High Performance Chromatographic and Spectroscopic Analyses of Mycobacterial Waxes and Glycolipids

AbstractTuliposides (Pos) are major defensive secondary metabolites in tulip (genus Tulipa), having 4-hydroxy-2-methylenebutanoyl and/or (3S)-3,4-dihydroxy-2-methylenebutanoyl groups at the C-1 and/or C-6 positions of d-glucose. The acyl group at the C-6 position is converted to antimicrobial lactones, tulipalins, by tuliposide-converting enzymes (TCEs). In the course of a survey of tulip tissue extracts to identify novel Pos, we found a minute high-performance liquid chromatography peak that disappeared following the action of a TCE, and whose retention time differed from those of known Pos. Spectroscopic analyses of the purified compound, as well as its enzymatic degradation products, revealed its structure as 5″-O-(6-O-(4′-hydroxy-2′-methylenebutanoyl))-β-d-glucopyranosyl-(2″R)-2″-hydroxymethyl-4″-butyrolactone, which is a novel glucoside ester-type Pos. We gave this compound the trivial name ‘tuliposide G’ (PosG). PosG accumulated in bulbs, at markedly lower levels than 6-PosA (the major Pos in bulbs), but was not found in any other tissues. Quantification of PosG in bulbs of 52 types of tulip, including 30 cultivars (Tulipa gesneriana) and 22 wild Tulipa spp., resulted in the detection of PosG in 28 cultivars, while PosG was present only in three wild species belonging to the subgenus Tulipa, the same subgenus to which tulip cultivars belong, suggesting the potential usefulness of PosG as a chemotaxonomic marker in tulip.

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Isolation and identification of tuliposides D and F from tulip cultivars

Zeitschrift für Naturforschung C ◽

10.1515/znc-2019-0123 ◽

2020 ◽

Vol 75 (1-2) ◽

pp. 7-12 ◽

Cited By ~ 1

Author(s):

Taiji Nomura ◽

Shinjiro Ogita ◽

Yasuo Kato

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Secondary Metabolites ◽

High Performance ◽

The Other ◽

Isolation And Identification ◽

Tulipa Gesneriana ◽

Tissue Extracts ◽

Spectroscopic Analyses ◽

Hplc Peak

Abstract6-Tuliposides A (6-PosA) and B (6-PosB) are major defensive secondary metabolites in tulip cultivars (Tulipa gesneriana), having an acyl group at the C-6 position of d-glucose. Although some wild tulip species produce 1,6-diacyl-glucose type of Pos (PosD and PosF), as well as 6-PosA/B, they have not yet been isolated from tulip cultivars. Here, aiming at verifying the presence of PosD and PosF in tulip cultivars, tissue extracts of 25 cultivars were analyzed by high-performance liquid chromatography (HPLC). Although no HPLC peaks for PosD nor PosF were detected in most cultivars, we found two cultivars giving a minute HPLC peak for PosD and the other two cultivars giving that for PosF. PosD and PosF were then purified from petals of cultivar ‘Orca’ and from pistils of cultivar ‘Murasakizuisho’, respectively, and their identities were verified by spectroscopic analyses. This is the first report that substantiates the presence of 1,6-diacyl-glucose type of Pos in tulip cultivars.

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Coreolanceolins A–E, New Flavanones from the Flowers of Coreopsis lanceolate, and Their Antioxidant and Anti-Inflammatory Effects

Antioxidants ◽

10.3390/antiox9060539 ◽

2020 ◽

Vol 9 (6) ◽

pp. 539 ◽

Cited By ~ 2

Author(s):

Hyoung-Geun Kim ◽

Young Sung Jung ◽

Seon Min Oh ◽

Hyun-Ji Oh ◽

Jung-Hwan Ko ◽

...

Keyword(s):

High Performance ◽

Radical Scavenging ◽

Reversed Phase ◽

Raw 264.7 Cells ◽

Flavonoid Content ◽

Spectroscopic Analyses ◽

Water Fractions ◽

Preliminary Study ◽

Organic Fractions ◽

Anti Inflammatory

(1) Background: Many flavonoids derived from natural sources have been reported to exhibit antioxidant and anti-inflammatory effects. Our preliminary study suggested that Coreopsis lanceolata flowers (CLFs) include high flavonoid content; (2) Methods: CLFs were extracted in 80% (v/v) aqueous methanol and fractionated into ethyl acetate, n-butanol, and water fractions. Repeated column chromatographies for the organic fractions led to the isolation of seven flavanones. Quantitative analysis of the flavanones was carried out using reversed-phase high-performance liquid chromatography. All flavanones were evaluated for their antioxidant and pro-inflammatory inhibition effects; (3) Results: Spectroscopic analyses revealed the chemical structure of five new flavanones, coreolanceolins A–E, and two known ones. The content of the seven flavanones in extracts were determined from 0.8 ± 0.1 to 38.8 ± 0.3 mg/g. All flavanones showed radical scavenging activities (respectively 104.3 ± 1.9 to 20.5 ± 0.3 mg vitamin C equivalents (VCE)/100 mg and 1278.6 ± 26.8 to 325.6 ± 0.2 mg VCE/100 mg) in the DPPH and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays and recovery activities in Caco-2 (59.7 to 41.1%), RAW264.7 (87.8 to 56.0%), and PC-12 (100.5 to 69.9%) cells against reactive oxygen species. Furthermore, all flavanones suppressed nitric oxide production (99.5% to 37.3%) and reduced iNOS and COX-2 expression in lipopolysaccharide-treated RAW 264.7 cells; (4) Conclusions: Five new and two known flavanones were isolated from CLF, and most of them showed high antioxidant and pro-inflammatory inhibition effects.

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INFLUENCE OF DIANILINE CROSS-LINKING SYSTEMS ON THE STRUCTURE, CURING MECHANISM, AND PROPERTIES OF POLYACRYLICESTER ELASTOMER

Rubber Chemistry and Technology ◽

10.5254/rct.18.81530 ◽

2018 ◽

Vol 91 (4) ◽

pp. 729-750 ◽

Cited By ~ 1

Author(s):

Tuhin Saha ◽

Anil K. Bhowmick ◽

Takeshi Oda ◽

Toshiaki Miyauchi ◽

Nobuhiko Fujii

Keyword(s):

Mechanical Properties ◽

Photoelectron Spectroscopy ◽

High Performance ◽

Density Functional ◽

Functional Theory ◽

Spectroscopic Analyses ◽

Curing Mechanism ◽

Temperature Superposition ◽

Kinetics Of ◽

Kinetics Of Vulcanization

ABSTRACT To develop high-performance polyacrylicester (ACM) elastomeric components with higher scorch safety and superior thermal and mechanical properties, we replaced aliphatic diamine curatives with aromatic dianiline curatives. The influence of dianiline curatives 4,4′-(4,4′-isopropylidenediphenyl-1,1′-diyldioxy)dianiline, 4,4′-(hexafluoroisopropylidene)bis(p-phenyleneoxy)dianiline, and 4,4′-(1,1′-biphenyl-4,4′-diyldioxy)dianiline on the network structures and thermal, dynamic mechanical, and mechanical properties of ACM vulcanizates was investigated. The kinetics of vulcanization was analyzed for different dianiline curatives, with the use of rheometer curves. To understand the electronic properties and study the relation between chemical structure and reactivity, density functional theory was used. The time–temperature superposition principal was used to evaluate the activation energy for degradation of cross-linked samples. Finally, the curing mechanism of ACM in the presence of dianiline curative was studied with X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. These spectroscopic analyses suggested that the reaction mechanism took place via two steps: the first step was formation of the amide linkage and the second step was formation of imide linkages.

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Active Monoterpene Ketones Isolated from Rosmarinus officinalis with Fumigant and Contact Action against Tyrophagus putrescentiae (Schrank)

Journal of Food Protection ◽

10.4315/0362-028x.jfp-14-087 ◽

2014 ◽

Vol 77 (8) ◽

pp. 1355-1360 ◽

Cited By ~ 6

Author(s):

JU-HYUN JEON ◽

JUN-HWAN PARK ◽

NAMHYUN CHUNG ◽

HOI-SEON LEE

Keyword(s):

High Performance ◽

Active Material ◽

Rosmarinus Officinalis ◽

Contact Toxicity ◽

Fumigant Toxicity ◽

Tyrophagus Putrescentiae ◽

Toxicity Bioassay ◽

Benzyl Benzoate ◽

Potential Control ◽

Spectroscopic Analyses

The acaricidal activities of an active material derived from Rosmarinus officinalis oil and its relative monoterpene ketones were determined using fumigant and contact toxicity bioassays against Tyrophagus putrescentiae and were compared with that of a commercial acaricide (benzyl benzoate). The active component of R. officinalis oil, isolated by silica gel column chromatography and high-performance liquid chromatography, was identified as camphor, based on various spectroscopic analyses. In the fumigant toxicity bioassay, camphor (2.25 μg/cm3) was 5.58 times more active than benzyl benzoate (12.56 μg/cm3) against T. putrescentiae, followed by (+)-camphor (3.89 μg/cm3) and (−)-camphor (5.61 μg/cm3). In the contact toxicity bioassay, camphor (1.34 μg/cm2) was 6.74 times more toxic than benzyl benzoate (9.03 μg/cm2) against T. putrescentiae, followed by (+)-camphor (2.23 μg/cm2) and (−)-camphor (2.94 μg/cm2). These results indicate that camphor and its derivatives are very useful as potential control agents against stored food mites regardless of the application method.

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Investigating the impact of weathering and indoor aging on wood acidity using spectroscopic analyses

BioResources ◽

10.15376/biores.15.3.6506-6525 ◽

2020 ◽

Vol 15 (3) ◽

pp. 6506-6525

Author(s):

Safa Abdel-Kader Mohamed Hamed ◽

Mohamed Z. M. Salem ◽

Hayssam M. Ali ◽

Kareem Mohamed El-Sayed Ahmed

Keyword(s):

Environmental Conditions ◽

High Performance ◽

Indoor Environment ◽

Outdoor Environment ◽

Spectroscopic Analyses ◽

Polluted Atmosphere ◽

Outdoor Environments ◽

Wood Deterioration ◽

New Perspective ◽

The Impact

A new perspective on the effect of unconditioned indoor (especially storage areas) and outdoor environments on wood acidity is provided in this work. A comparison between the quantity and types of the organic acids formed in the unconditioned indoor environment and different outdoor environments was made. Moreover, the acidity of some wood samples due to different environmental conditions was determined using a pH meter and high-performance liquid chromatography (HPLC). Fourier transform infrared (FTIR) was used to detect the changes in wood components at the molecular level due to environmental conditions. The results suggest that the unconditioned indoor environment was more aggressive than the outdoor environment with respect to wood deterioration. The polluted atmosphere increased the wood acidity and motivated polysaccharide breakdown.

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A High Performance Energy Analyzer for Use in Electron Scanning Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100061975 ◽

1969 ◽

Vol 27 ◽

pp. 14-15

Author(s):

A. V. Crewe ◽

M. Isaacson ◽

D. Johnson

Keyword(s):

High Performance ◽

Vertical Plane ◽

Median Plane ◽

Electron Gun ◽

Uniform Field ◽

Loss Mechanism ◽

Electron Scanning Microscopy ◽

Sector Type ◽

Double Focusing ◽

Electron Energy Loss

A double focusing magnetic spectrometer has been constructed for use with a field emission electron gun scanning microscope in order to study the electron energy loss mechanism in thin specimens. It is of the uniform field sector type with curved pole pieces. The shape of the pole pieces is determined by requiring that all particles be focused to a point at the image slit (point 1). The resultant shape gives perfect focusing in the median plane (Fig. 1) and first order focusing in the vertical plane (Fig. 2).

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Vacuum System to Minimize the Specimen Contamination of High-Performance EM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100077967 ◽

1977 ◽

Vol 35 ◽

pp. 68-69

Author(s):

N. Yoshimura ◽

K. Shirota ◽

T. Etoh

Keyword(s):

Electron Microscope ◽

High Speed ◽

High Performance ◽

High Vacuum ◽

Vacuum System ◽

Pump System ◽

Pumping System ◽

Diffusion Pump ◽

Almost All ◽

Cascade Type

One of the most important requirements for a high-performance EM, especially an analytical EM using a fine beam probe, is to prevent specimen contamination by providing a clean high vacuum in the vicinity of the specimen. However, in almost all commercial EMs, the pressure in the vicinity of the specimen under observation is usually more than ten times higher than the pressure measured at the punping line. The EM column inevitably requires the use of greased Viton O-rings for fine movement, and specimens and films need to be exchanged frequently and several attachments may also be exchanged. For these reasons, a high speed pumping system, as well as a clean vacuum system, is now required. A newly developed electron microscope, the JEM-100CX features clean high vacuum in the vicinity of the specimen, realized by the use of a CASCADE type diffusion pump system which has been essentially improved over its predeces- sorD employed on the JEM-100C.

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Use of the Magnetostatic Analogue In Electromagnetic Lens Engineering

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068795 ◽

1970 ◽

Vol 28 ◽

pp. 360-361

Author(s):

John W. Coleman

Keyword(s):

Boundary Conditions ◽

High Performance ◽

Force Fields ◽

Optical Design ◽

Direct Conversion ◽

Design Engineering ◽

Design Data ◽

Scalar Potentials ◽

Geometric Elements ◽

At Will

In the design engineering of high performance electromagnetic lenses, the direct conversion of electron optical design data into drawings for reliable hardware is oftentimes difficult, especially in terms of how to mount parts to each other, how to tolerance dimensions, and how to specify finishes. An answer to this is in the use of magnetostatic analytics, corresponding to boundary conditions for the optical design. With such models, the magnetostatic force on a test pole along the axis may be examined, and in this way one may obtain priority listings for holding dimensions, relieving stresses, etc..The development of magnetostatic models most easily proceeds from the derivation of scalar potentials of separate geometric elements. These potentials can then be conbined at will because of the superposition characteristic of conservative force fields.

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Prospects for convergent beam electron diffraction with a 200kV cold field-emission transmission electron microscope

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100086635 ◽

1991 ◽

Vol 49 ◽

pp. 466-467

Author(s):

J W Steeds ◽

R Vincent

Keyword(s):

Field Emission ◽

High Performance ◽

Small Diameter ◽

Convergent Beam Electron Diffraction ◽

Zone Axis ◽

Medium Voltage ◽

Transmission Electron ◽

Good Crystallinity ◽

Special Modification ◽

Convergent Beam

We review the analytical powers which will become more widely available as medium voltage (200-300kV) TEMs with facilities for CBED on a nanometre scale come onto the market. Of course, high performance cold field emission STEMs have now been in operation for about twenty years, but it is only in relatively few laboratories that special modification has permitted the performance of CBED experiments. Most notable amongst these pioneering projects is the work in Arizona by Cowley and Spence and, more recently, that in Cambridge by Rodenburg and McMullan.There are a large number of potential advantages of a high intensity, small diameter, focussed probe. We discuss first the advantages for probes larger than the projected unit cell of the crystal under investigation. In this situation we are able to perform CBED on local regions of good crystallinity. Zone axis patterns often contain information which is very sensitive to thickness changes as small as 5nm. In conventional CBED, with a lOnm source, it is very likely that the information will be degraded by thickness averaging within the illuminated area.

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