PENINGKATAN PRODUKTIVITAS SAPI POTONG MENGGUNAKAN PROBIOTIK UNTUK RUMINANSIA DI DESA WONGSOREJO DAN GOMBENGSARI KECAMATAN WONGSOREJO, KABUPATEN BANYUWANGI, JAWA TIMUR

The Banyuwangi Regency Government, through the Livestock Service Office, is very active in improving the community's economic level through the livestock sector. It is proven through a variety of dynamic population numbers in 2014 namely, 108,139 beef cattle. For this reason, it is necessary to get broad attention in terms of increasing the productivity of livestock. The success of increasing livestock productivity is determined by the factors of seed, feed, and management, while the largest component, which is 70%, comes from the feed factor. So far, it is rarely known that forage feed or additional feed in the form of tofu dregs or concentrate, is not completely digested in the small intestine but only about 60-70%, while the rest is excreted through feces and urine. For this reason, it is necessary to provide feed supplementation in the form of probiotics for ruminants containing several non-pathogenic microorganisms (bacteria) that can help increase feed digestibility. Probiotics can be defined as food supplements containing live microbes that have a beneficial effect on animals as their host through improving the balance of microflora in the digestive tract. Community service activities that have been carried out include the provision of probiotics for ruminants which are given directly to farmers by visiting the location of the beef cattle pen. The community service program is carried out for 5 months including counseling, training, evaluation, and cadre development. It can be concluded that there is an increase in cognitive, affective, psychomotor and substance aspects in the partner community.Keywords: beef cattle; Wongsorejo; Gombengsari; Banyuwangi AbstrakPemerintah Kabupaten Banyuwangi, melalui Dinas Peternakan sangat gencar dalam meningkatkan taraf perekonomian masyarakat melalui sektor peternakan. Terbukti melalui beragam jumlah populasi dinamis pada tahun 2014 yakni, sapi potong 108.139 ekor. Untuk itu perlu mendapat perhatian yang luas dalam hal peningkatan produktivitas hewan ternak. Keberhasilan peningkatan produktivitas peternakan ditentukan oleh faktor bibit, pakan dan pengelolaan, sedangkan komponen terbesar yaitu sebesar 70 % berasal dari faktor pakan. Selama ini jarang diketahui bahwa pakan hijauan maupun pakan tambahan berupa ampas tahu atau konsentrat, tidak seluruhnya dicerna dalam usus halus melainkan hanya sekitar 60-70 %,sedangkan yang sisanya dibuang lewat feses dan urine. Untuk itu perlu dilakukan pemberian suplementasi pakan berupa pro biotik untuk Ruminan yang mengandung beberapa mikroorganisme (bakteri) non patogen yang mampu membantu peningkatan kecernaan pakan. Probiotik dapat didefinisikan sebagai suplemen makanan yang mengandung mikrobial hidup menimbulkan efek menguntungkan hewan sebagai induk semangnya melalui peningkatan keseimbangan mikroflora di dalam saluran pencernaan. Kegiatan pengabdian kepada masyarakat yang telah dilakukan meliputi pemberian probiotik untuk ruminan yang diberikan secara langsung kepada peternak dengan mendatangi lokasi kandang sapi pedaging. Program pengabdian masyarakat dilakukan selama 5 bulan meliputi penyuluhan, pelatihan, evaluasi, dan pembinaan kader. Dapat disimpulkan terdapat peningkatan aspek kognitif, afektif, psikomotor dan substansi pada masyarakat mitra.Kata kunci: sapi potong; Wongsorejo; Gombengsari; Banyuwangi

Pan-genome analysis identifies intersecting roles for Pseudomonas specialized metabolites in potato pathogen inhibition

Agricultural soil harbors a diverse microbiome that can form beneficial relationships with plants, including the inhibition of plant pathogens. Pseudomonas spp. are one of the most abundant bacterial genera in the soil and rhizosphere and play important roles in promoting plant health. However, the genetic determinants of this beneficial activity are only partially understood. Here, we genetically and phenotypically characterize the Pseudomonas fluorescens population in a commercial potato field, where we identify strong correlations between specialized metabolite biosynthesis and antagonism of the potato pathogens Streptomyces scabies and Phytophthora infestans. Genetic and chemical analyses identified hydrogen cyanide and cyclic lipopeptides as key specialized metabolites associated with S. scabies inhibition, which was supported by in planta biocontrol experiments. We show that a single potato field contains a hugely diverse and dynamic population of Pseudomonas bacteria, whose capacity to produce specialized metabolites is shaped both by plant colonization and defined environmental inputs.

Using mobile phone data to estimate dynamic population changes and improve the understanding of a pandemic: A case study in Andorra

Compartmental models are often used to understand and predict the progression of an infectious disease such as COVID-19. The most basic of these models consider the total population of a region to be closed. Many incorporate human mobility into their transmission dynamics, usually based on static and aggregated data. However, mobility can change dramatically during a global pandemic as seen with COVID-19, making static data unsuitable. Recently, large mobility datasets derived from mobile devices have been used, along with COVID-19 infections data, to better understand the relationship between mobility and COVID-19. However, studies to date have relied on data that represent only a fraction of their target populations, and the data from mobile devices have been used for measuring mobility within the study region, without considering changes to the population as people enter and leave the region. This work presents a unique case study in Andorra, with comprehensive datasets that include telecoms data covering 100% of mobile subscribers in the country, and results from a serology testing program that more than 90% of the population voluntarily participated in. We use the telecoms data to both measure mobility within the country and to provide a real-time census of people entering, leaving and remaining in the country. We develop multiple SEIR (compartmental) models parameterized on these metrics and show how dynamic population metrics can improve the models. We find that total daily trips did not have predictive value in the SEIR models while country entrances did. As a secondary contribution of this work, we show how Andorra's serology testing program was likely impacted by people leaving the country. Overall, this case study suggests how using mobile phone data to measure dynamic population changes could improve studies that rely on more commonly used mobility metrics and the overall understanding of a pandemic.

Development of Micro-Spatial Electricity Load Forecasting Methodology Using Multivariate Analysis for Dynamic Area in Tangerang, Indonesia

Dynamic population and land use significantly affect future energy demand. This paper proposes a suitable method to forecast load growth in a dynamic area in Tangerang, Indonesia. This research developed micro-spatial load forecasting, which can show load centres in microgrids, estimate the capacity and locate the distribution station precisely. Homogenous grouping implemented the method into clusters consisted of microgrids. It involves multivariate variables containing 12 electric and non-electric variables. Multivariate analysis is conducted by carrying out Principal Component Analysis (PCA) and Factor Analysis. The forecasting results can predict load growth, time, and location, which can later be implemented as the basis of a master electricity distribution plan because it provides an accurate long-term forecast.

Subinhibitory Levels of Oxytetracycline in Earthworm Meal Significantly Boost Resistance-Mutation Rates in Bacillus spp. within the Gut of Eisenia fetida

Aims: To estimate changes in the number of oxytetracycline -resistant strains, a dynamic population of Bacillus in the gut of Eisenia fetida cultured in processed-cow-dung (PrCD) supplemented with subinhibitory amounts of oxytetracycline, was studied. Study Design: Sterile water containing oxytetracycline was sprayed over PrCD at concentration of 0.012 µg per mg dehydrated PrCD per spraying. The number of oxytetracycline-resistant Bacillus spp. microbiota of E. fetida’s gut was compared to the number of oxytetracycline-resistant Bacillus spp. in its feed (PrCD). For this purpose, Luria Agar (LA) and Bacillus Agar (BA) plates amended with or without oxytetracycline were used. Place and Duration of Study: The study was carried out in the Omics Laboratory of Department of Biotechnology, University of North Bengal situated in Darjeeling district of West Bengal, India during 2017-18. Methodology: E. fetida gut content and PrCD samples were collected on different days of the experiment for bacteriological analysis. Dilution plating on LA yielded the total number of cultivable heterotrophic bacteria. LA plates amended with oxytetracycline (15 µg/ml) were used to acquire the fraction of heterotrophic bacteria resistant to oxytetracycline. BA plates were also utilized to obtain the fraction of resistant Bacillus spp. population. The frequency of mutation was determined using a conventional formula. Results: In the gut of E. fetida reared in PrCD supplemented with sub-inhibitory concentration of oxytetracycline, a rise in the oxytetracycline-resistant Bacillus population was observed. On day 1, the frequency of oxytetracycline-resistant Bacillus spp. (5×10-8) matched the spontaneous mutation frequency, however higher frequencies on days 2 and 7 (1.6×10-4 and 3.5 x 10-6 respectively) suggested significant dissemination of oxytetracycline resistance in the gut environment. Conclusion: Subinhibitory oxytetracycline concentrations in earthworm diet had a significant effect on mutation rates, showing that evolutionary forces on the gut microbiota may be determining their responses to antibiotic stress.

Expression Atlas of Avian Neural Crest Proteins: Neurulation to Migration

Neural crest (NC) cells are a dynamic population of embryonic stem cells that create various adult tissues in vertebrate species including craniofacial bone and cartilage and the peripheral and enteric nervous systems. NC development is a conserved and complex process that is controlled by a tightly regulated gene regulatory network (GRN) of morphogens, transcription factors, and cell adhesion proteins. While multiple studies have characterized the expression of several GRN factors in single species, a comprehensive protein analysis that directly compares expression across development is lacking. To address this, we used three closely related avian models, Gallus gallus (chicken), Coturnix japonica (Japanese quail), and Pavo cristatus (Indian peafowl), to compare the localization and timing of four GRN transcription factors, PAX7, SOX9, SNAI2, and SOX10 from the onset of neurulation to migration. While the spatial expression of these factors is largely conserved, we find that quail NC cells express SOX9, SNAI2, and SOX10 proteins at the equivalent of earlier developmental stages than chick and peafowl. In addition, quail NC cells migrate farther and more rapidly than the larger organisms. These data suggest that despite a conservation of NC GRN players, differences in the timing of NC development between species remain a significant frontier to be explored with functional studies.

Marked Reductions in Pediatric Outpatient Antibiotic Prescribing in Portugal During the Pandemic

The COVID-19 pandemic brought unexpected effects on healthcare systems access and usage. Some of the measures implemented by governments to try and contain it, such as confinement periods, social distancing, widespread use of masks and other hygiene practices led to unprecedented epidemiological changes in several common infectious diseases.We describe changes in antibiotic prescribing in pediatric outpatients before and during the pandemic in a dynamic population analysis context during a study period of three years in mainland Portugal.There was a marked reduction in antimicrobial prescribing in all regions, particularly in younger children and during the lockdown periods.This reduction was much sharper for those antimicrobials most commonly used to treat respiratory-tract infections, as Amoxicillin (with and without β-lactamase inhibitor) and Macrolides, and considerable less accentuated in other commonly prescribed antibiotics as Co-Trimoxazole and Fosfomycin, usually associated with the treatment of urinary tract infections.Conclusion: Our findings indicate that the COVID-19 pandemic has had profound effects on antibiotic use in ambulatory care in children in Portugal.Further monitoring is necessary to ascertain whether this reduction in antibiotic prescription will persist and whether these changes may impact antimicrobial resistance.