scholarly journals Antidiabetic Effect of Katakakhadiradi kashayam by Improving the Insulin Expression and Glucose Metabolising Enzyme

2021 ◽  
pp. 1-8
Author(s):  
Angelie Jessica Subbiah ◽  
M. Kavimani ◽  
Mukilan Ramadoss ◽  
Mudiganti Ram Krishna Rao ◽  
K. Prabhu
Keyword(s):  
Side Effects ◽  
Wistar Rats ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Β Cells ◽  
Metabolizing Enzymes ◽  
Antihyperglycemic Activity ◽  
Antidiabetic Effect ◽  
Pathological Conditions ◽  
Insulin Expression

Many plants provide a rich source of bioactive chemicals, which are free from undesirable side effects and possess powerful pharmacological actions. The present study was carried out to find the antidiabetic effect of   Katakakhadiradi kashayam (KKK) by improving the insulin expression and regulating properly the glucose metabolising enzymes. The diabetes was induced in combination with streptozotocin and nicotinamide injection to Wistar rats. Diabetic rats were treated with   Katakakhadiradi kashayam orally at doses of 100, 200 and 300 mg/kg/bw for 28 days, and the obtained results of parameters were compared with glibenclamide. The antidiabetic effect of Kashayam was measured by the expression of insulin by immunohistochemistry and restoring the normal clinical values of glucose metabolizing enzymes. The present study specified that hyperglycemia leads to pathological conditions in pancreatic tissue with decreased expression of insulin in β-cells whereas the   Katakakhadiradi kashayam normalised the production of insulin. The study found that the antihyperglycemic activity of   Katakakhadiradi kashayam L. is mainly due to their ability to restore the function of pancreatic tissues by causing an increase in insulin output and maintaining the glucose metabolising enzymes.

scholarly journals Study Antidiabetic Effect of Momordica Charantia (bitter gourd) seeds on Alloxan Induced Diabetic Rats

2010 ◽  
Vol 34 (1) ◽  
pp. 165-170
Author(s):  
Auroba M.S. Ibrahem
Keyword(s):  
Oral Administration ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Treated Group ◽  
Progressive Increase ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Momordica Charantia ◽  
Antidiabetic Effect

The aim of the present study is to investigate the antidiabetic effect of the aqueous extract of Momordica charantia seeds in alloxan - induced diabetic rats. Forty male albino Wistar rats were used, divided randomly into four groups (10 each). Oral administration of the seed extract at a dose of 150 mg/kg B.W. for 30 days showed a significant decrease in fasting blood glucose. Our results were greatly lower after oral administration of aqueous extract of Momordica charantia seeds in alloxan- induced diabetic groups than glibenclamide treated group. Also Momordica charantia extract and glibenclamide administered rats showed progressive increase in body weight

scholarly journals Antidiabetic Effect ofMonolluma quadrangulaIs MediatedviaModulation of Glucose Metabolizing Enzymes, Antioxidant Defenses, and Adiponectin in Type 2 Diabetic Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
May N. Bin-Jumah
Keyword(s):  
Insulin Sensitivity ◽  
Glucose Tolerance ◽  
Liver Lipid ◽  
Diabetic Rats ◽  
Antioxidant Defenses ◽  
Metabolic Disturbances ◽  
Metabolizing Enzymes ◽  
Antidiabetic Effect ◽  
Type 2 Diabetic

Monolluma quadrangulais a succulent bush traditionally used to treat diabetes and peptic ulcer. The present study aimed to investigate the effect ofM. quadrangulahydroethanolic extract on glucose tolerance, insulin sensitivity, glucose metabolizing enzymes, lipid profile, and adiponectin expression in type 2 diabetic rats. In addition, the study evaluated the antioxidant and anti-inflammatory activities of theM. quadrangulaextract. Type 2 diabetes was induced by feeding rats a high-fat diet (HFD) for 8 weeks followed by 30 mg/kg streptozotocin (STZ). Diabetic rats received 300 or 600 mg/kgM. quadrangulaextract for 4 weeks. HFD/STZ diabetic rats showed impaired glucose tolerance, reduced insulin secretion, and insulin resistance. HFD and STZ induced a significant increase in serum cholesterol, triglycerides and proinflammatory cytokines, and liver lipid peroxidation. Treatment withM. quadrangulaextract ameliorated these metabolic disturbances and increased liver glycogen, hexokinase activity, and antioxidants.M. quadranguladeclined the activity of liver glucose-6-phosphatase and fructose-1,6-biphosphatase. In addition,M. quadrangulaextract increased serum adiponectin levels and hepatic adiponectin expression in HFD/STZ diabetic rats. In conclusion,M. quadrangulaexerts antidiabetic effect mediatedviaameliorating glucose tolerance, insulin sensitivity, glucose metabolizing enzymes, and antioxidant defenses. Increased adiponectin levels and expression seems to mediate, at least in part, the antidiabetic effect ofM. quadrangula.

scholarly journals Hypoglycemic Activities of Ethanolic Leave Extract of Acalypha Wilkesiana in Streptozotocin-Induced Diabetic Wistar Rats

2021 ◽  
pp. 126-132
Author(s):  
Iyamu A. O. ◽  
Otamere H. O. ◽  
Akpamu U. ◽  
Nwadike I. ◽  
Njoku O. U. ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Alternative Therapy ◽  
Diabetic Rats ◽  
Dependent Manner ◽  
Insulin Deficiency ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Acalypha Wilkesiana ◽  
Stimulation Of

Diabetes is a rampant metabolic disorder of insulin deficiency or resistance. In support of the alternative therapy quest, this study investigates the antidiabetic actions of ethanolic leave extract of Acalypha wilkesiana (A. wilkesiana) in diabetic rats. The study was conducted in 3 phases using streptozotocin (50mg/kg) induced diabetic adult Wistar rats. In phase one, 18 diabetic rats were divided into 3 groups (n=6) and treated with distilled-water (10ml/kg), glimepiride (0.1mg/kg) and ethanolic leave extract of A. wilkesiana (250mg/kg) respectively. On separate 18 diabetic rats (phase two), 5% glucose (10ml/kg) was administered after treatments as in phase one. Blood glucose was measured at 0 and 30-minute intervals for 180 minutes in both phases. On another 18 diabetic rats (phase three), similar treatments were given daily for 14 days. Blood glucose was measured at day 0, 3 days after induction, 3, 7, 10, and 14 days treatments. ANOVA was carried out with p <0.05 as significant. The results showed progressively hypoglycemic actions significant from the 90th minute with glimepiride (285.17±12.09mg/dl) and the 120th minute with the extract (279.83±14.88mg/dl) through 180 minutes compared to control in 1st-phase. There was a significant obliterating effect on glucose-induced hyperglycemia in a time-dependent manner at 90th through 180th minutes after glucose loading in glimepiride and extract-treated groups compared to control (2nd phase). Streptozotocin-induced decreased body weight was improved in glimepiride and extract-treated groups by days 7 and 14 and there was a significant steady duration-dependent decrease in blood glucose from the 3rd to 14th day of treatments compared to control. The findings suggest that ethanolic leaves extract of A. wilkesiana possesses antidiabetic action probably through stimulation of pancreatic β-cells or improves insulin action.

Antihyperglycemic activity of Ficus carica leaves extracts on Streptozotocin induced diabetic rats

2021 ◽  
pp. 4151-4156
Author(s):  
Tathagata Roy ◽  
Susanta Paul ◽  
Victor Roy Chowdhury ◽  
Arijit Das ◽  
Srikanta Chandra ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Wistar Rats ◽  
Diabetic Rats ◽  
Ficus Carica ◽  
Citrate Buffer ◽  
Antihyperglycemic Activity ◽  
Treatment Of Diabetes

Antihyperglycemic activity of leave extracts of Ficus carica was evaluated on STZ induced diabetic rats. Diabetes was induced in albino Wistar rats of either sex by intraperitoneal (60mg/kg b.w.) of STZ, freshly dissolved in citrate buffer (0.01 M, pH 4.5). Ficus carica leave extract in different solution (viz. petroleum ether, ethyloacetate, methanol and aqueous) were administered to diabetic rats for 9 days. The effect of extracts on blood glucose and body weight was studies on day 1st and 9th. The study showed that the ethyl acetate, methanolic and aqueous extract of Ficus sarmentosa leaves reduced blood glucose level and body weight significantly. This may justify the use of ficus species as ethanomedical medicine for treatment of diabetes mellitus.

scholarly journals Pancreato-protective PANCREATOPROTECTIVE EFFECT OF SILYMARIN ON OXIDATIVE STRESS IN ALLOXAN-INDUCED HYPERGLYCEMIA IN MALE WISTAR RATS

2019 ◽  
pp. 121-125
Author(s):  
UMA NARAYANAMURTHY ◽  
SYLVIA SANTHAKUMARI A ◽  
NIRMALA P
Keyword(s):  
Lipid Peroxidation ◽  
Diabetic Complications ◽  
Wistar Rats ◽  
Serum Glucose ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Group Iv ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats

Objective: The objective of the study was to study the silymarin’s pancreatoprotective effect in alloxan-induced Type I diabetes mellitus. Numerous studies have evidence to prove the fact that antioxidant defense mechanism of flavonoids has overcome the progression of chronic diabetic complications. Methods: A total of 24 male Wistar rats were divided into four groups (n=6): Group I normal control, Group II, Group III, and Group IV were induced diabetes with alloxan. Group I and Group II diabetic rats received the vehicle (PO). Group III was treated with silymarin 400 mg/kg (PO). Group IV was treated with glibenclamide 0.5 mg/kg, per orally for 21 days. Fasting blood samples were collected from all four groups of animals at the end of 21 days to evaluate serum glucose and glycosylated hemoglobin (HbA1c). Pancreatic tissue extraction, to perform lipid peroxidation and histopathological study confirms the level of oxidative damage to tissues and recovery after treatment. Results: The serum glucose and HbA1c levels significantly increased in untreated diabetic rats, also a significant rise in lipid peroxidation and necrosis of beta cells in the pancreatic tissue. The rise in serum glucose levels was ameliorated in rats treated with silymarin, pancreatic tissue showed increased antioxidant levels, decreased lipid peroxides, and minimal changes and signs of regeneration of beta cells. Conclusion: This study adds experimental evidence to the fact that silymarin is an effective nutritional supplement to treasure pancreatic beta-cell reserve and to delay diabetic complications.

scholarly journals Improving the Reliability and Utility of Streptozotocin-Induced Rat Diabetic Model

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Yanlin Wang-Fischer ◽  
Tina Garyantes
Keyword(s):  
Side Effects ◽  
Sodium Channel ◽  
Pancreatic Islets ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Partial Recovery ◽  
Sprague Dawley ◽  
Area Reduction ◽  
Diabetic Model ◽  
Pancreatic Toxicity

The Streptozotocin- (STZ-) induced diabetic model is widely used; however, unexplained acute toxicity has given the model an unreliable reputation. To improve the reliability and utility of this model, we characterize the age dependence of STZ toxicity and introduce novel endpoints to assess diabetic complications and reveal possible mechanisms for diabetic development. Diabetes was induced by STZ injection into male, 6 to 23 weeks old, Sprague-Dawley rats. Their metabolic (glucose, lipids, and hormones), inflammatory (cytokines), histologic and behavioral endpoints were observed for 1.2 years. Analgesic compounds were assessed for efficacy treating neuropathy. Acute mortality, within a week of STZ injection (50–65 mg/kg i.v.), was inversely correlated to animal age. Only 3% of rats, age 6–11 weeks, died in the week following STZ injection, whereas 83% of rats 12 to 17 weeks old and 91% of rats 18 weeks or older died in the same week. Partial model recovery (normalized insulin, glucose and food/water intake) was observed starting at week 36; however, pain scores, kidney enlargement, and cataract formation continued to show progression consistent with the diabetic state. Unique noninvasive observational measurements, such as haircoat quality and diarrhea scores, served as useful endpoints for this model. The increased plasma cytokines (such as TNF-α, IL-4, and IL-6) and inflammatory cell infiltration into the pancreatic islets are strong evidence of inflammation in the STZ-induced diabetic model. Pancreatic tissue staining revealed total islet area reduction and confirmed STZ-specific pancreatic toxicity; however, the β-cell density per area in pancreatic islets and insulin levels statistically increased over time in the diabetic rats, suggesting a mechanism for partial recovery of diabetic symptoms. Voltage-gated sodium channel (NaV1.7 specific, peripherally restricted) blocker, CC4148, inhibited neuropathy without side effects as compared to a nonspecific sodium channel inhibitor, Mexiletine, or GABA analog, Pregabalin, which inhibited neuropathy with side effects.

scholarly journals GLP 1 Regulated Intestinal Cell’s Insulin Expression and Selfadaptation before the Onset of Type 2 Diabetes

2019 ◽  
Vol 9 (2) ◽  
pp. 325-330
Author(s):  
Shivani Srivastava ◽  
Harsh Pandey ◽  
Surya Kumar Singh ◽  
Yamini Bhusan Tripathi
Keyword(s):  
Diabetic Control ◽  
Diabetic Rats ◽  
Intestinal Cells ◽  
Time Interval ◽  
Β Cells ◽  
Dipeptidyl Peptidase 4 ◽  
Insulin Expression ◽  
Dpp Iv ◽  
Glucagon Like Peptide 1 ◽  
High Level

Purpose: Basically insulin is known to be secreted by β cells of the pancreas. Recently, it has also been found to be produced and expressed by intestinal epithelial cells with the help of L cells secreting glucagon like peptide 1 (GLP 1). Here, we have studied the same intestinal insulin expression property in T2D rats. Methods: Following 2 weeks of high fat diet (HFD) consumption, we have been given a single dose of streptozotocin (STZ) (35 mg/kg bw). Rats were then sacrificed after 1, 7 and 21 days. The GLP 1 analogue, liraglutide was also given to one group of diabetic rats, upto their respective durations. Intestinal cells apoptosis were checked by tunnel assay, Incretin hormones secretion and dipeptidyl peptidase 4 (DPP-IV) activity were analyzed through ELISA and immunohistochemistry was used to determine the insulin expression of intestine at different time interval during diabetes progression. Results: As compared to 1 and 21 days, we have found minor cells apoptosis in 7 days group along with high level of GLP 1 in diabetic model. Further, these effects were enhanced by liraglutide. In response to these we have found, decreased insulin expression after 21 days and with no significant effect upto 7 days in diabetic control groups. In contrast to this, GLP-1 level and insulin expression enhances prominently after 7 days of liraglutide treatment. Conclusion: These results explain the self-adapting approach of intestinal cells against diabetes onset and insulin expression enhancing property of liraglutide under stressful conditions. This study should be continued in future for the development of intestinal insulin producing drugs, to control diabetes under irreversible β cells damage.

Antidiabetic effect of Psychotria malayana Jack in induced type 1 diabetic rat

MEDISAINS ◽  
2020 ◽  
Vol 18 (1) ◽  
pp. 19
Author(s):  
Fairuz Fairuz ◽  
Hasna Dewi ◽  
Humaryanto Humaryanto
Keyword(s):  
Blood Glucose ◽  
Side Effects ◽  
Type I Diabetes ◽  
Langerhans Cell ◽  
Diabetic Rat ◽  
Type I ◽  
Antidiabetic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Therapies for hyperglycemic treatment, including insulin and oral diabetes medications, have been confirmed to cause several side effects. Thus, finding new drugs with fewer side effects is of high importance. Salung leaf herb (Psychotria malayana Jack) reported used in traditional societies as a treatment for diabetes. However, the scientific proof of this plant for diabetes treatment is still lacking.Objective: To evaluate the antidiabetic effect of the P. malayana jack in induced type 1 diabetic rats by assessing blood glucose level and pancreatic cells in white rats.Methods: Alloxan used to induce type I diabetes. Rats randomly divided into six groups. A Group P1 received 250 mg/kg BW; group P2 received 500 mg/kg BW, group P3 received 1000 mg/kg BW. While group 4 basal received no treatment, group 5 received distilled water as a negative control, and group 6 received glibenclamide as a positive control. Medications are given for six days. Glucose levels were measured, and observation of pancreatic Langerhans cell damages.Results:  A decrease in blood glucose levels observed in all treatment groups. The most significant reduction (49.76%; 1000 mg/kg BW) occurred in the P3 group. Morphological features of pancreatic Langerhans cell damage were slightly high in the P1 group.Conclusion: P. malayana Jack can consider having an antidiabetic effect in a type 1 diabetic rat by reducing blood glucose levels.

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