The effects of substrate morphology by regulating pseudopods formation on cell directional alignment and migration

When cells are cultured on the micro- or nano- structure substrate, filamentous pseudopods are formed at specific locations due to the effects of substrate morphology and local membrane curvature, which provides a powerful method to guide cell migration and neurite orientation. However, it is unclear the effects of substrate surface morphology and initial cell membrane on pseudopod formation and growth. Here, we present a quantitative thermodynamic model to investigate the difficulty of pseudopod formation. Based on the established model, we studied the effects of substrate morphology and the curvature of the initial cell membrane on filamentous pseudopods formation by analyzing the magnitude of an average driving force. We find that the pseudopod-substrate adhesion and the larger curvature radius of the initial cell membrane can facilitate filamentous pseudopods formation due to the smaller minimum resistance energy. Furthermore, our theoretical results seem to show a broad agreement with experimental observations, which implies that these studies would provide useful guidance to control the pseudopods formation on substrate for biomedical applications.

Directed Assembly of Particles for Additive Manufacturing of Particle-Polymer Composites

Particle-polymer dispersions are ubiquitous in additive manufacturing (AM), where they are used as inks to create composite materials with applications to wearable sensors, energy storage materials, and actuation elements. It has been observed that directional alignment of the particle phase in the polymer dispersion can imbue the resulting composite material with enhanced mechanical, electrical, thermal or optical properties. Thus, external field-driven particle alignment during the AM process is one approach to tailoring the properties of composites for end-use applications. This review article provides an overview of externally directed field mechanisms (e.g., electric, magnetic, and acoustic) that are used for particle alignment. Illustrative examples from the AM literature show how these mechanisms are used to create structured composites with unique properties that can only be achieved through alignment. This article closes with a discussion of how particle distribution (i.e., microstructure) affects mechanical properties. A fundamental description of particle phase transport in polymers could lead to the development of AM process control for particle-polymer composite fabrication. This would ultimately create opportunities to explore the fundamental impact that alignment has on particle-polymer composite properties, which opens up the possibility of tailoring these materials for specific applications.

Experimental Investigation on Organic Matter Orientation Characteristics of Terrestrial and Marine Shale in China

As an essential component in shale, OM (organic matter) grains and their arrangements may play essential roles in affecting the anisotropy of the reservoir. However, OM grains are commonly treated as an evenly distributed isotropic medium in current studies, and few works have been done to investigate their detailed arrangement characteristics. In this study, terrestrial and marine shale samples were collected from three different shale plays in China, and the arrangement characteristics of OM grains in each sample were investigated by SEM (scanning electron microscope) image analysis. The results indicate that OM grains in shale are not evenly distributed in isotropic medium, and their directional alignment is pervasive in both marine and terrestrial shale. OM grains in shale tend to subparallel to the bedding section, and their orientation degree and controlling factors differ among different shales. OM grains in samples from terrestrial C-7(Chang-7 Formation) exhibit the strongest directionality in their arrangement, and OM grains in samples from marine LMX (Longmaxi Formation) shale in the Fuling area also exhibit strong directional alignment. While in samples from marine LMX shale in the Baojing area, their directional alignment is much weaker. Shales with high clay content, high TOC (total organic carbon), low thermal maturity, and flat reservoir structure get more OM grains parallel to the bedding section. The biogenetic texture of graptolite in marine LMX shale is the dominating factor leading to the strong directional alignment of the OM grains. However, syncline structure may disorganize the preformed directional alignment and weaken the directionality of the OM grains, which results in the OM arrangement difference between LMX samples from Fuling and Baojing. While the compaction of the layered clay particles is the dominating mechanism leading to the strong directional alignment of the OM grains in terrestrial shale samples from C-7.

Effects of Sea State and Small-Scale Currents on Air-Sea Fluxes in the Northwest Tropical Atlantic Ocean

<p>The Northwest Tropical Atlantic is characterized by the strong North Brazilian Current (NBC), its rings, and numerous mesoscale eddies, which ceaselessly interact with the persistent trade winds and trade cumuli. Near the coast, the ocean stratification is maintained by the Amazon and Orinoco river discharges, which control the vertical mixing and the near-shore circulation dynamics. Breaking waves and swells are ubiquitous under the trade winds, and hence, the wave-induced mixing and wave-mediated air-sea fluxes are expected to modulate the eddy variability and low-level clouds. Our study aims to enhance understanding of the air-sea fluxes mediated by the mesoscale ocean currents and surface waves and evaluate their impacts on the ocean and atmosphere.</p><p>High-resolution ocean model (ROMS) and wave model (WW3) simulations are conducted for the period of the ATOMIC/EUREC4A experiments. The model surface state variables are used to compute offline the air-sea heat and momentum fluxes using the latest COARE v3.6 bulk flux algorithm under various sea state conditions induced by surface waves, ocean currents, and their interaction. The results demonstrate that considering the spatial variability in sea states via wave slope and wave age (e.g., swells and wind-seas) leads to enhanced spatial variability in drag coefficient and wind stress. Comparison to wind stress estimated using the wind-speed dependent formulation, meaning that COARE makes sea state assumptions under given wind, indicates that, at any given time, wind and wave in fact, rarely match those assumptions. The swells (wind-seas) decreases (increases) the sea surface roughness length, drag coefficient, and wind stress by 10-15%. However, we find that the sea state impact on turbulent heat flux is negligible.</p><p>More importantly, we also show that considering the ocean currents in the COARE algorithm yields much stronger spatio-temporal variations in not just the wind stress but also turbulent heat fluxes. The intense and small-scale current fields in this region are associated with the NBC and its rings, smaller mesoscale eddies, and filamentary density fronts associated with the freshwater plumes. The surface currents associated with these small-scale energetic features alter the relative wind speed and thus the air-sea fluxes depending on the directional alignment between the wind and current; the increase (decrease) in both the wind and heat fluxes by ~20% is found with the current and wind are in the opposite (same) direction wind. Moreover, this relative wind effect appears to be reinforced by wave direction as well, also via the directional alignment between waves and currents, since the waves are mainly aligned with the trade wind in this region.</p><p>Further analyses are underway to examining the seasonality of the modulation by the wave-current interaction, quantifying the role of the freshwater distribution, and exploring the time-mean influence on the low-level clouds. The results from the ocean and wave modeling efforts will guide our ongoing fully coupled ocean-atmosphere (and wave) model simulations to quantify their impacts on the atmosphere, including low-level clouds.</p>

Self-organization and information transfer in Antarctic krill swarms

Antarctic krill swarms are one of the largest known animal aggregations. However, despite being the keystone species of the Southern Ocean, little is known about how swarms are formed and maintained, and we lack a detailed understanding of the local interactions between individuals that provide the basis for these swarms. Here we analyzed the trajectories of captive, wild-caught krill in 3D to determine individual level interaction rules and quantify patterns of information flow. Our results suggest krill operate a novel form of collective organization, with measures of information flow and individual movement adjustments expressed most strongly in the vertical dimension, a finding not seen in other swarming species. In addition, local directional alignment with near neighbors, and strong regulation of both direction and speed relative to the positions of groupmates suggest social factors are vital to the formation and maintenance of swarms. This research represents a first step in understanding the fundamentally important swarming behavior of krill.

Stiffness Modulation for a Planar Mobile Cable-Driven Parallel Manipulators via Structural Reconfiguration

Mobile Cable-Driven Parallel Manipulators (m-CDPM) are a sub-class of CDPM with greater-capabilities (antagonistic cable-tensioning and reconfigurability) by virtue of mobility of the base-winches. In past work, we had also explored creation of adjustable spring-stiffness modules, in-line with cables, which decouple cable-stiffness and cable-tensions. All these internal-freedoms allow an m-CDPM to track desired trajectories while equilibrating end-effector wrenches and improving lateral disturbance-rejection. However, parameter and configuration selection is key to unlocking these benefits. To this end, we consider an approach to partition task-execution into a primary (fast) winch-tension control and secondary (slow) reconfiguration and joint-stiffness modulation. This would enable a primary trajectory-tracking task together with secondary task-space stiffness tailoring, using system-reconfiguration and joint-stiffness modulation. In this paper, we limit our scope to feasibility-evaluation to achieve the stiffness modulation as a secondary goal within an offline design-optimization setting (but with an eye towards real-time implementation). These aspects are illustrated in the context of a 3-PRP m-CDPM for tracking a desired trajectory within its wrench-feasible workspace. The secondary-task is the directional-alignment and shaping of the stiffness ellipsoid to shape the disturbance-rejection characteristics along the trajectory. The optimization is solved through constrained minimization of a multi-objective weighted cost function subject to non-linear workspace feasibility, and inequality stiffness and tension constraints.

Business/IT Alignment in Two-Sided Markets

Business/IT alignment can be considered a key challenge in IT governance and becomes especially important in IT-heavy and internet based business models. Recent discussions express the need for a bi-directional paradigm for internet based business models. IT governance frameworks support business/IT alignment but mostly follow a business-driven alignment paradigm. We identify characteristics of internet based business models and use the case of streaming to examine how the IT governance framework COBIT 5 can integrate these characteristics under consideration of a bi-directional business/IT alignment process. We reveal that requirements for streaming business models may not be fully covered by the framework. Based on a structural description of internet based business models and the COBIT 5 Goal Cascade, we explain these specific requirements and propose a possible integration of a bottom-up alignment. With this work we provide guidance in the challenge of business/IT alignment for internet based business models and show pathways for IT governance frameworks to better support a bi-directional alignment.

Bi-Directional Business/IT Alignment

Business/IT (information technology) alignment can be considered as one of the key challenges of information management (IM). A vast majority of studies assumes an uni-directional alignment process that seeks to link the IT strategies to superior business objectives. However, there are plenty of market situations where the ability of an enterprise, or even entire branches, to adjust their business model to new IT developments is crucial to survive among their competitors. Not only under the umbrella of new buzz words like digital transformation and digital transition and their glaring examples such as media streaming, these competitive requirements have recently become more vital. Currently dominating business-driven alignment paradigms are seemingly not capable to accommodate these requirements to a full extent. In this chapter, the concept of bi-directional business/IT alignment is explained and defined by extending a well-known 3-layer model of IM. Furthermore, it is analyzed to which extent different IM models as well as common IT governance frameworks support this paradigm.