Influences of superheated steam roasting on changes in sugar, amino acid and flavour active components of cocoa bean (Theobroma cacao )

2017 ◽  
Vol 97 (13) ◽  
pp. 4429-4437 ◽  
Author(s):  
Wahidu Zzaman ◽  
Rajeev Bhat ◽  
Tajul Aris Yang ◽  
Azhar Mat Easa
Keyword(s):  
Amino Acid ◽  
Theobroma Cacao ◽  
Superheated Steam ◽  
Cocoa Bean ◽  
Active Components

scholarly journals PEMANFAATAN LIMBAH KULIT ARI BIJI KAKAO (Theobroma cacao L) SEBAGAI SUMBER ANTIBAKTERI Streptococcus mutans. (Utilization of Cocoa Beans Epidermis Waste (Theobroma cacao L) as Antibacterial Streptococcus mutans).

2017 ◽  
Vol 12 (2) ◽  
pp. 7-20
Author(s):  
Medan Yumas
Keyword(s):  
Incubation Period ◽  
Streptococcus Mutans ◽  
Active Compound ◽  
Theobroma Cacao ◽  
Antibacterial Properties ◽  
Cocoa Bean ◽  
Active Components ◽  
Cocoa Beans ◽  
Active Compounds ◽  
Theobroma Cacao L

Epidermis of cocoa beans contain active compounds that are similar to the active compound in the cocoa pod husk. Epidermis of cocoa beans are suspected to contain active compounds such as  polyphenols, flavonoids, terpenoids/steroids, condensed or polymerized tannins such as catechins and anthocyanins. The bioactive compounds were known to have antibacterial properties on Streptococcus mutans types. The aim of this research were to produce natural active compounds from cocoa bean husk, which have antibacterial activity against Streptococcus mutans. Stages of the study consisted of powdering process of epidermis cocoa beans, extracting fat from epidermis cocoa beans powder, extracting of the antibacterial active compound, solvent evaporation of active compounds extracts from powdered cocoa beans husk, and testing of active compounds produced by Streptococcus mutans. Results showed that the minimum inhibitory concentration (MIC) of the cocoa bean husk extract againsts Streptococcus mutans on a half-day incubation period was 0.25% with an inhibitory diameter of 7.550 mm. While, a two- days incubation period was 0.25% with an inhibitory diameter of 9,900 mm and was bacteriostatic.Keywords: cocoa beans husk, maceration extraction, methanol, antibacterial, active components, Streptococcus mutans ABSTRAK. Kulit ari biji kakao mengandung senyawa aktif yang tidak berbeda jauh dengan kandungan senyawa aktif yang terdapat pada kulit buah kakao dan biji kakao itu sendiri. Kulit ari biji kakao diduga mengandung senyawa aktif polifenol, flavonoid, terpenoid/steroid, tanin terkondensasi atau terpolimerisasi seperti katekin dan antosianin. Senyawa-senyawa bioaktif tersebut diketahui memiliki sifat antibakteri jenis Streptococcus mutans.Tujuan penelitian ini adalah pemanfaatan kulit ari biji kakao sebagai sumber senyawa aktif yang memiliki aktivitas antibakteri terhadap bakteri Streptococcus mutans. Tahapan penelitian terdiri dari proses pembuatan bubuk kulit ari biji kakao, proses ekstraksi lemak dari bubuk kulit ari biji kakao, proses ekstraksi senyawa aktif antibakteri dari bubuk kulit ari biji kakao, proses penguapan pelarut ekstrak senyawa aktif dari bubuk kulit ari biji kakao, dan proses pengujian senyawa aktif yang dihasilkan terhadap Streptococcus mutans. Hasil penelitian menunjukkan bahwa ekstrak limbah kulit ari biji kakao berpotensi sebagai senyawa aktif antibakteri terhadap Streptococcus mutans. Konsentrasi hambat minimum (KHM) ekstrak kulit ari biji kakao terhadap bakteri Streptococcus mutans pada masa inkubasi satu setengah hari adalah 0,25% dengan diameter hambatan sebesar 7,550 mm dan masa inkubasi dua hari adalah 0,25% dengan diameter hambatan sebesar  9,900 mm dan bersifat bakteriostatik.  Kata kunci : kulit ari biji kakao, ekstraksi maserasi, metanol, antibakteri, komponen aktif, Streptococcus mutans

scholarly journals In Vitro Studies on the Antioxidant Property and Inhibition of α-Amylase, α-Glucosidase, and Angiotensin I-Converting Enzyme by Polyphenol-Rich Extracts from Cocoa (Theobroma cacao) Bean

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Ganiyu Oboh ◽  
Ayokunle O. Ademosun ◽  
Adedayo O. Ademiluyi ◽  
Olasunkanmi S. Omojokun ◽  
Esther E. Nwanna ◽  
...  
Keyword(s):  
Enzyme Activities ◽  
Theobroma Cacao ◽  
Antioxidant Property ◽  
Cocoa Bean ◽  
Dependent Manner ◽  
Converting Enzyme ◽  
Angiotensin I Converting Enzyme ◽  
Dose Dependent

Background. This study sought to investigate the antidiabetic and antihypertensive mechanisms of cocoa (Theobroma cacao) bean through inhibition of α-amylase, α-glucosidase, angiotensin-1 converting enzyme, and oxidative stress. Methodology. The total phenol and flavonoid contents of the water extractable phytochemicals from the powdered cocoa bean were determined and the effects of the extract on α-amylase, α-glucosidase, and angiotensin-1 converting enzyme activities were investigated in vitro. Furthermore, the radicals [1,1-diphenyl-2 picrylhydrazyl (DPPH), 2,2..-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), hydroxyl (OH), and nitric oxide (NO)] scavenging ability and ferric reducing antioxidant property of the extract were assessed. Results. The results revealed that the extract inhibited α-amylase (1.81 ± 0.22 mg/mL), α-glucosidase (1.84 ± 0.17 mg/mL), and angiotensin-1 converting enzyme (0.674 ± 0.06 mg/mL [lungs], 1.006 ± 0.08 mg/mL [heart]) activities in a dose-dependent manner and also showed dose-dependent radicals [DPPH (16.94 ± 1.34 mg/mL), NO (6.98 ± 0.886 mg/mL), OH (3.72 ± 0.26 mg/mL), and ABTS (15.7 ± 1.06 mmol/TEAC·g] scavenging ability. Conclusion. The inhibition of α-amylase, α-glucosidase, and angiotensin-1 converting enzyme activities by the cocoa bean extract could be part of the possible mechanism by which the extract could manage and/or prevent type-2 diabetes and hypertension.

scholarly journals The remineralization potential of cocoa (Theobroma cacao) bean extract to increase the enamel micro hardness

2017 ◽  
Vol 29 (2) ◽  
Author(s):  
Sulistianingsih S. ◽  
Irmaleny I. ◽  
Opik Taofik Hidayat
Keyword(s):  
Theobroma Cacao ◽  
Vickers Microhardness ◽  
Tooth Enamel ◽  
Cocoa Bean ◽  
Natural Material ◽  
Micro Hardness ◽  
Synthetic Material ◽  
Fluorine Substitution ◽  
Average Value ◽  
Mineral Deposition

Introduction: Remineralization is the process of returning mineral ions into a hydroxyapatite structure characterized by mineral deposition on the enamel surface. The presence of mineral deposition would affect the microhardness of tooth enamel. Fluorine, as the remineralization agent is known with side effects such as fluorosis. Cocoa bean extract contains theobromine that can be used as an alternative remineralization ingredients. The objectives were to determine enamel microhardness after remineralization using cocoa bean extract as natural material and to compare with fluorine as synthetic material. Methods: Thirty-six maxillary first premolar tooth crowns was cut and planted in the epoxy resin. Teeth were then immersed in demineralization solution at pH 4 for 6 hours. The sample were divided into 2 groups, 18 for the fluorine group and the remaining group of cocoa extract. Vickers microhardness test was used before treatment, both after demineralized and remineralized. Results: The value of enamel microhardness before treatment in the fluorine group was 376.17 VHN as average value and the cocoa extract group was 357.33 VHN. After demineralization in fluorine group was 268.13 VHN and cocoa extract group was 235.93 VHN. After remineralization in fluorine group and cocoa extract group, respectively, 321.08 VHN and 293.86 VHN. The results of the analysis revealed that the level of enamel microhardness after remineralization was not significantly different in both groups (p>0.05). Conclusions: The findings indicated the ability of cocoa extract to increase the microhardness of enamel and implies the potential as a fluorine substitution for remineralization.

Inoculum of the starter consortia and interactive metabolic process in enhancing quality of cocoa bean (Theobroma cacao) fermentation

LWT ◽  
2016 ◽  
Vol 65 ◽  
pp. 731-738 ◽  
Author(s):  
M.V.S. Sandhya ◽  
B.S. Yallappa ◽  
M.C. Varadaraj ◽  
J. Puranaik ◽  
L. Jaganmohan Rao ◽  
...  
Keyword(s):  
Theobroma Cacao ◽  
Metabolic Process ◽  
Cocoa Bean

scholarly journals PEMANFAATAN KULIT BIJI KAKAO (THEOBROMA CACAO L) MENJADI PRODUK COOKIES COKLAT

2019 ◽  
pp. 44-50 ◽  
Author(s):  
Jumriah Langkong ◽  
Meta Mahendradatta ◽  
Mulyati M Tahir ◽  
Nurlaila Abdullah ◽  
Marina Reski
Keyword(s):  
Moisture Content ◽  
Protein Content ◽  
Theobroma Cacao ◽  
Animal Feed ◽  
Economic Value ◽  
Fat Content ◽  
Ash Content ◽  
Cocoa Bean ◽  
Theobroma Cacao L ◽  
Process Product

Cocoa bean husk is one of the product of cocoa which is only utilized as animal feed and has low economic value. Economic value of cocoa bean husks can be enhanced by extracting the husk using methanol into powder and applied to a process product namely cookies. Cookies are types of biscuit which are made from soft dough, high in fat and relatively crisp. The purpose of this study was to determine the amount of powder that was added and its impact to the cookies product. The analysis was done for sensory test, moisture content, fat content and protein content. The result showed that water content ranged from 2.39% - 4.85%. Fat content ranged from 7.57% - 8.64%, and ash content ranged from 0.73% - 2.02%. In the organoleptic test, the color and odor which were preferred by the panelists was treatment A0 (control) whereas the taste and texture was treatment A1 (addition of 5% powder).

scholarly journals PARTIAL CHARACTERIZATION of lipase from COCOA BEANS (Theobroma cacao. L.) of clone PBC 159

2010 ◽  
Vol 8 (3) ◽  
pp. 448-453
Author(s):  
Ratna Agung Samsumaharto
Keyword(s):  
Theobroma Cacao ◽  
Cocoa Bean ◽  
Cocoa Beans ◽  
Ph Optimum ◽  
Nitrobenzoic Acid ◽  
Lipase Enzyme ◽  
Wide Range ◽  
Km Value ◽  
Theobroma Cacao L

A study was carried out to characterize the cocoa lipase from cocoa beans (Theobroma cacao, L.) of clone PBC 159. The optimum temperature of cocoa lipase was 30-40 °C and the pH optimum was 7.0-8.0. The moleculer weight of the lipase enzyme was in between 45-66 kDa. The results indicate that Km value for cocoa bean lipase was 2.63 mM, when trimyristin was used as a substrate. The incubation of cocoa bean lipase with triolein and tributyrin (as substrate) yielded Km of 11.24 and 35.71 mM, respectively. The Vmax value obtained from the incubation of the lipase with a wide range of substrates, including tributyrin, trimyristin and triolein, are expressed as µmole acid/min/mg protein for cocoa lipase. Vmax values decreased with the increase in the triacylglycerol chain-length, with Vmax values of 27.78, 13.16 and 11.63 µmole acid/min/mg protein when incubated with tributyrin, trimyristin and triolein, respectively. Inhibition of lipase occurred in the presence of diisopropyl flourophosphate, N-bromosuccinimide and 5,5-dithiobis-(-2-nitrobenzoic acid).   Keywords: characterization, lipase, cocoa beans

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