 75, 100, 200, 300, or 400 mg·L−1 nitrogen (N)] with a constant level of a water-soluble micronutrient blend. Fertilizer concentrations sufficient for optimal plant growth and development were determined by analyzing plant height, diameter, growth index, primary shoot caliper, axillary shoot number, and total dry mass; they were found to be 100 to 200 mg·L−1 N after a 6-week crop cycle. Recently, mature leaf tissue samples were collected from plants fertilized with 100 to 200 mg·L−1 N and analyzed for elemental contents of 11 nutrients at 2, 4, and 6 weeks after transplant (WAT). An overall trend of increasing foliar nutrient concentrations over time was observed for all elemental nutrients. For instance, at 2 WAT, the total N concentrations of common Russian sage and ‘Crazy Blue’ Russian sage ranged between 3.68% and 5.10% and between 3.92% and 5.12%, respectively, and increased to ranges of 5.94% to 5.98% and 5.20% to 5.86% at 6 WAT, respectively. Before this study, no leaf tissue concentration standards have been reported; therefore, this study established leaf tissue concentration sufficiency ranges for the trialed Perovskia selections.


HortScience ◽  
2019 ◽  
Vol 54 (3) ◽  
pp. 463-469
Author(s):  
William Garrett Owen

Perennial hibiscus (Hibiscus sp.) are popular summer-flowering plants that are grown in greenhouses or nurseries, where growers must optimize production inputs such as fertility to maximize plant growth and produce high-quality flowering crops. The objective of this study was to determine the optimum fertilizer concentrations, identify leaf tissue nutrient sufficiency ranges by chronological age, and to expand leaf tissue nutrient standards of Hibiscus hybrid L. (hibiscus) grown in soilless substrates during container production. Two cultivars of hibiscus (H. hybrid L. ‘Mocha Moon’ and ‘Starry Starry Night’) were grown under one of six constant liquid fertilizer concentrations [50, 75, 100, 200, 300, or 400 mg·L−1 nitrogen (N)] with a constant level of water-soluble micronutrient blend in a greenhouse. The fertilizer concentrations sufficient for optimal plant growth and development were determined by analyzing plant height, diameter, growth index (GI), primary shoot caliper (PSC), and total dry mass, and they were found to be 100–300 mg·L−1 N after an 8-week crop cycle. Recently, mature leaf tissue samples were collected and analyzed for elemental content of 12 nutrients at 2, 4, 6, and 8 weeks after transplant (WAT) from plants fertilized with 100–300 mg·L−1 N. An overall trend of increasing sufficient tissue concentration over time was observed for total N, phosphorus (P), calcium (Ca), sulfur (S), zinc (Zn), copper (Cu), and boron (B), whereas a decreasing trend was observed for potassium (K), iron (Fe), manganese (Mn), and aluminum (Al). For instance, at 2 WAT, total N ranged from 3.1% to 5.1% N and increased to a range of 4.2% to 4.7% N at 8 WAT. At 2 WAT, Fe and Mn ranged from 79.2 to 103.6 mg·L−1 Fe and 66.3–82.8 mg·L−1 Mn and decreased to ranges of 75.6–82.9 mg·L−1 Fe and 18.1–99.7 mg·L−1 Mn at 8 WAT, respectively. Optimal leaf tissue concentration sufficiency ranges determined in this scientifically-based study were narrower than previously reported survey values for the genera Hibiscus.


2021 ◽  
Vol 1 (43) ◽  
pp. 21-21
Author(s):  
Alexander Saakian ◽  
◽  

The aim of the research was to evaluate the effectiveness of the effect of foliar fertilizing with microfertilizers on the yield and quality of oilseeds of spring rapeseed. The research was conducted in 2018-2019 under the conditions of the field experience of the Bunin Yelets State University. In crops of spring rape varieties Reef determined the effects of micronutrients Yara Vita Brassitrel and of a mixture of micronutrients Yara Vita Brassitrel and Yara Vita Bortrak. Yara Vita Brassitrel is a water-soluble fertilizer in the form of a powder, which includes Mg, S, B, Mn, Mo. Yara Vita Bortrak is a liquid fertilizer with a maximum concentration of boron. Non-root fertilizing with these fertilizers was carried out in the phases of 4 real leaves and stalking. There were no differences in the onset of the development phases of spring rapeseed depending on the use of microfertilizers Yara Vita Brassitrel and Yara Vita Bortrak. The positive effect of the studied microfertilizers on the biometric parameters of spring rapeseed was established. The use of the studied micronutrients contributed to the increase in activity clorofila clorofila a and b, carotenoids, the amount of pigments and leaf area assimilating the surface of plants of spring rape. Application of micronutrient fertilizers Yara Vita Brassitrel contributed to the growth of additional plant productivity 1,34 t/ha, a mixture of micronutrients Yara Vita Brassitrel + Yara Vita Bortrak 1,96 t/ha, with yields on the control option 16,25 kg/ha. Keywords: SPRING RAPESEED, MICROFERTILIZERS, YIELD, BIOMETRIC INDICATORS, NON-ROOT PROCESSING


2018 ◽  
Author(s):  
V.A. Borisov ◽  
A.M. Menshikh ◽  
V.S. Sosnov ◽  
G.F. Monakhos

Показано действие минеральных удобрений, микрокристаллического комплексного водорастворимого удобрения «Мастер» и органоминерального наноудобрения с ростостимулирующей активностью «Арксойл» при капельном орошении на урожайность и качество сладкого перца нового гибрида F1 Темп. Сочетание основного удобрения с листовой и корневой подкормками позволяет получить до 65 т/га плодов перца высокого качества.The action of mineral fertilizers, microcrystalline complex water soluble fertilizer Master and organic mineral nano-fertilizer with growth-stimulating activity Arksoil under drip irrigation on the productivity and quality of sweet pepper of the new hybrid F1 Temp is shown. The combination of basic fertilizer with leaf and root fertilizing allows to obtain up to 65 t/ha of pepper fruits of high quality.


Holzforschung ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Xiaodi Wang ◽  
Yongchao Zhang ◽  
Luyao Wang ◽  
Xiaoju Wang ◽  
Qingxi Hou ◽  
...  

AbstractAn efficient separation technology for hydrolysates towards a full valorization of bamboo is still a tough challenge, especially regarding the lignin and lignin-carbohydrate complexes (LCCs). The present study aimed to develop a facile approach using organic solvent extraction for efficiently fractionating the main components of bamboo hydrolysates. The high-purity lignin with only a trace of carbohydrates was first obtained by precipitation of the bamboo hydrolysate. The water-soluble lignin (WSL) fraction was extracted in organic solvent through a three-stage organic solvent extraction process, and the hemicellulosic sugars with increased purity were also collected. Furthermore, a thorough characterization including various NMR techniques (31P, 13C, and 2D-HSQC), GPC, and GC-MS was conducted to the obtained lignin-rich-fractions. It was found that the WSL fraction contained abundant functional groups and tremendous amount of LCC structures. As compared to native LCC of bamboo, the WSL fraction exhibited more typical LCC linkages, i.e. phenyl glycoside linkage, which is the main type of chemical linkage between lignin and carbohydrate in both LCC samples. The results demonstrate that organic phase extraction is a highly efficient protocol for the fractionation of hydrolysate and the isolation of LCC-rich streams possessing great potential applications.


Metabolites ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 357
Author(s):  
Muhammad Syamsu Rizaludin ◽  
Nejc Stopnisek ◽  
Jos M. Raaijmakers ◽  
Paolina Garbeva

Plants are faced with various biotic and abiotic stresses during their life cycle. To withstand these stresses, plants have evolved adaptive strategies including the production of a wide array of primary and secondary metabolites. Some of these metabolites can have direct defensive effects, while others act as chemical cues attracting beneficial (micro)organisms for protection. Similar to aboveground plant tissues, plant roots also appear to have evolved “a cry for help” response upon exposure to stress, leading to the recruitment of beneficial microorganisms to help minimize the damage caused by the stress. Furthermore, emerging evidence indicates that microbial recruitment to the plant roots is, at least in part, mediated by quantitative and/or qualitative changes in root exudate composition. Both volatile and water-soluble compounds have been implicated as important signals for the recruitment and activation of beneficial root-associated microbes. Here we provide an overview of our current understanding of belowground chemical communication, particularly how stressed plants shape its protective root microbiome.


Plant Disease ◽  
2021 ◽  
Author(s):  
Gardenia Orellana ◽  
Alexander V Karasev

Coleus scutellarioides (syn. Coleus blumei) is a widely grown evergreen ornamental plant valued for its highly decorative variegated leaves. Six viroids, named Coleus blumei viroid 1 to 6 (CbVd-1 to -6) have been identified in coleus plants in many countries of the world (Nie and Singh 2017), including Canada (Smith et al. 2018). However there have been no reports of Coleus blumei viroids occurring in the U.S.A. (Nie and Singh 2017). In April 2021, leaf tissue samples from 27 cultivars of C. blumei, one plant of each, were submitted to the University of Idaho laboratory from a commercial nursery located in Oregon to screen for the presence of viroids. The sampled plants were selected randomly and no symptoms were apparent in any of the samples. Total nucleic acids were extracted from each sample (Dellaporta et al. 1983) and used in reverse-transcription (RT)-PCR tests (Jiang et al. 2011) for the CbVd-1 and CbVd-5 with the universal primer pair CbVds-P1/P2, which amplifies the complete genome of all members in the genus Coleviroid (Jiang et al. 2011), and two additional primer pairs, CbVd1-F1/R1 and CbVd5-F1/R1, specific for CbVd-1 and CbVd-5, respectively (Smith et al. 2018). Five C. blumei plants (cvs Fire Mountain, Lovebird, Smokey Rose, Marrakesh, and Nutmeg) were positive for a coleviroid based on the observation of the single 250-nt band in the RT-PCR test with CbVds-P1/P2 primers. Two of these CbVd-1 positive plants (cvs Lovebird and Nutmeg) were also positive for CbVd-1 based on the presence of a single 150-nt band in the RT-PCR assay with CbVd1-F1/R1 primers. One plant (cv Jigsaw) was positive for CbVd-1, i.e. showing the 150-nt band in RT-PCR with CbVd1-F1/R1 primers, but did not show the ca. 250-bp band in RT-PCR with primers CbVds-P1/P2. None of the tested plants were positive for CbVd-5, either with the specific, or universal primers. All coleviroid- and CbVd-1-specific PCR products were sequenced directly using the Sanger methodology, and revealed whole genomes for five isolates of CbVd-1 from Oregon, U.S.A. The genomes of the five CbVd-1 isolates displayed 96.9-100% identity among each other and 96.0-100% identity to the CbVd-1 sequences available in GenBank. Because the sequences from cvs Lovebird, Marrakesh, and Nutmeg, were found 100% identical, one sequence was deposited in GenBank (MZ326145). Two other sequences, from cvs Fire Mountain and Smokey Rose, were deposited in the GenBank under accession numbers MZ326144 and MZ326146, respectively. To the best of our knowledge, this is the first report of CbVd-1 in the United States.


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