scholarly journals Determination of the species of fungi of the genus Fusarium by molecular method

2021 ◽  
pp. 118-124
Author(s):  
L. M. Sokolova ◽  
A. A. Egorova ◽  
A. N. Hovrin

Currently, more than 10,000 species of fungi associated with plants have been found, and it is not surprising that fungal diseases cause a lot of harm. Most species of the genus Fusarium are soil protrophs that live on dead plant remains, in the rhizosphere of plants, on the surface of roots. They cause rot of roots, seeds, fruits, tubers, root crops. All forms of fusariums progress at high temperatures and humidity. The analysis of literary sources and experimental data allowed us to formulate the purpose of our research: “To analyze the species diversity of fungi of the genus Fusarium from the soil, table carrot plants, depending on the ecological and geographical zones of growth.” As a result, using PCR analysis, 13 samples attributed to F. langsethiae were identified; 2 samples of F. ochusrohim; 8 samples of F. roae; 2 of F. sporotrichioides; 1 sample attributed to F. sulmorum, this sample was collected in the Rostov region and the Moscow region. It was also revealed that the diversity of soil and climatic conditions in different zones leads to a change in the species composition of representatives of R. Fusarium: the species composition of fungi of R. Fusarium varies significantly depending on agro-climatic conditions. In wetter and warmer conditions (southern regions), specific species of F. culmorum, F. sporotrichiella, and F. oxysporum dominate. F. heterosporum, F. nivale are less common; F. graminearum species lives on plant residues, on plant roots or in the rhizosphere zone, but practically does not occur in the soil in its pure form. Thus, this direction is promising for the study of fungi of the genus Fusarium, since this microorganism lives in the soil and on plants. It is advisable to identify the pathogen from samples from different regions to significantly increas the representativeness of samples within the regions with further identification of diverse mycelium, thereby creating a scale of diverse mycelium.

2021 ◽  
Vol 258 ◽  
pp. 04042
Author(s):  
Nodirbek Tufliev ◽  
Sherzod Akhmedjanov

Diversity of natural climatic conditions in Central Asia leads to the mass development and spread of cross-border pests, especially locusts, in these provinces. This requires monitoring studies to determine the species composition of endangered locusts that migrate from one state to another in transboundary areas and among them the most major pest species that pose a threat to agricultural crops. Therefore, this article identifies the species composition of locusts found in the border areas of Uzbekistan with neighboring republics of Tajikistan, Kyrgyzstan and Turkmenistan, including harmful species. The results of the use of geographic information system (GIS) technologies in monitoring the spread of locusts in the cross-border areas of Surkhandarya, Kashkadarya, Jizzakh and Namangan provinces are presented.


Author(s):  
А.В. Терешкин ◽  
А.Л. Калмыкова ◽  
Т.А. Андрушко

Вертикальное озеленение с участием различных видов лиан в современных условиях имеет важное эстетическое и санитарно-гигиеническое значение. Особо актуально решение вопросов обогащения флоры городских территорий лианами в степных районах в связи с бедным видовым составом и резким ухудшением экологической ситуации. Объектами исследований являлись 7 видов лиан, различных жизненных форм (однолетние, многолетние), произрастающие в населенных пунктах Саратовской области (Аткарск, Саратов). Цель исследований – изучение эколого-биологических особенностей и мелиоративных свойств лиан и выявление перспектив их использования в вертикальном озеленении селитебных территорий Саратовской области. В ходе исследования видового состава, были выявлены наиболее популярные виды однолетних лиан: ипомея красно-голубая (Ipomea tricolor (L.) Roth) и ипомея пурпурная (Ipomea purpurea (L.) Roth), горошек душистый (Lathyrus edoratus L.), настурция (Tropacolum peregrinum L.) и фасоль огненно-красная (Phaseolus coccineus). Большинство (70%) из них произрастают в местах ограниченного пользования. Изученные виды лиан в исследуемых регионах достигают средних природных показателей (при наличии надлежащего ухода), обладают хорошими показателями жизненного состояния, обильно цветут и плодоносят. При воздействии токсикантами различной концентрации на листовые пластинки лиан установлено их степень устойчивости. Выявлено, что однолетние лианы лучше использовать в декоративных целях, а не в санитарно-гигиенических. Сравнительная оценка однолетних видов с многолетними лианами (девичий виноград пятилисточковый и клематис тангутский) показывает устойчивость многолетних видов (в среднем на 3 балла - 40%). Разработаны варианты декоративных композиций с участием травянистых лиан. По степени декоративности выделены однолетние лианы – Ipomea tricolor, I. purpurea (37 – 41 балл), средней степенью отличаются – Lathyrus edoratus (33 балла), Tropacolum peregrinum (30 баллов) и Phaseolus coccineus (20 – 27 баллов). Преимущество многолетних лиан заключается в их устойчивости к резким изменениям климатических условий (на 40 %) по сравнению с однолетними формами. Поэтому они более предпочтительны для озеленения городской среды. Для усиления декоративного эффекта в сезонном аспекте рекомендуются сочетать расширение видового и формового разнообразия растений (многолетние и однолетние лианы, древесно-кустарниковая, цветочная растительность). Установлено, что природно-климатические условия зоны степи и лесостепи в пределах Саратовской области являются достаточно благоприятными для нормального роста и развития древесно-кустарниковой растительности, в том числе травянистых лиан. Таким образом, обоснованное применение древесных лиан в комплексе с традиционными видами насаждений позволит создать комфортные условия проживания населения, регулировать оптимальный температурный баланс и создавать благоприятные микроклиматические условия. Vertical gardening with different types of vines in modern conditions is important aesthetic and sanitary-hygienic value. It is especially important to address the issues of enrichment of the flora of urban areas with vines in the steppe regions due to poor species composition and a sharp deterioration of the ecological situation. The objects of research were 7 species of lianas, various life forms (annual, perennial), growing in the settlements of the Saratov region (Atkarsk, Saratov). The aim of the research is to study the ecological and biological features and reclamation properties of vines and identify the prospects for their use in vertical gardening residential areas of the Saratov region. In the study, species composition was the most popular species of annual vines: morning glory red-blue (Ipomea tricolor (L.) Roth) and purple morning glory (Ipomea purpurea (L.) Roth), the fragrant pea (Lathyrus edoratus L.), nasturtium (Tropacolum peregrinum L.) and runner beans (Phaseolus coccineus). Most (70%) of them grow in restricted areas. Studied species of vines in the study regions reach average natural performance (with proper care), have good indicators of vital condition, bloom abundantly and bear fruit. When exposed to toxicants of different concentrations on the leaf blades of vines established their degree of stability. It was revealed that the annual vines are better used for decorative purposes, and not in the sanitary-hygienic. A comparative assessment of annual species with perennial vines (maiden grapes and clematis Tangut) shows the stability of perennial species (an average of 3 points-40%). The options and decorative compositions with the participation of herbaceous vines. According to the degree of decoration of the allocated annual vine – Ipomea tricolor, I. purpurea (37 – 41 points), the average degree of differ – Lathyrus edoratus (33 points), Tropacolum peregrinum (30 points) and Phaseolus coccineus (20 to 27 points). The advantage of perennial vines is their resistance to sudden changes in climatic conditions (40 %) compared to annual forms. Therefore, they are more preferable for greening the urban environment. To enhance the decorative effect in the seasonal aspect, it is recommended to combine the expansion of species and form diversity of plants (perennial and annual lianas, tree and shrub, floral vegetation). It is established that the climatic conditions of the steppe and forest-steppe zone within the Saratov region are quite favorable for the normal growth and development of tree and shrub vegetation, including herbaceous lianas. Thus, the reasonable use of wood vines in combination with traditional types of plantings will create comfortable living conditions for the population, regulate the optimal temperature balance and create favorable microclimatic conditions.


2019 ◽  
Vol 49 (4) ◽  
pp. 101-105 ◽  
Author(s):  
G. M. Shkyratova ◽  
B. Z. Bazaron ◽  
T. N. Khamiruev ◽  
S. M. Dashinimaev

The seasonal changes in the skin thickness and structure of the horses’ coat, as signs of adaptation to environmental factors, were studied. The experiment was carried out with the livestock kept in a herd using winter-grazing technology without additional feedings in the climatic conditions of the Trans-Baikal Territory. The objects of the research were adult mares of Zabaikalsky breed of horses of the same age, class and fatness. The studies were carried out in the middle of each season (May, July, October, February). The length of the coat was measured with a caliper, the coat itself with the determination of the ratio of hair (fl uffy hair, heterotype hair and coarse hair) and the thickness of the skin fold were measured in accordance with the approved methodological recommendations. The minimum skin thickness in winter was detected in mares on the back and shoulder blade – 4.3 and 4.4 mm, the maximum – on the side and thigh – 4.5 4.6 mm. When compared with the summer period, the increase on the side was 0.8 mm, whereas on the back, shoulder blade and thigh – 0.4 mm (p ≤ 0,001). In spring, thickening of the skin was noted within 0.1-0.3 mm in the same topographic areas, compared to autumn. The quantitative indicators of the coat changed depending on the season of the year. In winter, the coat contained more fl uffy hair (23.10%), and less coarse hair (68.24%), in summer there was a lower content of fl uffy hair (4.33%), but more coarse hair (94.01%.) Sharp seasonal changes were noted with regard to the length of the hair. The longest hair was found in winter and spring – 4.96 and 4.26 cm, whereas the shortest – in summer and autumn – 0.94 and 1.90 cm, respectively.


Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1017
Author(s):  
Hirohisa Mekata ◽  
Tomohiro Okagawa ◽  
Satoru Konnai ◽  
Takayuki Miyazawa

Bovine foamy virus (BFV) is a member of the foamy virus family in cattle. Information on the epidemiology, transmission routes, and whole-genome sequences of BFV is still limited. To understand the characteristics of BFV, this study included a molecular survey in Japan and the determination of the whole-genome sequences of 30 BFV isolates. A total of 30 (3.4%, 30/884) cattle were infected with BFV according to PCR analysis. Cattle less than 48 months old were scarcely infected with this virus, and older animals had a significantly higher rate of infection. To reveal the possibility of vertical transmission, we additionally surveyed 77 pairs of dams and 3-month-old calves in a farm already confirmed to have BFV. We confirmed that one of the calves born from a dam with BFV was infected. Phylogenetic analyses revealed that a novel genotype was spread in Japan. In conclusion, the prevalence of BFV in Japan is relatively low and three genotypes, including a novel genotype, are spread in Japan.


Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 321
Author(s):  
Maria Orfanoudaki ◽  
Anja Hartmann ◽  
Julia Mayr ◽  
Félix L. Figueroa ◽  
Julia Vega ◽  
...  

This study presents the validation of a high-performance liquid chromatography diode array detector (HPLC-DAD) method for the determination of different mycosporine-like amino acids (MAAs) in the red alga Bostrychia scorpioides. The investigated MAAs, named bostrychines, have only been found in this specific species so far. The developed HPLC-DAD method was successfully applied for the quantification of the major MAAs in Bostrychia scorpioides extracts, collected from four different countries in Europe showing only minor differences between the investigated samples. In the past, several Bostrychia spp. have been reported to include cryptic species, and in some cases such as B. calliptera, B. simpliciuscula, and B. moritziana, the polyphyly was supported by differences in their MAA composition. The uniformity in the MAA composition of the investigated B. scorpioides samples is in agreement with the reported monophyly of this Bostrychia sp.


2020 ◽  
Vol 1006 ◽  
pp. 93-100
Author(s):  
Vadym Nizhnyk ◽  
Yurii Feshchuk ◽  
Volodymyr Borovykov

Based on analysis of appropriate literary sources we established that estimation of fire separation distances was based of two criteria: heat flux and temperature. We proposed to use “ignition temperature of materials” as principal criterion when determining fire separation distances between adjacent construction facilities. Based on the results derived while performing complete factorial we created mathematical model to describe trend of changing fire separation distances depending on caloric power of fire load (Q), openings factor of the external enclosing structures (k) and duration of irradiation (t); moreover, its adequacy was confirmed. Based on linear regression equations we substantiated calculation and tabular method for the determination of fire separation distances for a facility being irradiated which contains combustible or otherwise non-combustible façade and a facility where liquid oil products turn. We developed and proposed general methodology for estimation of fire separation distances between construction facilities by calculation.


2021 ◽  
Vol 55 (2 (255)) ◽  
pp. 172-180
Author(s):  
Iren V. Shahazizyan ◽  
Inessa M. Eloyan ◽  
Ruzanna E. Matevosyan ◽  
Siranoush G. Nanagyulyan

In this work we studied the effect of microscopic soil fungi on some lather samples. The species composition of micromycetes inhabiting the samples under study was revealed, and an assessment of the fungal resistance of the materials under study was given. In the process of work, species of microscopic soil fungi were isolated and identified from the soil. In order to determine the degree of resistance to fungi for the infection of samples, a water-spore suspension was obtained. The leather materials were partially destroyed by microscopic fungi, and mold resistance ranged from 2 to 3 on a 5-point scale.


2020 ◽  
Author(s):  
Asma Jebari ◽  
Jorge Álvaro-Fuentes ◽  
Guillermo Pardo ◽  
María Almagro ◽  
Agustin del Prado

Abstract. Temperate grasslands are of paramount importance in terms of soil organic carbon (SOC) dynamics. Globally, research on SOC dynamics has largely focused on forests, croplands and natural grasslands, while intensively managed grasslands has received much less attention. In this regard, we aimed to improve the prediction of SOC dynamics in managed grasslands under humid temperate regions. In order to do so, we modified and recalibrated the SOC model RothC, originally developed to model the turnover of SOC in arable topsoils, which requires limited amount of readily available input data. The modifications proposed for the RothC are: (1) water content up to saturation conditions in the soil water function of RothC to fit the humid temperate climatic conditions, (2) entry pools that account for particularity of exogenous organic matter (EOM) applied (e.g., ruminant excreta), (3) annual variation in the carbon inputs derived from plant residues considering both above- and below-ground plant residue and rhizodeposits components as well as their quality, and (4) the livestock treading effect (i.e., poaching damage) as a common problem in humid areas with higher annual precipitation. In the paper, we describe the basis of these modifications, carry out a simple sensitivity analysis and validate predictions against data from existing field experiments from four sites in Europe. Model performance showed that modified RothC reasonably captures well the different modifications. However, the model seems to be more sensitive to soil moisture and plant residues modifications than to the other modifications. The applied changes in RothC model could be appropriate to simulate both farm and regional SOC dynamics from managed grassland-based systems under humid temperate conditions.


2020 ◽  
Vol 12 (3) ◽  
pp. 366-372
Author(s):  
Parviz NORMATOV ◽  
◽  
Inom NORMATOV ◽  
Richard ARMSTRONG ◽  
◽  
...  

The research object. The hydrological characteristics of the Gunt and Vanch rivers belonging to the Southwestern and Central climatic zones of the Pamirs and are tributaries of the transboundary Pyanj River are considered. Long-term average values of temperature and precipitation as the main factors affecting the formation of river flows are determined for the period 1944–2016. The state of glaciation in the basins of the respective rivers is estimated. Goal. A comparative assessment of the influence of climatic features and mountain orography on the formation of river flows. Determination of the trend of changes in the climatic conditions of the Gunt and Vanch river basins at different periods of more than 70 years (1944–2016). Research methods. Generalization, systematization of meteorological and hydrological characteristics and determination of their change compared with the base period 1960–1990. Statistical processing of meteorological and hydrological data and establishing a trend of changes in climatic and hydrological characteristics using a differentiated method. Research results. It is shown that the nature of changes in hydrological characteristics, namely the flow of rivers in different climatic zones of the Pamir can differ significantly. On the example of two rivers (Gunt and Vanch) - tributaries of the transboundary Pyanj river, it is shown that the trend of change in the flow of the Gunt river differs from the Vanch river due to the state of glaciation of the river basin. To explain the results the meteorological conditions of the respective river basins and their possible impact on river hydrology were studied. Conclusion. The hydrological characteristics of the Gunt and Vanch rivers in the Pamirs taking into account the meteorological conditions of the respective river basins show that a key factor in improving the state of glaciation and reducing degradation processes, as well as achieving positive trends in the mass balance of glaciers is the presence of sufficient air mass in the glaciation zone. Naturally, this is the key to ensuring a sufficient amount of runoff.


2018 ◽  
Vol 23 ◽  
pp. 120-125
Author(s):  
S. M. Sichkar ◽  
L. H. Velykozhon ◽  
O. V. Dubrovna ◽  
B. V. Morgun

Aim. Determination of the allelic composition of Glu-1 loci in samples of rare wheat species and their hybrids with spring bread wheat. Methods. PCR analysis. Results. In existing collection samples of the rare wheat species the alleles of the a/c loci Glu-A1 were found, while allele b was found only in T.dicoccum, var. volgense (Emmer Kokchetavskaya). An additional amplicon with a length of 450 bp was found in the Glu-A1 locus in the sample (T. dicoccum × Dasypyrum villosum), and in (Ae. ventricosa × T. dicoccum) another one a length of 700 bp in genome B, was found require further research. Among hexaploid wheat, it was revealed genotypes differing in the presence of alleles a and d of the locus Glu-D1. The hybridity of the received forms has been confirmed, as evidenced by the identification of both parent components in the hybrids or alleles of the Glu-D1 locus of bread wheat in hybrids with emmer. Conclusions. Analysis of the allele composition of Glu1 locuses in samples of rare wheat species and their hybrids with bread wheat allowed to select genotypes and hybrid combinations that may be promising for further breeding work. Keywords: Triticum spelta L., T. dicoccum, hybrids, PCR analysis, Glu-1 locus.


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