Staphylococcus sciuri SAT-17 improved the growth of salt stressed maize (Zea mays L.) by modulated expression of stress responsive genes and anti-oxidative defence mechanisms

Plant growth promoting rhizobacteria (PGPRs), being the chief components of rhizosphere microbiota, are highly beneficial for plant growth and production. PGPR mediated salt stress tolerance is an intricate process which is governed by plant- bacterial interactions at molecular level. In an earlier study, positive impact of Staphylococcus scuiri SAT-17 inoculation on maize growth and physiology, under saline conditions, has been reported. To further elucidate this interaction at molecular level, salt tolerant and sensitive (FH-988 and FH-1137) maize genotypes were raised with or without inoculation of SAT-17 in the absence or presence of 120 mM NaCl. Expression analysis of various salt responsive genes (NHX1, H+-PPase, SOS1, HKT1, Cat1 and APX1) was carried out. Nutrient acquisition and translocation patterns along with few biochemical parameters were also studied. The results indicated that Na+ vacuolar sequestration and enhanced antioxidant enzyme activities might have accounted for the better salt tolerance potential of genotype FH-988 under salt stress. In contrast, genotype FH-1137 exhibited reduced Na+ vacuolar sequestration and antioxidant enzyme activities, thereby resulting in severe Na+ toxicity and oxidative damage under salt stress. Inoculation of SAT-17 ameliorated the salinity damage by maintaining optimum nutrient root-shoot translocation which in turn resulted in better Na+ homeostasis and reactive oxygen species scavenging. The results highlighted the contribution of several ion transporters, SOS pathway and antioxidant machinery in imparting salt stress tolerance in maize. The findings can be useful for devising strategies for cultivation of salt sensitive maize genotypes in saline areas thereby contributing in sustainable agricultural development.

Responses to Cadmium in Early-Diverging Streptophytes (Charophytes and Bryophytes): Current Views and Potential Applications

Several transition metals are essential for plant growth and development, as they are involved in various fundamental metabolic functions. By contrast, cadmium (Cd) is a metal that can prove extremely toxic for plants and other organisms in a dose-dependent manner. Charophytes and bryophytes are early-diverging streptophytes widely employed for biomonitoring purposes, as they are able to cope with high concentrations of toxic metal(loid)s without showing any apparent heavy damage. In this review, we will deal with different mechanisms that charophytes and bryophytes have evolved to respond to Cd at a cellular level. Particular attention will be addressed to strategies involving Cd vacuolar sequestration and cell wall immobilization, focusing on specific mechanisms that help achieve detoxification. Understanding the effects of metal(loid) pollution and accumulation on the morpho-physiological traits of charophytes and bryophytes can be in fact fundamental for optimizing their use as phytomonitors and/or phytoremediators.

Biochemical and molecular characterisations of salt tolerance components in rice varieties tolerant and sensitive to NaCl: the relevance of Na+ exclusion in salt tolerance in the species

Soil salinisation is a major abiotic stress in agriculture, and is especially a concern for rice production because among cereal crops, rice is the most salt-sensitive. However, the production of rice must be increased substantially by the year 2050 to meet the demand of the ever growing population. Hence, understanding the biochemical events determining salt tolerance in rice is highly desirable so that the trait can be introduced in cultivars of interest through biotechnological intervention. In this context, an initial study on NaCl response in four Indica rice varieties showed a lower uptake of Na+ in the salt-tolerant Nona Bokra and Pokkali than in the salt-sensitive IR64 and IR29, indicating Na+ exclusion as a primary requirement of salt tolerance in the species. This was also supported by the following features in the salt-tolerant, but not in the -sensitive varieties: (1) highly significant NaCl-induced increase in the activity of PM-H+ATPase, (2) a high constitutive level and NaCl-induced threonine phosphorylation of PM-H+ATPase, necessary to promote its activity, (3) a high constitutive expression of 14-3-3 protein that makes PM-H+ATPase active by binding with the phosphorylated threonine at the C-terminal end, (4) a high constitutive and NaCl-induced expression of SOS1 in roots, and (5) significant NaCl-induced expression of OsCIPK 24, a SOS2 that phosphorylates SOS1. The vacuolar sequestration of Na+ in seedlings was not reflected from the expression pattern of NHX1/NHX1 in response to NaCl. NaCl-induced downregulation of expression of HKTs in roots of Nona Bokra, but upregulation in Pokkali also indicates that their role in salt tolerance in rice could be cultivar specific. The study indicates that consideration of increasing exclusion of Na+ by enhancing the efficiency of SOS1/PM-H+ATPase Na+ exclusion module could be an important aspect in attempting to increase salt tolerance in the rice varieties or cultivars of interest.

Morpho-physiological and gene expression responses of wheat by Aegilops cylindrica amphidiploids to salt stress

Aegilops cylindrica Host is one of the most salt-tolerant species in the Triticeae tribe. Amphidiploid plants derived from hybridization of ‘Roshan’ × Aegilops cylindrica and ‘Chinese Spring’ × Ae. cylindrica genotypes contrasting in salt tolerance were assessed for their morpho-physiological responses and the expression patterns of two genes related to ion homeostasis under 250 mM NaCl. Results showed that salt stress caused significant declines in both their morphological and phenological traits. Moreover, salt stress reduced not only their chlorophyll content but also their root and shoot K contents and K/Na ratios, while it led to significant enhancements in the remaining traits. Similar to Ae. cylindrica, the amphidiploids subjected to salt stress exhibited significantly higher H2O2 levels, root and shoot K contents, and root and shoot K/Na ratios accompanied by lower root and shoot Na contents and MDA concentrations when compared with the same traits in the wheat parents. Quantitative Real-Time PCR showed significant differential expression patterns of the NHX1 and HKT1;5 genes between the amphidiploids and their parents. The transcript level of HKT1;5 was found to be higher in the roots than in the shoots of both the amphidiploids and Ae. cylindrica while NHX1 exhibited a higher expression in the shoot tissues. The consistency of these data provides compelling support for the hypothesis that active exclusion of Na from the roots and elevated vacuolar sequestration of Na in the leaves might explain the declining Na levels in the shoots and roots of both the amphidiploids and Ae. cylindrica relative to those measured in wheat parents. It is concluded that the hybridized amphiploids are potentially valuable resources for salt improvement in bread wheat through the backcrossing approach.

Synergy between the anthocyanin and RDR6/SGS3/DCL4 siRNA pathways expose hidden features of Arabidopsis carbon metabolism

Anthocyanin pigments furnish a powerful visual output of the stress and metabolic status of Arabidopsis thaliana plants. Essential for pigment accumulation is TRANSPARENT TESTA19 (TT19), a glutathione S-transferase proposed to bind and stabilize anthocyanins, participating in their vacuolar sequestration, a function conserved across the flowering plants. Here, we report the identification of genetic suppressors that result in anthocyanin accumulation in the absence of TT19. We show that mutations in RDR6, SGS3, or DCL4 suppress the anthocyanin defect of tt19 by pushing carbon towards flavonoid biosynthesis. This effect is not unique to tt19 and extends to at least one other anthocyanin pathway gene mutant. This synergy between mutations in components of the RDR6-SGS3-DCL4 siRNA system and the flavonoid pathway reveals genetic/epigenetic mechanisms regulating metabolic fluxes.