Abstract
Wound healing is one of the utmost medical issues in human and veterinary medicine, which explains the urgent need for developing new agents that possess wound-healing activities. The present study aimed to assess the effectiveness of green and chemical ZnO-NPs for wound healing. ZnO-NPs (chemical and green using Lawsonia inermis leaf extract) were synthesized and characterized by XRD; FTIR, and HRTEM. The gels containing the nanomaterials were prepared and inspected. Forty-five albino rats were divided into three groups, the control group was treated with normal saline 0.9 % and the other two groups were treated with gels containing green and chemical ZnO-NPs, respectively. On the 3rd, 7th, 14th, and 21st dpt, the wounds were clinical and histologically examined. Both nanomaterials have good crystallinity and high purity, but green ZnO-NPs have a longer nanowire length and diameter than chemical ZnO-NPs. The formed gels were highly viscous with a pH of 6.5 to 7. Both prepared gels showed clinical improvements, such as a decrease in WSA and WSA%, increase in WC%, and reduced healing time (P < 0.05) in both treated groups when compared with control group. The histological scoring of this study showed that the epithelization score was significantly higher at 21st dpt in treated groups than in the control group (P < 0.05), but the vasculature, necrosis, connective tissue formation, and collagen synthesis scores were mostly similar. The green and chemical ZnO-NPs gels showed promising wound healing properties however, the LI mediated-ZnO-NPs were more effective.