Long-Term Amelioration Practices Reshape the Soil Microbiome in a Coastal Saline Soil and Alter the Richness and Vertical Distribution Differently Among Bacterial, Archaeal, and Fungal Communities

Globally soil salinity is one of the most devastating environmental stresses affecting agricultural systems and causes huge economic losses each year. High soil salinity causes osmotic stress, nutritional imbalance and ion toxicity to plants and severely affects crop productivity in farming systems. Freezing saline water irrigation and plastic mulching techniques were successfully developed in our previous study to desalinize costal saline soil. Understanding how microbial communities respond during saline soil amelioration is crucial, given the key roles soil microbes play in ecosystem succession. In the present study, the community composition, diversity, assembly and potential ecological functions of archaea, bacteria and fungi in coastal saline soil under amelioration practices of freezing saline water irrigation, plastic mulching and the combination of freezing saline water irrigation and plastic mulching were assessed through high-throughput sequencing. These amelioration practices decreased archaeal and increased bacterial richness while leaving fungal richness little changed in the surface soil. Functional prediction revealed that the amelioration practices, especially winter irrigation with saline water and film mulched in spring, promoted a community harboring heterotrophic features. β-null deviation analysis illustrated that amelioration practices weakened the deterministic processes in structuring coastal saline soil microbial communities. These results advanced our understanding of the responses of the soil microbiome to amelioration practices and provided useful information for developing microbe-based remediation approaches in coastal saline soils.

Excavated farmland with plastic mulching as a strategy in saving water and controlling soil salinization in dryland agricultural areas

Water shortage and soil salinization in gully farmland comprising sediment deposited farmland (SF) and excavated farmland (EF) have become a widespread concern in the loess hilly region. A two-year field experiment was conducted to assess the soil water content (SWC) and salt content (SSC) and their effect on the spring maize yield and water use efficiency in SF and EF. Eight treatments comprising flat cropping without mulching (1), ridge planting without mulching (2), ridge planting with plastic mulching (3), and ridge planting with straw mulching (4) were tested in the SF and EF plots, respectively. The results showed that the yield was higher in SF than EF, whereas the water use efficiency was significantly higher in EF because the bottom water flux was 117.4% higher in SF than EF (P < 0.01). A significant positive correlation was found between the average SWC and yield (P < 0.01), thereby indicating that the yield was severely limited by the SWC. Thus, the higher water use efficiency in EF has important implications for alleviating water scarcity during agricultural production in this region. The risk of soil salinization was decreased greatly by treatment 3 where the SSC was decreased in EF and SF were 0.09 g kg–1 and 0.08 g kg–1, respectively. In addition, treatment 3 had the most significant impacts on the yield and water use efficiency. Our study provided appropriate land type and effective tillage measure for the sustainable development in dryland agricultural areas.

Study of the Effect of Mulching Materials on Weed Control in Saffron Cultivation in Eastern Morocco

Saffron (Crocus sativus L.) is cultivated in many countries for its culinary and medicinal values. The production of saffron is limited by several factors, including weed infestation, which causes damage to the crop in terms of quantity and quality. However, little information is available on the different weed management strategies for saffron cultivation, as most of the strategies implemented are developed for large-scale and conventional agriculture. As a result, they are not applicable or affordable for organic or smallholder farmers, as is the case for saffron cultivation. The objective of this study is to compare the effectiveness of plastic mulching versus mulching in controlling weeds in saffron cultivation in the eastern region of Morocco. During the trial, which was conducted in 2018, the parameters measured correspond, on the one hand, to morphometric measurements and determination of saffron stigma yield and, on the other hand, to the determination of density, dry biomass, and weed control capacity. Compared to the control, mulching reduced the population and dry biomass of the most formidable weeds such as Cynodon dactylon, Aster squamatus, Cyperus rotundus, and Convolvulus arvensis. The average stigmata yield from plastic mulch treatment was 9% higher than of the control, and the number of leaves, leaf area, number, weight, and percentage of daughter corms with large diameter were higher for plants grown under mulch. Overall, the results of this study showed that the use of PE (polyethylene) mulch effectively reduced weed populations and improved saffron yield and vegetative growth.

Simulation of Soil Water and Heat Flow under Plastic Mulching and Different Ridge Patterns

The ridge–furrow mulching system with plastic film (RFMS) has been widely used in semi-arid areas in order to improve soil water and heat conditions, crop yields and water use efficiency. It is of practical significance to study the effect of mulching and ridge types on soil water and heat in order to optimize mulching measures and improve the effectiveness of the ridge and furrow system. To clarify the combined effect of soil water and heat beneath the system and the influence of ridge morphology on it, field experiments were conducted with three treatments, including conventional planting in bare land (CK), a ridge–furrow (wide ridge with 70 cm width and 10 cm height, narrow ridge with 40 cm width and 15 cm height) mulching system with complete plastic film (RFWN) and a ridge–furrow (equal ridge with 55 cm width and 15 cm height) mulching system with complete plastic film (RFE). An insufficient irrigation system was adopted and the two-dimensional numerical software HYDRUS-2D was used to simulate the soil water and heat flow under the experimental conditions. The model was calibrated and verified according to test data for the period of 2018 to 2019, which showed good agreement between the simulated and measured values. The simulation results revealed that the ground temperatures of RFWN and RFE were much higher than that of CK, and the average value of 0–25 cm during the growth period could increase by 2.29–4.61%. Compared with CK, RFWN and RFE reduced soil evaporation (84.71–93.73%) and field evapotranspiration (12.02–21.75%), while they increased root water uptake (25.87–40.98%) and T/ET (48.85–80.15%). Plastic film mulching and ridge morphologies affected the infiltration range and the direction of soil water movement, increased soil moisture when there was no rainfall or irrigation and reduced soil water and heat fluctuations, which was more conducive to crop growth, especially under the RFWN system. The simulation method proposed in this paper is an effective technique for calculating the soil water and heat dynamics under different ridge and furrow sections under the condition of film mulching, and it can be used for the optimal management of soil water and heat in this area.

Effect of Plastic Mulching on Soil Carbon and Nitrogen Cycling-Related Bacterial Community Structure and Function in a Dryland Spring Maize Field

Plastic mulching, given its positive effects on temperature and water retention, has been widely used to solve water shortages and nutrient scarcity in rainfed agricultural soils. This practice affects the physical and chemical processes of soil, including carbon and nitrogen cycling. However, research into microbe-mediated carbon and nitrogen cycling in soil with plastic mulching is still limited. In this study, the structures and functions of the soil bacterial community in non-mulched spring maize, plastic-mulched spring maize, and bareland fallow in a dryland field on the Loess Plateau in China were analyzed to explore the responses of microbe-mediated carbon and nitrogen cycling to plastic mulching. Results showed that the richness of soil bacteria was the highest in bareland fallow. Plastic mulching increased the diversity and richness of soil bacteria to a certain extent (p > 0.05), and significantly increased the content of microbial biomass nitrogen (MBN) (p < 0.05). Plastic mulching enhanced the total abundances of carbon and nitrogen cycling-related microbes, exhibiting a significant increase in the abundances of Cellvibrio, Bacillus, Methylobacterium and Nitrospira (p < 0.05). Predicted functional analysis revealed 299 metabolic pathways related to carbon and nitrogen cycling, including methane metabolism, carbon fixation in photosynthetic organisms, and nitrogen metabolism. The number of gene families assigned to carbon and nitrogen cycling-related metabolic pathways was higher in plastic mulched than that in non-mulched spring maize. This study demonstrated that plastic mulching enhances the capacity of carbon and nitrogen cycling, revealing its potential in mediating greenhouse gas emissions in the dryland spring maize fields on the Loess Plateau.