Antibacterial Activity Test of Various Fraction of Lick leaves (Villebrunea rubescens) against Staphylococcus aureus and Staphylococcus epidermidis Bacterial

Lick leaves (Villebrunea rubescens) are one of the medicinal plants from the Urticaceae family. In Papua, we called it Daun jilat, which is used empirically by the community to treat contusion or bruises in the Serui area, Yapen Island Regency, Papua, Indonesia. Based on the result of the phytochemical screening, V. rubescens contained Alkaloids, Tannins, Saponins, Quinones, and Flavonoids. In previous research on an extract of Lick leaves, it was found that the antibacterial activities within inhibition zone have value of 8.7 mm (125 ?g/mL). This research aims to find out the antibacterial activities of the various fraction of Lick leaves in inhibiting the growth of Staphylococcus epidermidis and Staphylococcus aureus bacterial and to find out the most effective concentration of the various fraction of Lick leaves. The method used in this research was disc diffusion method and the solution used was ethanol-water, ethyl acetate, and N-hexane with the variable concentration used was 100, 250, 500, 750, 1000ppm. The result showed that Lick leaves fraction of ethanol-water, ethyl acetate, and n-hexane had an inhibition zone against the growth of Staphylococcus epidermidis bacteria, while the n-hexane and ethyl acetate had an inhibition zone against the growth of Staphylococcus aureus bacteria. However, for the ethyl-acetate, the inhibition zone was categorized as weak. The concentration of Lick leaves which effectively inhibited the growth of bacteria was ethanol-water fraction with an inhibition zone of 7.78 mm against Staphylococcus epidermidis bacteria and ethanol-water fraction with an inhibition zone of 3.89 mm against Staphylococcus aureus bacteria.

Download Full-text

Analysis of The Phytochemical Characteristics of Jasmine Flower Against Escherichia coli and Staphylococcus aureus

Microbiology Indonesia ◽

10.5454/mi.15.3.4 ◽

2021 ◽

Vol 15 (3) ◽

pp. 4

Author(s):

Nathasa Sihite

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Ethyl Acetate ◽

Inhibitory Effect ◽

Diffusion Method ◽

Agricultural Product ◽

Disc Diffusion Method ◽

Water Solvent ◽

Jasminum Sambac ◽

And Staphylococcus Aureus

Indonesia is a famous country had the diversity of plants, especially agricultural product and herbs, they had a potential of antimicrobial as a like Jasmine Flower (Jasminum sambac Ait). This study Indonesia is a famous country had the diversity of plants, especially agricultural product and herbs, they had a potential of antimicrobial as a like Jasmine Flower (Jasminum sambac Ait). This study aimed to determine the phytochemical and antimicrobial activity of jasmine flower. Extraction was done by maceration, such as using water solvent, methanol, ethyl acetate, and hexane as the solvent. The sample bacteria used were Escherichia coli and Staphylococcus aureus. This study used disc diffusion method, with a complete 2-factor random design and 4 replications. Processing of the data is using ANOVA. The results showed that the jasmine extracts with a concentration of 25%, 50%, 75% and 100% had an effect to inhibit of the growth of the bacteria. Jasmine extract with ethyl acetate solvent was the most effective to inhibit the growth of bacteria. It was known that jasmine flower extract is one of the good natural antimicrobials gave an inhibitory effect to the growth of Escherichia coli and Staphylococcus aureus bacteria. Keywords: jasmine, Escherichia coli, Sthaphylococcus aureus, antimicrobials

Download Full-text

AKTIVITAS ANTIBAKTERI DAN ANTI-UV BEBERAPA ASCIDIAN DARI PERAIRAN PANGALISANG BUNAKEN

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.7.3.2019.26019 ◽

2019 ◽

Vol 7 (3) ◽

pp. 271

Author(s):

Yuliana Macpal ◽

Veibe Warouw ◽

Deiske A Sumilat ◽

James J.H. Paulus ◽

Natalie D.C. Rumampuk ◽

...

Keyword(s):

Antibacterial Activity ◽

Marine Invertebrates ◽

Antibacterial Activities ◽

Diffusion Method ◽

Methanol Fraction ◽

Water Fraction ◽

E Coli ◽

Activity Test ◽

Result Show ◽

And Staphylococcus Aureus

Ascidians are sesile marine invertebrates that have bioactive compounds such as antibacterial and anti-UV. The purpose of this study is to determine the antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and to test the anti-UV activity of the ascidian water fraction. Antibacterial activity test was carried out using the diffusion method (disc diffusion Kirby & Bauer) and the water fraction of ascidian that showing antibacterial activity tested in a UV spectrophotometer to see the anti-UV activity. Results of the study were obtained 4 types of ascidians extracted from partitioned into water fraction, n-hexane fraction, methanol fraction. All three fractions were tested for antibacterial activity and the result showed that there were antibacterial activities of ascidian extract Clavelina sp. against both test bacteria with inhibition of S. aureus and E. coli with strong categories. Ascidian Phlebobranch sp. showed the presence of antibacterial activity with inhibition of S. aureus and E. coli with very strong categories (16,6 mm). extract Eudistoma sp. showed the presence of antibacterial activity with inhibition S. aureus and E. coli in the medium category (9 mm). The water fraction found active in antibacterial testing is then tested using a UV spectrophotometer for anti UV testing, the result show that water fraction of the four ascidians can absorb UV-B (290-320nm) and UV-A ( 320-400nm).Keywords : Ascidian, Antibacterial, Partition, Anti- UV.

Download Full-text

POTENSI EKSTRAK TUMBUHAN ANDALIMAN (Zanthoxylum acanthopodium DC.) SEBAGAI ANTIBAKTERI Staphylococcus aureus

Alotrop ◽

10.33369/atp.v4i2.13864 ◽

2019 ◽

Vol 2 (2) ◽

pp. 133-139

Author(s):

Oma Sepriani ◽

Nurhamidah Nirhamidah ◽

Dewi Handayani

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Secondary Metabolite ◽

Inhibition Zone ◽

Stem Bark ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Activity Test ◽

North Sumatra

The aims of this study is to determine the secondary metabolite compounds in Roots, stem bark, leaves and fruit of Andaliman (Zanthoxylum acanthopodium DC), and determine the most potent part of Andaliman plant extract in inhibiting the growth of Staphylococcus aureus bacteria. Andaliman plant taken in Simarpingan, Sipaholon village sub district of North Sumatra. Part of the Andaliman plantar are cleaned, cut into small pieces and then dried and mashed, samples that have been finely phytochemically tested and extracted. Testing of secondary metabolite compound by doing phytochemical screening of roots, stem bark, leaves and fruit of Andaliman, alkaloid test using meyers reagent, terpenoid test and steroid using TLC plate with eluent n-hexana : ethyl acetate with ratio 6:4, flavonoid test using soluton HCl and Mg band, saponin test using soap test, tannin test using ferro (III) chloride solution. Test of antibacterial activity using disc diffusion method. Phytochemical results showed that all parts of the plant andaliman contain secondary metabolite compounds namely alkaloids, flavonoids, terpenoids, tannins and also saponins. The result of antibacterial activity test of leaf extract of leaf and leaf which has the most potential as antibacterial of S.aureus is bark. Because it able to inhibit S.aureus growth with 11 mm inhibition zone at concentration 5x104 ppm, which is included in the strong category. In addition to skin of andaliman leaf stem is also potential as an antibacterial with a 10 mm inhibition zone at concentration 5x104 ppm, which is included in the medium category.

Download Full-text

AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Biomedika ◽

10.23917/biomedika.v4i2.252 ◽

2012 ◽

Vol 4 (2) ◽

Author(s):

Dr. Muhtadi , MSi. ◽

Ria Ambarwati ◽

Ratna Yuliani

Keyword(s):

Antibacterial Activity ◽

Klebsiella Pneumoniae ◽

Ethyl Acetate ◽

Staphylococcus Epidermidis ◽

Antibacterial Properties ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Water Fraction

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

Download Full-text

Uji Aktivitas Antibakteri Ekstrak Metanol dan Fraksi Daun Kenikir (Cosmos caudatus Kunth) terhadap Salmonella typhi

Pustaka Kesehatan ◽

10.19184/pk.v8i3.13008 ◽

2020 ◽

Vol 8 (3) ◽

pp. 177

Author(s):

Achmad Syarifudin Noor ◽

Bawon Triatmoko ◽

Nuri Nuri

Keyword(s):

Antibacterial Activity ◽

Typhoid Fever ◽

Ethyl Acetate ◽

Methanol Extract ◽

Inhibition Zone ◽

Salmonella Typhi ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Water Fraction ◽

Zone Diameter

The leaves of kenikir are known to have many benefits, one of which can be used as medicine. Kenikir leaves are reported to have antibacterial activity against the bacteria Salmonella typhi (S.typhi). S. typhi is a bacterium that causes typhoid fever. Typhoid fever is still an endemic disease in Asian countries, especially Indonesia. Typhoid fever became the third largest disease in Indonesia in 2010. The study related to the antibacterial activity of kenikir leaf fraction to S. typhi has never been reported. Therefore, it is necessary to conduct research related to the antibacterial activity of extracts and leaf fractions of kenikir to compare their potential against S. typhi. The study was conducted using the disk diffusion method using 5 series of concentrations namely 5%, 10%, 15%, 20%, and 30%. Tests were carried out on methanol extract, hexane, ethyl acetate, and methanol-water fraction. The results showed that highest inhibition zone diameter is 7,75 mm which is reached by methanol extract. The order of the highest inhibiting zone diameter are extract, hexane, ethyl acetate, and methanol-water fraction.

Download Full-text

Synthesis and Antibacterial Activities of N-Phenylpyrazolines from Veratraldehyde

Materials Science Forum ◽

10.4028/www.scientific.net/msf.901.124 ◽

2017 ◽

Vol 901 ◽

pp. 124-132

Author(s):

Artania Adnin Tri Suma ◽

Tutik Dwi Wahyuningsih ◽

Deni Pranowo

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Bacillus Cereus ◽

Shigella Flexneri ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Diffusion Method ◽

Agar Well Diffusion Method

Some novel N-phenylpyrazolines were synthesized and investigated for their antibacterial activitiy. Chalcones 2-4 which were prepared from acetophenone and veratraldehyde derivatives were reacted with phenylhydrazine to give N-phenylpyrazolines 5-7. All of the synthesized compounds were characterized using FTIR, GC-MS, and NMR spectrometers. Further, antibacterial activity of N-phenylpyrazolines were evaluated by agar well-diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Shigella flexneri. The highest activity (highest inhibition zone) of compound 5 was 2.6 mm (at 1000 ppm) against B. subtillis, compound 6 was 7.25 mm (at 1000 ppm) against S. aureus, and compound 7 was 6.75 mm (at 500 ppm) against S. aureus. The results indicated that compound 6 and 7 exhibited promising antibacterial activity.

Download Full-text

Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal MIPA ◽

10.35799/jm.3.2.2014.5989 ◽

2014 ◽

Vol 3 (2) ◽

pp. 129

Author(s):

Megawati S. Saroinsong ◽

Febby E. F. Kandou ◽

Adelfia Papu ◽

Marina F. O. Singkoh

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methanol Extract ◽

Inhibition Zone ◽

E Coli ◽

Activity Test ◽

And Staphylococcus Aureus ◽

Paper Disc ◽

Coli Growth

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.

Download Full-text

UJI AKTIVITAS ANTIBAKTERI DARI ISOLAT CENDAWAN ENDOFIT ASAL TUMBUHAN SENDUDUK (Melastoma malabathricum L.) TERHADAP Escherichia coli DAN Staphylococcus aureus

Photon: Jurnal Sain dan Kesehatan ◽

10.37859/jp.v8i2.741 ◽

2018 ◽

Vol 8 (2) ◽

pp. 7-12

Author(s):

Israwati Harahap ◽

Vivin Paddillah Rahmi ◽

Nofripa Herlina

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Endophytic Fungi ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Zone Of Inhibition ◽

Melastoma Malabathricum ◽

Symbiotic Microorganism ◽

And Staphylococcus Aureus ◽

Test Result

Endophytic fungi is a symbiotic microorganism which live inside plant tissues and not harm to their host. Several genera from endophytic fungi known to produce secondary metabolite compounds like antibiotics, anticancer, antifungal, antiviral and antimalarial. This study aimed to investigate antibacterial activity of endophytic fungi from senduduk (Melastoma malabathricum L.) against Escherichia coli and Staphylococcus aureus. Based on the test result, 11 isolates endophytic fungi from senduduk were known to have antibacterial activities. Isolate code 23 have ability to inhibit the growth of E.coli with diameters zone of inhibition is 22 mm and eight isolate endophytic fungi have ability to inhibit the growth of S.aureus (showed by inhibition zone).

Download Full-text

AKTIVITAS ANTIMIKROBA EKSTRAK DAUN JAMBU BIJI DARI BEBERAPA KULTIVAR TERHADAP Staphylococcus aureus ATCC 25923 DENGAN HOLE-PLATE DIFFUSION METHOD

Journal of Biological Researches ◽

10.23869/bphjbr.9.1.200310 ◽

2003 ◽

Vol 9 (1) ◽

pp. 49-51

Author(s):

Farida Lanawati Darsono ◽

Stephanie Devi Artemisia

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Traditional Medicine ◽

Tween 80 ◽

Antibacterial Activities ◽

Psidium Guajava ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Gram Positive Bacteria ◽

Activity Test

A study has been performed on the antimicrobial activities of jambu biji (Psidium guajava Linn) leaves from several cultivars (red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of jambu biji are frequently used in traditional medicine as a remedy against diarrhoea. The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of jambu biji for each cultivar were obtained by reflux with ethanol 96 percent. The concentrations of the extracts applied to the holes were 10 percent, 20 percent, and 30 percent w/v, the extracts were reconstituted with tween 80 and ethanol 96 percent. Based on the result of the study, it can be concluded that the extract of jambu biji from each cultivar with the concentration of 10 percent, 20 percent, and 30 percent w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anava Factorial 3x3 and furthery tested for significancy (a = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923.

Download Full-text

Elucidating Anti-Acne Properties of a Novel Herbal Moisturizer

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2020/v32i3530976 ◽

2020 ◽

pp. 22-27

Author(s):

Abdul Hafeez ◽

Shmmon Ahmad ◽

Anmar Al- Taie

Keyword(s):

Staphylococcus Aureus ◽

Staphylococcus Epidermidis ◽

Curcuma Longa ◽

Acne Vulgaris ◽

Moisture Loss ◽

Diffusion Method ◽

Ocimum Sanctum ◽

Physical Parameters ◽

Disc Diffusion Method ◽

Dermatological Condition

Acne vulgaris is most common dermatological condition that affects more than 70% of teenagers and more than 10% of adults. The moisture content of human skin makes it appear young and primary function of moisturizer is to avoid the dehydration by preventing the moisture loss. Natural therapies are more suitable and they are more reliable with less or no side effects. The current research focused on the use of herbs for treating acne vulgaris as a moisturizer. This research focused on the use of Azadirachta indica, Curcuma longa, and Ocimum sanctum to prepare the formulation, Azadiracta indica, Ocimum sanctum and Curcuma longa possessed the significant capability for inhibiting acne. The antimicrobial activity was evaluated against the Staphylococcus aureus, Staphylococcus epidermidis and Propionibacterium by disc diffusion method. Prepared formulations were tested for physical parameters such as pH, spreadability, viscosity and color. Formulation studies was found to optimum for all parameters. The study showed the anti-acne moisturizer was significantly effective in controlling the bacteria which cause acne, i.e. Staphylococcus aureus, Staphylococcus epidermidis and Propionibacterium. From this research, can be inferred that formulated herbal anti-acne moisturizer was related with a substantial decrease in the growth of microbes that causes acne.

Download Full-text