Exploration of the Chemical Potential and Antioxidant Activity of Some Plants Used in the Treatment of Male Infertility in Southern Benin

Antioxidants are a family of substances that can neutralize free radicals and prevent and/or treat diseases associated with oxidative stress such as male’s infertility. Medicinal plants are one of the main sources of antioxidants. Aim: This work was aimed at evaluating the chemical and antioxidant potential of different extracts from some plants used in traditional Beninese medicine for the treatment of male infertility. Materials and Methods: The study was carried out on aqueous, hydro-ethanolic and ethanolic extracts from of the roots of Gardenia ternifolia (G. ternifolia), the whole plant of Cassytha filiformis (C. filiformis), the leaves of Rourea coccinea (R. coccinea) and the seed of Garcinia kola (G .kola). Quantification of the total polyphenols and flavonoids content of these extracts was evaluated respectively by the method using Folin Ciocalteu and the method using Aluminum trichloride. The antioxidant activity of the extracts was evaluated by molecular spectrophotometry using the free radical scavenging of DPPH and FRAP methods. Results: The results obtained indicated a variation of total polyphenols and flavonoids content according to the type of extract. Hydro-ethanolic extract of the various plants studied has a high polyphenols and flavonoids content. In variable proportions, all the extracts tested reduced the DPPH radical and ferric iron, reflecting their antioxidant potential. The best antioxidant activity has been obtained with the hydro-ethanolic extracts. Conclusion: This study showed that all the plant's extracts studied have antioxidant activity that varies with the type of extract. However, the hydro-ethanolic extractions showed the best antioxidant activities. The data obtained in the present study justified the use of these plants in management of pathologies involving oxidative stress.

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Mycochemical composition and antioxidant potential of Macrolepiota africana (Heim) Heinem. (Agaricaceae), an edible mushroom from Gabon

Open Access Research Journal of Life Sciences ◽

10.53022/oarjls.2021.2.1.0134 ◽

2021 ◽

Vol 2 (1) ◽

pp. 028-033

Author(s):

Hugues Calixte Eyi Ndong ◽

André Ledoux Njouonkou

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Activity Index ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Edible Mushroom ◽

Mushroom Species ◽

Chemical Screening ◽

Ethanolic Extracts ◽

Pharmacological Potential

Macrolepiota africana is a fungus used as food in Gabon for its culinary properties. However, its nutritional value, mycochemical contents, antioxidant properties and health potential still unknown. The present study investigated the phytochemicals and antioxidant properties of this mushroom species. This chemical screening was followed by a study of the antioxidant activity and a prediction of additional pharmacological activities of M. africana. Using standard methodology, the mycochemical analyses were carried out on aqueous, hydro-ethanolic and ethanolic fungi extracts. The antioxidant activity of the mushroom extracts was determined using DPPH radical scavenging assay. Apart from Digitoxigenine, flavonol and gitoxigenine that were not found in any of the extracts, all other tested mycochemical were found in atleast one of the extracts. Alkaloids, flavonoids, polyphenols, oses and holosides, proanthocyanidins and coumarins were found in all extracts at different intensity. Saponosids, sterols and triterpenes, tannins gallics, reducing sugar, anthracenosides and digitoxine were found in two extracts while tannins catechics, flavone and gitoxine were detected only in one extract. The dosage of phenolic compounds confirmed the richness of this fungus in total polyphenols, its moderate richness in proanthocyanidins, the lack of flavonoids in the aqueous extract and the moderate richness of the hydro-ethanolic and ethanolic extracts in flavonoids. Regarding the antioxidant activities, the results obtained for the DPPH trapping test showed that the different extracts had low to moderate antioxidant activity with antioxidant activity index (IAA) ranged 0.29 to 0.97 respectively in aqueous and ethanolic extracts. The presence of these mycochemical compounds along with the identified antioxidant activities shows that this M. africana have some pharmacological potential.

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Antioxidative, antimitotic, and DNA-damaging activities of Garcinia kola stem bark, Uvaria chamae root, and Olax subscorpioidea root used in the ethnotherapy of cancers

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2019-0073 ◽

2019 ◽

Vol 31 (3) ◽

Author(s):

Temidayo D. Popoola ◽

Olufunsho Awodele ◽

Folashayo Babawale ◽

Oluwatoyin Oguns ◽

Olawale Onabanjo ◽

...

Keyword(s):

Oxidative Stress ◽

Ascorbic Acid ◽

Cellular Proliferation ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Stem Bark ◽

Acid Pretreatment ◽

Garcinia Kola ◽

Traditional Remedies ◽

Scge Assay

AbstractGarcinia kola (GK) stem bark, Uvaria chamae (UC) root, and Olax subscorpioidea (OS) root are components of various indigenous/traditional anticancer regimens. It is, therefore, possible that they might combat oxidative stress and impair cellular proliferation linked to carcinogenesis. In this study, we investigated the antioxidative, mito-depressive, and DNA-damaging activities of the three plant extracts in order to provide further mechanistic insights into their potential anticancer roles in documented cancer remedies that include them. Antioxidative properties were investigated in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) radical scavenging assays and an animal model of drug (cisplatin)-induced oxidative stress. The Allium cepa assay and the single cell gel electrophoresis (SCGE) assay were used to assess mito-depressive and DNA-damaging activities. GK and OS showed significantly higher antioxidant activities in the DPPH assay than ascorbic acid; OS had the lowest IC50 of the three plants in the NO assay, comparable to that of ascorbic acid. Pretreatment with the extracts produced an ameliorative and protective effect against the cisplatin-induced oxidative stress as shown by inhibition of lipid peroxidation and improved or restored reduced glutathione and superoxide dismutase levels. In the Allium test, the three extracts produced significant decreases in root growth and also significant cytotoxicity as evidenced by decreased mitotic index. Each of the extracts also showed significantly increased tail DNA (%) in the SCGE assay, indicating the significant DNA-damaging effect. Taken together, this study demonstrates the possible chemopreventive and chemotherapeutic potentials of the three study extracts, which may explain the roles of their source plants in traditional remedies in the therapy of cancers.

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Antifungal and antioxidant activities of mature leaves of Myrcia splendens (Sw.) DC.

Brazilian Journal of Biology ◽

10.1590/1519-6984.179829 ◽

2019 ◽

Vol 79 (1) ◽

pp. 127-132

Author(s):

F. C. Pontes ◽

V. C. P. Abdalla ◽

M. Imatomi ◽

L. F. G. Fuentes ◽

S. C. J. Gualtieri

Keyword(s):

Antioxidant Activity ◽

Mycelial Growth ◽

Antioxidant Activities ◽

Pathogenic Fungus ◽

Radical Scavenging ◽

Inhibitory Effect ◽

Antioxidant Potential ◽

Economic Losses ◽

Mature Leaves ◽

Cosmetic Industry

Abstract In recent years, natural products with antifungal and antioxidant activities are being increasingly researched for a more sustainable alternative to the chemicals currently used for the same purpose. The plant pathogenic fungus Alternaria alternata is a causative agent of diseases in citrus, leading to huge economic losses. Antioxidants are important for the production of medicines for various diseases that may be related to the presence of free radicals, such as cancer, and in the cosmetic industry as an anti-aging agent and the food industry as preservatives. This study evaluated the antifungal and antioxidant potential of extracts of mature leaves of Myrcia splendens, a tree species that occurs in the Brazilian Cerrado. The antioxidant potential was analyzed by an assay of 1,1-diphenyl-2-picrylhydrazyl radical-scavenging method, and the antifungal activity was assessed through the evaluation of mycelial growth. Majority of the extracts exhibited a strong antioxidant activity, especially the acetonic extract (4A). The antioxidant activity may be related to the presence of phenolic compounds. However, the extracts showed no inhibitory activity of mycelial growth of the fungus tested, with the exception of dichloromethanic extract (2B), which had an inhibitory effect (10.2%) at the end of testing.

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Antioxidant activities of aqueous extracts from nine different rose cultivars

International Journal of Food Studies ◽

10.7455/ijfs.v7i2.484 ◽

2018 ◽

Vol 7 (2) ◽

Author(s):

Hamadia Khurshid ◽

Syed Mubashar Sabir ◽

Shahid Iqbal Awan ◽

Syed Rizwan Abbas ◽

Muhammad Irshad

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Liver Homogenate ◽

Antioxidant Potential ◽

Total Phenolic ◽

Aqueous Extracts ◽

Dpph Radical ◽

Rose Petals

Rose petals have been applied as food additives in teas, cakes and flavor extracts. The aim of this research study was to explore and reveal the antioxidant potential of aqueous extracts of rose petals belonging to nine genotypes of rose (wild as well as hybrid). The in vitro antioxidant activities of roses were studied by lipid peroxidation assay, DPPH radical scavenging assay, iron chelation assay, phosphomolybdenum reduction assay and total phenolic and flavonoid contents. The aqueous extract showed inhibition against lipid peroxidation (TBARS), induced by prooxidants (10 µM FeSO4) in mice liver homogenate. The free radical scavenging activities of the extracts were determined by scavenging of the DPPH radical. Extracts also showed metal chelating activities and high antioxidant activity in the phosphomolybdenum assay. The high content of phenolics and flavonoids detected in aqueous extracts may be responsible for the antioxidant activity. Amongst the different rose genotypes, screened, Rosa moschata (musk rose) was found to carry slightly higher antioxidant potential, owing to its higher phytochemical content.

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Etude phytochimique et activité antioxydante comparatives des trois variétés de Catharanthus roseus (L.) G. Don

International Journal of Biological and Chemical Sciences ◽

10.4314/ijbcs.v14i6.33 ◽

2020 ◽

Vol 14 (6) ◽

pp. 2352-2361

Author(s):

Hèzouwè Kagnou ◽

Oudjaniyobi Simalou ◽

Gneiny Whad Tchani ◽

Sabrina Sanvee ◽

Kokou Agbékonyi Agbodan ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Catharanthus Roseus ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Ethanol Extract ◽

Phytochemical Screening ◽

Phytochemical Study ◽

Hybrid Variety

Catharanthus roseus (L.) G. Don, est une plante médicinale largement utilisée en tradithérapie contre le diabète, l’hypertension, le cancer et les effets du stress oxydatif. Elle existe en trois variétés ("rosea", "alba" et "hybride") selon la couleur des fleurs. L’étude actuelle porte sur le criblage phytochimique et l’activité antioxydante in vitro (DPPH et FRAP) de l’extrait hydro-éthanolique des trois variétés. Les tests ont été effectués sur les extraits des feuilles, fleurs et racines. On a noté la présence des alcaloïdes, phénols, flavonoïdes, tanins, saponines, stérols, terpènes et quinines. Cependant, l’absence des sucres réducteurs, des flavonoïdes et des saponines dans les racines ainsi que les quinines dans les feuilles a été relevée. L'analyse comparative par piégeage du radical DPPH a montré que les extraits des fleurs et racines de l’hybride étaient plus actifs, respectivement de 130,023±3,2 et 131,189±1,8 mg EqQ/g. Une tendance différente a été observée dans les extraits des feuilles où la meilleure valeur DPPH de 65,746±2,5 mg EqQ/g est attribuée à "rosea" alors que celle du FRAP de 139,935±2,57 mg EqAA/g provient de "alba". Ces résultats devraient encourager la poursuite des travaux sur l'identification et la caractérisation de nouveaux composés bioactifs sélectivement abondant pour une meilleure valorisation de Catharanthus roseus sur le plan de phytomédicaments.Mots clés : Catharanthus roseus, variétés, criblage phytochimique, antioxydant. English Title: Phytochemical study and comparative antioxidant activity of the three varieties of Catharanthus roseus (L.) G. DonCatharanthus roseus (L.) G. Don, is a medicinal plant widely used in the treatment of diabetes, hypertension, cancer, and effects of oxidative stress. C. roseus exists in three varieties ("rosea", "alba" and "hybrid") depending on the colour of the flowers. This study focused on phytochemical screening and in vitro antioxidant activity (DPPH and FRAP) of the hydro-ethanol extract of the three varieties. The tests were carried out on leaves, flowers, and roots extracts. The presence of alkaloids, phenols, flavonoids, tannins, saponins, sterols, terpenes, and quinines were noted. However, the absence of reducing sugars, flavonoids, and saponins in the roots and quinines in the leaves was observed. Comparative analysis of DPPH radical scavenging showed that extracts from the flowers and roots of the hybrid were more active, respectively at 130.023±3.2 and 131.189±1.8 mg EqQ/g. FRAP values of the same samples were 175.166± 9.28 and 121.311±7.8 mg EqAA/g dry matter. For the leaves, the best DPPH value of 65.746±2.5 mg EqQ/g was attributed to "rosea" while the FRAP value of 139.935±2.57 mg EqAA/g was for "alba". These interesting antioxidant activities of the "hybrid" variety may be useful against oxidative stress pathologies. The current study might then promote the continuing identification and characterization of new bioactive compounds for a development of Catharanthus roseus.Keywords: Catharanthus roseus, varieties, phytochemical screening, antioxidant.

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Variation in Antioxidant Activity of Extracts of Acacia confusa of Different Ages

Natural Product Communications ◽

10.1177/1934578x1000500118 ◽

2010 ◽

Vol 5 (1) ◽

pp. 1934578X1000500

Author(s):

Yu-Tang Tung ◽

Shang-Tzen Chang

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Scavenging Activity ◽

Acacia Confusa ◽

Ethanolic Extracts ◽

Different Ages ◽

Branch Wood

The antioxidant activity of extracts of Acacia confusa of different ages has been evaluated. Ethanolic extracts of bark, twigs, branches (2- and 5 cm-diameter branch-bark, 2- and 5 cm-diameter branch-wood), and leaves of 15-, 25-, and 35-year-old A. confusa were examined. Results indicate that the ethanolic extracts of 5 cm-diameter branch-bark and bark of A. confusa exhibited stronger antioxidant activities, and higher phenolic and proanthocyanidin contents than the other plant parts. Furthermore, twig, 2 cm-diameter branch-wood, 2 cm-diameter branch-bark, and 5 cm-diameter branch-wood extracts of 35-year-old specimens showed better antioxidant activities and higher phenolic contents, followed by the 25- and 15-year-old specimens tested. This reveals that the composition of extracts and their differential antioxidant activities are dependent on the age of A. confusa. In addition, the total phenolic content of A. confusa ethanolic extracts has a high positive correlation with the DPPH free-radical scavenging activity (R2 = 0.83), superoxide radical-scavenging activity (R2 = 0.66), ferrous ion-chelating effect (R2 = 0.67), and reducing power (R2 = 0.92).

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Antioxidant Activities of Stilbenoids fromRheum emodiWall

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/603678 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 11

Author(s):

Yuan-yuan Chai ◽

Fang Wang ◽

Yan-li Li ◽

Ke Liu ◽

Hui Xu

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Inflammatory Diseases ◽

Radical Scavenging ◽

Reducing Power ◽

Antioxidant Potential ◽

Hydroxyl Group ◽

Antioxidant Power

Rheum emodiWall has been reported to possess protective effect in many inflammatory diseases and oxidative stress-related injuries. This study aims to investigate antioxidant power of stilbenoids fromR. emodiand then explore the material basis for its antioxidant potential. The most abundant stilbenoid piceatannol-4′-O-β-D-glucopyranoside (PICG) and its aglycon piceatannol (PICE) were isolated fromR. emodirhizome. Using well-accepted antioxidant chemicals as reference, antioxidant activity of these stilbenoids was examined by measuring DPPH and superoxide anion radical scavenging, ferric reducing power, and inhibition of lipid peroxidationin vitro. Both PICG and PICE displayed promising antioxidant activity in all the four assays. Comparisons among the tested compounds indicated that PICE has the most potent antioxidant activity and the presence of 3′-hydroxyl group may enhance antioxidant activity of stilbenoids. The antioxidative effect of PICE at the cellular level was further demonstrated on the model of hydrogen-peroxide-induced H9c2 rat cardiomyoblasts injury. Taking into account the rapidin vivometabolic transformation of PICG into PICE it can be inferred that the most abundant stilbenoid PICG may be an important constituent responsible for the antioxidant potential ofR. emodiand promising to be developed as an antioxidant agent for supplementary or therapeutic use.

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ANTIOXIDANT ACTIVITY OF ALKALOID FRACTIONS OF ZANTHOXYLUM ACANTHOPODIUM DC. FRUITS WITH 1,1-DIPHENYL-2-PICRYLHYDRAZYL ASSAY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11s1.26560 ◽

2018 ◽

Vol 11 (13) ◽

pp. 33

Author(s):

Rosidah Rosidah ◽

Poppy Anjelisa Zaitun Hasibuan ◽

Ginda Haro ◽

Puteri Masri ◽

Denny Satria

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Free Radical ◽

Antioxidant Activities ◽

Ethanol Extract ◽

Liquid Extraction ◽

The Body ◽

Antioxidant Potential ◽

Liquid Liquid Extraction

Objective: Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radical-induced oxidative stress. The purpose of this study is to determine antioxidant activities of alkaloid fractions of Zanthoxylum acanthopodium DC. fruits.Methods: Z. acanthopodium DC. fruit was extracted by maceration. Ethanol extract was fractionated with liquid-liquid extraction using n-hexane and chloroform at pH 3, 7, 9, and 11 to obtained alkaloid fractions. Antioxidant activity for extract and fractions was determined with 1,1-diphenyl-2- picrylhydrazyl.Results: The IC50 of extract and fractions was 101.11±0.11, 273.24±0.12, 116.62±0.04, 61.12±0.01, 23.15±0.01, and 112.39±0.04 μg/mL, respectively.Conclusion: The results reveal that alkaloid fractions of Z. acanthopodium DC. fruit have very strong antioxidant potential. Our further study is to isolate the alkaloid compounds.

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Physiochemical properties and antioxidant potential of Persea Americana seed oil

10.31221/osf.io/jtf4s ◽

2019 ◽

Author(s):

Chem Int

Keyword(s):

Seed Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Acid Value ◽

Persea Americana ◽

Antioxidant Potential ◽

Physiochemical Properties ◽

Physiochemical Parameters ◽

Ic50 Value

Oil extracted from Persea Americana seed was assayed for its physiochemical properties and antioxidant potential using various standard methods. The oil content of the seed was found to be < 10%. Brownish-red color oil was liquid at room temperature, with specific gravity of 0.91±0.02 g/mL. Other physiochemical parameters determined were; acid value (4.51±0.08 mgKOH/g), %FFA (2.26±0.08), peroxide value (2.40±0.57 mgO2/Kg), ester value (31.26±0.03 mgKOH/g), saponification value (35.76±0.07 mgKOH/g) and iodine value (23.5±0.07). The results of the antioxidant activities of the seed oil showed that the flavonoid content (80.00±1.41 mgQE/g) was ~10 folds higher than the phenolic content (8.27±0.06 mgGAE/g). The DPPH radical scavenging value was found to be 51.54±0.25% with an IC50 value of 4.68±0.02 mg/mL and reducing power with an average absorbance of 0.85±0.01 and an IC50 value of 0.001±0.02 mg/mL. Gallic acid showed better antioxidant activities than the oil studied. The results obtained in this study showed that Persea Americana seed oil has nutritional, industrial as well as medicinal potentials.

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Antioxidant activity of cookiessupplemented with sugarbeet dietary fiber

Sugar Industry ◽

10.36961/si11177 ◽

2011 ◽

pp. 151-157 ◽

Cited By ~ 2

Author(s):

Marijana B. Saka ◽

Julianna F. Gyura ◽

Aleksandra Mišan ◽

Zita I. Šereš ◽

Biljana S. Pajin ◽

...

Keyword(s):

Antioxidant Activity ◽

Shelf Life ◽

Dietary Fiber ◽

Wheat Flour ◽

Room Temperature ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Functional Characteristics

The antioxidant activity of cookies prepared by the addition of sugarbeet dietary fibers was investigated in order to estimate their influence on functional characteristics and shelf-life of cookies. Treated fiber (TF) was obtained from sugarbeet by extraction with sulfurous acid (75 °C at pH = 5.7during 60 min) and treatment with hydrogen peroxide (20 g/LH2O2 at pH = 11 during 24 h). The fiber obtained was dried (80 °C), ground and sieved. TF was investigated in comparison with commercially available Fibrex®. The cookies were prepared by the addition of 0, 7, 9 and 11% of sugarbeet dietary fiber as a substitute for wheat flour in the formulation of cookies. The antioxidant properties of cookies were tested every 7 days using a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity test during 6 weeks of storage at room temperature (23 ± 1 ºC). The obtained results indicated that substitution of wheat flour with Fibrex® in the formulation of cookies upgraded the antioxidant activity, i.e. the functional characteristics of Fibrex®-enriched cookies and could prolong their shelf-life. In contrast, TF did not increase the antioxidant activity of TF-enriched cookies. The better antioxidant activities of Fibrex®-enriched cookies could be attributed to the presence of ferulic acid.

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