Cervical Spondylopathy and Lumbar Intervertebral Disc Herniation Coexist in Free Radical Metabolism and Focus Separation in the Body

Lumbar disc herniation is a common lumbar disease in clinics, which is related to improper use of lumbar vertebrae and osteoporosis. Surgical removal of nucleus pulposus and maintenance of lumbar structural stability are important for the treatment of lumbar disc herniation. At present, in clinical percutaneous intervertebral foramen endoscopic surgery for lumbar disc herniation, interlaminar and intervertebral foramen approaches can be selected. Different approaches have different degrees of difficulty in the treatment of lumbar disc herniation, and the clinical effects that may be obtained are different. In this study, we observed the influencing factors of plasma nitric oxide (NO) and free radical metabolism in patients with lumbar disc herniation and the correlation between the effects of focus separation. The organic combination of local and total illness differentiation and dialectics, conventional acupuncture, and electrical stimulation was highlighted in this study, which linked local acupoints squeezed by nerve roots with distant acupoints along meridians. The use of authoritative quantitative standards and a multifactor assessment of the disease can accurately represent the disease's severity. The patient's condition changes in each period may be expressed more accurately, thoroughly, and objectively through the rise or reduction of the score, making self-evaluation easier for the patient. Electroacupuncture at point may be one of the most important strategies to minimize free radical damage, based on changes in plasma levels.

PO-118 Effects of field-battle hypoxia supporter on free radical metabolism after exercise at high altitude

Objective To explore the effects of field-battle hypoxia supporter on free radical metabolism after exercise at high altitude． Methods A total of 40 healthy fighters stationed in the 3800m plateau for 10 days were randomly divided into two groups. Each group of 20 men, armed with heavy weapons and hypoxia supporter, hiked 5 km and then climbed a 4,300 m peak, a total distance of 10 km. The control group closed the valve of hypoxia supporter without oxygen inhalation, the experimental group opened the valve and inhaled oxygen. The serum levels of superoxide dismutase(SOD), total antioxidant capacity(T-AOC), reactive oxygen species (ROS) and malondialdehyde (MDA) were measured at rest and after exercise. In addition, The average marching speed of the two groups were compared． Results The T-AOC, ROS and MDA were increased both in the control group and experimental group after exercise, and the SOD increased significanly in experimental group (P<0.01)．Compared with control group, the SOD and T-AOC were increase but the ROS and MDA were decreased significanly after exercise(P<0.01)．The average speed of 10km cross-country march in the control group and the experimental group was (3.0±0.3)km/h and (2.3±0.5)km/h respectively, and there were significant differences (P<0.01). Conclusions The field-battle hypoxia supporter can restrain free radical damage after exercise at high altitude and increase the efficiency of work at high altitude. Acknowledgements: This work was supported by the Guangdong Science and Technology Equipment Mobilization project (Research and application of field-battle hypoxia supporter).

PO-113 Long-term Various Load Training Effects on Free Radical Metabolism in the Brain of Aging Mice

Objective To investigate the effects of long-term various load aerobic exercise on the free radical metabolism of the brain in aging mice through the establishment of different swimming exercise models. Methods sixty 3-month-old male Kunming mice were randomly and equally divided into five groups, which were the control group (group C), 10 minute exercise group (group E1), 30 minute exercise group (group E2), 60 minute exercise group (group E3), and 90 minute exercise group (group E4). Group C had regular feeding during the natural aging time in 8 months without any exercise intervention. All other exercise groups performed different load swimming exercise (from 10 to 90 minutes as mentioned above) during the same 8 month period. All subjects were decapitated after exercise on the last day of the 8th month; and the brain tissues were harvested as samples. The malondialdehyde (MDA) amount was measured by the Thiobarbituric Acid method, and the superoxide dismutase (SOD) activity level was measured with Xanthine Oxidase method. Results group E3 demonstrated significantly higher SOD activity level and lower MDA amount than the other groups. Conclusions Long-term, medium-load aerobic exercise had greatly impacted the metabolism of free radicals in the brain of the mouse, evidenced by increased SOD activity level and decreased MDA production. These effects may indicate that this exercise model may be beneficial in slowing down brain aging by eliminating free radicals and improving brain anti-oxidation ability.

Effect of vitamin D on free radical metabolism in renal tissues of septic rats / Septik ratların böbrek dokularında serbest radikal metabolizmasına vitamin D’nin etkisi

Objective: Sepsis is a common cause of morbidity and mortality in the intensive care unit. Lipopolysaccharide (LPS)-induced excessive immune response is associated with multi-organ damage in sepsis. Excessive immune response causes multi-organ damage by increasing oxidative stress. Studies on the antioxidant effects of vitamin D demonstrated its protective role. In this study we aimed to investigate the effects of vitamin D on free radical metabolism in LPS injected rats.Methods: Twenty four wistar albino rats were separated into control, sepsis, sepsis+vitamin D and vitamin D groups. Sepsis was induced with single injection of LPS Esherichia coli (O111-b4) 16 mg/kg. Vitamin D was given 2 mg/kg 25 (OH) single dose via gavage for 3 days. Renal function tests were analyzed in serum. Tissue catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) activities were analyzed, and rat renal tissues were evaluated histopathologically.Results: SOD and GSH-Px activities were not significantly different between the groups. CAT activities were significantly decreased in all groups compared to control, this suppression was seen in the sepsis+vitamin D group versus sepsis group. GST activities were significantly decreased in sepsis and sepsis+vitamin D group compared to control, but GST activities were significantly elevated vitamin D group compare to sepsis and sepsis+vitamin D group. Blood urea nitrogen (BUN) and creatinine levels were significantly elevated in sepsis and sepsis+vitamin D group. Inflammation, expansion in bowman capsule were detected in sepsis and sepsis+vitamin D groups.Conclusion: Vitamin D treatment does not seem to have protective role against renal toxicity in sepsis.Nutrition with vitamin D in sepsis may have suppressive effect on antioxidant enzymes such as CAT and GST due to reduced substrat level which use hydrogen peroxide (H

Influence of Polysaccharides from Lycium barbarum Fruits on Exercise Tolerance and Free Radical Metabolism in Liver of Mice after Exhaustive Exercise

The purpose of this study was to examine the effects of polysaccharides fromLycium barbarumfruits (LBP) on free radical metabolism of mice after exhaustive swimming exercise. The results showed that LBP supplementation could alleviate fatigue and enhanced the exercise tolerance. Furthermore, LBP supplementation effectively attenuates the exhaustive exercise-induced increased lipid peroxidation and elevates antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase) activities. LBP may have therapeutic role in preventing liver tissue damage during exhaustive exercise.