scholarly journals ANTI-INFLUENZA ACTIVITY OF PLANT EXTRACTS OF THE LAMIACEAE FAMILY

2021 ◽  
pp. 181-190
Author(s):  
Mariya Anatol'yevna Protsenko ◽  
Natal'ya Alekseyevna Mazurkova ◽  
Ekaterina Igorevna Filippova ◽  
Tat'yana Abdulkhailovna Kukushkina ◽  
Irina Evgen'yevna Lobanova ◽  
...  

In this work, it was revealed that water and ethanol extracts of the plants Nepeta cataria, Nepeta sibirica, Scutellaria baicalensis, Hyssopus officinalis, Betonica officinalis, and water extracts of Dracocephalum moldavica, Glechoma hederacea, Mentha arvensis, Prunella vulgaris, Melissa officinalis ethanol extracts of Mentha piperita, Mentha crispa, Origanum vulgare, Hyssopus officinalis, Salvia verticillata showed antiviral activity against the influenza virus subtype H5N1. Aqueous extracts of Nepeta cataria and Glechoma hederacea (NI 3.75) showed the highest antiviral effect against the H5N1 subtype. It was revealed the antiviral activity against influenza virus subtype H3N2 of aqueous and ethanol extracts of Scutellaria baicalensis, Mentha piperita, Mentha arvensis, Mentha crispa, aqueous extract of Dracocephalum moldavica and ethanol extracts of Glechoma hederacea, Origanum vulgare, Prunella vulgaris, Hyssopus officinalis, Betonica officinalis, Salvia verticillata. Ethanolic extract of Betonica officinalis (NI 4.25) showed the highest virus neutralizing activity against the H3N2 subtype. It was carried out chemical analysis of the aerial parts of plants of the Lamiaceae family. It was shown that the highest content of flavonols was observed in Dracocephalum nutans (4.47±0.04%), the highest content of tannins was found in Mentha arvensis (17.62 ± 0.78%), and the highest content catechins were found in Nepeta cataria (0.43 ± 0.007%). Thus, plant extracts of the Lamiaceae family are promising sources for further studies to develop new antiviral drugs.

2009 ◽  
Vol 429 (1) ◽  
pp. 538-540 ◽  
Author(s):  
D. V. Kurilov ◽  
E. B. Kirichenko ◽  
G. F. Bidyukova ◽  
L. S. Olekhnovich ◽  
Luu Dam Ku

1979 ◽  
Vol 34 (3-4) ◽  
pp. 200-209 ◽  
Author(s):  
Gudrun Frey-Schröder ◽  
Wolfgang Barz

Abstract Peroxidases from several plants, including horseradish peroxidase, were capable of converting flavonols to the corresponding 2,3-dihydroxyflavanones in presence of H2O2 . Contrastingly, protein extracts from Mentha piperita plants and Mentha arvensis cell suspension cultures perform ed the same enzymatic step in absence of H2O2 , but only with quercetin, not with kaempferol. H2O2-independent, quercetin converting enzymes were isolated and purified from these extracts, and they could be classified in two groups according to the extent of stimulation of the enzyme reaction by H2O2 . Enzymes from group I were stimulated by exogenous H2O2 , and they resembled horse­ radish peroxidase in several aspects. They possessed IAA oxidase activity, but quercetin was the preferred substrate. Enzymes from group II from the plants appeared to be a distinctly different set of enzymes. They were not stimulated by H2O2 , but required molecular oxygen and converted only 3,3′,4′-trihydroxyflavones under aerobic conditions. Also, they showed no Soret-bands and possessed no IAA oxidase activity. These proteins appear to be a new class of enzymes participating in the first step of flavonol degradation in plants.


Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 59 ◽  
Author(s):  
Danuta Kalemba ◽  
Agnieszka Synowiec

This review article discusses the active constituents and potential of two menthol mint oils, Mentha piperita (MPEO) and Mentha arvensis (MAEO), as natural sources for botanical pesticides. The biological activities of these menthol mint oils, which can be useful in agriculture, have been broadly researched, especially toward phytotoxic microorganisms. To a lesser extent, the insecticidal and herbicidal activities of mint EOs have also been studied. It is apparent that the prospect of using menthol mint oils in agriculture is increasing in popularity. A number of investigations showed that the in vitro efficacy of MPEO and MAEO, as well as that of their main constituent, menthol, is pronounced. The results of in vitro research are useful for choosing EOs for further investigations. However, it is clear that in situ experiments are crucial and should be more extensively developed. At the same time, known techniques are to be applied to this area and new methods should be worked out, aiming at the improvement of EOs’ pesticidal efficacy and cost-effectiveness, for future implementation in agricultural pest control.


2020 ◽  
Vol 15 (1) ◽  
pp. 511-521 ◽  
Author(s):  
Soňa Felšöciová ◽  
Nenad Vukovic ◽  
Paweł Jeżowski ◽  
Miroslava Kačániová

AbstractPhytopathogenic fungi have been responsible for considerable economic losses in vineyards, and therefore, more attention should be paid to the development and implementation of preventative treatment that is environmentally friendly. The aim of this study was to evaluate the antifungal activity of ten essential oils (EOs) (viz. Lavandula angustifolia Mill., Carum carvi L., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Satureja hortensis L., Origanum vulgare L., Pimpinella anisum L. and Rosmarinus officinalis L.). For the antifungal activity evaluation against Penicillium brevicompactum, P. citrinum, P. crustosum, P. expansum, P. funiculosum, P. glabrum, P. chrysogenum, P. oxalicum, P. polonicum and Talaromyces purpurogenus a disc diffusion method was used. The ten EOs exhibited different antifungal properties. Three tested EOs (Carum carvi L., Satureja hortensis L. and Pimpinella anisum L.) at concentrations of 0.75, 0.50, 0.25 and 0.125 µL/mL showed antifungal activity, inhibiting the mycelial growth. The Origanum vulgare L. EOs exhibited a lower level of inhibition. Overall, Lavandula angustifolia Mill., Pinus mugo var. pumilio, Mentha piperita L., Foeniculum vulgare L., Pinus sylvestris L., Satureja hortensis L., Pimpinella anisum L. and Rosmarinus officinalis L. were effective as fungicidal agents but their efficiency varied between the strains of fungi. Carum carvi L. showed strong antifungal activity against all tested strains at both full strength and reduced concentrations. These EOs could be considered as potential sources of antifungal compounds for treating plant fungal diseases.


Author(s):  
Christian Andrei Chacín Zambrano

Colombia por su privilegiada ubicación geográfica y gran variedad de biomas y ecosistemas, ofrece una rica diversidad en flora estimada en más de 45.000 especies, equivalente al 10% del total de los géneros que existen a nivel mundial1. Además, nuestra biodiversidad nos lleva a ostentar el primer lugar en variedades de orquídeas, con 4.010 especies, y a su vez el tercero en variedades de palma, con 231 especies2.  La distribución de la riqueza vegetal en nuestro país muestra la región andina como la más favorecida, seguida por el chocó y la amazonia3. Dentro de la región andina, Santander es uno de los departamentos con mayor número de ecosistemas, gracias a su geomorfología, siendo potencia en la actividad agroforestal la cual ha sido vital en el desarrollo de Santander, donde indudablemente su fortaleza se encuentra en los cultivos permanentes, con un total sembrado de 171. 845 hectáreas de los cuales la caña, la mora y la piña aportan un 16% total4. Sin embargo, la ejecución de políticas inadecuadas de ocupación y utilización del territorio, la deforestación, la sobreexplotación y demás métodos para la producción masiva ha ido deteriorando poco a poco los hábitats poniendo en peligro muchas de las especies nativas de la región por falta de conocimiento al llevar a cabo sistemas agroforestales sostenibles5. Es por ello que, siendo conscientes de las necesidades de la región por salvaguardar los recursos genéticos, se estableció un banco de germoplasma con el fin de preservar las especies propias y que por años han sido un motor en el sector productivo agropecuario para el Departamento de Santander como lo son la Piña oro miel (Ananas sativus (lindl) schult), la guayaba silvestre (Psidium guajava l.),  la mora de castilla (Rubus glaucus), la caña de azúcar (Saccharum officinarum), frijol arbustivo (Phaseolus vulgaris) (y aromáticas tales como Orégano (Origanum vulgare), Mentha Piperita (Mentha x piperita), utilizando la técnica del cultivo in vitro vegetal, para ello se estandarizó  un método de desinfección teniendo en cuenta el tipo de desinfectante, las concentraciones y tiempos de exposición con el explante, realizando mediciones de porcentaje de oxidación, porcentaje de explantes prósperos y porcentajes de explantes contaminados; así mismo, se desarrolló la fase de establecimiento de cada uno de los cultivares a partir de diferentes formulaciones en medios de cultivos teniendo en cuenta los tiempos de formación de brotes, el porcentaje de germinación entre otros, para finalizar con el establecimiento de la fase de multiplicación de los explantes, donde se evaluaron variables tales altura de la plántula, número de hojas, tamaño de la raíz y la relación de multiplicación. Dentro de los resultados generados se obtuvo protocolos para el proceso de desinfección, las formulaciones de los medios de cultivo para cada uno de las especies en su fase de establecimiento y multiplicación y a su vez, una relación de multiplicación de 1:20, es decir que a partir de un explante, se alcanzó la multiplicación de 20 plántulas.Cómo citar este resumen: Chacín Zambrano C A, La Biotecnología Vegetal Como Herramienta Para La Conservación De La Biodiversidad, BUCARAMANGA, COLOMBIA. Innovaciencia facultad cienc. exactas fis. naturales. 2017; 5(1).


Author(s):  
É. B. Héthelyi ◽  
L. Gy. Szabó ◽  
K. Korány

In the temperate zone live about 150 species of the Nepeta genus. Our investigations covered the examinations of the volatile oil containing species of the genus endemic in Hungary, Nepeta cataria and Nepeta parviflora. Latter is a relict of the ancient steppe-flora and endemic in Hungary as well. Phytochemical examination of the volatile oil containing plant material has also been carried out. Catnip growing in the Botanical Garden of PTE Department of Botany contained 0,67% volatile oil in May and 0,14% in November. Chemical character of the volatile oils were measured by gas chromatography/mass spectrometry and citronellol, citral-A, citral-B and geraniol components were identified. The composition of the oil of November samples shifted towards citronellol (65%). In both samples insecticide and repellent activity bearing compounds (+)-cis-p-menthane-3,8-diol, and (—)-trans-p-menthane-3,8-diol in 2-2.5 and 4-4.5% amount have been found. The catnip sample deriving from Germany contained a small amount of anetol, citronellol, neral, geraniol and geranial (6-13%), and possibly two isomers of nepetalactone in 23-31%. The Nepeta parviflora endemic in the Nagyvolgy valley near Nagykaracsony consisted of the same compounds in the investigated years (1998-2000). Its limonene, methyl chavicol, b-cariophyllene, b-selinene, b-cubebene, davanone, germacrene-D constituents have been identified. In the year 2000 different GC % of these compounds were detected in the different organs of the plants. The closely related species Nepeta cataria var. citriodora contained 83% citral, and the N. glechoma (= Glechoma hederacea) contained 41% a-cubebene, 20% patchoulenol, 7,7% spathulenol respectively. These compounds were identified by gas chromatography and gas chromatography / mass spectrometry.  


Author(s):  
Lela Lailatul Khumaisah ◽  
Lela Mukmilah Yuningsih ◽  
Asep Kadarohman

The chemical content in essential oils have activities on microbes, biopesticides, pathogenic vector insects in humans and animals, including Mentha arvensis and Mentha piperita (mint plants) which have antifertility and antibacterial effects, Vetiveria zizanoides (vetiver root) as insects repellent, Cymbopogon nardus (lemongrass) has an activity against plant pathogenic bacteria and antifungals, and Rosmarinus officinalis (rosemary) has locomotor activity of mice and antibacterial. Essential oils are effective against target organisms, compatible, safe and non-toxic, so they have enormous potential to be developed as biopesticides in controlling rice pests. On the other hand, pests and diseases are problems that always disturb rice farmers, including in Sukaresmi and Kebon Pedes villages, Sukabumi. Therefore, the use of essential oil plants has been carried out to control rice mouse pests. This has been started from the preparation of seeds to process of planting the four types of essential oils (mint, lemongrass, and rosemary) in the fields as intercrops and the procurement of essential oil distillation sets. Based on observations, the growth and development of essential oil plants as intercropping plants on rice runs normally and its impact on rice pests, especially in mice, is quite significant with not found rats around the planting area.   Keywords: Mint, lemongrass, rosemary, essential oil, biopesticide


2021 ◽  
Vol 2 (5) ◽  
pp. 1405-1415
Author(s):  
Kellvin Costa Maciel ◽  
Nayane Monalys Silva De Lima ◽  
Cícero Jádson Da Costa ◽  
Carlos Alberto Mendes Da Silva Filho ◽  
Josenildo Severino De Vasconcelos ◽  
...  

RESUMO Introdução. As parasitoses representam um grave problema de saúde pública, nas comunidades mais carentes. Rotineiramente a comida acaba sendo veículo de disseminação de microrganismos como, Entamoeba sp e Giardia sp, também tem destaque a não acessibilidade a tratamentos medicamentosos.  Buscou-se analisar a atividade antiparasitária e toxicidade dos extratos: Bidens pilosa, Cucurbita sp , Eucalyptus globulus, Mentha piperita L., Ocimum gratissimum, Petroselinum crispum, Allium sativum, Coriadrum sativum, Dysphania ambrosioide, Punica granatum. frente aos cistos da Giardia sp e Entamoeba sp, como potenciais antiprotozoários. Métodos Foram produzidos extratos brutos secos, hidro alcoólicos, por método maceração e posterior secagem. Os cistos de Ameba sp. e Giárdia sp., foram expostos em soluções dos extratos nas concentrações de 100%, 50%, 25% e 12,5%, com uma proporção de amostra/extrato (1:10). Foi utilizado a técnica de Artemia salina para observação da toxicidade dos mesmos. Resultados Os extratos de Eucalyptus globulus, Mentha crispa, Allium sativum, Coriadrum sativum e Punica granatum, demonstraram maior espectro de ação frente aos microrganismos. As principais alterações, evidenciadas nos cistos, foram retração da membrana e lise celular com extravasamento do citosol.  Observou-se que a maioria dos extratos demonstraram-se praticamente atóxicos. Conclusão As plantas medicinais devem ser estudadas adotando ensaios in vitro e in vivo, para observar a ação antiprotozoária em ambas as situações. O estudo revelou um potencial   para a produção de produtos com fins terapêuticos antiparasitários.   ABSTRACT Introduction. Parasitosis represent a serious public health problem in poor communities. Routinely, food ends up being a vehicle for the dissemination of microorganisms such as Entamoeba sp and Giardia sp.  We sought to analyze the antiparasitic activity and toxicity of the extracts: Bidens pilosa, Cucurbita sp, Eucalyptus globulus, Mentha piperita L., Ocimum gratissimum, Petroselinum crispum, Allium sativum, Coriadrum sativum, Dysphania ambrosioide, Punica granatum. against Giardia sp and Entamoeba sp cysts, as potential antiprotozoal agents. Methods Dry, hydroalcoholic crude extracts were produced by maceration method and subsequent drying. Ameba sp. and Giardia sp. cysts were exposed in solutions of the extracts at concentrations of 100%, 50%, 25% and 12.5%, with a sample/extract ratio (1:10). The Artemia salina technique was used to observe their toxicity. Results The extracts of Eucalyptus globulus, Mentha crispa, Allium sativum, Coriadrum sativum and Punica granatum, demonstrated a greater spectrum of action against microorganisms. The main alterations, evidenced in the cysts, were membrane retraction and cell lysis with cytosol extravasation.  It was observed that most of the extracts were practically non-toxic. Conclusion Medicinal plants should be studied adopting in vitro and in vivo assays, to observe the antiprotozoal action in both situations. The study revealed a potential for the production of products with antiparasitic therapeutic purposes.  


2006 ◽  
Vol 30 (4) ◽  
pp. 731-738 ◽  
Author(s):  
Marcelo Cláudio Pereira ◽  
Georgia Rocha Vilela ◽  
Lívia Martinez Abreu Soares Costa ◽  
Reginaldo Ferreira da Silva ◽  
Anderson Felicori Fernandes ◽  
...  

Objetivou-se com este trabalho avaliar os efeitos inibitórios, "in vitro", de óleos essenciais dos condimentos, alecrim (Rosmarinus officinalis L.), cebola (Allium cepa L.), manjericão (Ocimum basilicum L.), menta (Mentha piperita L.) e orégano (Origanum vulgare L.), sobre o desenvolvimento de fungos. Os óleos foram extraídos pela técnica de arraste a vapor e testados nas concentrações de 500; 1000; 1500 e 2000 mg/mL-1. Como culturas de teste foram utilizados os fungos Fusarium sp.; Aspergillus ochraceus Wilhelm.; Aspergillus flavus Link e Aspergillus niger van Tieghem obtidos da micoteca do EcoCentro/EPAMIG em Lavras, MG. O óleo essencial do orégano inibiu o desenvolvimento dos fungos testados em todas as concentrações exceto o fungo A. niger que teve o seu desenvolvimento micelial inibido a partir da concentração de 1000 mg/mL-1,. Os óleos de alecrim, menta, cebola e manjericão tiveram um efeito pronunciado a partir da concentração de 1500 mg/mL-1.


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