Development of Predictive Geoarchaeological Models to Locate and Assess the Preservation Potential of Submerged Prehistoric Sites Using Remote Sensing, Palaeoenvironmental Analysis, and GIS

Using the Mesolithic site of Tudse Hage in the Great Belt of Denmark, this paper proposes a generic stepwise process to create geoarchaeological models that output seamless morphology maps in a GIS. This was achieved using remote sensing databases and the collection of marine geophysical data, above and below the seabed. On the basis of these data, key areas, with sediment sequences representative of the postglacial transgression surfaces, were identified. Core samples were taken for palaeoenvironmental analysis and dating that enabled a reconstruction of the relative sea-level changes. Using this information, palaeogeographic coastline maps of the Kongemose, late Kongemose, Ertebølle, and Neolithic periods in the Tudse Hage area were prepared, and potential hotspots for archaeological sites were proposed. Since their inundation, submerged prehistoric archaeological sites have been, and are, dynamic, with anthropogenic and natural processes affecting their stability and preservation. With the advocation of in situ preservation as a means of managing underwater cultural heritage, predicting where sites have survived these processes, and where they can be found, in advance of subsea development or other anthropogenic exploitation, is essential. Future natural threats to sites preserved in situ were determined through the modelling of seabed currents and sediment erosion.

Conservation of Waterlogged Wood—Past, Present and Future Perspectives

This paper reviews the degradation, preservation and conservation of waterlogged archaeological wood. Degradation due to bacteria in anoxic and soft-rot fungi and bacteria in oxic waterlogged conditions is discussed with consideration of the effect on the chemical composition of wood, as well as the deposition of sulphur and iron within the structure. The effects on physical properties are also considered. The paper then discusses the role of consolidants in preserving waterlogged archaeological wood after it is excavated as well as issues to be considered when reburial is used as a means of preservation. The use of alum and polyethylene glycol (PEG) as consolidants is presented along with various case studies with particular emphasis on marine artefacts. The properties of consolidated wood are examined, especially with respect to the degradation of the wood post-conservation. Different consolidants are reviewed along with their use and properties. The merits and risks of reburial and in situ preservation are considered as an alternative to conservation.

Underwater Power Tools for In Situ Preservation, Cleaning and Consolidation of Submerged Archaeological Remains

In situ protection and conservation of the Underwater Cultural Heritage are now considered a primary choice by the scientific community to be preferred, when possible, over the practice of recovery. The conservation of the artefacts within their environmental context is essential in fact for a correct interpretation of archaeological presences and to preserve their true value intact for future generations. However, this is not an easy task because modern technological equipment is necessary to make the work carried out by underwater restorers and archaeologists faster and more efficient. To this end, the paper presents three innovative underwater power tools for the cleaning, conservation, and consolidation activities to be performed in submerged archaeological sites. The first one is an underwater cleaning brush tool for a soft cleaning of the underwater archaeological structures and artefacts; the second one is a multifunctional underwater hammer drill suitable to be used as a corer sampler, chisel, or drill; the last one is an injection tool specifically designed to dispense mortar underwater for consolidation techniques of submerged structures.

A Full-Ocean-Depth Sampler for Hadal Microbes Based on Multistage Membrane Filters With In-Situ Preservation

In order to obtain high-quality microbial samples from the hadal zone, which has a depth of over 6,000 m, a full-ocean-depth sampler with the function of in-situ filtration and preservation was developed. A flow pump and several membrane filters were used for in-situ filtration under the sea. With a multistage filtering structure, the microbes can be initially screened according to their sizes. To avoid the degradation of microbial ribonucleic acid (RNA), a special structure was designed to inject the RNAlater solution into the samples immediately after the filtration. The sampler was tested in our laboratory and deployed during Mariana TS-15 in 2019. It was installed on a hadal lander of Shanghai Ocean University and deployed at MBR02 (11.371°N, 142.587°E, 10,931 m) in the Mariana Trench. A total of 20 L of in-situ seawater was filtered, and membranes with pore sizes of 3 and 0.2 μm were preserved. The study is expected to provide important support for the establishment of a hadal microbial gene pool.

Fossil microbial shark tooth decay documents in situ metabolism of enameloid proteins as nutrition source in deep water environments

Alteration of organic remains during the transition from the bio- to lithosphere is affected strongly by biotic processes of microbes influencing the potential of dead matter to become fossilized or vanish ultimately. If fossilized, bones, cartilage, and tooth dentine often display traces of bioerosion caused by destructive microbes. The causal agents, however, usually remain ambiguous. Here we present a new type of tissue alteration in fossil deep-sea shark teeth with in situ preservation of the responsible organisms embedded in a delicate filmy substance identified as extrapolymeric matter. The invading microorganisms are arranged in nest- or chain-like patterns between fluorapatite bundles of the superficial enameloid. Chemical analysis of the bacteriomorph structures indicates replacement by a phyllosilicate, which enabled in situ preservation. Our results imply that bacteria invaded the hypermineralized tissue for harvesting intra-crystalline bound organic matter, which provided nutrient supply in a nutrient depleted deep-marine environment they inhabited. We document here for the first time in situ bacteria preservation in tooth enameloid, one of the hardest mineralized tissues developed by animals. This unambiguously verifies that microbes also colonize highly mineralized dental capping tissues with only minor organic content when nutrients are scarce as in deep-marine environments.

Evidence of proteins, chromosomes and chemical markers of DNA in exceptionally preserved dinosaur cartilage

A histological ground-section from a duck-billed dinosaur nestling (Hypacrosaurus stebingeri) revealed microstructures morphologically consistent with nuclei and chromosomes in cells within calcified cartilage. We hypothesized that this exceptional cellular preservation extended to the molecular level and had molecular features in common with extant avian cartilage. Histochemical and immunological evidence supports in situ preservation of extracellular matrix components found in extant cartilage, including glycosaminoglycans and collagen type II. Furthermore, isolated Hypacrosaurus chondrocytes react positively with two DNA intercalating stains. Specific DNA staining is only observed inside the isolated cells, suggesting endogenous nuclear material survived fossilization. Our data support the hypothesis that calcified cartilage is preserved at the molecular level in this Mesozoic material, and suggest that remnants of once-living chondrocytes, including their DNA, may preserve for millions of years.