Development and functional significance of the pulmonary surfactant system

Relevance. Prevention of the development of postoperative acute respiratory distress syndrome during operations on the descending thoracic aorta increases the effectiveness of therapy. The study of damage to the surfactant complex during ischemia and reperfusion of the lungs is relevant, since it involves the prophylactic use of the surfactant preparation during operations on the descending part of the thoracic aorta, which are characterized by a high risk of postoperative acute respiratory distress syndrome. Objective: to increase the effectiveness of pharmacological and respiratory therapy of acute respiratory distress syndrome, as well as to identify the role of the surfactant system of the lungs in the onset of inflammation against the background of tuberculosis and the development of regeneration mechanisms that affect the course and outcome of the disease. Materials and Methods. The study involved 24 people, including 14 volunteer patients with a diagnosed respiratory disease in an acute course (while the whole group received the drug from the study as an additional therapy). The sample of 14 people was formed solely due to the compliance of these patients with the criteria that were established before the start of the study of the drug, which had postoperative acute respiratory distress syndrome of various origins in their diagnosis. Results and Discussion. For a comprehensive laboratory determination, an algorithm was used that corresponded to the state standard to identify postoperative acute respiratory distress syndrome. For each participant in the experiments, it was proposed to develop a plan of treatment procedures, taking into account individualization and standardization. Conclusion. Some of the resulting data are collected with respect to the surfactant pulmonary system, which is presented in a compactor model format. A number of basic components are reflected here, which are classified according to cellular and non-cellular factors. At the same time, the surfactant substance helps to reduce the pronounced swelling, which can significantly reduce the process of sticking of the alveolar structures during inhalation. All this added up to the normal system of gas metabolism in the lung structures, including the control of the mucociliary system, which acts as a natural stimulator of the function of alveolar macrophages.

The Model of Sugar Metabolism and Exercise Energy Expenditure Based on Fractional Linear Regression Equation

Carbohydrate metabolism can provide energy for human exercise. However, different exercise intensities will consume different amounts of energy. For this reason, the paper uses a fractional linear regression equation to study the characteristics of carbohydrate metabolism and energy consumption of other groups of human beings with the same oxygen consumption during exercise. In addition, the thesis measures energy consumption during exercise and body recovery by analyzing gas metabolism methods. As a result, we found that the sugar, fat metabolism, and energy consumption of heavier volunteers under the same exercise intensity were lower than those of regular weight volunteers. Thus, the fractional linear regression method can help us analyze the relationship between glucose metabolism and exercise energy.

The rationality of physical fitness evaluation index for cardiorespiratory fitness in medical system

Healthy fitness is considered to be closely related to good health. Nowadays, in a modern society, people are less and less active, and high-nutrient intake is increasing. In the face of high-intensity work and life pressure, people’s leisure and entertainment time will increase relatively. When people are deeply aware of the importance of good physical fitness and regular exercise for health. Physical fitness training can bring some positive factors to the body. The purpose of this paper is to study the rationality of fitness assessment indicators for cardiorespiratory fitness in medical systems. In this paper, the cardiopulmonary function and gas metabolism test (K4b2 exercise cardiopulmonary function tester) was carried out by step-by-step incremental load test (CS3000). The subjects wore a breathing mask and a heart rate monitor on a power bicycle for 5 min to measure the gas metabolism in a quiet state. The incremental load motion is then completed on the power bike. At the same time, the three-axis physical activity monitor produced by ActiGraph in the United States was used to test the energy metabolism during exercise and training. The exercise load starts from 25 W, the rotational speed is 60 r/min, each stage increases by 25 W, the load of each stage lasts for 2 min, there is no interval between the levels, and the heart rate, blood pressure and RPE value at the end of each stage are recorded. The experimental results demonstrates that the method has a significant effect on the functional fitness of the elderly with cancer (P < 0.01). The exercise frequency and exercise time have the most significant influence on the fitness of the elderly with cancer (P < 0.001). The muscle strength, flexibility, balance quality and cardiorespiratory strength of the upper and lower limbs were significantly positively correlated with the exercise frequency and each exercise time, but not by the exercise intensity (P > 0.05).

The effect of menstruation on gas metabolism in women

According to Lanz's studies (Zeit f. Geb. u. Gyn., Bd. LXXX1X, H. I) already in quite healthy women, changes in metabolism can be noticed in connection with menstruation, these changes are especially pronounced in sick women, although - in those with mild diseases, what are the initial stages of tbc, disorders of internal secretion and autonomic nervous system.

Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation

The marine roseobacter-clade affiliated cluster (RCA) represents one of the most abundant groups of bacterioplankton in the global oceans, particularly in temperate and sub-polar regions. They play a key role in the biogeochemical cycling of various elements and are important players in oceanic climate-active trace gas metabolism. In contrast to copiotrophic roseobacter counterparts such as Ruegeria pomeroyi DSS-3 and Phaeobacter sp. MED193, RCA bacteria are truly pelagic and have smaller genomes. We have previously shown that RCA bacteria do not appear to encode the PlcP-mediated lipid remodeling pathway, whereby marine heterotrophic bacteria remodel their membrane lipid composition in response to phosphorus (P) stress by substituting membrane glycerophospholipids with alternative glycolipids or betaine lipids. In this study, we report lipidomic analysis of six RCA isolates. In addition to the commonly found glycerophospholipids such as phosphatidylglycerol (PG) and phosphatidylethanolamine (PE), RCA bacteria synthesize a relatively uncommon phospholipid, acylphosphatidylglycerol, which is not found in copiotrophic roseobacters. Instead, like the abundant SAR11 clade, RCA bacteria upregulate ornithine lipid biosynthesis in response to P stress, suggesting a key role of this aminolipid in the adaptation of marine heterotrophs to oceanic nutrient limitation.

Lipidomic analysis of roseobacters of the pelagic RCA cluster and their response to phosphorus limitation

The marine roseobacter-clade Affiliated cluster (RCA) represents one of the most abundant groups of bacterioplankton in the global oceans, particularly in temperate and sub-polar regions. They play a key role in the biogeochemical cycling of various elements and are important players in oceanic climate-active trace gas metabolism. In contrast to copiotrophic roseobacter counterparts such as Ruegeria pomeroyi DSS-3 and Phaeobacter sp. MED193, RCA bacteria are truly pelagic and have smaller genomes. We have previously shown that RCA bacteria do not appear to encode the PlcP-mediated lipid remodelling pathway, whereby marine heterotrophic bacteria remodel their membrane lipid composition in response to phosphorus (P) stress by substituting membrane glycerophospholipids with alternative glycolipids or betaine lipids. In this study, we report lipidomic analysis of six RCA isolates. In addition to the commonly found glycerophospholipids such as phosphatidylglycerol and phosphatidylethanolamine, RCA bacteria synthesise a relatively uncommon phospholipid, acylphosphatidylglycerol, which is not found in copiotrophic roseobacters. Instead, like the abundant SAR11 clade, RCA bacteria upregulate ornithine lipid biosynthesis in response to P stress, suggesting a key role of this aminolipid in the adaptation of marine heterotrophs to oceanic nutrient limitation.

Gas Crosstalk between PFPE–PEG–PFPE Triblock Copolymer Surfactant-Based Microdroplets and Monitoring Bacterial Gas Metabolism with Droplet-Based Microfluidics

The PFPE–PEG–PFPE (Perfluoropolyether-polyethylene glycol-perfluoropolyether) surfactant has been used in droplet-based microfluidics and is known to provide high droplet stability and biocompatibility. Since this surfactant ensures the stability of droplets, droplet-based microfluidic systems have been widely used to encapsulate and analyze various biological components at the single-molecule scale, including viruses, bacteria, nucleic acids and proteins. In this study, we experimentally confirmed that gas crosstalk occurred between droplets formed by fluorinated oil and the PFPE–PEG–PFPE surfactant. E. coli K-12 bacterial cells were encapsulated with Luria–Bertani broth within droplets for the cultivation, and gas crosstalk was identified with neighboring droplets that contain phenol red. Since bacteria produce ammonia gas during its metabolism, penetration of ammonia gas initiates a color change of phenol red-containing droplets. Ammonia gas exchange was also confirmed by reacting ammonium chloride and sodium hydroxide within droplets that encapsulated. Herein, we demonstrate the gas crosstalk issue between droplets when it is formed using the PFPE–PEG–PFPE surfactant and also confirm that the density of droplet barrier has effects on gas crosstalk. Our results also suggest that droplet-based microfluidics can be used for the monitoring of living bacteria by the determination of bacterial metabolites during cultivation.

The Role of Hyperhomocysteinemia in the Development of Changes in the Lungs

Background: In recent years, attention of the scientific community has been drawn to the study of the role of homocysteine in the pathology of diseases in general and the respiratory system in particular. Violations of the synthesis and disposal of homocysteine are the cause of its excessive accumulation in the body, which subsequently leads to damage to all organs and systems. Methods: We conducted this study on 64 white non-linear rats of 6-8 and 24-26 months old rats, which were divided into two control and experimental groups during the experiment. The study of ultrastructural changes in the lungs of rats was performed using an electron microscope. Results: The results of the conducted study show that all the animals of the experimental group, corresponding to 32 (50%) cases, possess conspicuous morphological changes in the structure of the lungs. : The specific features of mature animals were the narrowing of the alveolar space of the part of the alveoli and the increase in the number of macrophages in them. Partial desquamation of capillary endothelial lining and a slight increase of collagen fibers in interalveolar membranes were observed compared to control animals. A characteristic feature for older animals was the growth of connective tissue, predominantly collagen fibers, which led to pneumosclerosis. The thickening of the aerogemic barrier was also detected, and the endothelial lining was intermittent or desquamated. Conclusion: Mature animals of the experimental group were characterized by activation of inflammatory processes, oxidative stress, due to increased production of macrophages and, as a consequence, the launch of the humorous link of immunity, while in old rats were determined by fibrosis, disorders of the trophic and gas metabolism, as well as damage to the endothelium part of lungs.

PL-014 The gaseous metabolism characteristics of overweight adult women during exercise stress test

Objective Overweight was a global public health problem. In recent years, the number of overweight people in China had been increasing. Being overweight had a serious impact on health. 31.1% of overweight people had aggregation of risk factors for cardiovascular metabolic diseases. And overweight people were more likely to suffer from some diseases, such as hypertension, diabetes, dyslipidemia and arthritis. This study compared the gas metabolism index differences between overweight and normal weight women when they did exercise under different load, and summarized gas metabolism characteristics of overweight women, in order to lay the foundation for instructing overweight women to do exercise scientifically, reduce the risk factors of chronic diseases such as cardiovascular disease, and enhance and improve physical fitness and health. Methods Adult women between 20 and 30 years were taken as subjects. After measuring their height and weight, they were divided into normal weight group (BMI=18~23.9kg/m2) and overweight group (BMI＞24kg/m2) according to body mass index (BMI). There were 15 participants in each group. After the baseline test, using modified Bruce treadmill protocol, the air metabolism indexes of two groups were determined by Cortex MetaMax 3B portable gas metabolic analyzer, including oxygen uptake(VO2), minute ventilation(MV), breathing frequency(BF), expiratory end-tidal CO2concentration(ETCO2), expiratory end-tidal O2concentration (ETO2), arterial blood carbon dioxide partial pressure (PaCO2), carbon dioxide output(VCO2), oxygen pulse and maximal voluntary ventilation(MMV), etc. The differences of gas metabolism indexes among resting, exercise, and recovery stages were compared and analyzed. Results (1) Most of indexes such as VO2, VCO2, and MV rose gradually with the load increase during exercise stress test except for ETO2and PaCO2. VO2, PaCO2, VCO2and ETCO2of overweight group were significantly lower than normal weight group during the same load. PaCO2of overweight group at grade 4 was significant lower than normal weight group by 5.6 mmHg (P<0.05). VCO2of overweight group at grade 5 was significant lower than normal weight group by 0.6L/min (P<0.05). ETCO2 of overweight group at grade 3 and 4 were significant lower than normal weight group about 0.5% and 0.6% respectively (P<0.05). (2) During recovery stage, most of indexes decreased gradually, such as MV and BF, while ETO2presented a rising trend. At a certain time during the recovery stage, ETCO2of overweight group was significantly lower than normal weight group (5.3% vs 5.8%), while MMV, MV and oxygen pulse were significantly higher than normal weight group (P<0.05). MMV of overweight group at 2, 3 and 4 minutes were significant lower than normal weight group by1L/min, 1L/min and 0.9L/min, at the same time, MV of overweight group were significant lower than normal weight group by17.8L/min, 20.1L/min and 16.9L/min. The oxygen pulse of overweight group during whole 5 minutes recovery period were significantly higher than normal weight group by 2.7L/min, 3.9L/min, 3.9L/min, 2.9L/min and 2.0L/min. (3) The gaseous metabolism between two groups was significantly different when they did 7.1 and 10.2 METs exercise. Conclusions Although there was no difference in gas metabolism between overweight and normal weight adult women in resting state, the respiratory function of overweight women was weaker than normal weight women during exercise, especially at the intensities of 7.1 and 10.2 METs. During the recovery period after exercise stress test, the recovery rate of gas metabolism in overweight adult women was slower than that of normal weight women.

Method-Induced Differences of Energy Contributions in Women’s Kayaking

Context: Different relative aerobic energy contribution (WAER%) has been reported for the 2 women’s Olympic kayaking disciplines (ie, 200 and 500 m). Purpose: To investigate whether the adopted method of energy calculation influences the value of WAER% during kayaking time trials. Methods: Eleven adolescent female kayakers (age 14 ± 1 y, height 172 ± 4 cm, body mass 65.4 ± 4.2 kg, VO2peak 42.6 ± 4.9 mL·min−1·kg−1, training experience 1.5 ± 0.3 y) volunteered to participate in 1 incremental exercise test and 2 time trials (40 and 120 s) on the kayak ergometer. A portable spirometric system was used to measure gas metabolism. Capillary blood was taken from the ear lobe during and after the tests and analyzed for lactate afterward. The method of modified maximal accumulated oxygen deficit (m-MAOD) and the method based on the fast component of oxygen-uptake off-kinetics (PCr-La-O2) were used to calculate the energy contributions. Results: The anaerobic energy portions from m-MAOD were lower than those from PCr-La-O2 in the 40-s (41.9 ± 8.8 vs 52.8 ± 4.0 kJ, P > .05) and 120-s (64.1 ± 27.9 vs 68.2 ± 10.0 kJ, P > .05) time trials, which induced differences of WAER% between m-MAOD and PCr-La-O2 (36.0% vs 30.0% in 40 s, P > .05; 60.9% vs 57.5% in 120 s, P > .05). Conclusions: The reported different WAER% in women’s Olympic kayaking could be partly attributed to the adopted method of energy calculation (ie, m-MAOD vs PCr-La-O2). A fixed method of energy calculation is recommended during the longitudinal assessment on the relative energy contribution in women’s Olympic kayaking.