Secretome of Stressed Peripheral Blood Mononuclear Cells Alters Transcriptome Signature in Heart, Liver, and Spleen after an Experimental Acute Myocardial Infarction: An In Silico Analysis

Acute myocardial infarction (AMI) is a result of cardiac non-perfusion and leads to cardiomyocyte necrosis, inflammation, and compromised cardiac performance. Here, we showed that the secretome of γ-irradiated peripheral blood mononuclear cells (PBMCsec) improved heart function in a porcine AMI model and displayed beneficial long- and short-term effects. As an AMI is known to strongly affect gene regulation of the ischemia non-affected heart muscle and distal organs, we employed a transcriptomics approach to further study the immediate molecular events orchestrated using the PBMCsec in myocardium, liver, and spleen 24 h post ischemia. In the infarcted area, the PBMCsec mainly induced genes that were essential for cardiomyocyte function and simultaneously downregulated pro-inflammatory genes. Interestingly, genes associated with pro-inflammatory processes were activated in the transition zone, while being downregulated in the remote zone. In the liver, we observed a pronounced inhibition of immune responses using the PBMCsec, while genes involved in urea and tricarboxylic cycles were induced. The spleen displayed elevated lipid metabolism and reduced immunological processes. Together, our study suggested several types of pharmacodynamics by which the PBMCsec conferred immediate cardioprotection. Furthermore, our data supported the assumption that an AMI significantly affects distal organs, suggesting that a holistic treatment of an AMI, as achieved by PBMCsec, might be highly beneficial.

A Sustainable Approach for the Production of Green Energy With the Holistic Treatment of Wastewater Through Microbial Electrochemical Technologies: A Review

With the plausible depletion of fossil fuels in the near future and its associated environmental impacts, researchers have instigated the search for eco-friendly renewable bioenergy. Moreover, the increase in water pollution by industrial and anthropogenic activities is another alarming global concern. In this regard, the production of renewable and sustainable green bioenergy utilizing wastewater through microbial electrochemical technologies (METs) can alleviate these crucial problems by providing a sustainable solution to meet both the demands of energy and fresh water supply. Moreover, different bio-centered techniques such as nitrification and denitrification for nitrogen removal, and elimination of carcinogenic metals, pathogens, and organic components utilizing microbiota followed by toxicity sensing of different pollutants have been efficaciously exhibited through METs. However, inferior bioenergy production and recovery of low biomass yield in METs with high operational cost are noteworthy bottlenecks that hinder the scalability of this technology. Therefore, this review elaborates different physicochemical factors affecting the performance of METs, microbial interaction for the development of stable biofilm and so forth. Moreover, a broad overview on the production of bioenergy, along with the removal of pollutants from wastewater through different types of METs are also highlighted. Furthermore, the production of biofuels like ethanol, methanol, biodiesel, and gaseous fuel like bio-H2 coupled with power generation using photosynthetic microorganisms via CO2 sequestration through METs are also discussed. Additionally, recent developments with future scope for the field-scale implementation of METs along with their bottlenecks have been discussed, which has not been critically reviewed to date.

Inflammatory bowel disease negatively impacts household and family life

BackgroundInflammatory bowel disease (IBD) has a major impact on different aspects of life of patients with IBD, but functioning in the home environment is under-reported in literature. The aim of this study was to assess the impact of IBD on household and/or family life.MethodAdult patients with IBD from the outpatient clinic of the Erasmus MC (Rotterdam, the Netherlands) were invited to participate in this cross-sectional study between September and December 2020. A questionnaire was composed to measure the impact of IBD on functioning in the household and family life. In addition, the IBD Disability Index and Work Productivity and Activity Impairment Questionnaire were used. Data were analysed using descriptive statistics and multiple logistic regression modelling.ResultsOf 308 invited patients, 229 patients were included (response rate 74%). Sixty-eight per cent of patients were diagnosed with Crohn’s disease, 69% were female and median age was 38.0 years. Many patients reported difficulties with domestic activities (55.0%), leisure (53.9%), parenting (50.6%), sexual activities (48.4%) and interpersonal relationships (39.1%). Fatigue was associated with the experience of more severe difficulties in domestic activities (OR 16.160, 95% CI 6.053 to 43.141). Furthermore, 32.7% of patients felt that IBD influenced their desire to have children.ConclusionIBD has a markedly negative impact on household and/or family life in many patients. To optimise patient care, household and family related difficulties need to be considered in a holistic treatment approach.

Collaborative Healthcare in Incarcerated Settings

A total of 2,162,400 adults were incarcerated in the United States in 2016. Sub-optimal health status, existing healthcare disparities, and fragmented healthcare delivery among incarcerated populations are concerning and warrant redress. This article highlights the need for and discusses the benefits of collaboration between healthcare professionals in incarcerated settings. The roles of primary care health professionals, pharmacists, and medical family therapists (MedFTs) in correctional facilities are outlined. Through integrated healthcare models, enhanced communication, improved continuity of care, and holistic treatment plans, existing gaps in healthcare delivery in correctional facilities can be filled. By working together and assuming nontraditional roles, medical professionals can help improve health outcomes of incarcerated individuals. Collaborative healthcare models in incarcerated settings can elevate public health in a cost-effective, yet positive manner.

bonpa indigenous cure system of sowa rigpa or ‘science of healing’ practices

The Tibetan medicinal system is among the oldest known healing traditions practiced in the world. History is witness to the fact that there is a vast treasure trove of indigenous knowledge and practices. It offers a holistic treatment system that is aimed at regulating and maintaining an astute balance of the functional principles defining human existence through mind and body. Indigenous medicine is an integral part of the Tibetan culture and has grown and developed across several centuries and geographical spaces. The origins of Tibetan system that pertain to the Bon religious tenets are apparently very old as aspects of the Tibetan medicine have been traced to ancient cultures over 2,500 years old. Traditionally the Tibetan curative system has been known as Sowa-Rigpa Medicine meaning the ‘Science of Healing’. “It is a centuries old system of treatment that is based on a complex approach to diagnosis, incorporating techniques like taking the pulse and conducting an analysis of bodily fluids besides making an examination of the behavior and dietary intake. The medicines are composed of natural materials (e.g., herbs, minerals, plants, leaves etc) and physical therapies (e.g. Tibetan acupuncture, moxabustion, etc) to treat illness.

Comparative Analysis Study of Snozelen Therapy and Five Senses Theraphy as an Alternative Therapy Using Multisensory Stimulation Factors: Focusing on Intervention Research on Snozelen Therapy

Modern people inherently carry stress, psychological anxiety, and lack of communication. Therapy is a resource that can relieve stress and psychological anxiety for easing such tension. This is one of the reasons why modern people desperately need therapy. During the "healing" process through therapy, people experience an improvement in defense against diseases and natural healing, which leads to holistic treatment that integrates physical, psychological, social, and spiritual healing. Therapy has been widely used in five senses therapy that stimulates multiple sensations However, in addition to the five senses, Snoezelen therapy that includes dynamic sensations has recently emerged. Snozelen therapy can be applied as an interventional method for relaxation, stress prevention and management in the field of psychological health, as well as being more widely used as part of a variety of sensory stimulation therapies for people with physical problems.oThere are now numerous therapy pouring out for well-being and improved quality of life, but comparative research for perception, importance, and understanding of therapy has been insufficient, making it difficult to select the therapy that suits oneself. Therefore, we hope that the comparative analysis of Snoezelen therapy and five senses therapy in this study will serve as an opportunity to elevate the quality of life with the enhanced understanding and perception of therapy and more effective therapy treatment.

Late Onset Acquired Prothrombin Complex Deficiency (APCD) in a 27 Days Old Infant: a Rare Case Report

Acquired prothrombin complex deficiency (APCD) is a disease that occurs due to a deficiency in the coagulation factors that make up the prothrombin complex (II, VII, IX, and X), which are highly dependent on the presence of vitamin K in carrying out their functions. This study uses a qualitative method with the type of case report. The sampling technique used in this study the author uses the Random Sampling technique or by using the Slovin formula in Husein Umar. Where each population has the same opportunity to be selected as a sample in this study. Based on the analysis and discussion, it can be concluded that APCD is a disease with a high mortality rate, and those who survive are also threatened with disability for the rest of their lives. So that early detection and holistic treatment need to be done to prevent death and disability.

Review of Mechanisms, Pharmacological Management, Psychosocial Implications, and Holistic Treatment of Pain in Fabry Disease

Fabry disease is a progressive X-linked lysosomal storage disease caused by a mutation in the GLA gene, encoding the lysosomal hydrolase α-galactosidase A. The consequent reduced enzyme activity results in the toxic accumulation of glycosphingolipids, particularly globortriaosylceramide (Gb3 or GL3), in blood vessels, renal epithelia, myocardium, peripheral nervous system, cornea and skin. Neuropathic pain is the most common manifestation of Fabry disease and can be extremely debilitating. This often develops during childhood and presents with episodes of burning and sharp pain in the hands and feet, especially during exercise and it is worse with increased heat or fever. It is thought to be due to ischaemic injury and metabolic failure, leading to the disruption of neuronal membranes and small fibre neuropathy, caused by a reduced density of myelinated Aδ and unmyelinated C-fibres and alterations in the function of ion channels, mediated by Gb3 and lyso Gb3. It is important to confirm small fibre neuropathy before any Fabry disease treatment modality is considered. There is a clinical need for novel techniques for assessing small fibre function to improve detection of small fibre neuropathy and expand the role of available therapies. The current Fabry disease guidelines are in favour of pharmacological management as the first-line treatment for pain associated with Fabry disease. Refractory cases would benefit from a rehabilitation approach with interdisciplinary input, including medical, physiotherapy and psychological disciplines and including a Pain Management Programme.

Cross-Sectional Design: Link Between Parkinson’s Pain and Restless Leg Syndrome

Objective: Prior research suggests that restless leg syndrome (RLS) is prevalent in Parkinson’s disease (PD) with insufficient evidence to support the relationship between RLS, PD, and pain. This study explored the relationship between pain in PD patients and its association with the prevalence and severity of RLS. Method: 127 PD patients were assessed for PD and RLS using the U.K. Brain bank Criteria and the Restless Leg Syndrome diagnostic criteria, respectively. These patients were also assessed for pain perception and interference using the Brief Pain Inventory. Results: The results demonstrated Parkinson’s disease patients who reported pain scored 23 more Restless Leg Syndrome prevalence points ( p < 0.05), and 8.5 counts higher for Restless Leg Syndrome severity ( p < 0.05) compared to the group of Parkinson’s disease patients denying pain. Discussion: The presence of pain in PD patients indicated a higher RLS prevalence and an increased RLS severity. This finding suggests patients suffering from pain interference may experience more severe RLS symptoms. This demonstrates an inextricable link and association between pain in PD patients and RLS. Further robust investigations are required to elucidate any potential causative links, which can inform more holistic treatment principles.

SPINAL TUBERCULOSIS REVISTED : STUDY OF CLINICALPRESENTATION, DIAGNOSIS, AND MANAGEMENT OF SPINAL TUBERCULOSIS IN A TERTIARY CARE CENTRE IN SOUTH INDIA

Introduction: Tuberculosis is a disease that affects mostly young individuals in their productive formative years. The risk is 20–37 times greater in HIV co- infected individuals than among those without HIV. Spinal tuberculosis is uncommon in the western world as compared to Asian countries. But it is e-emerging in Western Countries due to the HIV pandemic. Aim Of The Study: This study analysis the clinical presentation, neuroradiology manifestations, and treatment aspects of spinal tuberculosis. Design: Observational study. Materials And Methods: Patients admitted in government Royapettah hospital during the year July 2018- February 2020 in the general medical ward with the diagnosis of paraplegia / paraparesis and subsequently diagnosed with spinal tuberculosis were recruited for the study. 35 of them were diagnosed to have spinal tuberculosis after Inclusion and exclusion criteria. Detailed history was taken, examination was done and appropriate imaging (MRI Spine, CT Chest) was taken. Results: 1 (2.9 %) patient had intramedullary lesion in the form of longitudinally extensive transverse myelitis. 34 (97.1 %) patients had extra medullary lesion. 26 (74.3 %) patients underwent medical management. All of them were started on CAT I ATT DOTS regimen along with steroids. 9 (25.7 %) of them underwent both surgical and medical management. Conclusion: The prime aim of treatment of spinal tuberculosis is prevention of deformity so that patients can have a better quality of life. Paramount to this is a high index of suspicion, early detection and initiation of treatment. A holistic treatment approach in all cases of spinal tuberculosis is needed to prevent this dreaded complication.