scholarly journals Evaluation of Different Brands of Cefixime Available in Pakistan by Newly Developed Spectrophotometric Method

2021 ◽  
pp. 179-185
Author(s):  
Madan Lal Maheshwari ◽  
Ubed-ur-Rahman Mughal ◽  
Geeta Kumari ◽  
Bilawal Shaikh ◽  
Maryam Qazi ◽  
...  
Keyword(s):  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Pharmaceutical Preparations ◽  
Molar Absorptivity ◽  
Percentage Recovery ◽  
Derivatizing Agent ◽  
Derivatization Reaction

A fast, simple and valued method is developed to observe the quality and quantity of different pharmaceutical brands of cefixime. A spectrophotometric method has been developed for analysis of cefixime (CFX) by reacting with 4-dimethylamino benzaldehyde (DAB) as derivatizing agent. The molar absorptivity of CFX-DAB, newly synthesized derivative was calculated as 3.2 x 105 L.mole-1cm-1 and λ maximum was 393 nm. The calibration curve was developed in range of 5-25 µg.mL-1as this concentration followed beers law. The derivatization reaction is stable and didn’t show any difference in absorbance with radiation interaction for up to one day. The percentage recovery of CFX was checked and calculated in different pharmaceuticals was within 95 to 99.5% with RSD value calculated in between RSD 0.69-0.96% (n=3), respectively. This newly developed and validated procedure was proved to be accurate and precise for the analysis of CFX. This method was successfully applied to check amount of CFX from 7 different brands of pharmaceutical preparations commercially available in Pakistan.

scholarly journals Difference spectrophotometric method for the determination of Fluphenazine hydrochloride in tablets using peroxomonosulfate

2021 ◽  
Vol 9 (2) ◽  
pp. 64-71
Author(s):  
Mykola Blazheyevskiy ◽  
◽  
Valeriy Moroz ◽  
Olena Mozgova ◽  
◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Present Method ◽  
Molar Absorptivity ◽  
Limit Of Quantification ◽  
Derivatizing Agent ◽  
Sensitive Method

The oxidative derivatization method using potassium hydrogenperoxomonosulfate for the indirect spectrophotometric determination of Fluphenazine hydrochloride is presented. Potassium hydrogenperoxomonosulfate is introduced as a derivatizing agent for Fluphenazine hydrochloride, yielding the sulfoxide. This reaction product was successfully used for the spectrophotometric determination of the Fluphenazine hydrochloride. The UV spectroscopic detection of the sulfoxide proved to be a more robust and sensitive method. The elaborated method allowed the determination of Fluphenazine hydrochloride in the concentration range of 0.2-30 µg mL-1. The molar absorptivity at 349 nm is 5.6×103 (dm3cm-1mol-1). The limit of quantification, LOQ (10S) is 0.24 µg/mL. A new spectrophotometric technique was developed and the possibility of quantitative determination of Fluphenazine hydrochloride in tablets 5.0 mg was demonstrated. The present method is precise, accurate and excipients did not interfere. RSD for Fluphenazine Hydrochloride 5.0 mg tablets was 1.37 %.

scholarly journals Flotation-Spectrophotometric Method for the Determination of Aluminium ion Using Xylenol Orange

2011 ◽  
Vol 8 (4) ◽  
pp. 1528-1535 ◽  
Author(s):  
F. Nekouei ◽  
Sh. Nekouei
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Xylenol Orange ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Ion Associate ◽  
Sensitive Method

A simple, fast, reproducible and sensitive method for the flotation- spectrophotometric determination of Al3+is reported. The apparent molar absorptivity (ε) of the ion associate was determined to be 8.35×104L mol-1cm-1. The calibration curve was linear in the concentration range of 1.0-50 ng mL-1of Al3+with a correlation coefficient of 0.9997. The limit of detection (LOD) was 0.621 ng mL. The relative standard deviation (RSD) at 10 and 30 ng mL-1of aluminium were 1.580 and 2.410% (n=7) respectively. The method was applied for measuring the amount of aluminium in water samples.

scholarly journals New Spectrophotometric Method for Estimation of Ceftazidime in pure and pharmaceutical dosage

2019 ◽  
Vol 30 (3) ◽  
pp. 47
Author(s):  
Nibras Sabah Mohammed ◽  
Mohammed Jasim M. Hassan ◽  
Amer Saleh Mahdi
Keyword(s):  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Coupling Reaction ◽  
Molar Absorptivity ◽  
Local Market ◽  
Optimal Conditions ◽  
Limit Of Quantification ◽  
Commercial Preparation ◽  
Accuracy And Precision ◽  
Colored Compound

A new simple, rapid, inexpensive and nontoxic spectrophotometric method for thedetermination of Ceftazidime in the pure and local market formulation was proposed. Themethod is based on the coupling reaction between the diazotized drug and 4-tert-butylphenol(4-TBP) to produce a colored compound with a (λ max 500 nm). The optimal conditions for thefactors affecting the formation of this compound were studied. Beer's law was obeyed and alinear calibration curve was obtained over the range of (1–10 μg/mL) with a correlationcoefficient (r=0.9890), molar absorptivity (ε=6.62710 3 L/mol.cm), the limit of detection0.314 μg/mL, and the limit of quantification LOQ 1.047 μg/mL. the accuracy and precision ofthe method were checked and the method was applied successfully for the determination ofCeftazidime in commercial preparation and the values of recovery percent and RSD% rangedbetween (93.11 - 102.20%) and (0.48 - 9.54%) respectively.

scholarly journals Spectrophotometric method for determination of ranitidine hydrochloride in bulk and in pharmaceutical preparation using ninhydrin

2020 ◽  
Vol 11 (4) ◽  
pp. 291-297
Author(s):  
Hutaf Mustafa Baker ◽  
Hussam Ahmad Alsaoud ◽  
Hamzeh Mohamad Abdel-Halim
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Molar Absorptivity ◽  
Limit Of Quantification ◽  
Ranitidine Hydrochloride ◽  
Relative Standard ◽  
Reproducible Method

A simple, sensitive and reproducible method for the determination of ranitidine hydrochloride in pharmaceutical preparations was investigated. This spectrophotometric method was based on the formation of a deep red color product with ninhydrin in basic media and the absorbance measured at λmax = 480 nm. The reaction occurs at 45 °C with pH = 10 having a contact time of 38 minutes. Under the optimum conditions, Beer’s Law is obeyed in the concentration range of 8.98×103 - 9.90×104 µg/L. The coefficient of correlation was found to be 0.999 for the obtained method with molar absorptivity of 3.05×103 L/mol.cm. The calculated Sandell’s sensitivity is 0.108 μg/cm2. The limit of detection and limit of quantification are 0.0997 and 0.3023 µg/mL, respectively. The low values of the percentage relative standard deviation and percentage relative error indicate the high precision and the good accuracy of the proposed method. The stoichiometry of the reaction is determined and found to be 1:4 (Ranitidine hydrochloride:Ninhydrin). The initial rate method confirmed that this reaction is first order one.

scholarly journals A New Spectrophotometric Method for Analysis of Allopurinol in Aqueous Solutions and Pharmaceutical Preparations

2009 ◽  
Vol 6 (2) ◽  
pp. 309-314
Author(s):  
Baghdad Science Journal
Keyword(s):  
Absorption Spectrum ◽  
Aqueous Solutions ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Pharmaceutical Preparations ◽  
High Sensitivity ◽  
Good Stability ◽  
Optimum Conditions ◽  
Wide Range

A new method for determination of allopurinol in microgram level depending on its ability to reduce the yellow absorption spectrum of (I-3) at maximum wavelength ( ?max 350nm) . The optimum conditions such as "concentration of reactant materials , time of sitting and order of addition were studied to get a high sensitivity ( ? = 27229 l.mole-1.cm-1) sandal sensitivity : 0.0053 µg cm-2 ,with wide range of calibration curve ( 1 – 9 µg.ml-1 ) good stability (more then24 hr.) and repeatability ( RSD % : 2.1 -2.6 % ) , the Recovery % : ( 98.17 – 100.5 % ) , the Erel % ( 0.50 -1.83 % ) and the interference's of Xanthine , Cystein , Creatinine , Urea and the Glucose in 20 , 40 , 60 fold of analyate were also studied .

scholarly journals DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF DPP-4 INHIBITOR, SITAGLIPTIN, IN ITS PHARMACEUTICAL PREPARATIONS

2018 ◽  
Vol 35 (3) ◽  
pp. 45
Author(s):  
C. Bala Sekaran ◽  
A. Prameela Rani
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Amino Group ◽  
Reaction Conditions ◽  
Colored Product ◽  
Primary Amino ◽  
Development And Validation

A simple, sensitive and reproducible spectrophotometric method was developed for the determination of sitagliptin phosphate in bulk and in pharmaceutical formulations. The proposed method is based on condensation of the primary amino group of sitagliptin phosphate with acetyl acetone and formaldehyde producing a yellow colored product, which is measured spectrophotometrically at 430nm. The color was stable for about 1 hour. Beer’s law is obeyed over a concentration range of 5-25 μg/ml. The apparent molar absorptivity and Sandell sensitivity values are 1.067 x 104 Lmol-1cm-1 and 0.0471 μgcm-2 respectively. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the method was tested by analyzing sitagliptin phosphate in its pharmaceutical preparations. Good recoveries were obtained. The developed method was successfully employed for the determination of sitagliptin phosphate in various pharmaceutical preparations.

scholarly journals Leishman’s Dye as a Novel Reagent in Spectrophotometric Determination of Chloramphenicol

2020 ◽  
Vol 10 (02) ◽  
pp. 250-254
Author(s):  
Jamal Sudad Raeek Othman Nabeel Sabeeh
Keyword(s):  
Spectrophotometric Method ◽  
Acidic Medium ◽  
Pharmaceutical Preparations ◽  
High Sensitivity ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Sensitivity Index ◽  
Oxidizing Agent ◽  
Drug Compounds

By reviewing the literature, there is no indication concerning the use of Leishman’s dye in evaluating drug compounds by dye-color bleaching; hence, it is the first attempt to use Leishman’s dye as a novel reagent in the estimation of chloramphenicol (CAP) by an indirect spectrophotometric method in bulk and in its pharmaceutical preparations. The method includes the use of a great amount of N-bromosuccinamide (NBS) in the acidic medium as an oxidizing agent of the drug under investigation (CAP), and then using the residual of NBS for Leishman’s dye color bleaching. The absorbance has been measured at 622 nm (the maximum absorption of Leishman’s dye). A linear relationship was obtained for the Beer’s law with the concentration ranges from 10 to 250 μg/10 mL with acceptable values of molar absorptivity 0.58 × 104 L.mol-1.cm-1 and 0.055 μg.cm-2 of Sandell’s sensitivity index, which mean a high sensitivity. An approved estimation of CAP in its various pharmaceutical formulations was found.

scholarly journals Simple UV Spectrophotometric Determination of Rosuvastatin Calcium in Pure Form and in Pharmaceutical Formulations

2009 ◽  
Vol 6 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Alka Gupta ◽  
P. Mishra ◽  
K. Shah
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pure Form ◽  
Ultraviolet Region ◽  
Rosuvastatin Calcium ◽  
Sensitive Spectrophotometric Method

A new, simple and sensitive spectrophotometric method in ultraviolet region has been developed for the determination of rosuvastatin calcium in bulk and in pharmaceutical formulations. Rosuvastatin exhibits absorption maxima at 244 nm with apparent molar absorptivity of 7.2345 ×104L/mol.cm in methanol. Beer’s law was found to be obeyed in the concentration range of 2-18 µg/mL. The method is accurate, precise and economical. This method is extended to pharmaceutical preparations. In this method, there is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated statistically and by recovery studies

scholarly journals Spectrophotometric determination of Valsartan in pure form and in its pharmaceutical preparations

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Qabas Rashid ◽  
Ruwaida Farman Salih
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pure Form ◽  
Chromogenic Reagent

An easy, rapid and economical spectrophotometric method for  determination of  Valsartan (Val), by reaction with 4-chloro-7-nitrobenzofurazan (NBD-Cl) as reagent in an alkaline interemediate. This method is based on the forming of product between (Val) and the chromogenic reagent (NBD-Cl), to produce a brown color at (pH 11.9) and λmax. 470 nm.  Beer’s Law is obeyed at the concentrations range of (0.4-14.8 µg/ml), with molar absorptivity of (1.05×104 L/mol.cm) and correlation coefficient 0.9827, The limit of detection was 0.557 µg/ml. The suggested method was prosperity implement to the determination of (Val) in  pure form and in its pharmaceutical formulations (tablets).

scholarly journals A New Oxidative Derivatization Method For The Indirect Spectrofluorimetric Determination Of Prochlorperazine Maleate In Pharmaceutical Preparations

2019 ◽  
Vol 14 (3) ◽  
pp. 140-145
Author(s):  
M.Ye. Blazheyevskiy ◽  
Yu.V. Skrypynets ◽  
A.V. Yegorova ◽  
V.P. Antonovich
Keyword(s):  
Sulfuric Acid ◽  
Acid Solution ◽  
Quantitative Determination ◽  
Calibration Curve ◽  
Pharmaceutical Preparations ◽  
Limit Of Quantification ◽  
Highly Sensitive ◽  
Spectrofluorimetric Determination ◽  
Derivatizing Agent

A new oxidative derivatization method for the indirect spectrofluorimetric determination of Prochlorperazine maleate has been presented. Potassium hydrogenperoxomonosulphate is proposed as a derivatizing agent for Prochlorperazine, yielding the strongly fluorescent sulfoxide. This reaction product was successfully employed for the spectrofluorimetric determination of the Prochlorperazine maleate. A highly sensitive, simple and rapid method has been developed for determining prochlorperazine maleate in tablets by fluorescence of its oxidation product with Oxone solution in 0.01 M sulfuric acid solution (λex = 340 nm; λem = 380 nm). The calibration curve is linear in its concentration range of 0.8–10.0 µg/ml. Limit of quantification (LOQ = 10S) is 0.8 µg/ml. The possibility of quantitative determination of Prochlorperazine maleate in Vertinex® tablets 5 mg has been shown, RSD <2.3% (δ <RSD).

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