Genome-wide identification and expression analysis of the SNARE genes in Foxtail millet (Setaria italica) reveals its roles in drought stress

2021 ◽  
Author(s):  
Hui Wang ◽  
Dongdong Hao ◽  
Xiaoxia Wang ◽  
Haiying Zhang ◽  
Pu Yang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Expression Analysis ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Genome Wide

scholarly journals Genome-Wide Analysis of MADS-Box Genes in Foxtail Millet (Setaria italica L.) and Functional Assessment of the Role of SiMADS51 in the Drought Stress Response

2021 ◽  
Vol 12 ◽  
Author(s):  
Wan Zhao ◽  
Li-Li Zhang ◽  
Zhao-Shi Xu ◽  
Liang Fu ◽  
Hong-Xi Pang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Survival Rates ◽  
Foxtail Millet ◽  
Negative Regulator ◽  
Setaria Italica ◽  
Mads Box ◽  
Drought Stress Tolerance ◽  
Genome Wide

MADS-box transcription factors play vital roles in multiple biological processes in plants. At present, a comprehensive investigation into the genome-wide identification and classification of MADS-box genes in foxtail millet (Setaria italica L.) has not been reported. In this study, we identified 72 MADS-box genes in the foxtail millet genome and give an overview of the phylogeny, chromosomal location, gene structures, and potential functions of the proteins encoded by these genes. We also found that the expression of 10 MIKC-type MADS-box genes was induced by abiotic stresses (PEG-6000 and NaCl) and exogenous hormones (ABA and GA), which suggests that these genes may play important regulatory roles in response to different stresses. Further studies showed that transgenic Arabidopsis and rice (Oryza sativa L.) plants overexpressing SiMADS51 had reduced drought stress tolerance as revealed by lower survival rates and poorer growth performance under drought stress conditions, which demonstrated that SiMADS51 is a negative regulator of drought stress tolerance in plants. Moreover, expression of some stress-related genes were down-regulated in the SiMADS51-overexpressing plants. The results of our study provide an overall picture of the MADS-box gene family in foxtail millet and establish a foundation for further research on the mechanisms of action of MADS-box proteins with respect to abiotic stresses.

scholarly journals Genome-wide identification, characterization, and expression analysis of superoxide dismutase (SOD) genes in foxtail millet (Setaria italica L.)

3 Biotech ◽  
2018 ◽  
Vol 8 (12) ◽  
Author(s):  
Tao Wang ◽  
Hui Song ◽  
Baohong Zhang ◽  
Quanwei Lu ◽  
Zhen Liu ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Expression Analysis ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Genome Wide

scholarly journals Genome-wide identification and in silico characterization of chitinase gene family in Foxtail millet (Setaria italica)

2020 ◽  
Author(s):  
Motukuri S. R. Krishna ◽  
Nerella Dheeraj ◽  
Bathuru Jayasree ◽  
Chodisetty Bhavya ◽  
Nallamothu Jaswanthi
Keyword(s):  
Gene Family ◽  
In Silico ◽  
Foxtail Millet ◽  
Chitinase Gene ◽  
Setaria Italica ◽  
Genome Wide ◽  
In Silico Characterization

Genome-wide identification and expression analysis of calmodulin-binding transcription activator genes in banana under drought stress

2019 ◽  
Vol 244 ◽  
pp. 10-14 ◽  
Author(s):  
Laraib Meer ◽  
Sana Mumtaz ◽  
Abdullahi Muhammad Labbo ◽  
Muhammad Jawad Khan ◽  
Irfan Sadiq
Keyword(s):  
Drought Stress ◽  
Expression Analysis ◽  
Transcription Activator ◽  
Calmodulin Binding ◽  
Genome Wide

scholarly journals Genome-Wide Gene Expression Profiles Analysis Reveal Novel Insights into Drought Stress in Foxtail Millet (Setaria italica L.)

2020 ◽  
Vol 21 (22) ◽  
pp. 8520
Author(s):  
Ling Qin ◽  
Erying Chen ◽  
Feifei Li ◽  
Xiao Yu ◽  
Zhenyu Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signal Transduction ◽  
Drought Stress ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Foxtail Millet ◽  
Gene Expression Profiles ◽  
Sugar Metabolism ◽  
Setaria Italica ◽  
Phenylpropanoid Biosynthesis

Foxtail millet (Setaria italica (L.) P. Beauv) is an important food and forage crop because of its health benefits and adaptation to drought stress; however, reports of transcriptomic analysis of genes responding to re-watering after drought stress in foxtail millet are rare. The present study evaluated physiological parameters, such as proline content, p5cs enzyme activity, anti-oxidation enzyme activities, and investigated gene expression patterns using RNA sequencing of the drought-tolerant foxtail millet variety (Jigu 16) treated with drought stress and rehydration. The results indicated that drought stress-responsive genes were related to many multiple metabolic processes, such as photosynthesis, signal transduction, phenylpropanoid biosynthesis, starch and sucrose metabolism, and osmotic adjustment. Furthermore, the Δ1-pyrroline-5-carboxylate synthetase genes, SiP5CS1 and SiP5CS2, were remarkably upregulated in foxtail millet under drought stress conditions. Foxtail millet can also recover well on rehydration after drought stress through gene regulation. Our data demonstrate that recovery on rehydration primarily involves proline metabolism, sugar metabolism, hormone signal transduction, water transport, and detoxification, plus reversal of the expression direction of most drought-responsive genes. Our results provided a detailed description of the comparative transcriptome response of foxtail millet variety Jigu 16 under drought and rehydration environments. Furthermore, we identify SiP5CS2 as an important gene likely involved in the drought tolerance of foxtail millet.

scholarly journals Genome-wide characterization and expression analysis of citrus NUCLEAR FACTOR-Y (NF-Y) transcription factors identified a novel NF-YA gene involved in drought-stress response and tolerance

PLoS ONE ◽  
2018 ◽  
Vol 13 (6) ◽  
pp. e0199187 ◽  
Author(s):  
Suzam L. S. Pereira ◽  
Cristina P. S. Martins ◽  
Aurizangela O. Sousa ◽  
Luciana R. Camillo ◽  
Caroline P. Araújo ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Drought Stress ◽  
Stress Response ◽  
Expression Analysis ◽  
Nuclear Factor ◽  
Nuclear Factor Y ◽  
Genome Wide

Foxtail millet WRKY genes and drought stress

2016 ◽  
Vol 155 (5) ◽  
pp. 777-790 ◽  
Author(s):  
L. ZHANG ◽  
H. SHU ◽  
A. Y. ZHANG ◽  
B. L. LIU ◽  
G. F. XING ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Quantitative Polymerase Chain Reaction ◽  
Foxtail Millet ◽  
Wrky Transcription Factor ◽  
Sequencing Data ◽  
Genome Wide ◽  
Functional Analyses ◽  
Encoding Genes

SUMMARYFoxtail millet (Setaria italica (L.) P. Beauv.) is a naturally stress-tolerant plant, a major reserve crop and a model for panicoid grasses. The recent completion of the S. italica genome facilitates identification and characterization of WRKY transcription factor family proteins that are important regulators of major plant processes, including growth, development and stress response. The present study identified 103 WRKY transcription factor-encoding genes in the S. italica genome. The genes were named SiWRKY1–SiWRKY103 according to their order on the chromosomes. A comprehensive expression analysis of SiWRKY genes among four different tissues was performed using publicly available RNA sequencing data. Eighty-four SiWRKY genes were more highly expressed in root tissue than in other tissues and nine genes were only expressed in roots. Additionally, real-time quantitative polymerase chain reaction was performed to comprehensively analyse the expression of all SiWRKY genes in response to dehydration. Results indicated that most SiWRKY genes (over 0.8) were up-regulated by drought stress. In conclusion, genome-wide identification and expression profiling of SiWRKY genes provided a set of candidates for cloning and functional analyses in plants’ response to drought stress.

scholarly journals Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.)

2018 ◽  
Author(s):  
Weiping Shi ◽  
Jingye Cheng ◽  
Xiaojie Wen ◽  
Jixiang Wang ◽  
Guanyan Shi ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Stress Responses ◽  
Molecular Mechanisms ◽  
Abiotic Factors ◽  
Chlorophyll Biosynthesis ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Parental Lines

Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular mechanisms responsible for drought stress responses is vital to improvement of agricultural production. In this study, we exposed the drought resistant F1 hybrid, M79, and its parental lines E1 and H1 to drought stress. Subsequent physiological analysis demonstrated that M79 showed higher photosynthetic energy conversion efficiency and drought tolerance than its parents. A transcriptomic study using leaves collected six days after drought treatment, when the soil water content was about ~20%, identified 3066, 1895, and 2148 differentially expressed genes (DEGs) in M79, E1 and H1 compared to the respective untreated controls, respectively. Further analysis revealed 17 Gene Ontology (GO) enrichments and 14 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in M79, including photosystem II (PSII) oxygen-evolving complex, peroxidase (POD) activity, plant hormone signal transduction, and chlorophyll biosynthesis. Co-regulation analysis suggested that these DEGs in M79 contributed to the formation of a regulatory network involving multiple biological processes and pathways including photosynthesis, signal transduction, transcriptional regulation, redox regulation, hormonal signaling, and osmotic regulation. RNA-seq analysis also showed that some photosynthesis-related DEGs were highly expressed in M79 compared to its parental lines under drought stress. These results indicate that various molecular pathways, including photosynthesis, respond to drought stress in M79, and provide abundant molecular information for further analysis of the underlying mechanism responding to this stress.

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