scholarly journals No Increased DNA Damage Observed in the Brain, Liver, and Lung of Fetal Mice Treated With Ethylnitrosourea and Exposed to UMTS Radiofrequency Electromagnetic Fields

2020 ◽  
Vol 41 (8) ◽  
pp. 611-616
Author(s):  
Alexander Lerchl ◽  
Melanie Klose ◽  
Karen Drees
Keyword(s):  
Dna Damage ◽  
Electromagnetic Fields ◽  
Radiofrequency Electromagnetic Fields ◽  
Fetal Mice ◽  
The Brain

scholarly journals Effects of 1950 MHz radiofrequency electromagnetic fields on Aβ processing in human neuroblastoma and mouse hippocampal neuronal cells

2017 ◽  
Vol 59 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Jeongyeon Park ◽  
Jong Hwa Kwon ◽  
Nam Kim ◽  
Kiwon Song
Keyword(s):  
Protein Expression ◽  
Electromagnetic Fields ◽  
Mrna Level ◽  
Neuronal Cells ◽  
Neural Cells ◽  
Continuous Exposure ◽  
Mrna Levels ◽  
Human Neuroblastoma ◽  
Radiofrequency Electromagnetic Fields ◽  
The Brain

Abstract Alzheimer’s disease (AD) is a neurodegenerative disease leading to progressive loss of memory and other cognitive functions. One of the well-known pathological markers of AD is the accumulation of amyloid-beta protein (Aβ), and its plaques, in the brain. Recent studies using Tg-5XFAD mice as a model of AD have reported that exposure to radiofrequency electromagnetic fields (RF-EMF) from cellular phones reduced Aβ plaques in the brain and showed beneficial effects on AD. In this study, we examined whether exposure to 1950 MHz RF-EMF affects Aβ processing in neural cells. We exposed HT22 mouse hippocampal neuronal cells and SH-SY5Y human neuroblastoma cells to RF-EMF (SAR 6 W/kg) for 2 h per day for 3 days, and analyzed the mRNA and protein expression of the key genes related to Aβ processing. When exposed to RF-EMF, mRNA levels of APP, BACE1, ADAM10 and PSEN1 were decreased in HT22, but the mRNA level of APP was not changed in SH-SY5Y cells. The protein expression of APP and BACE1, as well as the secreted Aβ peptide, was not significantly different between RF-EMF–exposed 7w-PSML, HT22 and SH-SY5Y cells and the unexposed controls. These observations suggest that RF-EMF exposure may not have a significant physiological effect on Aβ processing of neural cells in the short term. However, considering that we only exposed HT22 and SH-SY5Y cells to RF-EMF for 2 h per day for 3 days, we cannot exclude the possibility that 1950 MHz RF-EMF induces physiological change in Aβ processing with long-term and continuous exposure.

scholarly journals The Protective Effect of Autophagy on DNA Damage in Mouse Spermatocyte-Derived Cells Exposed to 1800 MHz Radiofrequency Electromagnetic Fields

2018 ◽  
Vol 48 (1) ◽  
pp. 29-41 ◽  
Author(s):  
Renyan Li ◽  
Mingfu Ma ◽  
Lianbing Li ◽  
Letian Zhao ◽  
Tianfeng Zhang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Western Blot ◽  
Signaling Pathway ◽  
Electromagnetic Fields ◽  
Ros Generation ◽  
Molecular Signaling ◽  
Radiofrequency Electromagnetic Fields ◽  
Transmission Electron ◽  
Amp Activated Protein Kinase

Background/Aims: The effects of exposure to radiofrequency electromagnetic fields (RF-EMFs) on the male reproductive system have raised public concern and studies have shown that exposure to RF-EMFs can induce DNA damage and autophagy. However, there are no related reports on the role of autophagy in DNA damage in spermatocytes, especially after exposure to RF-EMFs. The aim of the present study was to determine the mechanism and role of autophagy induced by RF-EMFs in spermatozoa cells. Methods: Mouse spermatocyte-derived cells (GC-2) were exposed to RF-EMFs 4 W/kg for 24 h. The level of reactive oxygen species (ROS) was determined by ROS assay kit. Comet assay was utilized to detect DNA damage. Autophagy was detected by three indicators: LC3II/LC3I, autophagic vacuoles, and GFP-LC3 dots, which were measured by western blot, transmission electron microscopy, and transfection with GFP-LC3, respectively. The expression of the molecular signaling pathway AMP-activated protein kinase (AMPK)/mTOR was determined by western blot. Results: The results showed that RF-EMFs induced autophagy and DNA damage in GC-2 cells via ROS generation, and the autophagy signaling pathway AMPK/mTOR was activated by ROS generation. Furthermore, following inhibition of autophagy by knockdown of AMPKα, increased DNA damage was observed in GC-2 cells following RF-EMFs exposure, and overexpression of AMPKα promoted autophagy and attenuated DNA damage. Conclusions: These findings demonstrated that the autophagy which was induced by RF-EMFs via the AMPK/mTOR signaling pathway could prevent DNA damage in spermatozoa cells.

scholarly journals Cell Type-Dependent Induction of DNA Damage by 1800 MHz Radiofrequency Electromagnetic Fields Does Not Result in Significant Cellular Dysfunctions

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54906 ◽  
Author(s):  
Shanshan Xu ◽  
Guangdi Chen ◽  
Chunjing Chen ◽  
Chuan Sun ◽  
Danying Zhang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Electromagnetic Fields ◽  
Cell Type ◽  
Radiofrequency Electromagnetic Fields

Developing GIS portal to ensure sanitary and epidemiological surveillance of transferring radio engineering objects

2020 ◽  
Vol 99 (4) ◽  
pp. 344-350
Author(s):  
Evgeny V. Zibarev ◽  
A. S. Afanasev ◽  
O. V. Slusareva ◽  
T. I. Muragimov ◽  
V. A. Stepanets ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Analytical Data ◽  
Base Stations ◽  
Epidemiological Surveillance ◽  
Graphical Form ◽  
Residential Areas ◽  
Information Portal ◽  
Radiofrequency Electromagnetic Fields ◽  
Online Mode ◽  
Automated Processes

In recent years, in the Russian Federation there has been an increase in the levels of radiofrequency electromagnetic fields in residential areas, including due to an increase in the number of base stations (BS). The purpose of sanitary and epidemiological surveillance at the stages of placement and commissioning of base stations (BS) is to prevent their adverse effects on public health. The increase in the number of base stations, together with the advent of new electronic equipment and antennas, provide opportunities for improving the processes of their accounting at the stage of placement and monitoring of the levels of radiofrequency electromagnetic fields at the operation stage. This automation tool can be a geo-information portal for providing sanitary and epidemiological surveillance of cellular base stations. The prototype of the geo-information portal allows both calculating the size of sanitary protection zones (SPZ) and building restriction zones (RZ) from the BS in online mode, displaying the results of calculations in graphical form and issuing sanitary and epidemiological conclusions for the placement and operation of base stations. The geo-information portal has the ability to synchronize with the data of the radio frequency center. Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing will be able to receive up-to-date analytical data. There will be completely automated processes of collecting, processing and storing information on BS.

DNA Damage and Repair in the Brain After Cerebral Ischemia

2001 ◽  
Vol 1 (6) ◽  
pp. 483-495 ◽  
Author(s):  
Bentham Science Publisher Philip K. Liu
Keyword(s):  
Dna Damage ◽  
Cerebral Ischemia ◽  
Dna Damage And Repair ◽  
The Brain

scholarly journals Personal Exposure Assessment to Wi-Fi Radiofrequency Electromagnetic Fields in Mexican Microenvironments

2021 ◽  
Vol 18 (4) ◽  
pp. 1857
Author(s):  
Raquel Ramirez-Vazquez ◽  
Jesus Gonzalez-Rubio ◽  
Isabel Escobar ◽  
Carmen del Pilar Suarez Rodriguez ◽  
Enrique Arribas
Keyword(s):  
Risk Perception ◽  
Electromagnetic Fields ◽  
Personal Exposure ◽  
Wireless Internet ◽  
European Cities ◽  
Radiofrequency Electromagnetic Fields ◽  
Time Period ◽  
The Mean ◽  
Personal Exposure Assessment ◽  
Study Participants

In recent years, personal exposure to Radiofrequency Electromagnetic Fields (RF-EMF) has substantially increased, and most studies about RF-EMF with volunteers have been developed in Europe. To the best of our knowledge, this is the first study carried out in Mexico with personal exposimeters. The main objective was to measure personal exposure to RF-EMF from Wireless Fidelity or wireless Internet connection (Wi-Fi) frequency bands in Tamazunchale, San Luis Potosi, Mexico, to compare results with maximum levels permitted by international recommendations and to find if there are differences in the microenvironments subject to measurements. The study was conducted with 63 volunteers in different microenvironments: home, workplace, outside, schools, travel, and shopping. The mean minimum values registered were 146.5 μW/m2 in travel from the Wi-Fi 2G band and 116.8 μW/m2 at home from the Wi-Fi 5G band, and the maximum values registered were 499.7 μW/m2 and 264.9 μW/m2 at the workplace for the Wi-Fi 2G band and the Wi-Fi 5G band, respectively. In addition, by time period and type of day, minimum values were registered at nighttime, these values being 129.4 μW/m2 and 93.9 μW/m2, and maximum values were registered in the daytime, these values being 303.1 μW/m2 and 168.3 μW/m2 for the Wi-Fi 2G and Wi-Fi 5G bands, respectively. In no case, values exceeded limits established by the International Commission on Non-Ionizing Radiation Protection (ICNIRP). Of the study participants (n = 63), a subgroup (n = 35) answered a survey on risk perception. According to these results, the Tamazunchale (Mexico) population is worried about this situation in comparison with several European cities; however, the risk perception changes when they are informed about the results for the study.

scholarly journals Genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Isela Álvarez-González ◽  
Scarlett Camacho-Cantera ◽  
Patricia Gómez-González ◽  
Michael J. Rendón Barrón ◽  
José A. Morales-González ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Dna Damage ◽  
Mouse Brain ◽  
Control Level ◽  
Dna Oxidation ◽  
Methyl Methanesulfonate ◽  
Control Group ◽  
Oxidative Potential ◽  
The Brain ◽  
Oxidative Effect

AbstractWe evaluated the duloxetine DNA damaging capacity utilizing the comet assay applied to mouse brain and liver cells, as well as its DNA, lipid, protein, and nitric oxide oxidative potential in the same cells. A kinetic time/dose strategy showed the effect of 2, 20, and 200 mg/kg of the drug administered intraperitoneally once in comparison with a control and a methyl methanesulfonate group. Each parameter was evaluated at 3, 9, 15, and 21 h postadministration in five mice per group, except for the DNA oxidation that was examined only at 9 h postadministration. Results showed a significant DNA damage mainly at 9 h postexposure in both organs. In the brain, with 20 and 200 mg/kg we found 50 and 80% increase over the control group (p ≤ 0.05), in the liver, the increase of 2, 20, and 200 mg/kg of duloxetine was 50, 80, and 135% in comparison with the control level (p ≤ 0.05). DNA, lipid, protein and nitric oxide oxidation increase was also observed in both organs. Our data established the DNA damaging capacity of duloxetine even with a dose from the therapeutic range (2 mg/kg), and suggest that this effect can be related with its oxidative potential.

ROS release and Hsp70 expression after exposure to 1,800 MHz radiofrequency electromagnetic fields in primary human monocytes and lymphocytes

2006 ◽  
Vol 45 (1) ◽  
pp. 55-62 ◽  
Author(s):  
M. Lantow ◽  
M. Lupke ◽  
J. Frahm ◽  
M. O. Mattsson ◽  
N. Kuster ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Hsp70 Expression ◽  
Human Monocytes ◽  
Radiofrequency Electromagnetic Fields ◽  
Monocytes And Lymphocytes

scholarly journals Modulation of Crystallinity through Radiofrequency Electromagnetic Fields in PLLA/Magnetic Nanoparticles Composites: A Proof of Concept

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4300
Author(s):  
Marta Multigner ◽  
Irene Morales ◽  
Marta Muñoz ◽  
Victoria Bonache ◽  
Fernando Giacomone ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Magnetic Nanoparticles ◽  
Electromagnetic Fields ◽  
Biomedical Applications ◽  
Infrared Camera ◽  
Proof Of Concept ◽  
Scanning Calorimetry ◽  
Radiofrequency Electromagnetic Fields ◽  
Degradation Properties ◽  
Heat Released

To modulate the properties of degradable implants from outside of the human body represents a major challenge in the field of biomaterials. Polylactic acid is one of the most used polymers in biomedical applications, but it tends to lose its mechanical properties too quickly during degradation. In the present study, a way to reinforce poly-L lactic acid (PLLA) with magnetic nanoparticles (MNPs) that have the capacity to heat under radiofrequency electromagnetic fields (EMF) is proposed. As mechanical and degradation properties are related to the crystallinity of PLLA, the aim of the work was to explore the possibility of modifying the structure of the polymer through the heating of the reinforcing MNPs by EMF within the biological limit range f·H < 5·× 109 Am−1·s−1. Composites were prepared by dispersing MNPs under sonication in a solution of PLLA. The heat released by the MNPs was monitored by an infrared camera and changes in the polymer were analyzed with differential scanning calorimetry and nanoindentation techniques. The crystallinity, hardness, and elastic modulus of nanocomposites increase with EMF treatment.

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