Lysozyme Treatment Makes 16S rRNA Amplicon Sequencing Results Less Biased

Background Amplicon sequencing is widely applied in gut bacteria structure analysis. However, the proportion of Gram-positive bacteria may greatly affect the results of microbial community analysis. Lysozyme is an effective agent to extract DNA of Gram-positive bacteria. In this study, we assessed the influence of lysozyme treatment on results of Bactrocere dorsalis rectal bacteria structure. Result The results indicated that the total bacteria content can be significantly increased in lysozyme treated samples. Moreover, rectal bacteria diversity was significantly higher in lysozyme treated samples. A detail analysis revealed that abundance of Gram-positive bacteria significantly increased in samples treated with lysozyme. Conclusion This study indicates that lysozyme treatment before DNA extraction is an effective way to reduce bias in bacteria structure analysis, especially for samples with high proportion of Gram-positive bacteria.

Persoonshaven Urban Housing, Rotterdam

The social housing project at Persoonshaven in the Feijenoord district of Rotterdam in the Netherlands provides an adaptation of a common late 19th-century speculative house type. The changes in its appearance, spatial organization, details and structure result from standardized contemporary Dutch construction techniques and current regulations and spatial standards. The house types and building methods will be described in the context of Martin Steinmann’s characterization of traditionalist design as practiced by the Danish architect Kay Fisker.

Assessment of Genetic Diversity and Population Structure in Pea (Pisum sativum L.) Germplasm based on Morphological Traits and SSR Markers

Background: The study was undertaken to assess the genetic diversity and genetic structure among fifty-five pea accessions using morphological traits and SSR markers. Methods: A total of 55 pea accessions were analyzed using eleven phenotypic traits and twenty SSR markers. The data obtained by morphological and molecular profiling was used for the analysis of genetic diversity and for the estimation of genetic diversity estimates, correlation, principal components analysis and population structure. Result: This study reveals that majority of genetic variation was due to variation within population and were clustered into two distinct groups, which reveals a high admixture within individuals. Accessions viz., VRP-82, VRP-320, VRP-194, VRP-375, EC-97280 and EC-8724, showed great diversity as compared to the other accessions based on both morphological and molecular markers. These accessions may assist in developing and planning breeding strategies aimed to produce new varieties in the future.

Imputation of Sensory Properties Using Deep Learning

Predicting the sensory properties of compounds is challenging, due to the subjective nature of the experimental measurements, which rely on a panel of human participants and are therefore also expensive and time-consuming. We describe the application of a state-of-the-art deep learning method, Alchemite™, to the imputation of sparse physicochemical and sensory data and compare the results with conventional quantitative structure-activity relationship methods and a multi-target graph convolutional neural network. The imputation model achieved a substantially higher accuracy of prediction, with improvements in R2 between 0.26 and 0.45 over the next best method for each sensory property. We also demonstrate that robust uncertainty estimates generated by the imputation model enable the most accurate predictions to be identified and that imputation also more accurately predicts activity cliffs, where small changes in compound structure result in large changes in sensory properties. In combination, these results demonstrate that use of imputation, based on data from less-expensive, early experiments enables better selection of compounds for more costly studies, saving experimental time and resources.

Code Compliance in Reinforce Concrete Design: A Comparative Study of USA Code (ACI) and Chinese Code (GB)

The structural engineering codes dictate the design criteria of the facility. Given that different countries use different parameters to design a facility, different codes are followed. Current code comparison focus on the clauses analysis in the code gives theoretical guideline. When the US facility design team needs to make a decision to follow which code to in China, the current study cannot provide a business decision input. This study evaluates the design process to illustrate when and where the codes will be applied and calculated through a design process where the loading and coefficient factors of different codes are analyzed. Software programs built-in codes are then used to design the structure to obtain the structure result in terms of volume of the concrete and weight of the rebar being calculated. The study also presents a case study and calculates that the United States code uses 8–10% more rebar compared to the Chinese code. The study result can be a reference for the project management team who has to make a business decision over which code to be followed at what cost. The paper also identifies the choice of the seismic coefficient factor has a significant impact on the usage of the rebar and might be justified for the future study.

Free Boundary Regularity for Almost Every Solution to the Signorini Problem

We investigate the regularity of the free boundary for the Signorini problem in $${\mathbb {R}}^{n+1}$$ R n + 1 . It is known that regular points are $$(n-1)$$ ( n - 1 ) -dimensional and $$C^\infty $$ C ∞ . However, even for $$C^\infty $$ C ∞ obstacles $$\varphi $$ φ , the set of non-regular (or degenerate) points could be very large—e.g. with infinite $${\mathcal {H}}^{n-1}$$ H n - 1 measure. The only two assumptions under which a nice structure result for degenerate points has been established are when $$\varphi $$ φ is analytic, and when $$\Delta \varphi < 0$$ Δ φ < 0 . However, even in these cases, the set of degenerate points is in general $$(n-1)$$ ( n - 1 ) -dimensional—as large as the set of regular points. In this work, we show for the first time that, “usually”, the set of degenerate points is small. Namely, we prove that, given any $$C^\infty $$ C ∞ obstacle, for almost every solution the non-regular part of the free boundary is at most $$(n-2)$$ ( n - 2 ) -dimensional. This is the first result in this direction for the Signorini problem. Furthermore, we prove analogous results for the obstacle problem for the fractional Laplacian $$(-\Delta )^s$$ ( - Δ ) s , and for the parabolic Signorini problem. In the parabolic Signorini problem, our main result establishes that the non-regular part of the free boundary is $$(n-1-\alpha _\circ )$$ ( n - 1 - α ∘ ) -dimensional for almost all times t, for some $$\alpha _\circ > 0$$ α ∘ > 0 . Finally, we construct some new examples of free boundaries with degenerate points.

New Mononuclear Complex of Europium(III) and Benzoic Acid: From Synthesis and Crystal Structure Solution to Luminescence Emission

This study presents a general method which can be used for the synthesis of mononuclear complexes with europium(III) and organic ligands with carboxylic groups. It describes the procedure for preparing a new mononuclear coordination complex with europium(III) and carboxylate ligands sourced from benzoic acid. The construction of mononuclear complexes with a coordination sphere saturated in carboxylic ligands must go through the preparation and purification of a europium(III) intermediate complex that presents a coordination sphere with anions that will be later exchanged for carboxylic groups and finally precipitated as a solvent-free or anion-free complex within the coordination sphere. The detailed synthesis procedure for powders of a new complex, as well as studies of its structural composition at each phase and luminescent properties, are detailed in this study. Analytical and spectroscopic data reveal the formation of a new mononuclear complex of the general formula [Eu(OOCC6H5)3·(HOOCC6H5)2]. The crystal structure of the Eu(III) complex was solved using X-ray powder diffraction data and EXPO2014 software, and the crystal structure result was deposited in the CCDC service with number 19771999.

Association of Thyroid Peroxidase Gene polymorphisms and serum anti-TPO levels in Egyptian Patients with Autoimmune Hypothyroidism

Background: Thyroid peroxidase (TPO) gene mutation leads to change in enzyme built structure result in the anti-TPO autoantibodies production that may cause thyroid destruction. Aim: to evaluate the association of three single nucleotide polymorphisms (SNPs) of the TPO gene and anti TPO- levels in Egyptian patients with autoimmune hypothyroidism and correlate them with the disease severity. Methods: Two hundred patients with newly discovered autoimmune hypothyroidism were included in the study (100 with subclinical hypothyroidism and 100 of them with overt hypothyroidism) and100 healthy individuals as a control group were genotyped by PCR-REFLP. Results: The TT genotype of rs2071400 C/T and the T allele were significantly more frequent in patients with subclinical hypothyroidism and overt hypothyroidism than in control group. But there were no significant difference in the TT genotype and T allele between subclinical and overt hypothyroidism patient. As regards TPO rs732609 A/C polymorphism, the CC genotype of rs732609 A/C and the C allele were significantly increased in patients with subclinical hypothyroidism and overt hypothyroidism than in controls. There was significant difference in the CC genotype and C allele between subclinical and overt hypothyroidism patients. Conclusion: We found an association of rs2071400 C/T and rs732609A/C polymorphisms with autoimmune hypothyroidism and correlated anti-TPO levels with different genotypes in hypothyroid patients. Also we found an association of rs732609A/C polymorphism with the disease severity.

Wage remuneration and benefits received related to the quality of working life received in the Northeast of the state of Guanajuato, case; Victoria, Tierra Blanca, Santa Catarina, Xichú and Atarjea

This study shows the perception of workers in the municipalities of Atarjea, Tierra Blanca, Santa Catarina, Xichú and Victoria who make up the Northeast of the State of Guanajuato, regarding the current wage structure, result of exploratory quantitative research carried out by students and teachers from the Universidad Tecnológico de Norte de Guanajuato, Campus Victoria. Instrument Design: San Elias Rabbit Carla Michelle, Mejía Rodríguez Edith Magali.