Birds of a Feather Eat Plastic Together: High Levels of Plastic Ingestion in Great Shearwater Adults and Juveniles Across Their Annual Migratory Cycle

Limited work to date has examined plastic ingestion in highly migratory seabirds like Great Shearwaters (Ardenna gravis) across their entire migratory range. We examined 217 Great Shearwaters obtained from 2008–2019 at multiple locations spanning their yearly migration cycle across the Northwest and South Atlantic to assess accumulation of ingested plastic as well as trends over time and between locations. A total of 2328 plastic fragments were documented in the ventriculus portion of the gastrointestinal tract, with an average of 9 plastic fragments per bird. The mass, count, and frequency of plastic occurrence (FO) varied by location, with higher plastic burdens but lower FO in South Atlantic adults and chicks from the breeding colonies. No fragments of the same size or morphology were found in the primary forage fish prey, the Sand Lance (Ammodytes spp., n = 202) that supports Great Shearwaters in Massachusetts Bay, United States, suggesting the birds directly ingest the bulk of their plastic loads rather than accumulating via trophic transfer. Fourier-transform infrared spectroscopy indicated that low- and high-density polyethylene were the most common polymers ingested, within all years and locations. Individuals from the South Atlantic contained a higher proportion of larger plastic items and fragments compared to analogous life stages in the NW Atlantic, possibly due to increased use of remote, pelagic areas subject to reduced inputs of smaller, more diverse, and potentially less buoyant plastics found adjacent to coastal margins. Different signatures of polymer type, size, and category between similar life stages at different locations suggests rapid turnover of ingested plastics commensurate with migratory stage and location, though more empirical evidence is needed to ground-truth this hypothesis. This work is the first to comprehensively measure the accumulation of ingested plastics by Great Shearwaters over the last decade and across multiple locations spanning their yearly trans-equatorial migration cycle and underscores their utility as sentinels of plastic pollution in Atlantic ecosystems.

Diving behavior of humpback whales (Megaptera novaeangliae) throughout their migratory cycle in the Western South Atlantic Ocean

Is poorly known how the diving behavior during the migratory cycle of humpback whales Megaptera novaeangliae is affected by environmental characteristics of their seasonal habitats and their social roles. In this study, archival Argos satellite tags were deployed off the coast of Brazil in 32 humpback whales from the South Western Atlantic Ocean. Tags reported data for ~44 days (max = 124 days) and 92,058 dives. Statistical analysis showed that average dive depth varied between habitats, being shallower in the breeding area (BA, 22.6 m), intermediate during the migration (MI, 30.26 m), and deeper in the feeding area (FA, 35.16 m). The dives classified as deep (>80 m), were more predominant during migration. At FA, dives during the night were shallower and shorter than those performed during the day, at BA dives made during the day were deeper than all other diel phases. We have not found clear differences in dive depth and duration between social classes. The moon phases had a clear effect within BA but not within MI and FA, being shallower at full moon. We also saw that the shapes (U, V, Square), showed differences in dive depth and among habitats. Our data provide the first novel insights into the dive behaviors of individuals from the same population throughout their migratory cycle, performing different diving behaviors, different patterns of social class, shapes, depth range, diel and moon phase. Moreover, these findings and the understanding diving patterns in its drivers have important implications for ecological modelling, conservation policies.

Influenza A Viruses in Ruddy Turnstones (Arenaria interpres); Connecting Wintering and Migratory Sites with an Ecological Hotspot at Delaware Bay

Each May for over three decades, avian influenza A viruses (IAVs) have been isolated from shorebirds and gulls (order Charadriiformes) at Delaware Bay (DE Bay), USA, which is a critical stopover site for shorebirds on their spring migration to arctic breeding grounds. At DE Bay, most isolates have been recovered from ruddy turnstones (Arenaria interpres), but it is unknown if this species is involved in either the maintenance or movement of these viruses outside of this site. We collected and tested fecal samples from 2823 ruddy turnstones in Florida and Georgia in the southeastern United States during four winter/spring sample periods—2010, 2011, 2012, and 2013—and during the winters of 2014/2015 and 2015/2016. Twenty-five low pathogenicity IAVs were recovered representing five subtypes (H3N4, H3N8, H5N9, H6N1, and H12N2). Many of these subtypes matched those recovered at DE Bay during the previous year or that year’s migratory cycle, suggesting that IAVs present on these southern wintering areas represent a source of virus introduction to DE Bay via migrating ruddy turnstones. Analyses of all IAV gene segments of H5N9 and H6N1 viruses recovered from ruddy turnstones at DE Bay during May 2012 and from the southeast during the spring of 2012 revealed a high level of genetic relatedness at the nucleotide level, suggesting that migrating ruddy turnstones move IAVs from wintering grounds to the DE Bay ecosystem.

Modeling Migratory Patterns of the Eastern Monarch Butterfly

The purpose of this research was to relate the influence of specific site suitability variables to eastern monarch butterfly migratory patterns and behavior. Elevation, temperature, precipitation, and land use data layers were overlaid to collectively consider how these variables affected the way that butterflies migrated and recolonized during the 2016/2017 migratory cycle. The variables were reclassified into layers ranking suitability as either unsuitable, suitable, or optimal with respective scores of one, three, and five. Three uninhabitable variables were identified that deemed a site unsuitable despite the influence and possible optimal suitability of the other variables. The results of this study indicated that site suitability was a large driving factor for migratory monarchs with a heavier emphasis placed on average temperature and land/cropland use. Possible displaced and sink populations were identified for further study, while the effects of agriculture, development, and climate change were considered regarding flyway connectivity and behavior.

Host age shapes virome abundance and diversity in birds

Host age influences the ecology of many microorganisms. This is evident in one-host – one virus systems, such as influenza A virus in Mallards, but also in community studies of parasites and microbiomes. We used a meta-transcriptomic approach to assess whether host age is associated with differences in the abundance and diversity of avian viromes. We used samples from cohabiting Ruddy Turnstones (Arenaria interpres) across three age groups, collected at two contrasting points in their annual migratory cycle. Overall, we identified 14 viruses that likely infect birds, 11 of which were novel, including members of the Reoviridae, Astroviridae, Picornaviridae, and Phenuiviridae. Strikingly, 12 of the viruses identified were from juvenile birds sampled in the first year of their life, compared to only two viruses in adult birds. Similarly, both viral abundance and alpha diversity were higher in juvenile than adult birds. Notably, time of sampling had no association with virome structure such that the migratory period may not play a major role in structuring avian viromes. As well as informing studies of virus ecology, that host age impacts viral assemblages is a critical consideration for the future surveillance of novel and emerging viruses.

International migration movements

The issue of international migration has been increasingly present, especially since the early 2010s, in the daily news. However, the media tends to focus on a small minority of these movements, those which arise from exoduses linked to international wars or to civil wars often fanned – voluntarily or involuntarily – by external powers, and those which take place in non-legal forms, the main cause of which is most often poor governance. Beyond that, it is important to take a complete and fair look at all international migration in daily life. However, this does not entail following sensationalist claims that depict a world entering a generalised migratory cycle. Finally, we should ask ourselves about the real changes undergone by international migration in the 21st century.

Spatio-temporal ecological niche modelling of multigenerational insect migrations

Modelling ecological niches of migratory animals requires incorporating a temporal dimension, in addition to space. Here, we introduce an approach to model multigenerational migratory insects using time-partitioned environmental variables (by months and years) and time- and behaviour-partitioned records (breeding records to model reproductive habitat). We apply this methodology to modelling the Palearctic-African migratory cycle of the Painted Lady butterfly ( Vanessa cardui ), based on data encompassing 36 years (646 breeding sites from 30 countries). Each breeding record is linked to a particular time (month and year), and the associated values of the bioclimatic variables are used for an ensemble modelling strategy, to finally obtain monthly projections. The results show obligated movements, mostly latitudinal, for the species' successive generations across the overall range, and only scattered locations show high probabilities of reproduction year-round. The southernmost reproductive areas estimated for the Palearctic-African migratory pool reach equatorial latitudes from December to February. We thus propose a potential distribution for the winter ‘missing generations' that would expand the V. cardui migration cycle to encompass about 15 000 km in latitude, from northernmost Europe to equatorial Africa. In summer, Europe represents the major temporary resource for V. cardui , while January and February show the lowest overall suitability values, and they are potentially the most vulnerable period for the species to suffer yearly bottlenecks. In summary, we demonstrate the potential of the proposed niche modelling strategy to investigate migratory movements of insects.

Role of KCa3.1 Channels in Modulating Ca2+ Oscillations during Glioblastoma Cell Migration and Invasion

Cell migration and invasion in glioblastoma (GBM), the most lethal form of primary brain tumors, are critically dependent on Ca2+ signaling. Increases of [Ca2+]i in GBM cells often result from Ca2+ release from the endoplasmic reticulum (ER), promoted by a variety of agents present in the tumor microenvironment and able to activate the phospholipase C/inositol 1,4,5-trisphosphate PLC/IP3 pathway. The Ca2+ signaling is further strengthened by the Ca2+ influx from the extracellular space through Ca2+ release-activated Ca2+ (CRAC) currents sustained by Orai/STIM channels, meant to replenish the partially depleted ER. Notably, the elevated cytosolic [Ca2+]i activates the intermediate conductance Ca2+-activated K (KCa3.1) channels highly expressed in the plasma membrane of GBM cells, and the resulting K+ efflux hyperpolarizes the cell membrane. This translates to an enhancement of Ca2+ entry through Orai/STIM channels as a result of the increased electromotive (driving) force on Ca2+ influx, ending with the establishment of a recurrent cycle reinforcing the Ca2+ signal. Ca2+ signaling in migrating GBM cells often emerges in the form of intracellular Ca2+ oscillations, instrumental to promote key processes in the migratory cycle. This has suggested that KCa3.1 channels may promote GBM cell migration by inducing or modulating the shape of Ca2+ oscillations. In accordance, we recently built a theoretical model of Ca2+ oscillations incorporating the KCa3.1 channel-dependent dynamics of the membrane potential, and found that the KCa3.1 channel activity could significantly affect the IP3 driven Ca2+ oscillations. Here we review our new theoretical model of Ca2+ oscillations in GBM, upgraded in the light of better knowledge of the KCa3.1 channel kinetics and Ca2+ sensitivity, the dynamics of the Orai/STIM channel modulation, the migration and invasion mechanisms of GBM cells, and their regulation by Ca2+ signals.