Four decades of epidemiological data reveal trajectories towards malaria elimination in Kheda district (Gujarat), western part of India

BackgroundMalaria is a main public health problem in India and was so particularly in the state of Gujarat in the western part of the country. This study assesses the effects of various interventions on malaria cases using data from the last 33 years (1987–2019).MethodsHere we have analysed 33 years of malaria epidemiological data from a malaria clinic in Kheda district in Gujarat. The data were digitised yearly and monthly, age-wise and gender-wise, and descriptive analysis was performed to assess the effects of several interventions on malaria burden.ResultsDuring 1987–2019, our clinic diagnosed 5466 Plasmodium vivax and 4732 P. falciparum malaria cases. Overall, there was a declining trend in malaria cases except for the years 1991, 1994, 2004 and 2005. The year 2004 especially witnessed an epidemic in Kheda as well as throughout Gujarat. Malaria infections were most common (40%) among the 21–40 years age group. Fever was the most common symptom in all age groups.InterpretationIntroduction of revised drug policy and improved surveillance technique (rapid diagnosis kits) have strengthened the diagnosis and treatment of malaria in the district. Use of pyrethroid in indoor residual insecticide spray has also strengthened vector control. Among the various interventions used, long-lasting insecticide nets and introduction of artemisinin-based combination therapy have played significant roles in controlling malaria cases. A more drastic decline in P. falciparum cases versus P. vivax is evident, but the latter persists in high proportions and therefore new tools for malaria control will be needed for elimination.

Repellant Effect of Urine Insecticides Spray and NPK (15:15:15) Compound Fertilizer Application on the Growth and Yield of watermelon (Citrullu lonatus Thumb)

A field study conducted to investigate the Repellent effects urine insecticide spray and NPK(15:15:15) compound fertilizer on the growth and yield of watermelon (Citrullus Lonatus Thumb) in forestry Research Institute of Nigeria, (FRIN) experimental plot at Agbede, Etsako West Local Government Area of Edo State. Four regimes of Urine insecticide spray and four rate of NPK compound fertilizer application were factorially combined and laid out in a completely randomized block design (CRBD) with three replication. Urine insecticide spray and fertilizer application did not affect the period of flowering and poddring significantly (P> 0.05). NPK compound fertilizer application significantly (P< 0.05) increase the number and length of main vines, pod number and yield while Vines insecticide spray significantly (P< 0.05) increase the length of the main virus and yield. In the rate of application, 200kg/ha NPK was optimum and this recommended for watermelon cultivation. Twice spray regimes of urine insecticide was recommended for watermelon cultivation.

Distribution of Fly Pests in a Farm in Plateau State, Nigeria

An evaluation of fly pests of cattle was conducted in Federal College of Animal Health and Production Technology (FCAH&PT) Farm, Chaha Village, Jos South Local Government Area, Plateau State, Nigeria between August and October, 2020. Samples were collected from the farm weekly for three months. A total of nine hundred and nineteen (919) adult dipterous flies comprising fifteen species in eight genera across the three sub-orders were caught and identified using aerial sweeping hand net, light trapping and aerial insecticide spray. Phlebotomus papaltasi (44.18%) was the most prevalent while Simulium damnosum (0.22%) was the least. Others were Musca domestica (17.73%), Stomoxys calcitrans (11.43%), Culicoides species (10.99%), Musca autumnalis (10.23%), mosquitoes – Anopheles gambiae/Culex quequinfasciatus (4.89%) and Tabanus species (0.33%). The haematophagous flies (72.04%) out-numbered the non-haematophagous flies (27.96%) and the number of females collected/caught 780 (84.87%) were higher than the males 139 (15.13%). The highest collections were made in October (353 representing 38.41%) while September had the lowest (263 /28.62%). The study established the presence of fly pests in the farm and recommends that appropriate control measures should be put in place to forestall any disease outbreak.

Malaria and Insecticide-Treated Net (ITN) Utilization among Orphans and Vulnerable Children in Nigeria

Background: The lack of empirical data on the malaria status of orphans and vulnerable children (OVC), access to long-lasting insecticide-treated nets (LLINs), and knowledge about the disease can hamper the development of effective policies and programs to address their malaria-related needs. Aim: The aim of this study was to evaluate knowledge of malaria transmission and utilization of LLINs among OVC in Ondo metropolis, Nigeria. Materials and Methods: A cross-sectional study was conducted in Ondo City, Ondo State, Nigeria, between January and March 2018. The study was carried out among 66 OVC (age 5–19 years) using questionnaires. Information on socio-demography, LLIN utilization, knowledge and risks of malaria transmission among OVC was sought. Results: The use of LLINs (80.9%) among OVC was significantly higher than the use of insecticide spray (6.1%) and mosquito coils (12.1%) (p <0.0001). Knowledge about malaria transmission was relatively high, and the majority of children (60.6%) believed malaria is mostly transmitted during the dry season. Fewer children (21.2%) had a history of malaria in the last 3 months. Conclusions: The risk of malaria transmission was low among OVC. Continuous accessibility of orphanage homes to LLINs should be sustained to prevent malaria transmission among OVC.

Insecticide spray drift reduction with different adjuvants and spray nozzles

ABSTRACT Insecticide spray drift can lead to reduced control efficiency and loss of product to the environment. Thus, we conducted a study to evaluate the effect of different spray nozzles and the addition of adjuvants in insecticide spray on the resulting droplet spectrum and wind tunnel drift. All experiments were conducted in a completely randomized design with four repetitions using a 5 × 3 factorial scheme. Five spraying solutions were studied; one contained only water and the other four comprised thiamethoxam + lambda-cyhalothrin (no adjuvant, Oro-solve, Wetcit Gold, and Orobor N1), in combination with three spray nozzles (AXI, JFC, and J3D). The droplet spectrum was evaluated through the volumetric median diameter, relative amplitude, and percentage of the droplet volume with diameter ≤ 100 µm. The drifts were evaluated in a wind tunnel at 5, 10, and 15 m. Data were subjected to analysis of variance and means were compared using the Tukey’s test. In addition, a principal component analysis was performed. Application of the insecticide with the adjuvants combined with the different nozzles changed the droplet spectrum and the risk of drift. The AXI nozzle and the J3D associated with the Oro-solve and Wetcit Gold adjuvants resulted in a greater volumetric median diameter of the droplets and drifts were observed at 5 m.

Bacterial Rdl2 dsRNA increased the insecticidal activity of GABAR blockers and allosteric modulators against Plutella xylostella

ABSTRACTBACKGROUNDThe application of RNAi to control pests has attracted the attention of researchers. Our results indicated that knockout of PxRdl2 can decrease resistance to fipronil in Plutella xylostella, providing a suitable target gene for RNAi-based pest control.RESULTSThe differences in the sensitivity of two established homozygous knockout strains of P. xylostella. (Rdl1KO and Rdl2KO) and susceptible P. xylostella to a series of compounds were evaluated. Quinazolines and isoxazolines both showed stronger efficacy in the Rdl2KO strain. Therefore, we proposed a method based on the knockdown of the P. xylostella Rdl2 gene as a tactic to enhance the toxicity of quinazolines and isoxazolines. To reduce costs and protect dsRNA against degradation, we applied a bacterial expression system using the L4440 vector to express PxRdl2 dsRNA in HT115 Escherichia coli. Transformed bacteria (dsRNA-Bac) fed through leaves combined with quinazoline and isoxazolines proved to be more effective in both the susceptible and fipronil-resistant P. xylostella.CONCLUSIONOur results provide a strategy for the development of novel insecticide spray formulations containing dsRNA-Bac, which synergize with insecticide toxins by suppressing PxRdl2, reducing the use of pesticides in the field.

Seven plus initiative: the way forward to stem Dengue

The vector-borne disease has been a public health challenge in the country since long. The menace of dengue, every rainy season only draws attention towards the need for community participation to combat this problem. Dengue has come out to be a social problem which mostly depends upon the environment and habitation of humans. The overwhelming concern over the increasing burden and its dire consequences, a multidisciplinary team was constituted which comprised of local leaders, ASHA, ANM and NGOs, destined to encourage community participation for dengue prevention. A seven-plus initiative was started by this multidisciplinary team which aims to mitigate the larva life cycle of dengue from the breeding site in seven days. This initiative was completely based on active surveillance of breeding sites, tracking fever cases and active implementation of source reduction measures coupled with insecticide spray.