Domestic Birds as Source of Cryptococcus deuterogattii (AFLP6/VGII): Potential Risk for Cryptococcosis

Cryptococcosis is an infection caused by encapsulated basidiomycetous yeasts belonging to the Cryptococcus neoformans/Cryptococcus gattii species complexes. It is acquired through inhalation of infectious propagules, often resulting in meningitis and meningoencephalitis. The ecological niche of these agents is a wide variety of trees species, as well as pigeon, parrot and passerine excreta. The objective of this study was to isolate Cryptococcus yeasts from excreta of commercially traded parrots and passerines. The 237 samples were collected between October 2018 and April 2019 and processed using conventional methodologies. Nineteen colonies with a dark brown phenotype, caused by phenol oxidase activity, were isolated, suggesting the presence of pathogenic Cryptococcus yeasts. All isolates tested positive for urease activity. URA5-RFLP fingerprinting identified 14 isolates (68.4%) as C. neoformans (genotype AFLP1/VNI) and 5 (26.3%) as C. deuterogattii (genotype AFLP6/VGII). Multi-locus sequence typing was applied to investigate the relatedness of the C. deuterogattii isolates with those collected globally, showing that those originating from bird-excreta were genetically indistinguishable from some clinical isolates collected during the past two decades.

Novel Nano-Filled Coatings for the Protection of Built Heritage Stone Surfaces

An experimental nano-filled coating, based on a fluorine resin containing SiO2 nano-particles, was applied on calcareous stones, representative of materials used in buildings and monuments of the Mediterranean basin; for comparison purposes, two commercial products were applied on the same substrates. The efficacy of the protective treatments was assessed by analyzing different characteristics of the three experimental/commercial products, i.e., color changes and permeability to water vapor to evaluate the treatments’ harmlessness; capillary water absorption and water stone contact angle to evaluate the protection against water ingress; oleophobicity of the treated surfaces and the behavior under staining by acrylic blue-colored spray paint and felt-tip marker to verify the anti-graffiti action. Finally, the properties of the treated stone surfaces were analyzed also after the application of pancreatin, used to simulate bird excreta (guano). The protective coatings were found to promote graffiti removal, reducing also the detrimental effects due to simulated guano. The experimental nano-filled product, in addition, was able to provide outstanding performance but using smaller amounts of product in comparison to commercial systems.

First Isolation, Antifungal Susceptibility, and Molecular Characterization of Cryptococcus neoformans from the Environment in Croatia

The purpose of this study was to investigate the presence of Cryptococcus neoformans species complex isolates from environmental sources in Croatia and to determine their molecular types and antifungal susceptibility. Swab samples of tree hollows and bird excreta in the soil beneath trees were collected. Samples included 472 (92.73%) samples obtained from tree hollows and 37 (7.27%) samples from bird excreta. Four C. neoformans species complex isolates were recovered from tree hollow swabs along the Mediterranean coast, while there were no isolates recovered from bird excreta or from the continental area. Three isolates were identified as molecular types VNI and one as VNIV. All tested antifungals showed high in vitro activity against the four isolates. This is the first report proving the presence of C. neoformans species complex in the environment of Croatia. The results of the study suggest a major risk of exposure for inhabitants living along the Croatian coast and that both VNI and VNIV molecular types can be expected in clinical cases of cryptococcosis. Susceptibility to antifungals confirmed that no resistance should be expected in patients with cryptococcosis at the present time.

Pigeon Presence on PV Modules ARE Largely Random Events

The presence of pigeons on PV modules can negatively affect the output power of a solar renewable energy system. The body of the pigeon itself (and especially the tail) may cause short periods of shading of individual cells, leading to the formation of hotspots. Bird excreta left by the pigeon may cause longer periods of shading, leading to an extended reduction in output power. Some type of intervention may be required to repel pigeons from PV modules, in order to try and maintain the overall efficiency and sustainability of a system. The purpose of this paper is to evaluate the reduction in output power of a picosolar system in order to determine if a possible pattern, or routine, exists with regard to the presence of pigeons. A 10 W pico-solar system was installed in a semi-arid region of South Africa that is home to the feral pigeon (Columba livia). A pigeon detection technique was developed and applied over a period of 13 months to determine when and for how long these pigeons rest on top of a PV module (these are referred to as events). Although these events are primarily random in nature, results do indicate that feral pigeon presence is lowest on a Wednesday during the week and in the summer periods of January to March during a calendar year. They tend to spend, on average, 118 seconds perched on top a PV module, where their tail and droppings cause the most significant impact in terms of interrupting the direct beam radiation from the sun for an individual cell. It is recommended to use these results in formulating an appropriate intervention that may be used as a scare tactic to repel feral pigeons away from PV modules.

Isolation of Cryptococcus species from the external environments of hospital and academic areas

Introduction: Fungi of the genus Cryptococcus are cosmopolitan and may be agents of opportunistic mycoses in immunocompromised and sometimes immunocompetent individuals. Cryptococcus species are frequently isolated from trees and bird excreta in the environment and infection occurs by inhalation of propagules dispersed in the air. The aim was to investigate Cryptococcus species in bird excreta and tree hollows located in a university hospital area and in an academic area of a university campus. Methodology: A total of 40 samples of bird excreta and 41 samples of tree hollows were collected. The identification of the isolates was done by classical methodology and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: Twenty (62.5%) isolates of Cryptococcus were found in bird excreta and 12 (37.5%) in tree hollows. C. laurentii (currently Papiliotrema laurentii) was the most frequent species in both samples, being found in 5 samples of excreta and in 8 tree hollows. The diversity of species found in excreta (C. laurentii, C. albidus [currently Naganishia albida], C. liquefaciens [currently N. liquefaciens], C. friedmanii [currently N. friedmannii] and others) was higher than in tree hollows (C. laurentii, C. flavescens [currently Papiliotrema flavescens], and other yeasts). Conclusion: Many Cryptococcus species were isolated from excreta and tree hollows, and this fact is important for understanding the environmental epidemiology of those emerging pathogens for public health, as a way to implement surveillance actions and control of cryptococcosis.

Effects of acid pigeon excreta on building conservation

Purpose Birds are implicated in spoiling and decay of buildings, especially through their droppings. Pigeons are considered the main culprits, and several studies have examined the effects and chemistry of accumulations of droppings without evidence to the exact origins of the source of the excreta. The paper aims to discuss these issues. Design/methodology/approach This study reviews and summarises the state of knowledge with regard to the impact of bird excreta on buildings. It experimentally assesses the acidity of fresh pigeon excreta with different diets and examines the development of the acidity of the excreta after voiding. Findings Feral pigeons in urban settings are known to be fed by a range of foods. Urban food scraps-derived diets produce more acidic excreta than more natural diets such as seeds. This is a first study of its kind to examine the impact of a bird’s diet on the pH and thus the resulting (potential) decay of masonry. Research limitations/implications This study showed that from a management’s perspective, pigeons that subsist entirely on human provided foods will be depositing more initially acidic faeces. If faecal accumulation occurs; then, mould and other bacteria quickly alter the chemistry from acidic towards basic, but the damage may already be done. Originality/value This paper is the first study of its kind to examine the effects of fresh pigeon droppings of known origin and age once voided from the intestine. This allows the authors to assess the impact during the first few days.

Screening of antigenemia and isolation of Cryptococcus neoformans and C. gattii from cloaca and crop of birds in the state of Paraná, Brazil

Cryptococcus neoformans and C. gattii are associated with dry bird excreta but rarely recovered from birds' digestive tract. The objective of the present study was (1) to verify the existence of C. neoformans and C. gattii in crop and cloaca of wildlife and captivity birds hypothesizing about a possible primary source of this yeast in the excreta, and (2) to determine the fungi's invasive capability in avian species through latex agglutination. For that purpose, 172 cloacal and 77 crop samples of domestic pigeon, Passerine, and Psittacine birds were collected. None of these samples was positive, suggesting that the yeast is not saprobiotic in the digestive tract of these birds. Only one out of 82 serum samples collected from pigeons and Psittacine birds was positive (title 1:2) showing that Cryptococcus sp. probably has a low invasive capability in birds, and is thus considered only a dry excreta colonizer.