Increasing azithromycin resistance in Neisseria gonorrhoeae due to NG-MAST 12302 clonal spread in Canada, 2015-2018

Objectives: Azithromycin resistant (AZIR) gonorrhea has been steadily increasing in Canada over the past decade which is cause for alarm as azithromycin (AZI) has been part of the combination therapy recommended by the Canadian Guidelines on Sexually Transmitted Infections (CGSTI) since 2012. Method: Neisseria gonorrhoeae (NG) with AZI MICs ≥ 1 mg/L collected between 2015 and 2018 as part of the Gonococcal Antimicrobial Surveillance Program-Canada underwent antimicrobial susceptibility testing, molecular typing and whole genome sequencing. Regional, demographic and clinical isolation site comparisons were made to aid in our understanding of AZI susceptibility trending. Results: 3,447 NG with AZI MICs ≥ 1 mg/L were identified in Canada, increasing from 6.3% in 2015 to 26.5% of isolates in 2018. Central Canada had the highest proportion rising from 9.2% in 2015 to 31.2% in 2018. 273 different NG-MAST sequence types were identified among these isolates with ST-12302 the most prevalent (50.9%). Whole genome sequencing identified the Neisseria lactamica -like mosaic mtr locus as the mechanism of AZIR in isolates of ST-12302 and isolates genetically similar (differ by ≤ 5 base pairs) designated as the ST-12302 genogroup, accounting for 65.2% of study isolateswhich were originally identified in central Canada but spread to other regions by 2018. Conclusion: Genomic analysis indicated that AZIR in Canadian NG expanded rapidly due to clonal spread of the ST-12302 genogroup. The rapid expansion of this AZIR clonal group in all regions of Canada is of concern. CGSTI are currently under review to address the increase in AZIR in Canada.

A novel method to identify sub-seasonal clustering episodes of extreme precipitation events and their contributions to large accumulation periods

Temporal (serial) clustering of extreme precipitation events on sub-seasonal timescales is a type of compound event. It can cause large precipitation accumulations and lead to floods. We present a novel, count-based procedure to identify episodes of sub-seasonal clustering of extreme precipitation. We introduce two metrics to characterise the prevalence of sub-seasonal clustering episodes and their contribution to large precipitation accumulations. The procedure does not require the investigated variable (here precipitation) to satisfy any specific statistical properties. Applying this procedure to daily precipitation from the ERA5 reanalysis data set, we identify regions where sub-seasonal clustering occurs frequently and contributes substantially to large precipitation accumulations. The regions are the east and northeast of the Asian continent (northeast of China, North and South Korea, Siberia and east of Mongolia), central Canada and south of California, Afghanistan, Pakistan, the southwest of the Iberian Peninsula, and the north of Argentina and south of Bolivia. Our method is robust with respect to the parameters used to define the extreme events (the percentile threshold and the run length) and the length of the sub-seasonal time window (here 2–4 weeks). This procedure could also be used to identify temporal clustering of other variables (e.g. heat waves) and can be applied on different timescales (sub-seasonal to decadal). The code is available at the listed GitHub repository.

Total and Merchantable Volume Equations for 25 Commercial Tree Species Grown in Canada and the Northeastern United States

Accurate estimates of tree bole volume are fundamental to sustainable forest management. Total inside and outside bark and merchantable volume equations were developed for 25 major commercial tree species grown in natural stands in eastern and central Canada and the northeastern United States. Data used to develop these equations was collected from 9647 trees sampled from natural stands across the study area. The number of trees sampled varied among species. Jack pine (Pinus banksiana Lamb.) had the most observations (1648 trees) and American basswood (Tilia americana) and red oak (Quercus rubra L.) had the fewest (28 trees each). Two mathematically consistent volume equations (dimensionally compatible and combined variable) were fitted to inside and outside bark and merchantable tree volume data from these tree species. The final volume equation was selected based on fit statistics, predictive accuracy, and logical consistency. Its predictive accuracy was compared with a volume equation previously developed by Honer. Both (total and merchantable) volume equations were fitted using a nonlinear mixed-effects modelling approach. However, random effects were significant for total volumes for only four tree species. A weight (power function) was used to address heteroscedasticity in the data. The modified form of the dimensionally compatible volume equation outperformed the combined variable volume equation in terms of fit statistics and predictive accuracy and was selected as the total inside and outside bark and merchantable volume equations for all tree species. This equation produced logically consistent estimates of total and merchantable volumes and was more accurate than that previously developed by Honer to estimate volumes for most of the tree species used in this study. This new equation can be used to estimate total inside and outside bark and merchantable volumes of major commercial tree species in eastern and central Canada and the northeastern United States.

Canadian Mapping of Autism-Specific Supports for Postsecondary Students

Background. Many autistic students have a variety of strengths and the desire to succeed in postsecondary education. Nonetheless, most autistic students report not receiving adequate support in postsecondary education to ensure their success. Students also report difficulty in navigating complex institutional systems. We conducted an environmental scan of autism-specific supports (e.g., website information, transition programs, peer mentoring) available to autistic students within Canada’s publicly-funded postsecondary institutions. We also examined distribution of autism-specific supports across institutional type (i.e., university, junior college, technical/vocational) and geographic region.Method. A Boolean search strategy was used to collect data from institutional websites.Results. Of the 258 publicly-funded postsecondary institutions in Canada, only 15 institutions (6%) had at least one support. Of the 15 institutions identified, the most common autism-specific support included information on the institution’s website (67%), followed by transition to university support (47%), social group(s) (33%), peer mentoring (27%), specialist tutoring and support with daily living (20%), transition to employment support (13%), and student-led societies and autistic student advocate (7%). In general, universities and institutions in Central Canada (i.e., Ontario) had a higher number of provisions than expected.Conclusions. There are promising advances with respect to autism-specific supports in post-secondary institutions across Canada. We recommend further research to better understand how students access these supports and more comprehensive evaluations of such supports, specifically informed by collaborations with autistic students.

On nesting

Writer Julietta Singh talks to filmmaker Chase Joynt about their unfolding collaborative work on a feature-length hybrid documentary, The Nest. Taking a majestic home in central Canada as its focus, the documentary looks to architecture as a portal through which to tell unexpected histories of Westward expansion, Indigenous uprising, ecopolitical activism, domestic violence, and the racialization of a nation. Mapping the structural, political, and intimate histories of the house, the film engages archival remnants and historical fabulation to illuminate forgotten feminist pasts and tell linked stories of its transhistorical occupants. The project asks: How can built environments reveal subjugated stories of the past? How are we affected by the historical traces that linger in our dwelling places? How are race, gender, class, sexuality, and physical ability embedded in architecture? And how might we ultimately understand ourselves as artifacts of space and place that are making and telling histories otherwise?

Implications of COVID-19 vaccination and public health countermeasures on SARS-CoV-2 variants of concern in Canada: evidence from a spatial hierarchical cluster analysis

Background: The influence of coronavirus disease-2019 (COVID-19) containment measures on variants of concern (VOC) has been understudied in Canada. Our objective was to identify provinces with disproportionate prevalence of VOC relative to COVID-19 mitigation efforts in provinces and territories in Canada. Methods: We analyzed publicly available provincial- and territorial-level data on the prevalence of VOCs in relation to mitigating factors (summarized in three measures: 1. strength of public health countermeasures: stringency index, 2. how much people moved about outside their homes: mobility index, and 3. vaccine intervention: proportion of Canadian population fully vaccinated). Using spatial agglomerative hierarchical cluster analysis (unsupervised machine learning), the provinces and territories were grouped into clusters by stringency index, mobility index and full vaccine coverage. Kruskal-Wallis test was used to determine the differences in the prevalence of VOC (Alpha, or B.1.1.7, Beta, or B.1.351, Gamma, or P.1, and Delta, or B.1.617.2 variants) between the clusters. Results: Three clusters of vaccine uptake and countermeasures were identified. Cluster 1 consisted of the three Canadian territories, and characterized by higher degree of vaccine deployment and lesser degree of countermeasures. Cluster 2 (located in Central Canada and Atlantic region) was typified by lesser implementation of vaccine deployment and moderate countermeasures. The third cluster was formed by provinces inthe Pacific region, Central Canada, and Prairie region, with moderate vaccine deployment but stronger countermeasures. The overall and variant-specific prevalence were significantly different across the clusters. Interpretation: This study found that implementation of COVID-19 public health measures varied across the provinces and territories. Considering the high prevalence of VOCs in Canada, completing the second dose of COVID-19 vaccine in a timely manner is crucial.

Effect of biofuel waste, urea, deer browsing and vegetation control on Pinus banksiana seedling growth on a dry upland site in central Canada

Ash from biofuels and nitrogen fertilizer are increasingly being used as soil amendments. While this can increase tree growth, it can also increase mammalian grazing and competition with vegetation. We applied moderate amounts (1.5 t ha^-1 y^-1) of ash and 74 kg N ha^-1 y^-1 of urea in each of two years to a well-drained site in southeastern Manitoba, planted with <i>Pinus banksiana</i>. Subplots received deer browsing and/or vegetation control. The ash resulted in an increase in pH in the upper 15m of mineral soil from ca. 5.7 to 6.6, and the urea created short-term spikes in soil inorganic N (NH₄ and NO₃) levels. Urea combined with ash significantly increased seedling relative growth rates in the first two years, with seedlings being largest with urea, with or without ash. However, by the fourth year seedling growth and size did not differ between the amendments. Urea application increased browsing damage to 91 %, but only when vegetation was mowed. Browsing guards resulted in seedlings having 1.6 times greater shoot mass by the end of the fourth growing season. These results suggest that on sandy soils in the dry region of central Canada, <i>P. banksiana</i> may get little benefit from ash applications.

FIRE EVACUATION MODELLING OF A CANADIAN WILDLAND URBAN INTERFACE COMMUNITY

Wildland Urban Interface (WUI) communities are situated at the interface between human development and wildland fuel. In addition to their proximity to susceptible regions, routes of evacuation in WUIs are often limited, posing great risks to these communities in the event of a natural disaster. To begin the research herein, a Canadian WUI community in central Canada was selected as a case study to investigate assembly and evacuation patterns during a fire evacuation to illustrate the complexity of the situation and the current research needs required. First stage simulations of evacuations were performed in the traffic simulation software PTV VISSIM, which extracted useful data including evacuation times and related parameters. Results demonstrated that the predictive power of the software is limited by its ability to incorporate effects of human behaviour and the fire behaviour itself. Thus, extending these findings to include the need for evacuee behaviour and fire dynamics for the formation of a more complete strategic evacuation plans for communities at risk of wildfires.