The effect of surface charge property on Escherichia coli initial adhesion and subsequent biofilm formation

2012 ◽  
Vol 109 (7) ◽  
pp. 1745-1754 ◽  
Author(s):  
Akihiko Terada ◽  
Keisuke Okuyama ◽  
Megumi Nishikawa ◽  
Satoshi Tsuneda ◽  
Masaaki Hosomi
Keyword(s):  
Escherichia Coli ◽  
Surface Charge ◽  
Biofilm Formation ◽  
Initial Adhesion ◽  
Charge Property ◽  
Surface Charge Property ◽  
Effect Of Surface

scholarly journals The Transcriptional Antiterminator RfaH Represses Biofilm Formation in Escherichia coli

2006 ◽  
Vol 188 (4) ◽  
pp. 1316-1331 ◽  
Author(s):  
Christophe Beloin ◽  
Kai Michaelis ◽  
Karin Lindner ◽  
Paolo Landini ◽  
Jörg Hacker ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Wild Type Strain ◽  
Wild Type ◽  
Strain Mg1655 ◽  
Upec Strain ◽  
E Coli ◽  
Initial Adhesion ◽  
State Production ◽  
K 12

ABSTRACT We investigated the influence of regulatory and pathogenicity island-associated factors (Hha, RpoS, LuxS, EvgA, RfaH, and tRNA5 Leu) on biofilm formation by uropathogenic Escherichia coli (UPEC) strain 536. Only inactivation of rfaH, which encodes a transcriptional antiterminator, resulted in increased initial adhesion and biofilm formation by E. coli 536. rfaH inactivation in nonpathogenic E. coli K-12 isolate MG1655 resulted in the same phenotype. Transcriptome analysis of wild-type strain 536 and an rfaH mutant of this strain revealed that deletion of rfaH correlated with increased expression of flu orthologs. flu encodes antigen 43 (Ag43), which mediates autoaggregation and biofilm formation. We confirmed that deletion of rfaH leads to increased levels of flu and flu-like transcripts in E. coli K-12 and UPEC. Supporting the hypothesis that RfaH represses biofilm formation through reduction of the Ag43 level, the increased-biofilm phenotype of E. coli MG1655rfaH was reversed upon inactivation of flu. Deletion of the two flu orthologs, however, did not modify the behavior of mutant 536rfaH. Our results demonstrate that the strong initial adhesion and biofilm formation capacities of strain MG1655rfaH are mediated by both increased steady-state production of Ag43 and likely increased Ag43 presentation due to null rfaH-dependent lipopolysaccharide depletion. Although the roles of rfaH in the biofilm phenotype are different in UPEC strain 536 and K-12 strain MG1655, this study shows that RfaH, in addition to affecting the expression of bacterial virulence factors, also negatively controls expression and surface presentation of Ag43 and possibly another Ag43-independent factor(s) that mediates cell-cell interactions and biofilm formation.

scholarly journals Effect of Surface Charge and Hydrophobicity Modulation on the Antibacterial and Antibiofilm Potential of Magnetic Iron Nanoparticles

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Rania Ibrahim Shebl ◽  
Faten Farouk ◽  
Hassan Mohamed El-Said Azzazy
Keyword(s):  
Surface Charge ◽  
Biofilm Formation ◽  
E Coli ◽  
Inhibitory Potential ◽  
Average Size ◽  
Positively Charged ◽  
Significant Concentration ◽  
Effect Of Surface ◽  
Antibacterial Potential ◽  
Trimethoxy Silane

Unmodified magnetic nanoparticles (MNPs) lack antibacterial potential. We investigated MNPs surface modifications that can impart antibacterial activity. Six MNPs species were prepared and characterized. Their antibacterial and antibiofilm potentials, surface affinity, and cytotoxicity were evaluated. Prepared MNPs were functionalized with citric acid, amine group, amino-propyl trimethoxy silane (APTMS), arginine, or oleic acid (OA) to give hydrophilic and hydrophobic MNPs with surface charge ranging from −30 to +30 mV. Prepared MNPs were spherical in shape with an average size of 6–15 nm. Hydrophobic (OA-MNPs) and positively charged MNPs (APTMS-MNPs) had significant concentration dependent antibacterial effect. OA-MNPs showed higher inhibitory potential againstS. aureusandE. coli(80%) than APTMS-MNPs (70%). Both particles exhibited surface affinity toS. aureusandE. coli.Different concentrations of OA-MNPs decreasedS. aureusandE. colibiofilm formation by 50–90%, while APTMS-MNPs reduced it by 30–90%, respectively. Up to 90% of preformed biofilms ofS. aureusandE. coliwere destroyed by OA-MNPs and APTMS-MNPs. In conclusion, surface positivity and hydrophobicity enhance antibacterial and antibiofilm properties of MNPs.

Electrophoresis of pH-regulated nanoparticles: impact of the Stern layer

2016 ◽  
Vol 18 (15) ◽  
pp. 9927-9934 ◽  
Author(s):  
Lanju Mei ◽  
Tzung-Han Chou ◽  
Yu-Shen Cheng ◽  
Ming-Jiang Huang ◽  
Li-Hsien Yeh ◽  
...  
Keyword(s):  
Surface Charge ◽  
Silica Nanoparticles ◽  
Stern Layer ◽  
Layer Effect ◽  
Charge Property ◽  
Surface Charge Property

The Stern layer effect on the surface charge property and electrophoretic motion of pH-regulated silica nanoparticles is investigated theoretically.

scholarly journals Biofilm Formation Potential of Heat-Resistant Escherichia coli Dairy Isolates and the Complete Genome of Multidrug-Resistant, Heat-Resistant Strain FAM21845

2017 ◽  
Vol 83 (15) ◽  
Author(s):  
Roger Marti ◽  
Michael Schmid ◽  
Sandra Kulli ◽  
Kerstin Schneeberger ◽  
Javorka Naskova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stainless Steel ◽  
Biofilm Formation ◽  
Food Production ◽  
Osmotic Shock ◽  
Multidrug Resistant ◽  
Formation Potential ◽  
Conjugative Plasmids ◽  
Heat Resistant ◽  
Initial Adhesion

ABSTRACT We tested the biofilm formation potential of 30 heat-resistant and 6 heat-sensitive Escherichia coli dairy isolates. Production of curli and cellulose, static biofilm formation on polystyrene (PS) and stainless steel surfaces, biofilm formation under dynamic conditions (Bioflux), and initial adhesion rates (IAR) were evaluated. Biofilm formation varied greatly between strains, media, and assays. Our results highlight the importance of the experimental setup in determining biofilm formation under conditions of interest, as correlation between different assays was often not a given. The heat-resistant, multidrug-resistant (MDR) strain FAM21845 showed the strongest biofilm formation on PS and the highest IAR and was the only strain that formed significant biofilms on stainless steel under conditions relevant to the dairy industry, and it was therefore fully sequenced. Its chromosome is 4.9 Mb long, and it harbors a total of five plasmids (147.2, 54.2, 5.8, 2.5, and 1.9 kb). The strain carries a broad range of genes relevant to antimicrobial resistance and biofilm formation, including some on its two large conjugative plasmids, as demonstrated in plate mating assays. IMPORTANCE In biofilms, cells are embedded in an extracellular matrix that protects them from stresses, such as UV radiation, osmotic shock, desiccation, antibiotics, and predation. Biofilm formation is a major bacterial persistence factor of great concern in the clinic and the food industry. Many tested strains formed strong biofilms, and especially strains such as the heat-resistant, MDR strain FAM21845 may pose a serious issue for food production. Strong biofilm formation combined with diverse resistances (some encoded on conjugative plasmids) may allow for increased persistence, coselection, and possible transfer of these resistance factors. Horizontal gene transfer may conceivably occur in the food production setting or the gastrointestinal tract after consumption.

scholarly journals Surface conditioning with Escherichia coli cell wall components can reduce biofilm formation by decreasing initial adhesion

2017 ◽  
Vol 3 (3) ◽  
pp. 613-628 ◽  
Author(s):  
Luciana C. Gomes ◽  
◽  
Joana M. R. Moreira ◽  
José D. P. Araújo ◽  
Filipe J. Mergulhão ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Biofilm Formation ◽  
Cell Wall Components ◽  
Surface Conditioning ◽  
Initial Adhesion ◽  
Wall Components

scholarly journals Genotypic and Phenotypic Characteristics Associated with Biofilm Formation by Human Clinical Escherichia coli Isolates of Different Pathotypes

2017 ◽  
Vol 83 (24) ◽  
Author(s):  
Juliane Schiebel ◽  
Alexander Böhm ◽  
Jörg Nitschke ◽  
Michał Burdukiewicz ◽  
Jörg Weinreich ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Polymeric Matrix ◽  
Bacterial Biofilm ◽  
Automated Image Analysis ◽  
Host Immune System ◽  
Content Type ◽  
E Coli ◽  
Initial Adhesion ◽  
K 12

ABSTRACT Bacterial biofilm formation is a widespread phenomenon and a complex process requiring a set of genes facilitating the initial adhesion, maturation, and production of the extracellular polymeric matrix and subsequent dispersal of bacteria. Most studies on Escherichia coli biofilm formation have investigated nonpathogenic E. coli K-12 strains. Due to the extensive focus on laboratory strains in most studies, there is poor information regarding biofilm formation by pathogenic E. coli isolates. In this study, we genotypically and phenotypically characterized 187 human clinical E. coli isolates representing various pathotypes (e.g., uropathogenic, enteropathogenic, and enteroaggregative E. coli). We investigated the presence of biofilm-associated genes (“genotype”) and phenotypically analyzed the isolates for motility and curli and cellulose production (“phenotype”). We developed a new screening method to examine the in vitro biofilm formation ability. In summary, we found a high prevalence of biofilm-associated genes. However, we could not detect a biofilm-associated gene or specific phenotype correlating with the biofilm formation ability. In contrast, we did identify an association of increased biofilm formation with a specific E. coli pathotype. Enteroaggregative E. coli (EAEC) was found to exhibit the highest capacity for biofilm formation. Using our image-based technology for the screening of biofilm formation, we demonstrated the characteristic biofilm formation pattern of EAEC, consisting of thick bacterial aggregates. In summary, our results highlight the fact that biofilm-promoting factors shown to be critical for biofilm formation in nonpathogenic strains do not reflect their impact in clinical isolates and that the ability of biofilm formation is a defined characteristic of EAEC. IMPORTANCE Bacterial biofilms are ubiquitous and consist of sessile bacterial cells surrounded by a self-produced extracellular polymeric matrix. They cause chronic and device-related infections due to their high resistance to antibiotics and the host immune system. In nonpathogenic Escherichia coli, cell surface components playing a pivotal role in biofilm formation are well known. In contrast, there is poor information for their role in biofilm formation of pathogenic isolates. Our study provides insights into the correlation of biofilm-associated genes or specific phenotypes with the biofilm formation ability of commensal and pathogenic E. coli. Additionally, we describe a newly developed method enabling qualitative biofilm analysis by automated image analysis, which is beneficial for high-throughput screenings. Our results help to establish a better understanding of E. coli biofilm formation.

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