scholarly journals Evaluation of Antioxidants Supplementation on Renal, Hepatic and Cardiac Function Markers in Alloxan Induced Diabetes Mellitus Wister Rats

2020 ◽  
pp. 23-39
Author(s):  
Adline Erinma Ben-Chioma ◽  
Abiola Abubakar Sheudeen ◽  
Nwidum Leyiga Dornu ◽  
Ichebadu Isaac ◽  
Edna Ogechi Nwachuku ◽  
...  
Keyword(s):  
Vitamin E ◽  
Troponin I ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Cardiac Tissues ◽  
Diabetic Control Group ◽  
Group A ◽  
Group D ◽  
Control Group Group

Aim: To evaluate antioxidants supplementation (selenium and vitamin E) on renal, hepatic and cardiac function markers in alloxan induced diabetes mellitus in Wister rats. Study Design: Rats were randomly assigned into 5 groups with each group consisting of 5 rats. The treatment pattern involved the induction of hyperglycaemia in the rats followed by oral administration of selenium and vitamin E supplements singularly and in combination. The groups are as follow: Group A: Alloxan induced diabetic Rats treated with selenium (0.02 mg/kg) for 35 days. Group B: Alloxan induced diabetic Rats treated with Vitamin E (70.0 mg/kg) for 35 days. Group C: Alloxan induced diabetic Ratstreated with both Selenium and Vitamin E (0.02 mg/kg + 70.0 mg/kg) for a period of 35 days. Group D:  Alloxan induced diabetic Rats without any treatment (Positive control) for 35 days. Group E: Rats in this group were fed normally for 35 days without induction and treatment (Negative control). Place and Duration of the Study Area: The study was carried out in the Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nigeria over a period of 9 months (January, 2019 – September, 2019). Methodology: After the inducement of hyperglycaemia in the rats (Group A – D) with a single dose intraperitoneal (IP) injection of 140mg/kg body weight of alloxan hydrate, treatment with the antioxidants (selenium and Vitamin E) was performed for 35 days. At the end of 35 days, the animals were allowed to fast for 18 hours and sacrificed. Plasma specimen collected was used for the assay of Na+, K+, HCO3, urea, creatinine, cardiac troponin I, LDH, AST, ALT, ALP, bilirubin, protein, albumin globulin and MDA while renal, hepatic and cardiac tissues collected were used for histological investigations. Results: Significantly lower values were seen in Na+, K+, HCO3 and conjugated bilirubin in the diabetic rats without antioxidants supplementation (group D) when compared to diabetic rats with antioxidants supplementation of selenium and vitamin E (group A, B & C) and non-diabetic control group (group E). There were no significant differences seen when Group A, B, C and E were compared among one another. Also, significantly higher values were seen in AST, ALT, ALP, Unconjugated bilirubin, urea, creatinine, cardiac troponin I and MDA in the diabetic rats without antioxidants supplementation (group D) when compared with diabetic rats treated with antioxidants supplements of selenium and vitamin E (group A, B & C) and non-diabetic control group (group E). However, no significant differences were seen in LDH, total protein, albumin, globulin and total bilirubin at P=.05. Histological findings in the kidneys, liver and cardiac tissues of the rats treated with antioxidants supplement showed recovery tendencies compared to diabetic rats without antioxidant supplementation. Conclusion: Results obtained suggest that the use of selenium or vitamin E singularly or in combination has ameliorative effect on cardiac, renal and hepatic function markers in alloxan-induced diabetic rats. However, the combination of selenium and vitamin E had no synergistic advantage over the use of selenium or vitamin E alone. 

scholarly journals Protective effect of LIF-huMSCs on the retina of diabetic model rats

2021 ◽  
Vol 14 (10) ◽  
pp. 1508-1517
Author(s):  
Shan-Na Chen ◽  
◽  
Ying-Xue Ma ◽  
Song Chen ◽  
Guang-Hui He ◽  
...  
Keyword(s):  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Model ◽  
Group A ◽  
Retinal Structure ◽  
Hs Crp ◽  
Group B ◽  
And Function ◽  
Group D ◽  
Control Group Group

AIM: To investigate the protective effect of human umbilical cord mesenchymal stem cells (hUCMSCs) modified by the LIF gene on the retinal function of diabetic model rats and preliminarily explore the possible mechanism. METHODS: A stably transfected cell line of hUCMSCs overexpressing leukemia inhibitory factor (LIF) was constructed. Overexpression was verified by fluorescent quantitative polymerase chain reaction (qPCR). Forty-eight adult Sprague-Dawley rats were randomly divided into a normal control group (group A), streptozotocin-induced diabetic control group (group B), diabetic rats at 3mo injected with empty vector-transfected hUCMSCs (group C) or injected with LIF-hUCMSCs (group D). Four weeks after the intravitreal injection, analyses in all groups included retinal function using flash electroretinogram (F-ERG), retinal blood vessel examination of retinal flat mounts perfused with fluorescein isothiocyanate-dextran (FITC-dextran), and retinal structure examination of sections using hematoxylin and eosin staining. Expression levels of adiponectin (APN), high-sensitivity C-reactive protein (hs-CRP), and neurotrophin-4 (NT-4) in each group was detected using immunohistochemistry, PCR, Western blotting, and ELISA, respectively. RESULTS: A stable transgenic cell line of LIF-hUCMSCs was constructed. F-ERG and FITC-dextran examinations revealed no abnormalities of retinal structure and function in group A, severe damage of the retinal blood vessels and function in group B, and improved retinal structure and function in group C and especially group D. qPCR, ELISA, and Western blot analyses revealed progressively higher APN and NT-4 expression levels in groups B, C, and D than in group A. hs-CRP expression was significantly higher in group B than in groups A, C, and D, and was significantly higher in group C than in group D (P<0.05). CONCLUSION: LIF-hUCMSCs protect the retina of diabetic rats by upregulating APN and NT-4 expression and downregulating hs-CRP expression in the retina.

scholarly journals Comparative study of the effect of ethanolic extract of swietenia mahagoni seeds with rosiglitazone on experimentally induced diabetes mellitus in rats

2013 ◽  
Vol 39 (1) ◽  
pp. 6-10
Author(s):  
SMMMA Hasan ◽  
MI Khan ◽  
BU Kumar ◽  
MZ Sadeque
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Group B ◽  
Group D ◽  
Swietenia Mahagoni ◽  
Experimentally Induced

The study was performed to compare the blood glucose lowering effect of Swietenia mahagoni seeds with an oral antidiabetic drug, rosiglitazone in experimentally induced diabetic rats. Twentyfour healthy Long Evans Norwegian strain of rats were included in the study and divided into four groups (A, B, C and D) comprising 6 rats each. Group A (control group) received standard rat food for 14 days. Diabetes was induced by a single intraperitoneal administration of alloxan 120mg/kg body weight in Group B, C and D. Group B was given standard food for 10 days and considered as diabetic control. Group C and D were treated with ethanolic extract of Swietenia mahagoni seeds 1000mg/kg and rosiglitazone 10mg/kg orally respectively. Administration of ethanolic extract of Swietenia mahagoni seeds in group C and rosiglitazone in group D produced a significant reduction in blood glucose level as compared to diabetic control (group B). Histological examination of pancreas showed destruction of beta cells in Islets of pancreas in group B whereas retaining of islets and few degranulations of beta cells of pancreas found in group C and group D. The observations and results of the present study provide information that ethanolic extract of Swietenia mahagoni seeds has hypoglycaemic effect in experimentally induced diabetic rats which requires further investigation. DOI: http://dx.doi.org/10.3329/bmrcb.v39i1.15790 Bangladesh Med Res Counc Bull 2013; 39: 6-10

scholarly journals Effect of Fenugreek (Methi) on the Thymic volume in Streptozotocin-induced Diabetic Rats

1970 ◽  
Vol 16 (1) ◽  
pp. 1-4
Author(s):  
DK Mondal ◽  
MM Karim ◽  
S Mohal ◽  
BMA Yousuf ◽  
KM Shamim
Keyword(s):  
Body Weight ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Final Body Weight ◽  
Diabetic Control Group ◽  
Group A ◽  
Group B

Effect of fenugreek (Trigonella foenumgraecum) on thymic volume was studied in 30 long Evansrats. Among the total, 10 were treated with only vehicle (non-diabetic control - group A); 10 weretreated with streptozotocin only (diabetic control - group B) and 10 were treated with fenugreek(fenugreek treated - group C). Mean relative thymic volumes were 0.22210.018 ml/100 gm ofcorresponding final body weight (Mean 1 SEM), 0.14710.015 and 0.19910.020 in group A, B and Crespectively. In non-diabetic control group the thymic volume was significantly higher (p=0.005)than that of diabetic control group. In fenugreek treated diabetic group the volume was alsosignificantly higher (p=0.05) from that of diabetic control group and very much nearer to nondiabeticcontrol group (p=0.398). Fenugreek may preserve the thymic volume considerably indiabetic rats.doi: 10.3329/taj.v16i1.3892TAJ June 2003; Vol.16(1): 1-4

scholarly journals Comparative Study of the Antioxidant Effects of Metformin, Glibenclamide, and Repaglinide in Alloxan-Induced Diabetic Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Bonaventure Chukwunonso Obi ◽  
Theophine Chinwuba Okoye ◽  
Victor Eshu Okpashi ◽  
Christiana Nonye Igwe ◽  
Edwin Olisah Alumanah
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Complications ◽  
Significant Proportion ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Antioxidant Effects ◽  
And Oxidative Stress

Diabetes mellitus is one of the serious global health problems affecting a significant proportion of both developed and developing countries. Overproduction of free radicals and oxidative stress has been associated with the development of diabetic complications. In the present study, the antioxidant effects of metformin (MET), glibenclamide (GLI), and repaglinide (REP) were evaluated in alloxan-induced diabetic rats. The findings from this study may possibly help in understanding the efficacy of these standard drugs in managing the complications arising from diabetes mellitus (DM). Alloxan (130 mg/kg BW) was administered as a single dose to induce diabetes. Four (4) groups of rats (n=6) were used; group 1 served as diabetic control while groups 2, 3, and 4 were the diabetic test groups that received MET (25 mg/kg), GLI (2.5 mg/kg), and REP (0.5 mg/kg), respectively. The result of the study showed significant (p<0.05) improvement in the altered antioxidant enzymes (SOD, CAT) and GSH concentration in diabetic treated rats compared with the diabetic control group. MET and REP produced significant effect on the MDA concentration while GLI showed insignificant reduction in the MDA concentration compared with the diabetic control. Findings from this study suggest that the administration of MET, GLI, and REP exerts significant antioxidant effects in alloxan-induced diabetic rats, thus contributing to the protective effect against oxidative stress-induced damage during diabetic complications.

scholarly journals The Protective Effect of Marigold Hydroalcoholic Extract in STZ-Induced Diabetic Rats: Evaluation of Cardiac and Pancreatic Biomarkers in the Serum

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Esmaeel Ebrahimi ◽  
Saeed Shirali ◽  
Rahman Talaei
Keyword(s):  
Diabetes Mellitus ◽  
Protective Effect ◽  
Antioxidant Property ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Dependent Manner ◽  
Hydroalcoholic Extract ◽  
Diabetic Control Group ◽  
Male Wistar Rats

Diabetes mellitus is the most common endocrine disorder worldwide and it is usually along with complications such as retinopathy, neuropathy, nephropathy, and cardiovascular disease. The prevalence of diabetes is increasing and its treatment has created concerns in society. The use of herbal medicine can be helpful in the management of diabetes mellitus. The aim of this study was to investigate the protective effect of marigold hydroalcoholic extract under diabetes condition. A total of 36 male Wistar rats were randomly divided into four groups: normal control group, diabetic control group, and groups treated with 250 or 500 mg/kg hydroalcoholic extract of marigold flower during four weeks. At the end of the study, the rats were anesthetized with ketamine/xylazine, and sampling was performed through cardiac puncture. The results showed that treatment with marigold improved body weight. In addition, we determined that marigold normalized the level of CK-MB, total CK, amylase, and lipase in a dose-dependent manner. Probably these effects resulted from antioxidant property of marigold; thus we suggest that marigold flower can be useful for reduction of diabetes complication.

scholarly journals Preventive Role of Zingiber Officinale against Hyperglycemia in Alloxan Induced Diabetic Rats

2019 ◽  
Vol 30 (Number 1) ◽  
pp. 26-29
Author(s):  
S Sultana ◽  
N Y Mili ◽  
R Afroz ◽  
S Parveen
Keyword(s):  
Blood Glucose ◽  
Zingiber Officinale ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Group Iii ◽  
Diabetic Control Group ◽  
Glucose Estimation ◽  
Preventive Role

The experimental animal study was undertaken to investigate the preventive role ginger juice against hyperglycemia in alloxan induced diabetic rats.Male wistar rats,(130-150)gm wt fed on standard diet and water ad libitum, were divided into 3 groups(n=6) in each group: Group-L non-diabetic control group, Group-II, diabetic control & Group-III, normal rats pretreated with ginger before they were made diabetics. Diabetes was induced by inj. alloxan 150mg/kg body wt.,tp (Group-IL on 2nd day & Group-Ill, on the 9th day).Rats having blood glucose level of more than 7mmol/L on day 5(72 hours after alloxan inj) were considered diabetic & selected for experiment. Rats of Group-Ill received Zingiber officinale (ginger juice) (4m1/kg.body,wt orally) for 7 days (day 2-day8) through Pyles tube before alloxan induction & 3days after the induction. On day 12, animals were sacrificed under light ether anaesthesia, blood was collected by cardiac puncture for blood glucose estimation. Pretreatment with Zingiber officinale (ginger) juice significantly (p<0.01) reduced alloxan induced hyperglycemia.Zingiber officinale (ginger) is one of the most widely used spices and is reputed to have medicinal properties against diabetes mellitus. This study suggests that pretreatment with Zingiber officinale(ginger) prevents the development of hyperglycemia in alloxan induced diabetic rats.

scholarly journals Effect of Liposome Curcumin Combined with PDGF-BB on Tendon Healing in Rats

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Wang TL ◽  
◽  
Wu WJ ◽  
Gu JX ◽  
Hou XR ◽  
...  
Keyword(s):  
Tendon Healing ◽  
Tendon Injury ◽  
Control Group ◽  
Combined Application ◽  
Healthy Control ◽  
Group A ◽  
The Difference ◽  
Group B ◽  
Group D ◽  
Control Group Group

Objective: To study the effect of liposomal curcumin combined with PDGFBB on tendon healing after tendon injury in rats and explore its mechanism. Methods Thirty-six rats were randomly divided into four groups: healthy control group (group A), liposomal curcumin group (group B), PDGF-BB group (group C) and combined application group (group D). The rats in each group were divided into groups according to the experimental group. At the 4th and 8th weeks after operation, the general morphology, histomorphology, biomechanics and other aspects of rat tendon were detected and statistically analyzed. Results: The degree of tendon adhesion in group B and D was significantly less than that in group A and C, and the difference was statistically significant. The biomechanical test showed that the tendons in group C and D had higher strength and could withstand greater stress than those in group A and B. Conclusion: Liposome curcumin combined with PDGF-BB can significantly reduce the degree of adhesion after tendon injury, increase the strength of tendon healing, and significantly promote tendon healing.

scholarly journals Pharmacodynamics Drug Interactions of Metformin with Aspirin and Nifedipine

2016 ◽  
Vol 8 (1) ◽  
pp. 4 ◽  
Author(s):  
Khidir A. M. Hassan ◽  
Mahmoud M. E. Mudawi ◽  
Mansour I. Sulaiman
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Diabetic Control ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Citrate Buffer ◽  
Treatment Groups ◽  
Diabetic Control Group

Metformin is now being recognized as the standard therapy in T2D patients who are overweight. Metformin has many drug-disease interactions that can increase the risk of metformin-associated lactic acidosis. Therefore this study was conducted to evaluate any possible pharmacodynamic interactions between metformin and drugs used to treat chronic diseases e.g. Hypertension. The rats were fasted overnight before inducing diabetes with streptozotocin. The rats were given an intraperitoneal injection of streptozotocin (50 mg kg−1) freshly prepared in 0.1M sodium citrate buffer. The diabetic state was confirmed 72 h after streptozotocin injection. Diabetic rats were grouped into seven groups each group of five rats and distributed among the normal control group diabetic control group and the treatment groups. The treatment continued for 10 days. Blood samples were taken before treatment and after 10 days and analyzed for serum glucose, cholesterol, HDL, LDL, and triglycerides. In the diabetic control group which was given STZ alone the blood glucose level decreased significantly (p &lt; 0.05) after 10 days but still above the hyperglycemic level (200mg/dl). The same was observed in the group treated with metformin. The group treated with nifedipine and aspirin showed significant reduction (p &lt; 0.01) in the glucose level below the hyperglycemic level (200mg/dl). While the groups treated with (Metformin + Nifedipine) and (Metformin +Aspirin) showed highly significant reduction (P&lt;0.001) in blood glucose level. These results conclude that the combination of (metformin +Nifedipine) and the combination of (Metformin + Aspirin) have highly significant hypoglycemic effect. It also showed that Nifedipine has promising role in reducing blood glucose level, lipid profile especially LDL-cholesterol, and body weight.

scholarly journals THE EFFECTIVENESS OF ETHANOL EXTRACT OF BINAHONG LEAVES ON DIABETIC WOUND HEALING

2018 ◽  
Vol 11 (4) ◽  
Author(s):  
Devita Anggraeni ◽  
Claude Mona Airin ◽  
Slamet Raharjo
Keyword(s):  
Blood Glucose ◽  
Leukocyte Count ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Dorsal Skin ◽  
Group I ◽  
Diabetic Control Group ◽  
Chemical Profiles

This research aimed to study the effectiveness of ethanol extract of binahong leaves on blood glucose, insulin, blood chemical profiles (serum glutamic pyruvate transaminase=SGPT, serum glutamic oxaloacetic transaminase=SGOT, ureum, and creatinine), and skin histopathology in diabetic rat. A total of 20 male Wistar rats aged 3 months (± 250 gram) were divided into five groups, with four rats in each group. Group I (non-diabetic control) was injected with 0.1 M sodium citrate buffer, while group II (diabetic control), III, IV, and V were injected with single dose of Streptozotocin (STZ) at dose 40 mg/kg intraperitoneally (IP). One week after the injection, the dorsal skin of the rats were excised. Group I and II were given cream topically and 1% NaCMC orally, group III was given 50% ethanol extract of binahong leaves (EEB) topically and 1% NaCMC orally, group IV was given cream topically and EEB 300 mg/kg orally, and group V was given 50% EEB topically and EEB 300 mg/kg orally. These treatments were continued for 14 days. Blood samples were obtained at the end of study to examine blood glucose, insulin, and blood chemical profiles (SGOT, SGPT, ureum, and creatinine). Examination of skin histopathology and leukocyte count were also done. The result showed that blood glucose, insulin, SGOT, SGPT, and ureum level of diabetic rats given topical or oral EEB did not significantly different from diabetic control group, even though blood glucose, insulin, SGOT, SGPT, and ureum level of diabetic rats given topical and oral EEB were found lower compared to diabetic control group. Administration of EEB 300 mg/kg orally in diabetic rats could lower creatinine level significantly (P<0.05). Histopathological examination of dorsal skin of diabetic rats which were given EEB topically showed the decrease of fibroblast proliferation, leukocyte infiltration, and hemorrhage in dermis area. Leukocyte count on skin tissue was significantly lower (P<0.05) in diabetic rats given EEB. In conclusion, topical or oral administration of EEB can help healing process in diabetic wound.

scholarly journals PO-298 Effect of exercise and Siyeshen upon hippocampal cytochrome c and caspase-3 of diabetic rats

2018 ◽  
Vol 1 (5) ◽  
Author(s):  
Dongmei Wang ◽  
Jinming Zhang ◽  
Haibin Liu ◽  
Rongmei Wang
Keyword(s):  
Cytochrome C ◽  
Protein Expression ◽  
Caspase 3 ◽  
Diabetic Control ◽  
Exercise Group ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Mrna And Protein Expression ◽  
Cyt C

Objective To observe the effects of aerobic exercise and Siyeshen water extract on cytochrome c (Cyt c) and caspase-3 in hippocampus of diabetic rats and to explore the possible mechanism of improving diabetes. Methods Healthy male Wister rats fed with high fat and high sugar and combined with streptozotocin to establish type II diabetes model. They were randomly divided into 4 groups: diabetic control group, exercise group, Siyeshen group and exercise+Siyeshen group, and another normal control group, with 6 rats in each group. After aerobic exercise (15m/min, 5°slope, 60min, every other day) or/and Siyeshen (200mg/kg) gastrointestinal administration for 8w, the expression of Cyt c and caspase-3 in hippocampus of each group were detected by immunoblotting, and mRNA expressions were detected by RT-PCR. Results Compared with the normal control group, the mRNA and protein expressions of Cyt c and caspase-3 in the hippocampus of the diabetic control group were significantly increased (P<0.05). Compared with the diabetic control group, the blood glucose level of exercise group and exercise+ Siyeshen group decreased (P<0.05), the mRNA and protein expression of Cyt c and caspase-3 decreased significantly (P<0.05), but there were no significant changes in the mRNA and protein expression of Cyt c and caspase-3 between Siyeshen group and diabetic control group (P﹥0.05). Conclusions Exercise and exercise combined with Siyeshen can inhibit cytochrome c release and reduce caspase-3 protein expression, which may be related to the improvement of blood glucose levels in diabetic rats.

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