Bubble vent localization for marine hydrocarbon seep surveys

Commercial success of marine seep hunting exploration campaigns involves acquisition of high-quality bathymetry and backscatter along with targeted coring of seep sediments. The sharp lateral chemical gradient encompassing seafloor seeps requires accurate identification of seep sites from high-resolution acoustic data. Active seeps featuring plumes of gas bubbles and oil droplets rising into the water column can be imaged in modern multibeam echosounders providing an effective approach to remotely characterizing seafloor seeps. Interpreting the seafloor position of gas plume emissions in multibeam data using existing mapping methodology is hindered by slow processing due to large files sizes, a manual “by eye” qualitative assessment of each sonar ping searching for plume anomalies, skill and fatigue of the geoscientist, and environmental or acquisition artifacts that can mask the precise location of gas emission on the seafloor. These limitations of midwater backscatter mapping create a qualitative dataset with varying inherent positional errors that can lead to missed or incorrect observations about seep-related seafloor features and processes. By vertically integrating midwater multibeam amplitude samples, a two-dimensional midwater backscatter raster can be generated and draped over seafloor morphology, providing a synoptic overview of the spatial distribution of gas plume emission sites for improved interpretation. A multibeam midwater dataset from NOAA Cruise EX1402L2 in the northwestern Gulf of Mexico is reprocessed using a vertical amplitude stacking technique. Midwater backscatter surfaces are compared to digitized plume positions collected during the survey for a comparison into assessing uncertainty in mapping approaches and an assessment of uncertainty. Results show that the accuracy of digitized geopicks over selected plume clusters vary considerably when compared to the midwater backscatter amplitude maps. This mapping technique offers multiple advantages over traditional geopicking from cost-effectiveness, offshore efficiency, repeatability, and higher accuracy, ultimately improving the detectability and sampling of active seafloor seeps through precisely located cores.

Formulation of trade-off relationships between resolution, line edge roughness, and sensitivity in sub-10 nm half-pitch region for chemically amplified extreme ultraviolet resists

The manufacturing of semiconductor devices using extreme ultraviolet (EUV) lithography started in 2019. A high numerical aperture (NA) tool under development is capable of resolving 8 nm line-and-space optical images and will extend the application of EUV lithography. However, resist materials have not been yet applicable to the production with 8 nm resolution. In this study, the relationships among the half-pitch of line-and-space patterns (resolution), chemical gradient [an indicator of line edge roughness (LER)], and sensitivity were investigated in the sub-10 nm half-pitch region for chemically amplified EUV resists. The chemical gradient was simulated on the basis of their sensitization and reaction mechanisms. The relationship was formulated as a function of total sensitizer concentration (the sum of photoacid generator and photodecomposable quencher concentrations) and the thermalization distance of secondary electrons. The effect of thermalized electrons was well incorporated into the trade-off relationships between resolution, LER, and sensitivity.

Development of a Microfluidic Device to Form a Long Chemical Gradient in a Tissue from Both Ends with an Analysis of Its Appearance and Content

Tissue assays have improved our understanding of cancers in terms of the three-dimensional structures and cellular diversity of the tissue, although they are not yet well-developed. Perfusion culture and active chemical gradient formation in centimeter order are difficult in tissue assays, but they are important for simulating the metabolic functions of tissues. Using microfluidic technology, we developed an H-shaped channel device that could form a long concentration gradient of molecules in a tissue that we could then analyze based on its appearance and content. For demonstration, a cylindrical pork tissue specimen was punched and equipped in the H-shaped channel device, and both ends of the tissue were exposed to flowing distilled and blue-dyed water for 100 h. After perfusion, the tissue was removed from the H-shaped channel device and sectioned. The gradient of the blue intensity along the longitudinal direction of the tissue was measured based on its appearance and content. We confirmed that the measured gradients from the appearance and content were comparable.

Three-dimensional Cancer Cell Migration Directed by Dual Mechanochemical Guidance

Directed cell migration guided by external cues plays a central role in many physiological and pathophysiological processes. The microenvironment of cells often simultaneously contains various cues and the motility response of cells to multiplexed guidance is poorly understood. Here we combine experiments and mathematical models to study the three-dimensional migration of breast cancer cells in the presence of both contact guidance and a chemoattractant gradient. We find that the chemotaxis of cells is complicated by the presence of contact guidance as the microstructure of extracellular matrix (ECM) vary spatially. In the presence of dual guidance, the impact of ECM alignment is determined externally by the coherence of ECM fibers, and internally by cell mechanosensing Rho/Rock pathways. When contact guidance is parallel to the chemical gradient, coherent ECM fibers significantly increase the efficiency of chemotaxis. When contact guidance is perpendicular to the chemical gradient, cells exploit the ECM disorder to locate paths for chemotaxis. Our results underscores the importance of fully characterizing the cancer cell microenvironment in order to better understand invasion and metastasis.

Beyond Janus Geometry: Characterization of Flow Fields Around Nonspherical Photocatalytic Microswimmers

Catalytic microswimmers that move by a phoretic mechanism in response to a self-induced chemical gradient are often obtained by the design of spherical janus microparticles, which suffer from multi-step fabrication and low yields. Approaches such as irregular particle shapes, local excitation or intrinsic asymmetry are on the rise to facilitate manufacturing, but the effects on the generation of motion remain poorly understood. In this work, single crystalline BiVO4 microswimmers are presented that rely on a strict inherent asymmetry of charge-carrier distribution under illumination. The origin of the asymmetrical flow pattern is elucidated becauseof the high spatial resolution of measured flow fields around pinned BiVO4 colloids. As a result the flow from oxidative to reductive particle sides was confirmed. Distribution of oxidation and reduction reactions suggests a dominant self-electrophoretic motion mechanism with a source quadrupole as the origin of the induced flows. It is shown that the symmetry of the flow fields is broken by self-shadowing of the particles and synthetic surface defects that impact the photocatalytic activity of the microswimmers. The results demonstrate the complexity of symmetry breaking in nonspherical microswimmers and are leading the way towards understanding ofpropulsion mechanisms of phoretic colloids of various shapes.

Electrotaxis-on-Chip to Quantify Neutrophil Migration Towards Electrochemical Gradients

Electric fields are generated in vivo in a variety of physiologic and pathologic settings, including wound healing and immune response to injuries to epithelial barriers (e.g. lung pneumocytes). Immune cells are known to migrate towards both chemical (chemotaxis), physical (mechanotaxis) and electric stimuli (electrotaxis). Electrotaxis is the guided migration of cells along electric fields, and has previously been reported in T-cells and cancer cells. However, there remains a need for engineering tools with high spatial and temporal resolution to quantify EF guided migration. Here we report the development of an electrotaxis-on-chip (ETOC) platform that enables the quantification of dHL-60 cell, a model neutrophil-like cell line, migration toward both electrical and chemoattractant gradients. Neutrophils are the most abundant white blood cells and set the stage for the magnitude of the immune response. Therefore, developing engineering tools to direct neutrophil migration patterns has applications in both infectious disease and inflammatory disorders. The ETOC developed in this study has embedded electrodes and four migration zones connected to a central cell-loading chamber with migration channels [10 µm X 10 µm]. This device enables both parallel and competing chemoattractant and electric fields. We use our novel ETOC platform to investigate dHL-60 cell migration in three biologically relevant conditions: 1) in a DC electric field; 2) parallel chemical gradient and electric fields; and 3) perpendicular chemical gradient and electric field. In this study we used differentiated leukemia cancer cells (dHL60 cells), an accepted model for human peripheral blood neutrophils. We first quantified effects of electric field intensities (0.4V/cm-1V/cm) on dHL-60 cell electrotaxis. Our results show optimal migration at 0.6 V/cm. In the second scenario, we tested whether it was possible to increase dHL-60 cell migration to a bacterial signal [N-formylated peptides (fMLP)] by adding a parallel electric field. Our results show that there was significant increase (6-fold increase) in dHL60 migration toward fMLP and cathode of DC electric field (0.6V/cm, n=4, p-value<0.005) vs. fMLP alone. Finally, we evaluated whether we could decrease or re-direct dHL-60 cell migration away from an inflammatory signal [leukotriene B4 (LTB4)]. The perpendicular electric field significantly decreased migration (2.9-fold decrease) of dHL60s toward LTB4vs. LTB4 alone. Our microfluidic device enabled us to quantify single-cell electrotaxis velocity (7.9 µm/min ± 3.6). The magnitude and direction of the electric field can be more precisely and quickly changed than most other guidance cues such as chemical cues in clinical investigation. A better understanding of EF guided cell migration will enable the development of new EF-based treatments to precisely direct immune cell migration for wound care, infection, and other inflammatory disorders.

Dynamic manipulation of droplets using mechanically tunable microtextured chemical gradients

Materials and strategies applicable to the dynamic transport of microdroplets are relevant to surface fluidics, self-cleaning materials, thermal management systems, and analytical devices. Techniques based on electrowetting, topographic micropatterns, and thermal/chemical gradients have advanced considerably, but dynamic microdroplet transport remains a challenge. This manuscript reports the fabrication of mechano-tunable, microtextured chemical gradients on elastomer films and their use in controlled microdroplet transport. Specifically, discreet mechanical deformations of these films enabled dynamic tuning of the microtextures and thus transport along surface-chemical gradients. The interplay between the driving force of the chemical gradient and the microtopography was characterized, facilitating accurate prediction of the conditions (droplet radius and roughness) which supported transport. In this work, the use of microtextured surface chemical gradients in mechano-adaptive materials with microdroplet manipulation functionality was highlighted.

Convective core entrainment in 1D main sequence stellar models

3D hydrodynamics models of deep stellar convection exhibit turbulent entrainment at the convective-radiative boundary which follows the entrainment law, varying with boundary penetrability. We implement the entrainment law in the 1D Geneva stellar evolution code. We then calculate models between 1.5 and 60 M⊙ at solar metallicity (Z = 0.014) and compare them to previous generations of models and observations on the main sequence. The boundary penetrability, quantified by the bulk Richardson number, RiB, varies with mass and to a smaller extent with time. The variation of RiB with mass is due to the mass dependence of typical convective velocities in the core and hence the luminosity of the star. The chemical gradient above the convective core dominates the variation of RiB with time. An entrainment law method can therefore explain the apparent mass dependence of convective boundary mixing through RiB. New models including entrainment can better reproduce the mass dependence of the main sequence width using entrainment law parameters A ∼ 2 × 10−4 and n = 1. We compare these empirically constrained values to the results of 3D hydrodynamics simulations and discuss implications.