The Cortical Motor System in the Domestic Pig: Origin and Termination of the Corticospinal Tract and Cortico-Brainstem Projections

The anatomy of the cortical motor system and its relationship to motor repertoire in artiodactyls is for the most part unknown. We studied the origin and termination of the corticospinal tract (CST) and cortico-brainstem projections in domestic pigs. Pyramidal neurons were retrogradely labeled by injecting aminostilbamidine in the spinal segment C1. After identifying the dual origin of the porcine CST in the primary motor cortex (M1) and premotor cortex (PM), the axons descending from those regions to the spinal cord and brainstem were anterogradely labeled by unilateral injections of dextran alexa-594 in M1 and dextran alexa-488 in PM. Numerous corticospinal projections from M1 and PM were detected up to T6 spinal segment and showed a similar pattern of decussation and distribution in the white matter funiculi and the gray matter laminae. They terminated mostly on dendrites of the lateral intermediate laminae and the internal basilar nucleus, and some innervated the ventromedial laminae, but were essentially absent in lateral laminae IX. Corticofugal axons terminated predominantly ipsilaterally in the midbrain and bilaterally in the medulla oblongata. Most corticorubral projections arose from M1, whereas the mesencephalic reticular formation, superior colliculus, lateral reticular nucleus, gigantocellular reticular nucleus, and raphe received abundant axonal contacts from both M1 and PM. Our data suggest that the porcine cortical motor system has some common features with that of primates and humans and may control posture and movement through parallel motor descending pathways. However, less cortical regions project to the spinal cord in pigs, and the CST neither seems to reach the lumbar enlargement nor to have a significant direct innervation of cervical, foreleg motoneurons.

Biomechanical aspects of the initial stability of instrumental fixation in the treatment of subaxial cervical dislocations: an experimental study

Objective. To study the influence of thoracic inlet angle (TIA) and the fracture of the articular process on the initial strength of the fixation of the spinal segment during its anterior and circular instrumental surgical stabilization in an experiment on a model of the lower cervical spinal segment.Material and Methods. The material of the study was assembled models of C6–C7 spinal segments made using addictive technologies by 3D printing. After preliminary instrumentation, spinal segments were installed on the stand testing machine using specially manufactured equipment. A metered axial load simulating the native one was applied along the axis of the parameters SVA COG–C7 and C2–C7 SVA, which values were close to the value of 20 mm, at a rate of 1 mm/min until the shear strain was reached. The system’s resistance to displacement was measured, and the resulting load was evaluated. Four study groups were formed depending on the modeling of the T1 slope parameter, the integrity of the facets, and the type of instrumentation. Three tests were conducted in each group. The graphical curves were analyzed, and the values of the parameters of the neutral and elastic zones, the yield point, time to yield point, and the value of the applied load for the implementation of shear displacement were recorded. The data were subjected to comparative analysis.Results. In Group 1, anterior shear displacement of the C6 vertebra could not be induced in all series. In groups 2, 3, and 4 a shear displacement of ≥4 mm was noted in all series. In Group 3 where a fracture of the articular process was additionally modeled, the average value of the yield point was 423.5 ± 46.8 N. Elastic zone, the time to the onset of the yield point, the time at the end point or at a shear of C6 ≥4 mm did not differ significantly. In Group 4, a translational displacement of ≥4 mm was observed, though the average yield point was 1536.0 ± 40.0 N.Conclusion. The direction of the load applied to the fixed spinal segment, as well as the presence of damage to the articular processes, play a crucial role in maintaining resistance to shear deformation of the spinal segment during its instrumental stabilization. At high values of TIA (T1 slope) and the presence of fractures of the articular processes, the isolated anterior stabilization is less effective, circular fixation of 360° under these conditions gives a high initial stability to the spinal segment.

A Simple and Effective Technique for Removing Broken Pedicle Screws

Background: Different methods and tools had been developed to remove the broken pedicle screws. Removing a broken pedicle screws was not always easy. We reported a simple and effective technique to remove the broken pedicle screws in this study.Methods: The Institutional Review Board of the Fengdu people's Hospital of Chongqing approved the study. A total of 34 patients (aged 47.76±8.12 years) accepted first operation because of thoracolumbar fractures in our department and other departments were analyzed retrospectively. They accepted secondary surgery to remove the spinal instrumentations when they obtained bone fusion or bone healing from March 2017 to March 2020. We applied a high speed drill (with 2mm diameter), a three rhomboid pointed cone, an acutenaculum, and a larger forceps to remove the broken pedicle screws.Results: The spinal segment of broken pedicle screws was consist of L2 (3 cases), L3 (8 cases), L4 (17 cases), L5 (6 cases). The location of the broken pedicle screws was within the scope of pedicle. There were no complications about broken pedicle screws, such as injury of nerve roots, severe low back pain, and spinal cord injury. The average time of removing the broken pedicle screws was 13.79±3.52 minutes. The mean blood loss of surgery was 72.06±28.05 milliliters. No other complications happened during surgery.Conclusion: A simple and effective technique for removing broken pedicle screws is reported. This technique reserves the original nail road of pedicle, does not destroy the pedicle cortex, and averts producing the debris of spinal instrumentations.

A finite element model of the human lower thorax to pelvis spinal segment: Validation and modal analysis

BACKGROUND: Several finite element (FE) models have been developed to study the effects of vibration on human lumbar spine. However, the authors know of no published results so far that have proposed computed tomography-based FE models of whole lumbar spine including the pelvis to conduct dynamic analysis. OBJECTIVE: To create and validate a three-dimensional ligamentous FE model of the human lower thorax to pelvis spinal segment (T12–Pelvis) and provide a detailed simulation environment to investigate the dynamic characteristics of the lumbar spine under whole body vibration (WBV). METHODS: The T12–Pelvis model was generated based on volume reconstruction from computed tomography scans and validated against the published experimental data. FE modal analysis was implemented to predict dynamic characteristics associated with the first-order vertical resonant frequency and vibration mode of the model with upper body mass of 40 kg under WBV. RESULTS: It was found that the current FE model was validated and corresponded closely with the published data. The obtained results from the modal analysis indicated that the first-order vertical resonant frequency of the T12–Pelvis model was 6.702 Hz, and the lumbar spine mainly performed vertical motion with a small anteroposterior motion. It was also found that shifting the upper body mass centroid onwards or rearwards from the normal upright sitting posture reduced the vertical resonant frequency. CONCLUSIONS: These findings may be helpful to better understand vibration response of the human spine, and provide important information to minimize injury and discomfort for these WBV-exposed occupational groups.

Surgery treatment of primary spinal tumors

To analyze the results of the surgical treatment of primary spinal tumors, based on the appropriate ASIA scales, SINS, Bilsky, considering the location of tumors in various segments of the vertebral column, a retrospective analysis of 43 patients with primary benign and malignant spinal tumours was performed. The results of treatment depending on the histological type of tumors and the possibility of radical removal of tumors within healthy tissues have been studied. It has been established that with timely radical surgical treatment it is possible to prevent neurological complications, improve existing neurological disorders, restore normal anatomical relationships in the affected spinal segment, and create a reliable, rigid stabilization of the spine, which in turn allows prolonging patients’ lives.

Lower spinal postural variability during laptop-work in subjects with cervicogenic headache compared to healthy controls

Spinal postural variability (SPV) is a prerequisite to prevent musculoskeletal complaints during functional tasks. Our objective was to evaluate SPV in cervicogenic headache (CeH) since CeH is characterized by such complaints. A non-randomized repeated-measure design was applied to compare SPV between 18 participants with reporting CeH aged 29–51 years, and 18 matched controls aged 26–52 years during a 30-min-laptop-task. Habitual spinal postures (degrees) of the cervical, thoracic and lumbar spine were analysed using 3D-Vicon motion analysis. SPV, to express variation in mean habitual spinal posture, was deducted from the postural analysis. Mean SPV of each spinal segment was lower in the CeH-group compared to the control-group. Within the CeH-group, SPV of all except one spinal segment (lower-lumbar) was higher compared to the group’s mean SPV. Within the control-group, SPV was more comparable to the group’s mean SPV. SPV differed between groups. Averaging data resulted in decreased SPV in the CeH-group compared to the control-group during the laptop-task. However, the higher within-group-SPV in the CeH-group compared to the group’s mean SPV accentuated more postural heterogeneity. It should be further determined if addressing individual SPV is a relevant intervention.

Alterations of Dopamine-Related Transcripts in A11 Diencephalospinal Pathways after Spinal Cord Injury

The diencephalic A11 nuclei are the primary source of spinal dopamine (DA). Neurons in this region project to all levels of the spinal cord. Traumatic spinal cord injury (SCI) often interrupts descending and ascending neuronal pathways and further elicits injury-induced neuronal plasticity. However, it is unknown how A11 neurons and projections respond to SCI-induced axotomy. Based on preliminary observation, we hypothesized that A11 DA-ergic neurons rostral to the lesion site might change their capacity to synthesize DA after SCI. Adult rats received a complete spinal cord transection at the 10th thoracic (T10) level. After 3 or 8 weeks, rostral (T5) and caudal (L1) spinal cord tissue was collected to measure mRNA levels of DA-related genes. Meanwhile, A11 neurons in the brain were explicitly isolated by laser capture microdissection, and single-cell qPCR was employed to evaluate mRNA levels in the soma. Histological analysis was conducted to assess the number of A11 DA-ergic neurons. The results showed that, compared to naïve rats, mRNA levels of tyrosine hydroxylase (TH), dopamine decarboxylase (DDC), and D2 receptors in the T5 spinal segment had a transient decrease and subsequent recovery. However, dopamine-β-hydroxylase (DBH), D1 receptors, and DA-associated transcription factors did not change following SCI. Furthermore, axon degeneration below the lesion substantially reduced mRNA levels of TH and D2 in the L1 spinal segment. However, DDC transcript underwent only a temporary decrease. Similar mRNA levels of DA-related enzymes were detected in the A11 neuronal soma between naïve and SCI rats. In addition, immunostaining revealed that the number of A11 DA neurons did not change after SCI, indicating a sustention of capacity to synthesize DA in the neuroplasm. Thus, impaired A11 diencephalospinal pathways following SCI may transiently reduce DA production in the spinal cord rostral to the lesion but not in the brain.