scholarly journals Tolerance of Some Soil Fungi to the Content of Deep Cycle Battery and Their Bioremediation Potential

2020 ◽  
pp. 34-46
Author(s):  
S. I. Douglas ◽  
C. U. Wellington ◽  
T. G. Sokari
Keyword(s):  
Screening Test ◽  
Soil Fungi ◽  
Metal Removal ◽  
Heavy Metal Removal ◽  
Soil Samples ◽  
State University ◽  
Candida Sp ◽  
Fungal Isolates ◽  
Microbiological Techniques ◽  
Aspergillus Sp

Aims: The purpose of this study was to isolate and screen soil fungi that are able to tolerate the contents of spent deep cycle battery (inverter), and to test for their bioremediation potential. Place and Duration of Study: Sample: Department of Microbiology, Rivers State University, between June 2019 and February 2020. Methodology: Soil samples were collected from a mechanic village while spent inverter batteries were obtained from a waste vendor. The battery was forced open to extract its contents of the battery. Using standard microbiological techniques, fungi were enumerated and characterized. Stock solution of the battery content was prepared by dissolving the inverter battery content in sterile deionized water. This stock solution was used to carry out the screening test on the fungal isolates to ascertain the fungi that can tolerate the contents of the spent battery. Results: Total heterotrophic fungal counts for the polluted and unpolluted soil were 6.0 x 103 cfu/g and 7.5 x 104cfu/g respectively. The fungal isolates identified from the polluted soil samples were members of the genera Rhizopus, Mucor, Aspergillus, Penicillium, and Candida, while, the isolates identified from the unpolluted soil sample includes: Candida sp, Aspergillus niger, Penicillium sp, Aspergillus fumigatus, Aspergillus flavus, Mucor sp, Yeast, Fusarium sp and Aspergillus sp. After the screening, total heterotrophic fungal counts for the soil ranged from 1.0 x 102cfu/g to 9.5 x 102cfu/g. Two fungi of the genera: Rhizopus and Mucor had the highest counts during 72 hours of incubation for the screening test. The results obtained from this study indicated that species of Aspergillus, Penicillium, and Candida were the most inhibited by the contents of the spent battery while Rhizopus and Mucor spp were more tolerant to the contents of the inverter. Rhizopus and Mucor spp were therefore, adopted in the bioremediation of soil contaminated with contents from the battery. It was observed that Rhizopus and Mucor spp in a consortium had the highest percentage of heavy metal removal (or uptake) in the following order: Cadmium (66.66%) > Lead (38.15%) > Zinc (26.83%) > Nickel (20.83). Conclusion: These organisms can be used in the bioremediation of soil polluted with metals from spent deep cycle batteries.

scholarly journals Fungal Population and Physicochemical Characteristics of Abattoir-Impacted Soil in Iwofe, Rivers State

2021 ◽  
pp. 36-44
Author(s):  
Williams, Janet Olufunmilayo ◽  
Owhorji, Gloria
Keyword(s):  
Heavy Metals ◽  
Aspergillus Niger ◽  
Physicochemical Characteristics ◽  
Control Soil ◽  
Fungal Population ◽  
Candida Sp ◽  
Fungal Isolates ◽  
Fungal Load ◽  
Microbiological Techniques ◽  
Rivers State

Aim: To determine the fungal population and physicochemistry of abattoir impacted soil in Iwofe, Rivers State. Study Design: This study focused on Abattoir impacted soil. Statistical analysis of data and interpretation was carried out. Place and Duration of Study: Abattoir impacted soil was collected from three points in an abattoir located in Iwofe, Rivers State while the unpolluted soil which served as control was collected from the Rivers State University, Port Harcourt in January, 2021. Methodology: Standard microbiological techniques were used: the fungal population was determined by inoculating aliquots of an appropriate dilution resulting from a ten-fold serial dilution on prepared Sabouraud dextrose agar plates in duplicates. Plates were later incubated for 3-5 days after which colonies were enumerated and used in obtaining the fungal population in the soil samples while distinct colonies were subcultured for macroscopic and microscopic identification of fungi. The physicochemical parameters and heavy metals were analyzed using standard methods. Results: Fungal load in the control and abattoir impacted soil were 1.09×105 and 3.9×104 CFU/g, respectively. The fungal load of the control soil was significantly higher (P˂0.05) than the abattoir impacted soil. The fungal isolates identified in the abattoir impacted soil were Microsporium sp, Aspergillus niger and Candida sp while Aspergillus niger, Aspergillus flavus, Fusarium sp, Penicillium sp, Mucor sp and Rhizopus sp were identified from the control soil. The pH, temperature, nitrate and phosphate of the abattoir soil were 6.7, 28.33℃, 27.83(mgKg-1) and 1055(mgKg-1), respectively. The concentrations of Cadmium, Iron and Lead in the abattoir Impacted soil and control soil were 0.81, 563.35 and 7.12 mgKg-1, 0.51, 582.0 and 3.18 mgKg-1, respectively. The physico chemistry and heavy metals in the abattoir soil were within acceptable limits. Discussion and Conclusion: The findings from this study showed that heavy metals in abattoir impacted soil had an impact in the fungal population which led to the isolation of only three fungal isolates belonging to Microsporium sp, Candida sp and Aspergillus niger. More so, despite the presence of heavy metals in the abattoir impacted soil, the metals were all within permissible limits. Thus, the abattoir impacted soil was not heavily polluted.

scholarly journals Insecticidal Action of Several Isolates of Entomopathogenic Fungi against The Granary Weevil Sitophilus Granarius

Agriculture ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 222 ◽  
Author(s):  
Spiridon Mantzoukas ◽  
Ioannis Lagogiannis ◽  
Maria Mpekiri ◽  
Ioannis Pettas ◽  
Panagiotis A. Eliopoulos
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Soil Samples ◽  
Conidial Suspension ◽  
Sitophilus Granarius ◽  
Granary Weevil ◽  
Fungal Isolates ◽  
Insecticidal Action ◽  
Lethal Time ◽  
Aspergillus Sp

The insecticidal virulence of various entomopathogenic fungal isolates retrieved from soil samples was tested on adults of the granary weevil Sitophilus granarius (L.) (Coleoptera: Curculionidae). Bioassays were carried out in the laboratory where experimental adults were sprayed with 1 mL of conidial suspension (108 conidia/mL) from each isolate. Mortality was recorded at 7, 14, and 21 days after exposure. Mean mortality, mean lethal time, survival, and hazard effect were estimated for each isolate. Two isolates of Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae), one isolate of Aspergillus insuetus (Bainier) Thom & Church (Eurotiales: Trichocomaceae) and Metarhizium anisopliae (Metschinkoff) Sorokin (Hypocreales: Clavicipitaceae) resulted in the highest mortality (97–100%). The isolates with both the highest hazard effect and the lowest survival rate were Aspergillus sp. and M. anisopliae. Our results indicate that entomopathogenic fungi have the potential to become a very useful tool in reducing chemical applications in storage facilities.

Microbial Biosurfactants-an Ecofriendly Boon to Industries for Green Revolution

2020 ◽  
Vol 14 (3) ◽  
pp. 169-183
Author(s):  
Pushpinder Sharma ◽  
Nivedita Sharma
Keyword(s):  
Metal Removal ◽  
Green Revolution ◽  
Heavy Metal Removal ◽  
Industrial Applications ◽  
Potential Candidate ◽  
Biological Origin ◽  
Environmental Cleanup ◽  
Candida Sp ◽  
Surface Active Agents ◽  
Potential Applications

Biosurfactants have a biological origin, and are widely known as surface active agents. Different classes of biosurfactant have significant importance in both the biotechnological and microbiological arena. Pseudomonas aeruginosa, Bacillus subtilis and Candida sp. are important classes of microorganisms that are highly investigated for the production of rhamnolipids (RLs) biosurfactants. Rhamnolipids have unique surface activity and have gained interest in various industrial applications. Due to their high biodegradability, renewability and functionally maintenance at extreme conditions, microbial biosurfactants are more advantageous than chemical-based biosurfactants. Biosurfactants produced by microorganisms are a potential candidate for biodegradation, environmental cleanup of pollutants and also play a role in the heavy metal removal of metallurgical industries also many patents have been filed. Therefore, greater attention has been paid to biosurfactants and identifying their potential applications for further studies.

scholarly journals The identification of soil fungi isolated from rhizosphere in different varieties of jali (Coix lacryma-jobi) in Loa Kulu, Kutai Kartanegara, Indonesia

2018 ◽  
Vol 2 (02) ◽  
pp. 44-47
Author(s):  
SOPIALENA SOPIALENA ◽  
N. AKHSAN ◽  
A. SURYADI ◽  
JULI NURDIANA
Keyword(s):  
Soil Fungi ◽  
Microbial Population ◽  
Organic Materials ◽  
Soil Samples ◽  
Rhizosphere Effect ◽  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp

Sopialena, Akhsan N, Suryadi A, Nurdiana J. 2018. The identification of soil fungi isolated from Rhizosphere in different varieties of jali plants (Coix lacryma-jobi) in Loa Kulu, Kutai Kartanegara, Indonesia. Asian J Agric 2: 44-47. The rhizosphere effect indicated the development of active microbial population around the roots driving from the loss of organic materials. This research was intended to identify rhizosphere microbes by identifying the potential microbes four different varieties of jali (Coix lacryma-jobi L.) in Loh Sumber, Loa Kulu, Kutai Kartanegara, Indonesia, i.e., jelai beras, jelai ketan, ketan lekat, and jelai kaltara. The soil samples were collected and isolated in the area of jali plantation in Loa Kulu, Kutai Kartanegara at 45 days after planting. As many as 16 isolated samples were used, collected from four different variety of jali and 4 times of replication. The result showed that a variety of microbes at Rhizosphere could be classified as Trichoderma sp. Aspergillus sp., Pythium sp., Fusarium sp., Cunninghamelal sp., and Penicillium sp..

scholarly journals Synthesis, characterization and heavy metal removal efficiency of nickel ferrite nanoparticles (NFN’s)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Waheed Ali Khoso ◽  
Noor Haleem ◽  
Muhammad Anwar Baig ◽  
Yousuf Jamal
Keyword(s):  
Heavy Metals ◽  
Nickel Ferrite ◽  
Contact Time ◽  
Metal Removal ◽  
Heavy Metal Removal ◽  
Ferrite Nanoparticles ◽  
Batch Adsorption ◽  
Aqueous Environment ◽  
Human Beings ◽  
Nickel Ferrite Nanoparticles

AbstractThe heavy metals, such as Cr(VI), Pb(II) and Cd(II), in aqueous solutions are toxic even at trace levels and have caused adverse health impacts on human beings. Hence the removal of these heavy metals from the aqueous environment is important to protect biodiversity, hydrosphere ecosystems, and human beings. In this study, magnetic Nickel-Ferrite Nanoparticles (NFNs) were synthesized by co-precipitation method and characterized using X-Ray Diffraction (XRD), Energy Dispersive Spectroscopy (EDS) and Field Emission Scanning Electronic Microscopy (FE-SEM) techniques in order to confirm the crystalline structure, composition and morphology of the NFN’s, these were then used as adsorbent for the removal of Cr(VI), Pb(II) and Cd(II) from wastewater. The adsorption parameters under study were pH, dose and contact time. The values for optimum removal through batch-adsorption were investigated at different parameters (pH 3–7, dose: 10, 20, 30, 40 and 50 mg and contact time: 30, 60, 90, and 120 min). Removal efficiencies of Cr(VI), Pb(II) and Cd(II) were obtained 89%, 79% and 87% respectively under optimal conditions. It was found that the kinetics followed the pseudo second order model for the removal of heavy metals using Nickel ferrite nanoparticles.

Research trends of heavy metal removal from aqueous environments

2021 ◽  
Vol 287 ◽  
pp. 112322
Author(s):  
Morteza Nazaripour ◽  
Mir Amir Mohammad Reshadi ◽  
Seyed Ahmad Mirbagheri ◽  
Mehdi Nazaripour ◽  
Alireza Bazargan
Keyword(s):  
Heavy Metal ◽  
Metal Removal ◽  
Heavy Metal Removal ◽  
Research Trends ◽  
Aqueous Environments
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