scholarly journals Antioxidant Properties and Effect of Ethanolic Extract of Pulcria crispa on Biochemical and Hematological Parameters of Albino Rats

2020 ◽  
pp. 14-22
Author(s):  
Haytham M. Daradka ◽  
Ramadan Al- Shdefat ◽  
Areej A. Eskandrani
Keyword(s):  
Antioxidant Activity ◽  
Hematological Parameters ◽  
Antioxidant Properties ◽  
Weight Ratio ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Behavioral Changes ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Different Doses

Background: Pulcria crispa (P. crispa) is an herbal plant traditionally used to treat common ailments. Objective: In this study, we investigated P. crispa for its phytochemical constituents, antioxidant properties and effects on biochemical and hematological parameters as well as safety in albino rats. Methods: Phytochemical analysis of ethanolic extract of P. crispa was conducted using standard procedures. In vitro 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay were used for the investigation of antioxidant activity of extract. Acute effects on physical and behavioral changes and mortality were monitored up to 72 h after administration of different doses of C. crispa  Chronic effects on body to organ ratio, biochemical and hematological parameters were measured after administration of rats with different doses of P. crispa extract for 30 days. Results: Alkaloids, flavonoids, phenols and tannins were the most abundant constituents found in P. crispa extract, which displayed a significant antioxidant activity measured by reducing power and DPPH assays. No physical, behavioral changes and mortality were noted following the acute treatment of rats with the extract. Similarly, no significant change in body to organ weight ratio was observed after chronic treatment. Hematological parameters including RBCs, Hb, PCV, MCV, MCH and MCHC values were unaltered while W.B.C count was elevated in P.crispa administered rats compared to control. crispa extract significantly reduced glucose, urea, creatinine, cholesterol, bilirubin, AST and ALT levels, whereas, triglycerides and total protein levels were increased in response to P.crispa treatment. Conclusions: This study demonstrates that P. crispa extract is rich in bioactive compounds and possesses significant antioxidant properties. Extract was also found to be safe and had no significant adverse effects on hematological parameters and exerted beneficial effects on biochemical parameters.

Antioxidant Properties of Ethanolic Extract from Ramulus mori (Sangzhi)

2009 ◽  
Vol 15 (5) ◽  
pp. 435-444 ◽  
Author(s):  
Zuofa Zhang ◽  
Jie Jin ◽  
Liangen Shi
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Experimental Models ◽  
Hplc Method ◽  
Total Phenolic ◽  
Dependent Manner

The antioxidant properties and total phenolic contents of four fractions of ethanolic extract from Ramulus mori were examined. Various experimental models including superoxide radical, hydroxyl radical, 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH) scavenging activity, metal chelating activity, and reducing power were used for characterization of their antioxidant activity. The four fractions showed various degrees of efficacy in each assay in a dose-dependent manner. The third fraction with the highest amount of total phenolics was the most potent antioxidant in all assays used. In addition, the most powerful compound (oxyresveratrol) was isolated and identified followed by on-line HPLC method and characterized by different spectral analysis. Oxyresveratrol exhibited impressive antioxidant activities in scavenging the superoxide radical, hydroxide radical, and DPPH. On the basis of the results obtained, Ramulus mori may serve as a potential source of natural antioxidant due to its significant antioxidant activity and oxyresveratrol may be the most powerful antioxidant in ethanolic extracts of Ramulus mori.

scholarly journals Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
M Miftahudin ◽  
Rini Hasibuan ◽  
Tatik Chikmawati
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Sod Activity ◽  
Different Doses

Abstract. Miftahudin, Hasibuan RS, Chikmawati T. 2019. Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia. Biodiversitas 20: 3715-3722. Three Selaginella species, S. ornata, S. plana, and S. willdenowii, from Java Island, Indonesia, have been known to have antioxidant properties; however, in vivo antioxidant activities of these species have not been reported. This research aimed to evaluate the in vivo antioxidant activity of ethanolic extract of three Selaginella species. The 70% ethanol extract of three Selaginella species at four different doses was administered to mice one day before being treated with oxidative stress. The liver tissue of mice treated with or without oxidative stress was analyzed their lipid peroxidation by measuring MDA concentration and Superoxide Dismutase (SOD) activities. The results showed that there were variations in antioxidant activity among the three Selaginella species. In general, the dose of 0.3 g extract kg-1 BW has been able to reduce lipid peroxidation and increase SOD activity. The administration of S. ornata extract to the mice at 1.2 g extract kg-1 BW reduced the MDA concentration to the lowest level, but the same dose of two other Selaginella extracts caused toxic effects in mice. The antioxidant activities of S. ornata and S. plana were better than that of S. willdenowii extract, and among those species, S. ornata has the best antioxidant activity.

scholarly journals PHYTOCHEMICAL PROFILING USING LC-Q-TOF-MS ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF A RARE SALT-SECRETING MANGROVE AEGIALITIS ROTUNDIFOLIA ROXB. LEAVES EXTRACT

2019 ◽  
pp. 37-47
Author(s):  
Debjit Ghosh ◽  
Sumanta Mondal ◽  
Prof. Karipeddi Ramakrishna
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
In Vitro Models ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Aegialitis Rotundifolia ◽  
Tof Ms

Objective: The present work deals with the qualitative study of the phytoconstituents present in Aegialitis rotundifolia Roxb., ethanolic leaves extract and evaluate its antioxidant properties in vitro. Methods: The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS). The antioxidant properties were investigated comprehensively using seven in vitro models viz., 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide (H2O2) scavenging, superoxide (SOD) radical scavenging, lipid peroxidation (LPO) assay, reducing power (RP), and total antioxidant activity. Results: The preliminary phytochemical analysis revealed the presence of several important phytochemical groups whereas the LC-Q-TOF-MS analysis detected 25 phytoconstituents in the extract mostly belonging to flavonoids and alkaloids. The test extract showed strong dose-dependent antioxidant activity in all the seven in vitro models, however, the activity of the extracts was slightly lower compared to the reference standard ascorbic acid. Conclusion: The test extract showed strong antioxidant properties which could be possibly due to the phytoconstituents detected in the extract.

scholarly journals Ethanolic Extract of Glycyrrhiza glabra to Ameliorate Oxidative Stress – Studies in vitro

2020 ◽  
Vol 2 (1) ◽  
pp. 80
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Glycyrrhiza Glabra ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Radical Scavenging Assay

Plants are major sources of bioactive organic molecules that are of interest to the pharmaceutical industry and are being screened for new drugs and chemicals. Glycyrrhiza glabra Linn. (Family: Fabaceae) also known as Liquorice, Mulaithi, or Yashtimadu is a well-known medicinal plant used in traditional medicine. Its roots and rhizomes are the medicinal parts used and are reported to possess antitumor, antimicrobial, antiviral, anti-inflammatory, immunoregulatory activities. This plant is also used as a flavoring agent due to its sweetness. In the present work, the ethanolic extract of Glycyrrhiza glabra was prepared, and its phytochemical analysis was done using HPLC. The in vitro antioxidant assays such as DPPH radical scavenging assay, Hydroxyl radical scavenging assay, total antioxidant activity assay, and total reducing power assay were done, and the results showed significant antioxidant activity of the extract. The extract was analyzed further to evaluate the ability to protect against oxidative stress in chicken liver tissue. The levels of glutathione and lipid peroxidation in H2O2 and/or G glabra extract-treated tissue indicated the potential to protect against oxidative stress under in vitro conditions. The in vitro comet assay results showed that the G glabra extract protected against H2O2 induced cellular DNA damage. These findings indicated promising antioxidant and antigenotoxic potential of G.glabra and need further exploration for translating these findings to its possible health benefits.

scholarly journals Antioxidant Activity, Enzyme Inhibition Potentials, and Phytochemical Profiling of Premna serratifolia L. Leaf Extracts

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Adelina Simamora ◽  
Adit W. Santoso ◽  
Kris H. Timotius ◽  
Ika Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Xanthine Oxidase ◽  
Metabolic Diseases ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Food Ingredient ◽  
Pbr322 Plasmid ◽  
Total Antioxidant

Premna serratifolia, commonly known as Arogo in Tentena-Sulawesi, is a popular vegetable. As a promising herbal tea and food ingredient, further investigation is required to find the best knowledge for medicinal use of P. serratifolia leaves. This research investigated the antioxidant activity of the ethanol (EEPS) and water (WEPS) extracts of P. serratifolia leaves, based on their scavenging activities on DPPH radicals and their reducing capacities (CuPRAC, total antioxidant/phosphomolybdenum, and ferric thiocyanate reducing power assays). The DNA-protecting effect by EEPS was tested using pBR322 plasmid DNA against •OH radical-induced damage. The inhibition potentials of both extracts against several enzymes related to metabolic diseases (α-glucosidase, α-amylase, xanthine oxidase, and protease) were evaluated. The phytochemical analysis was conducted by an LC-QTOF-MS/MS technique. EEPS proved to be a better antioxidant and had higher phenolic content compared to WEPS. EEPS demonstrated a protective effect on DNA with recovery percentage linearly correlated with EEPS concentrations. Strong inhibition on α-glucosidase and α-amylase was observed for EEPS; however, EEPS and WEPS showed weak inhibitions on xanthine oxidase and protease. LC-QTOF-MS/MS analysis identified seven main components in EEPS, namely scroside E, forsythoside A and forsythoside B, lavandulifolioside, diosmin, nobilin D, campneoside I, and isoacteoside. These components may be responsible for the observed enzymes inhibitions and antioxidant properties. Premna serratifolia leaves can be an appropriate choice for the development of nutraceutical and drug preparations.

scholarly journals Phenolic Profile and Antioxidant Activity of Propolis Extracts From Poland

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984977 ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Agnieszka Waśkiewicz ◽  
Tomasz Rogoziński ◽  
Izabela Ratajczak
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Natural Material ◽  
Antioxidant Power ◽  
Antioxidant Agents

Propolis is a natural material collected by honeybees, containing bioactive compounds that exhibit biological activity. The aim of this study was to assess the chemical composition of Polish propolis extracted with two different concentrations of ethanol, namely 70% and 96%, and to evaluate their antioxidant activity depending on extraction conditions. Samples of Polish propolis were extracted with 70% and 96% ethanol in order to obtain the ethanolic propolis extracts EEP70 and EEP96, respectively. Concentrations of 10 flavonoids and 6 phenolic acids were determined using the UPLC-PDA-TQD system. The antioxidant properties were determined based on the DPPH· free radical scavenging activity, Fe3+ reducing power assay, and ferrous ions (Fe2+) chelating activity assays. Moreover, the effects of the propolis extracts on human red blood cell morphology , the selective permeability of their membrane, as well as on free radicals-induced hemolysis were also assessed. Qualitative and quantitative analyses of both propolis extracts indicated that 70% ethanolic extract contained higher amounts of phenolic compounds than 96% ethanolic extract. The levels of antioxidant activity indicated that both Polish propolis extracts exhibited a high and comparable antioxidant power. The concentration of ethanol used for extraction had no effect on the antioxidant potential of propolis. The presented results indicate that the extracts of Polish propolis are rich in phenolic compounds and are very effective as antioxidant agents. Therefore, they may be applied as a constituent of products used in phytotherapy regardless of the concentration of ethanol used in propolis extraction.

scholarly journals Current Evaluation of the Tubiflora acaulis Kuntze (Acanthaceae): Pharmacognostical, Preliminary Phytochemical Investigation and Antioxidant Activity

2021 ◽  
pp. 89-106
Author(s):  
Pravin V. Gomase ◽  
Sunil Pawar
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Diagnostic Features ◽  
Standard Drug ◽  
Physical Evaluation ◽  
Phytochemical Investigation ◽  
Plant Collection

Aim: The present work was aimed at evolving with Pharmacognostical, Preliminary Phytochemical investigation and Antioxidant activity of plant Tubiflora acaulis Kuntze (Synonym Elytraria acaulis) belong to family Acanthaceae. Study Design: The permission was taken from Maharashtra State Biodiversity Board, Nagpur for plant collection, collected and authenticated and evaluated Pharmacognostical character including morphological and microscopical as well as Physical Evaluation, phytochemical process and antioxidant activity. Place and Duration of Study: The study was carried out at PSGVPMs College of Pharmacy, Shahada, Dist- Nandurbar (MS) Affiliated to KBCNMU, Jalgaon University, Jalgaon (MS), during 2019-21. Methodology: The plant material firstly taken permission from Maharashtra State Biodiversity Board, Nagpur for plant collection, collected and authenticated and evaluated Pharmacognostical character including morphological and microscopical as well as Physical Evaluation, extraction by successive solvent method, phytochemical process and antioxidant activity of Tubiflora acaulis Kuntze (Acanthaceae). Results: The macroscopical and microscopical studies revealed useful diagnostic features. Qualitative phytochemical analyses revels that Tubiflora acaulis Kuntze leaf separately for different extract like pet. Ether, chloroform and methanol and contained the secondary metabolites. The quantitative phytochemical analysis showed the presence of secondary metabolite. The extract of Tubiflora acaulis showed the antioxidant activity by DPPH, Nitric oxide and reducing power assay. Conclusion: The findings of the current research indicated that the ethanol leaf extract of Tubiflora acaulis Kuntze (Acanthaceae) had presence of secondary metabolites and potent antioxidant properties against standard drug.

scholarly journals Gastroprotective and antioxidant properties of ethanolic extract from pear fruit (Pyrus communis L.)

2020 ◽  
Vol 10 (5) ◽  
pp. 306-310
Author(s):  
Saliha Djidel ◽  
Amel Bouaziz ◽  
Assia Bentahar ◽  
Seddik Khennouf
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Pyrus Communis ◽  
Dry Extract ◽  
Pyrus Communis L ◽  
Protection Rate

The objective of this study was to evaluate the phenolic content, in vitro antioxidant activity and the gastroprotective effect of the ethanolic extract of pear fruits (Pyrus communis L.). The amount of polyphenols and flavonoids contents of ethanolic extract were found to be 77,50 ± 2,50 mg GAE/g of dry extract and 0,31 ± 0,003 mg QE/ g of dry extract, respectively. Whereas the amount of tannins was 61,43 ± 0,009 mg TAE/ g of dry extract. Hydroxyl radical scavenging and reducing power tests were used to evaluate the antioxidant activities of this extract. The scavenging effect of ethanolic extract against hydroxyl radicals showed IC50 value of 2.71 ± 0.02 mg/ ml. Reducing power test indicated the ability of ethanolic extract of pear fruits to reduce Fe+2 to Fe+3  with EC50value of 1.043±0.01 mg/ml. The treatment of rats with the ethanolic extract at  dose of 200 mg/kg reduced the ulcerogenic effect of ethanol with value of protection rate of 94.44%, but the protection rate decreased in 600 mg/kg treated group  to 90.17%.  In conclusion, natural antioxidants derived from pear fruits can prevent gastric ulcer through their antioxidant activity  Keywords: Pyrus communis L., Polyphenols, Antioxidant activity, Ulcer, Rat.

scholarly journals Therapeutic Response of Epimedium gandiflorum’s Different Doses to Restore the Antioxidant Potential and Reproductive Hormones in Male Albino Rats

Dose-Response ◽  
2020 ◽  
Vol 18 (3) ◽  
pp. 155932582095956 ◽  
Author(s):  
Naveed Munir ◽  
Zahed Mahmood ◽  
Muhammad Yameen ◽  
Ghulam Mustafa
Keyword(s):  
Therapeutic Response ◽  
Reducing Power ◽  
Reproductive Hormones ◽  
Male Rats ◽  
Albino Rats ◽  
High Dose ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Wide Range ◽  
Different Doses

Current study was planned to explore the therapeutic response of different doses of hydroethanolic extract of Epimedium grandiflorum leaves in male albino rats. Phytochemical analysis, HPLC and FTIR spectroscopy results revealed the presence of wide range of phenolic compounds and functional groups, respectively. Further, extract not induced significant hemolysis (7.56 ± 1.297%) against PBS (3.65 ± 0.35%) as negative control; while have significant clot lysis (44 ± 5.2%) potential, exhibited DPPH (78.87 ± 5.427%) scavenging, H2O2 (31.82 ± 3.491%) scavenging, antioxidant and reducing power activities. In vivo experimentation in albino male rats’ revealed that administration of different doses (50, 100, 200 mg/Kg b.w.) of extract orally for 42 days after CCl4 intoxication significantly (P < 0.05) restore the selected parameters including liver enzymes, renal profiles, and stress markers and significantly (P < 0.05) increased reproductive hormones like testosterone, luteinizing hormone, follicle stimulating hormone and prolactin while significantly (P < 0.05) decreased progesterone and estradiol toward normal in dose dependent manner. Significant (P < 0.05) improvement in the structural architecture of testicular tissue particularly in high dose group (200 mg/Kg b.w.) was also observed. Results revealed E. grandiflorum has significant therapeutic response to address the healthcare problems particularly of impotency.

scholarly journals Ethanolic Extract of Glycyrrhiza glabra to Ameliorate Oxidative Stress – Studies In vitro

2020 ◽  
Vol 10 (2) ◽  
pp. 2289-2297
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Glycyrrhiza Glabra ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Radical Scavenging Assay

Plants are major sources of bioactive organic molecules of interest for the pharmaceutical industry and are being screened for new drugs and chemicals. Glycyrrhiza glabra Linn. (Family: Fabaceae) is a very well-known medicinal plant traditionally used as medicine. Its roots and rhizomes are the medicinal parts used and are reported to possess antitumor, antimicrobial, antiviral, anti-inflammatory, immunoregulatory activities. The ethanolic extract of Glycyrrhiza glabra was prepared in the present work, and its phytochemical analysis and HPLC were done. The in vitro antioxidant activity assays such as DPPH radical scavenging assay, Hydroxyl radical scavenging assay, total antioxidant activity assay, and total reducing power assay was done, and the results showed significant antioxidant activity. The extract was analyzed further to evaluate the ability to protect against oxidative stress in chicken liver tissue. The extent of lipid peroxidation and glutathione in H2O2 and/or Glycyrrhiza glabra extract-treated tissue indicated the extract's potential to protect against oxidative stress under in vitro conditions. The in vitro comet assay results showed that the Glycyrrhiza glabra extract protected against H2O2 induced cellular DNA damage. These findings indicated promising antioxidant and antigenotoxic potential of Glycyrrhiza glabra and need further exploration for translating these findings to its possible health benefits.

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