A survey of aflatoxin B1 and total aflatoxin contamination in baby food, peanut and corn products sold at retail in Indonesia analysed by ELISA and HPLC

2004 ◽  
Vol 20 (2) ◽  
pp. 51-58 ◽  
Author(s):  
E. Razzazi-Fazeli ◽  
C. T. Noviandi ◽  
S. Porasuphatana ◽  
A. Agus ◽  
J. Böhm
Keyword(s):  
Aflatoxin B1 ◽  
Aflatoxin Contamination ◽  
Baby Food ◽  
Total Aflatoxin ◽  
Corn Products

scholarly journals Changes in Fungal Population and Aflatoxin Levels and Assessment of Major Aflatoxin Types in Stored Peanuts (Arachis hypogaea Linnaeus)

2013 ◽  
Vol 2 (5) ◽  
pp. 10 ◽  
Author(s):  
John Maina Wagacha ◽  
Charity K. Mutegi ◽  
Maria E. Christie ◽  
Lucy W. Karanja ◽  
Job Kimani
Keyword(s):  
Moisture Content ◽  
Aflatoxin B1 ◽  
Fungal Pathogens ◽  
Aflatoxin Contamination ◽  
Fungal Population ◽  
Total Aflatoxin ◽  
Regulatory Standards ◽  
Polyethylene Bags ◽  
Jute Bags ◽  
The Eu

<p>Peanut kernels of Homabay Local, Valencia Red, ICGV-SM 12991 and ICGV-SM 99568 cultivars were stored for six months in jute, polypropylene and polyethylene bags to assess the effect of storage bags, temperature and R.H. on fungal population and aflatoxin contamination. Moisture content (M.C.), fungal population and aflatoxin levels were determined before storage and after every 30 days during storage. Isolates of <em>Aspergillus flavus</em> and <em>A. parasiticus</em> were assayed for production of aflatoxin B1, B2, G1 and G2. The correlation between MC, population of <em>A. flavus</em> and <em>A. parasiticus</em> and aflatoxin levels in peanuts was also determined. Six fungal pathogens were commonly isolated from the peanut samples and occurred as follows in decreasing order: <em>Penicillium</em> spp. (106.6 CFU/g), <em>A. flavus</em> L-strain (4.8 CFU/g), <em>A. flavus</em> S-strain (2.9 CFU/g), <em>A. niger </em>(2.6 CFU/g), <em>A. parasiticus </em>(1.7 CFU/g) and <em>A. tamarii </em>(0.2 CFU/g). The overall population of <em>A. flavus</em> L-strain was 66% higher than that of <em>A. flavus</em> S-strain. Ninety one percent of <em>A. flavus</em> and <em>A. parasiticus</em> isolates produced at least one of the four aflatoxin types assayed, with 36% producing aflatoxin B1. Total aflatoxin levels ranged from 0 - 47.8 µg/kg with samples stored in polyethylene and jute bags being the most and least contaminated, respectively. Eighty nine percent and 97% of the peanut samples met the EU (? 4 µg/kg) and Kenyan (? 10 µg/kg) regulatory standards for total aflatoxin, respectively. Peanuts should be adequately dried to safe moisture level and immediately packaged in a container - preferably jute bags - which will not promote critical increases in fungal population and aflatoxin contamination.</p>

scholarly journals Comparison of Three Methods for Determining Aflatoxins in Melon Seeds

1996 ◽  
Vol 79 (6) ◽  
pp. 1330-1335 ◽  
Author(s):  
Vincent P Diprossimo ◽  
Emil G Malek
Keyword(s):  
Aflatoxin B1 ◽  
Aflatoxin Contamination ◽  
The Other ◽  
Contamination Level ◽  
Total Aflatoxin ◽  
The Third ◽  
Acetone Water ◽  
Melon Seeds ◽  
Layer Chromatography ◽  
Better Than

Abstract The suitability of 3 methods for determining aflatoxins in melon seeds was examined. The first 2 are the Contaminants Branch (CB) method and the Best Foods (BF) method, both official methods for determining aflatoxins in peanuts and peanut products. The third method, the modified CB method–Rapid Modification of the Cottonseed (CB-RCSMod) method, devised in this work, was derived by combining steps from the CB method and the Rapid Modification of the Cottonseed method. The CB method was superior to the other 2 methods for quantitation of aflatoxins. It gave better recoveries and cleaner extracts that exhibit less fluorescent interference for thin-layer chromatography (TLC) than the BF method. Also, its solvent efficiency was better than that of the CB-RCS-Mod method. With the CB method, recoveries from spiked samples were 85.0% for aflatoxin B1 and 90.0% for anatoxin B2. Recoveries of G1 aflatoxins were more variable, averaging 90.0% for aflatoxin d and 72.5% for aflatoxin G2. Total aflatoxin recovery was 86.5% for the CB method. At a low aflatoxin contamination level (8 μg B1/kg sample), aflatoxin B1 was detectable by the CB method but not by the BF method. Detection of aflatoxins in BF method sample extracts by TLC was not improved by the use of chloroform–acetone–water (88 + 12 + 1), benzene–ethanol–water, or ether–methanol–water (96 + 3 +1) in place of the standard chloroform–acetone (88 + 12) developer. Use of ether–methanol–water (96 + 3 + 1) for detecting aflatoxins by TLC in the CB method extracts increased interference compared with the standard chloroform–acetone (88 + 12) developer.

scholarly journals Occurrence and Distribution of Aflatoxin in Maize from Selected Counties, Eastern Region, Kenya

2020 ◽  
Vol 3 (2) ◽  
pp. 7-20
Author(s):  
Nicholas M. Jacob ◽  
Shem O. Wandiga ◽  
David K. Kariuki ◽  
Vincent O. Madadi
Keyword(s):  
Aflatoxin B1 ◽  
High Performance ◽  
Aflatoxin Contamination ◽  
Human Consumption ◽  
Eastern Region ◽  
Policy And Practice ◽  
Fluorescence Detector ◽  
Total Aflatoxin ◽  
Mean Values ◽  
Maize Sample

Purpose: The study aimed to assess the occurrence and distribution of aflatoxin contamination on dry maize in different types of stores in Meru, Embu, Isiolo, Makueni and Machakos Counties of Eastern region of Kenya.Methodology: Automatic spear sampler was used to collect maize samples from each bag at even intervals. 280 maize samples were collected from 29 stores in five Counties. 100 g of each maize sample was ground, resampled into 50g, blended, extracted, centrifuged, filtered and a quantified for Aflatoxin B1, B2, G1 and G2. Samples were prepared and extracted with methanol/water. The bulky of the samples were analyzed with enzyme-linked immunoassay test kits. Confirmation of positive samples was done with high performance liquid chromatography (HPLC) coupled with fluorescence detector.  Data analysis was done with SPSS and Microsoft excel.Findings: Maize samples from Counties in eastern region of Kenya had significantly high levels of (93.10%) aflatoxin contamination. The mean values for aflatoxin B1, B2, G1 and G2 were: 50.08± 4.42, 17.26±1.08, 30.17±2.06 and 10.54± 1.52 (ng/g) in that order. Only nine samples had total aflatoxin within the accepted limit for human consumption of 15 ng/g. The highest total aflatoxin contamination recorded was 198.45ng/g in Makueni county and the lowest recorded was 8.76ng/g in Embu county. Makueni and Embu had mean values for aflatoxin B1, B2, G1 and G2 being (83.07±7.53, 22.15± 1.36, 49.38±3.11, 20.52± 0.70 ng/g) and (18.71 ±2.63, 8.07 ±0.64, 17.02 ±1.38, 8.86 ±1.62 ng/g). Makueni NCPB depot had the highest mean contamination with aflatoxin B1 of 92.67± 5.78 ng/g and Embu had the lowest with 6.26 ± 4.14 ng/g. All the county markets recorded high aflatoxin B1 contamination with exception of Embu county which had a mean of 4.0 ±0.84, Makueni (83.67± 10.42 ng/g), Isiolo (51.27± 32.29 ng/g), Meru (46.02± 23.88 ng/g) and Machakos (36.34± 26.27 ng/g). The stores had aflatoxin load varied from on store to the other and county to county.Unique contribution to theory, policy and practice: The counties in the region had high occurrence and distribution of aflatoxin B1, B2, G1 and G2 in maize in all stores where samples were picked. Location for maize stores should be in areas with low levels of carbon dioxide because mycotoxins are produced under aerobic conditions. The design for maize threshing machines should not course shocks, breakage and cracks on maize grains to decrease chances of mycotoxins infestation during their storage.

Occurrence of Aflatoxins, Ochratoxin A, and Fumonisins in Retail Foods in Japan

2006 ◽  
Vol 69 (6) ◽  
pp. 1365-1370 ◽  
Author(s):  
YOSHIKO SUGITA-KONISHI ◽  
MASAHIRO NAKAJIMA ◽  
SETSUKO TABATA ◽  
EIICHI ISHIKURO ◽  
TOSHITSUGU TANAKA ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
High Performance ◽  
Peanut Butter ◽  
Fumonisin B1 ◽  
Aflatoxin Contamination ◽  
Coffee Beans ◽  
Corn Products ◽  
Buckwheat Flour

We conducted a survey of aflatoxin B1, B2, G1, and G2, ochratoxin A, and fumonisin B1, B2, and B3 contamination in various foods on the retail market in Japan in 2004 and 2005. The mycotoxins were analyzed by high-performance liquid chromatography, liquid chromatography–mass spectrometry, or high-performance thin-layer chromatography. Aflatoxins were detected in 10 of 21 peanut butter samples; the highest concentration of aflatoxin B1 was 2.59 μg/kg. Aflatoxin contamination was not found in corn products, corn, peanuts, buckwheat flour, dried buckwheat noodles, rice, or sesame oil. Ochratoxin A was detected in oatmeal, wheat flour, rye, buckwheat flour, green coffee beans, roasted coffee beans, raisins, beer, and wine but not in rice or corn products. Ochratoxin A concentrations in contaminated samples were below 0.8 μg/kg. Fumonisins were detected in popcorn, frozen corn, corn flakes, and corn grits. The highest concentrations of fumonisins B1, B2, and B3 in these samples were 354.0, 94.0, and 64.0 μg/kg, respectively.

Production of Aflatoxin in Cocoa Beans

1979 ◽  
Vol 62 (5) ◽  
pp. 1076-1079 ◽  
Author(s):  
Lawrence M Lenovich ◽  
W Jeffrey Hurst
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Ivory Coast ◽  
Aspergillus Parasiticus ◽  
Aflatoxin Production ◽  
Growth Conditions ◽  
Aflatoxin Contamination ◽  
Cocoa Beans ◽  
Total Aflatoxin ◽  
Substrate Variation

Abstract Aflatoxin was produced in both non-autoclaved and autoclaved Ivory Coast cocoa beans inoculated with Aspergillus parasiticus NRRL 2999 under optimum laboratory growth conditions. Total aflatoxin levels ranged from 213 to 5597 ng/g substrate. Aflatoxin was quantitated by using high pressure liquid chromatography (HPLC). Raw, non-autoclaved cocoa beans, also inoculated with aspergilli, produced 6359 ng aflatoxin/g substrate. Variation in aflatoxin production between bean varieties was observed. Total aflatoxin levels of 10,446 and 23,076 ng/g substrate were obtained on Ivory Coast beans inoculated with A. parasiticus NRRL 2999 and NRRL 3240, respectively. Aflatoxin production on Trinidad and Malaysian beans was 28 and 65 ng aflatoxin/g substrate. These data support previously reported low level natural aflatoxin contamination in cocoa.

scholarly journals Detection of Aflatoxin in Poultry Feed and Feed Materials through Immuno Based Assay from Different Poultry Farms and Feed Factories in Bangladesh

2019 ◽  
Vol 35 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Mahbuba Akter Lubna ◽  
Mita Debnath ◽  
Farzana Hossaini
Keyword(s):  
Health Risk ◽  
Positive Result ◽  
Mean Value ◽  
Aflatoxin Contamination ◽  
Control Measures ◽  
Poultry Feed ◽  
Enzyme Linked Immunosorbent Assay ◽  
Total Aflatoxin ◽  
Poultry Farms ◽  
Aflatoxin Concentration

Current study investigated the occurrence of aflatoxin contamination in poultry feed and feed materials in different poultry farms and feed factories in Bangladesh. A total of 100 samples of finished feed and raw feed materials were collected and tested through direct competitive Enzyme-Linked Immunosorbent Assay (ELISA) for total aflatoxin detection. Overall, 97% samples (n=97/100) in our study, were found positive for aflatoxin contamination. Among finished feed categories, layer grower feed contained highest level of aflatoxin with a mean value of 21.64 ppb whereas layer feed was less susceptible for aflatoxin contamination (mean value 9.49 ppb). Between raw feed materials, maize samples were highly contaminated (n=15/15, 100%) with aflatoxin while 86.67% soybean samples showed positive result. Twenty one percent (21%) of the samples in our study contained aflatoxin concentration more than the acceptable limit employed by USFDA and many other countries which might pose severe health risk to poultry and human consumer. Proper surveillance and immediate control measures should be taken to ensure safe poultry feed and feed materials. Bangladesh J Microbiol, Volume 35 Number 1 June 2018, pp 75-78

scholarly journals A Case for Regular Aflatoxin Monitoring in Peanut Butter in Sub-Saharan Africa: Lessons from a 3-Year Survey in Zambia

2016 ◽  
Vol 79 (5) ◽  
pp. 795-800 ◽  
Author(s):  
SAMUEL M. C. NJOROGE ◽  
LIMBIKANI MATUMBA ◽  
KENNEDY KANENGA ◽  
MOSES SIAMBI ◽  
FARID WALIYAR ◽  
...  
Keyword(s):  
South Africa ◽  
Aflatoxin B1 ◽  
Peanut Butter ◽  
Aflatoxin Contamination ◽  
Comprehensive Analysis ◽  
Sub Saharan Africa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Contamination Level ◽  
Batch Number ◽  
Sub Saharan

ABSTRACT A 3-year comprehensive analysis of aflatoxin contamination in peanut butter was conducted in Zambia, sub-Saharan Africa. The study analyzed 954 containers of 24 local and imported peanut butter brands collected from shops in Chipata, Mambwe, Petauke, Katete, and Nyimba districts and also in Lusaka from 2012 to 2014. For analysis, a sample included six containers of a single brand, from the same processing batch number and the same shop. Each container was quantitatively analyzed for aflatoxin B1 (AFB1) in six replicates by using competitive enzyme-linked immunosorbent assay; thus, aflatoxin contamination level of a given sample was derived from an average of 36 test values. Results showed that 73% of the brands tested in 2012 were contaminated with AFB1 levels &gt;20 μg/kg and ranged up to 130 μg/kg. In 2013, 80% of the brands were contaminated with AFB1 levels &gt;20 μg/kg and ranged up to 10,740 μg/kg. Compared with brand data from 2012 and 2013, fewer brands in 2014, i.e., 53%, had aflatoxin B1 levels &gt;20 μg/kg and ranged up to 1,000 μg/kg. Of the eight brands tested repeatedly across the 3-year period, none consistently averaged ≤20 μg/kg. Our survey clearly demonstrates the regular occurrence of high levels of AF B1 in peanut butter in Zambia. Considering that some of the brands tested originated from neighboring countries such as Malawi, Zimbabwe, and South Africa, the current findings provide a sub-Saharan regional perspective regarding the safety of peanut butter.

Extracts of Agave americana inhibit aflatoxin production in Aspergillus parasiticus

2011 ◽  
Vol 4 (1) ◽  
pp. 37-42 ◽  
Author(s):  
A. Rosas-Taraco ◽  
E. Sanchez ◽  
S. García ◽  
N. Heredia ◽  
D. Bhatnagar
Keyword(s):  
Aspergillus Parasiticus ◽  
Public Awareness ◽  
Aflatoxin Production ◽  
Aflatoxin Contamination ◽  
Aqueous Extracts ◽  
Total Aflatoxin ◽  
Toxigenic Fungi ◽  
Agave Americana ◽  
Acid Accumulation ◽  
Norsolorinic Acid

Toxigenic fungi invade crops prior to harvest as well as during storage and produce harmful, even carcinogenic toxins such as aflatoxins. Since consumers demand safe commodities, and due to enhanced public awareness of the dangers of many synthetic fungicides, the importance of investigating alternative, natural products to control these toxigenic fungi is clear. This study investigated the effect of aqueous extracts of Agave americana on growth, conidia and aflatoxin production. Aspergillus parasiticus strains SRRC 148, SRRC 143 (Su-1), and A. parasiticus SRRC 162, a mutant (nor-) that accumulates norsolorinic acid (NOR, an orange-coloured intermediate of the aflatoxin pathway), were first inoculated into Adye and Mateles liquid medium, then plant extracts were added, and incubated at 28 °C for 7 days. Aflatoxin and norsolorinic acid were assayed by HPLC and spectrophotometry, respectively. While the extract of A. americana stimulated growth of the studied fungi, conidiogenesis, norsolorinic acid accumulation (in the nor- mutant), and aflatoxin production were significantly affected. The reduction was produced by the extracts at concentrations higher than 5-10 mg/ml, where all types of total aflatoxin analysed (aflatoxins B1, B2, G1 and G2) were reduced from 64% to >99% in the whole culture, and a reduction of 75% of norsolorinic acid. The results of the present work indicate that extracts of A. americana may be promising safe alternatives to harmful fungicides for controlling aflatoxin contamination.

scholarly journals In Vitro Activity of Balanites aegyptiaca and Tamarindus indica Fruit Extracts on Growth and Aflatoxigenicity of Aspergillus flavus and A. parasiticus

2013 ◽  
Vol 2 (4) ◽  
pp. 68 ◽  
Author(s):  
Saifeldin Ahmed El-nagerabi ◽  
Abdulkadir E. Elshafie ◽  
Mohamed R. Elamin
Keyword(s):  
Aspergillus Flavus ◽  
Fungal Growth ◽  
Aflatoxin Contamination ◽  
Tamarindus Indica ◽  
Balanites Aegyptiaca ◽  
Total Aflatoxin ◽  
Animal Products ◽  
Fruit Extracts ◽  
Aflatoxin B

<p>Aflatoxin and especially aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) is a carcinogenic secondary metabolite synthesized by certain <em>Aspergillus </em>species. They contaminate natural and processed agricultural and animal products which render them unfit for consumption. The aim of this study was to evaluate the <em>in vitro</em> effects of <em>Balanites aegyptiaca</em> and <em>Tamarindus indica</em> fruit extracts on the growth and aflatoxin secretion of <em>Aspergillus flavus</em> (SQU21) and <em>A. parasiticus </em>(CBS921.7) strains. The two fruit extracts significantly (<em>P </em>&lt; 0.05) reduced aflatoxin and did not inhibit mycelial dry weights of the two <em>Aspergillus </em>strains. At different concentrations of balanites (2.5-10%), the inhibition of total aflatoxin was 49.9-84.8% for <em>A. flavus</em> (SQU21) and 32.1-84.4% for <em>A. parasiticus</em> (CBS921.7), whereas the inhibition of aflatoxin Bwas 38.2-81.4% and 32.8-80.6% for the two strains. Tamarind fruit extract (2.5-7.5%) caused 28.8-84.2% and 40.7-85.5% reductions in total aflatoxin and 37.1-83.5% and 33.9-85.9% in aflatoxin B for the two strains, respectively. None of these extracts inhibited the fungal growth or detoxified synthetic aflatoxin B<sub>1</sub>. We have concluded that these fruits contain various inhibitors to aflatoxin biosynthesis and secretion. Therefore, they can be used in combination as safe green biopreservatives to combat aflatoxin contamination of food.</p>

scholarly journals Aflatoxin B1 risk management in Parmigiano Reggiano dairy cow feed

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Giorgia Canestrari ◽  
Barbara Ricci ◽  
Valentina Pizzamiglio ◽  
Alberto Biancardi ◽  
Pierluigi Piazza ◽  
...  
Keyword(s):  
Risk Management ◽  
Aflatoxin B1 ◽  
Dairy Cow ◽  
Control Program ◽  
Aflatoxin Contamination ◽  
Quality Control Program ◽  
Control Procedures ◽  
Feed Samples ◽  
Parmigiano Reggiano ◽  
The Eu

This study investigated aflatoxin B1 (AFB1) contamination in dairy cow feed and the risk management of AFB1 content in concentrates undertaken by feed industries in the Parmigiano Reggiano area. Data on aflatoxin contamination risk management applied in 29 feed industries were collected and the AFB1 content of 70 feed samples was analyzed. Data were collected within the framework of a quality control program promoted by the Parmigiano Reggiano Consortium in 2013 and 2014. Audit results showed that the control procedures to prevent AFB1 contamination mainly focused on maize and its by-products. AFB1 concentration resulted lower than 5 ppb (legal EU limit) in all samples; in one out of 70 samples, AFB1 content was 3.8 ppb and in all the other samples it was lower than 3 ppb. Results showed that AFB1 risk management applied by Italian feed industries effectively monitors AFB1 levels in feed below the EU legal limit.

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