Comprehensive gene expression analysis of the NAC gene family under normal growth conditions, hormone treatment, and drought stress conditions in rice using near-isogenic lines (NILs) generated from crossing Aday Selection (drought tolerant) and IR64

Ovate family proteins (OFPs) are a family of plant growth regulators that play diverse roles in many aspects of physiological processes. OFPs have been characterized in various plant species including tomato, Arabidopsis, and rice. However, little is known about OFPs in woody species. Here, a total of 30 PtOFP genes were identified from the genome of Populus trichocarpa and were further grouped into four subfamilies based on their sequence similarities. Gene expression analysis indicated that some members of the PtOFP gene family displayed tissue/organ-specific patterns. Analysis of cis-acting elements in the promoter as well as gene expression by hormone treatment revealed putative involvement of PtOFPs in hormonal response. Furthermore, PtOFP1 (Potri.006G107700) was further experimentally demonstrated to act as a transcriptional repressor. Yeast two-hybrid assay showed physical interactions of PtOFP1 with other proteins, which suggests that they might function in various cellular processes by forming protein complexes. In addition, overexpression of PtOFP1 in Arabidopsis conferred enhanced tolerance to PEG-induced drought stress at seedling stage, as well as a higher survival rate than the wild type at mature stage. These results provide a systematic analysis of the Populus OFP gene family and lay a foundation for functional characterization of this gene family.

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Drought Tolerant near Isogenic Lines (NILs) of Pusa 44 Developed through Marker Assisted Introgression of qDTY2.1 and qDTY3.1 Enhances Yield under Reproductive Stage Drought Stress

Agriculture ◽

10.3390/agriculture11010064 ◽

2021 ◽

Vol 11 (1) ◽

pp. 64

Author(s):

Priyanka Dwivedi ◽

Naleeni Ramawat ◽

Gaurav Dhawan ◽

Subbaiyan Gopala Krishnan ◽

Kunnummal Kurungara Vinod ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Grain Quality ◽

Reproductive Stage ◽

Recurrent Parent ◽

Near Isogenic Lines ◽

Improvement Project ◽

Isogenic Lines ◽

Drought Tolerant ◽

Rice Improvement

Reproductive stage drought stress (RSDS) is detrimental for rice, which affects its productivity as well as grain quality. In the present study, we introgressed two major quantitative trait loci (QTLs), namely, qDTY2.1 and qDTY3.1, governing RSDS tolerance in a popular high yielding non-aromatic rice cultivar, Pusa 44, through marker-assisted backcross breeding (MABB). Pusa 44 is highly sensitive to RSDS, which restricts its cultivation across drought-prone environments. Foreground selection was carried out using markers, RM520 for qDTY3.1 and RM 521 for qDTY2.1. Background selection was achieved with 97 polymorphic SSR markers in tandem with phenotypic selection to achieve faster recurrent parent genome (RPG) recovery. Three successive backcrosses followed by three selfings aided RPG recoveries of 98.6% to 99.4% among 31 near isogenic lines (NILs). Fourteen NILs were found to be significantly superior in yield and grain quality under RSDS with higher drought tolerance efficiency (DTE) than Pusa 44. Among these, the evaluation of two promising NILs in the multilocational trial during Kharif 2019 showed that they were significantly superior to Pusa 44 under reproductive stage drought stress, while performing on par with Pusa 44 under normal irrigated conditions. These di-QTL pyramided drought-tolerant NILs are in the final stages of testing the All India Coordinated Rice Improvement Project varietal trials for cultivar release. Alternately, the elite drought-tolerant Pusa 44 NILs will serve as an invaluable source of drought tolerance in rice improvement.

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Response of Interspecific Hybrid Species of Manchurian Ash to MJ and NO Signals and Preliminary Study on the Formation Mechanism of Drought Resistance Advantage

10.21203/rs.3.rs-1147012/v1 ◽

2021 ◽

Author(s):

Yang Cao ◽

fei song ◽

Xingtang Zhao ◽

Liming He ◽

Yaguang Zhan

Keyword(s):

Gene Expression ◽

Drought Stress ◽

Drought Resistance ◽

Interspecific Hybrid ◽

Normal Growth ◽

Control Group ◽

Physiological Indicators ◽

Manchurian Ash ◽

In The Wild ◽

Set Up

Abstract Background: In this study, sodium nitrate (SNP, a donor of nitric oxide) and methyl jasmonate (MJ) were used as exogenous hormones. The experiment was conducted with the offspring (interspecific hybrid) D110 of ash and ash, and their respective parents (non-interspecific hybrid) D113 and 4-3 as experimental materials. The experiment set up three experimental groups of drought stress, exogenous hormone SNP and MJ, and a control group under normal growth (non-drought stress), to study the physiological indicators and gene expression of manchurian ash. Result: The results showed that under drought stress and exogenous application of hormone SNP or MJ, there were significant differences between hybrids and parents in plant growth, photosynthesis, defense enzyme activity, hormone content and gene expression.Conclusions: This experiment provides a new theoretical support for the existing hormone breeding methods of manchurian ash, which can improve the drought resistance of manchurian ash and increase its survival rate in the wild. Increasing the growth rate and breeding efficiency of manchurian ash brings new ideas.

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Gene expression profiling of Sinapis alba leaves under drought stress and rewatering growth conditions with Illumina deep sequencing

Molecular Biology Reports ◽

10.1007/s11033-011-1395-9 ◽

2011 ◽

Vol 39 (5) ◽

pp. 5851-5857 ◽

Cited By ~ 11

Author(s):

Cai-Hua Dong ◽

Chen Li ◽

Xiao-Hong Yan ◽

Shun-Mou Huang ◽

Jin-Yong Huang ◽

...

Keyword(s):

Gene Expression ◽

Drought Stress ◽

Gene Expression Profiling ◽

Deep Sequencing ◽

Expression Profiling ◽

Growth Conditions ◽

Sinapis Alba ◽

Illumina Deep Sequencing

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Transcript profiling and gene expression analysis under drought stress in Ziziphus nummularia (Burm.f.) Wright & Arn.

Molecular Biology Reports ◽

10.1007/s11033-018-4149-0 ◽

2018 ◽

Vol 45 (2) ◽

pp. 163-174 ◽

Cited By ~ 4

Author(s):

Radha Yadav ◽

Om Prakash Verma ◽

Jasdeep Chatrath Padaria

Keyword(s):

Gene Expression ◽

Drought Stress ◽

Expression Analysis ◽

Gene Expression Analysis ◽

Transcript Profiling ◽

Ziziphus Nummularia

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Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress

BMC Plant Biology ◽

10.1186/1471-2229-11-174 ◽

2011 ◽

Vol 11 (1) ◽

pp. 174 ◽

Cited By ~ 87

Author(s):

Ali Moumeni ◽

Kouji Satoh ◽

Hiroaki Kondoh ◽

Takayuki Asano ◽

Aeni Hosaka ◽

...

Keyword(s):

Comparative Analysis ◽

Drought Stress ◽

Near Isogenic Lines ◽

Root Transcriptome ◽

Isogenic Lines ◽

Drought Tolerant

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Antioxidant gene expression analysis and evaluation of total phenol content and oxygen-scavenging system in tea accessions under normal and drought stress conditions

BMC Plant Biology ◽

10.1186/s12870-021-03275-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Mehdi Rahimi ◽

Mojtaba Kordrostami ◽

Fereshteh Mohamadhasani ◽

Sanam Safaei Chaeikar

Keyword(s):

Gene Expression ◽

Antioxidant Activity ◽

Antioxidant Enzymes ◽

Drought Stress ◽

Expression Analysis ◽

Gene Expression Analysis ◽

Stress Conditions ◽

Total Phenol Content ◽

Phenol Content ◽

Oxygen Scavenging

Abstract Background Abiotic and biotic stresses induce oxidative processes in plant cells that this process starts with the production of ROSs which cause damage to the proteins. Therefore, plants have increased their antioxidant activity to defend against this oxidative stress to be able to handle stress better. In this research, 14 different tea accessions in a randomized complete block design with two replications were evaluated in two normal and drought stress conditions, and their antioxidant activity was measured by DPPH-free radicals’ assay and gene expression analysis. Results The results of gene expression analysis showed that the 100 and 399 accessions and Bazri cultivar had high values for most of the antioxidant enzymes, ascorbate peroxidase, superoxide dismutase, catalase, and peroxidase under drought stress conditions while the 278 and 276 accessions had the lowest amount of antioxidant enzymes in the same situation. Results showed that the IC50 of the BHT combination was 90.12 μg/ ml. Also, The IC50 of accessions ranged from 218 to 261 μg/ml and 201–264 μg/ml at normal and drought stress conditions, respectively. The 100 and 399 accessions showed the lowest IC50 under normal and drought stress conditions, while 278 and 276 accessions had the highest value for IC50. The antioxidant activity of tea accession extracts under normal conditions was ranged from 25 to 69% for accessions 278 and 100, respectively. While, the antioxidant activities of extracts under drought stress condition was 12 to 83% for accessions 276 and 100, respectively. So, according to the results, 100 and 399 accessions exhibited the least IC50 and more antioxidant activity under drought stress conditions and were identified as stress-tolerant accessions. However, 278 and 276 accessions did not show much antioxidant activity and were recognized as sensitive accessions under drought stress conditions. Conclusions These results demonstrate that total phenol content, antioxidant activity, and the oxygen-scavenging system can be used as a descriptor for identifying drought-tolerant accessions.

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Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice

10.21203/rs.3.rs-74931/v1 ◽

2020 ◽

Author(s):

Kai Liu ◽

Mingjuan Li ◽

Bin Zhang ◽

Yanchun Cui ◽

Xuming Yin ◽

...

Keyword(s):

Drought Stress ◽

Stress Tolerance ◽

Molecular Mechanisms ◽

Normal Growth ◽

Growth Conditions ◽

Growth Stages ◽

Grain Length ◽

Drought Stress Tolerance ◽

High Level ◽

Seed Setting Percentage

Abstract BackgroundGrain yield is a polygenic trait influenced by environmental and genetic interactions at all growth stages of the cereal plant. However, the molecular mechanisms responsible for coordinating the trade-off or cross-talk between these traits remain elusive.ResultsWe characterized the hitherto unknown function of four STRESS_tolerance and GRAIN_LENGTH (OsSGL) Poaceae ortholog genes, all encoding DUF1645 domain-containing proteins, in simultaneous regulation of grain length, grain weight, and drought stress-tolerance in rice. In normal growth conditions, the four ortholog genes were mainly expressed in the developing roots and panicles of the corresponding species. Over-expressing or heterologous high-level expressing Poaceae OsSGL ortholog genes conferred remarkably increased grain length, weight, and seed setting percentage, as well as significantly improved drought-stress tolerance in transgenic rice. Microscopical analysis also showed that the transgene expression promoted cell division and development. RNA-seq and qRT-PCR analyses revealed 73.8% (18,711) overlapped DEGs in all transgenic plants. Moreover, GO and KEGG analyses of different comparisons revealed that the key DEGs participating in drought stress-response belonged to hormone (especially auxin and cytokinin) pathways, and signaling processes were apparently affected in the young panicles. ConclusionTogether, these results suggest the four OsSGL orthologs perform a conserved function in regulating stress-tolerance and cell growth by acting via a hormone biosynthesis and signaling pathway. It may also induce a strategy for tailor-made crop yield improvement.

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Yellow nutsedge WRI3/4-like gene improves drought tolerance in Arabidopsis thaliana by promoting cuticular wax biosynthesis

10.21203/rs.2.18303/v2 ◽

2019 ◽

Author(s):

Chao Cheng ◽

Shutong Hu ◽

Yun Han ◽

Di Xia ◽

Bang-Lian Huang ◽

...

Keyword(s):

Fatty Acid ◽

Drought Stress ◽

Drought Tolerance ◽

Normal Growth ◽

Growth Conditions ◽

Cuticular Wax ◽

Expression Data ◽

Yellow Nutsedge ◽

Transgenic Lines ◽

Simulated Drought

Abstract Using RACE PCR, full length WRI1-like gene was amplified from yellow nutsedge. Conserved domain and phylogenetic analyses suggested it as WRI3/4-like gene. Tissue-specific expression data showed the highest expression in leaves, followed by roots while the lowest expression was detected in tuber. Transgenic Arabidopsis plants expressing nutsedge WRI3/4-like gene showed significantly improved tolerance to both PEG-simulated drought stress and real dehydration, compared with the wild type (WT). Under normal growth conditions, the expressions of key fatty acid biosynthesis genes was not significantly different between WT and transgenic lines, while the expressions of genes involved in cuticular wax biosynthesis was significantly higher in transgenic lines compared with the WT. The PEG-simulated drought stress did not induce any significant change in the expression of fatty acid and wax biosynthesis genes in WT plants, while the expression of fatty acid and wax biosynthesis genes was significantly increased in transgenic lines compared with WT as well as unstressed transgenic control. The expression of TAG1, the gene involved in triacylglycerol (TAG) accumulation, was significantly lower in the transgenic lines than that in the WT in normal growth conditions. Drought stress slightly decreased the expression of TAG1 in the WT, but significantly lowered it in transgenic lines compared with its unstressed transgenic control and WT. Consistent with gene expression data, the cuticular wax content in Arabidopsis leaves was significantly higher in the transgenic lines than in the WT, while the oil content was not significantly different. Our results indicated that WRI3/4-like gene from Cyperus esculentus improves drought tolerance in Arabidopsis probably by promoting cuticular wax biosynthesis and, hence, could be a valuable target for improving drought tolerance in crops through recombinant DNA technology.

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