An experimental investigation was conducted to disclose the outcomes of oxygenate mixture as additives in Jatropha biodiesel on the performance, combustion, and emission characteristics of a direct injection compression ignition engine. The experiments were conducted in an instrumented single-cylinder, air-cooled, four-stroke, direct-injection diesel engine, equipped with data acquisition system, AC alternator, and an electric loading device. Four oxygenate additives, namely, Ethylene Glycol (C2H6O2), Di methyl Carbonate (C3H6O3), 2-Butoxyethanol (C6H14O2), & Propylene Glycol (C3H8O2) were selected and nine different combinational oxygenate test fuels were prepared attaining ratios of 1, 2, and 4% volume of oxygenates with biodiesel. A significant reduction of emissions such as CO by 60%, Unburned HC by 11%, and smoke emissions by 27% were observed. Substantial improvement in brake thermal efficiency by 6% was observed, while NO emission increased marginally by 4%.