scholarly journals Direct visualization and quantification of bone growth into porous titanium implants using micro computed tomography

2011 ◽  
Vol 22 (5) ◽  
pp. 1321-1332 ◽  
Author(s):  
E. Baril ◽  
L. P. Lefebvre ◽  
S. A. Hacking
Keyword(s):  
Computed Tomography ◽  
Bone Growth ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Direct Visualization ◽  
Micro Computed Tomography

Micro-Computed Tomography Analysis on Administration of Mesenchymal Stem Cells - Bovine Teeth Scaffold Composites for Alveolar Bone Tissue Engineering

2021 ◽  
Vol 52 ◽  
pp. 86-96
Author(s):  
Desi Sandra Sari ◽  
Fourier Dzar Eljabbar Latief ◽  
Ferdiansyah ◽  
Ketut Sudiana ◽  
Fedik Abdul Rantam
Keyword(s):  
Computed Tomography ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Bone Growth ◽  
Alveolar Bone ◽  
Micro Computed Tomography ◽  
Freeze Dried ◽  
Significant Difference

The tissue engineering approach for periodontal tissue regeneration using a combination of stem cells and scaffold has been vastly developed. Mesenchymal Stem Cells (MSCs) seeded with Bovine Teeth Scaffold (BTSc) can repair alveolar bone damage in periodontitis cases. The alveolar bone regeneration process was analyzed by micro-computed tomography (µ-CT) to observe the structure of bone growth and to visualize the scaffold in 3-Dimensional (3D). The purpose of this study is to analyze alveolar bone regeneration by µ-CT following the combination of MSCs and bovine teeth scaffold (MSCs-BTSc) implantation in the Wistar rat periodontitis model. Methods. MSCs were cultured from adipose-derived mesenchymal stem cells of rats. BTSc was taken from bovine teeth and freeze-dried with a particle size of 150-355 µm. MSCs were seeded on BTSc for 24 hours and transplanted in a rat model of periodontitis. Thirty-five Wistar rats were made as periodontitis models with LPS induction from P. gingivalis injected to the buccal section of interproximal gingiva between the first and the second mandibular right-molar teeth for six weeks. There were seven groups (control group, BTSc group on day 7, BTSc group on day 14, BTSc group on day 28, MSCs-BTSc group on day 7, MSCs-BTSc group on day 14, MSCs-BTSc group on day 28). The mandibular alveolar bone was analyzed and visualized in 3D with µ-CT to observe any new bone growth. Statistical Analysis. Group data were subjected to the Kruskal Wallis test followed by the Mann-Whitney (p <0.05). The µ-CT qualitative analysis shows a fibrous structure, which indicates the existence of new bone regeneration. Quantitative analysis of the periodontitis model showed a significant difference between the control model and the model with the alveolar bone resorption (p <0.05). The bone volume and density measurements revealed that the MSCs-BTSc group on day 28 formed new bone compared to other groups (p <0.05). Administration of MSCs-BTSc combination has the potential to form new alveolar bone.

Bone Growth in Porous Titanium Implants Made by Rapid Prototyping

2006 ◽  
pp. 1099-1104
Author(s):  
Marco A. Lopez-Heredia ◽  
Eric Goyenvalle ◽  
Eric Aguado ◽  
C. Leroux ◽  
M. Dorget ◽  
...  
Keyword(s):  
Rapid Prototyping ◽  
Bone Growth ◽  
Porous Titanium ◽  
Titanium Implants

scholarly journals Parathyroid hormone enhances gap healing and osseointegration in orthopedic porous coated titanium implants: a correlative micro-computed tomographic, histomorphometric and biomechanical analysis

2022 ◽  
Vol 23 (1) ◽  
Author(s):  
Xinlin Gao ◽  
Yang Meng ◽  
Dingjun Hao ◽  
Hao Liu
Keyword(s):  
Computed Tomography ◽  
Parathyroid Hormone ◽  
Bone Formation ◽  
Low Dose ◽  
Titanium Implants ◽  
Biomechanical Analysis ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Pull Out ◽  
Custom Made

Abstract Background Parathyroid hormone, with its anabolic effect on bone formation, has shown excellent outcomes of curing postmenopausal osteoporosis as well as enhancing osseointegration around orthopaedic and stomatologic implants.The purpose of the present study is to test if low-dose intermittent PTH (1–34) treatment could achieve a satisfactory osseointegration in 2-mm peri-implant gaps, as to provide a new idea for improving the stability of such prosthesis, which will be of great clinical value. Methods A custom-made titanium implant was implanted on the calvarium of New Zealand White rabbits. 48 male rabbits were randomly divided into control and PTH group. PTH group received subcutaneous injection of PTH (20 μg/day, 5 days/week). Animals were sacrificed at 4 and 8 weeks after surgery. Quantitative micro-computed tomography, histology and biomechanical pull-out testing were performed to evaluate the gap healing at implantation site. Results Analysis of micro-computed tomography demonstrated that PTH group achieved more new bone formation in 2-mm gaps and on bone-implant interface. Quantitatively, significant differences were observed between two groups in regard to BIC and BV/TV at each time-point. Histological staining revealed that PTH group had a superiority in trabecular number, thickness, separation and better osseointegration compared to control group. As for biomechanical pull-out testing, PTH group also showed significant improvement of ultimate force than control group. Conclusions Low-dose intermittent administration of PTH for 4 and 8 weeks enhances early osseointegration and fixation of orthopedic implants surrounded by a 2-mm gap in terms of increased bone regeneration and mechanical stability. These findings suggest PTH a potential for reducing the postoperative complications of implants by improving bone healing at peri-implant gaps.

3-D Analysis of Pore Structure of Porous Biomaterials Using Micro Focus X-Ray Computed Tomography

2006 ◽  
Vol 309-311 ◽  
pp. 1095-1098 ◽  
Author(s):  
Mitsuru Takemoto ◽  
Shunsuke Fujibayashi ◽  
B. Otsuki ◽  
Tomiharu Matsushita ◽  
Tadashi Kokubo ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Image Processing ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Pore Throat ◽  
X Ray ◽  
X Ray Computed ◽  
Processing Software ◽  
Image Processing Software ◽  
Porous Biomaterials

Generally, characterizations of pore structures of porous biomaterials are mainly based on 2-dimensional (2-D) analysis using cross sectional micrographs. However, interconnectivity of each pore may be more important factor, when tissue ingrowth into deeper pores is considered. In this paper, using micro-CT imaging with 3-D image processing software, analyses of porous material based on 3-demensional (3-D) geometrical considerations were successfully performed. Plasmasprayed porous titanium implant (PT) and four types of sintered porous titanium implants (ST50- 200, ST50-500, ST70-200, and ST70-500) that possess different porosities (50% and 70%) and pore sizes (200-500+m and 500-1500+m) were analyzed in this study. A micro focus X-ray computed tomography system was employed to acquire microstructural information from the porous implants. Using 3-D image processing software, we performed three types of 3-D analysis including detection of the dead space (% dead pore), analysis of interconnectivity by blocking the narrow pore throat with caliber less than 52 +m (% pore with narrow throat) and analysis of material construct by contracting thin strut with thickness less than 52 +m (% construct with thin strut). ST50S and ST50L possessed interconnected porous structure with thicker strut; however, pore throat was considered to be relatively narrow. On the other hand, PT implant possesses favorable interconnectivity despite its’ low porosity; however, relatively thin strut indicate the structural disadvantage for mechanical property. These results suggest that the 3-D analysis of pore and strut structure using micro focus X-ray computed tomography and 3-D image processing software will provide effective information to develop porous implant.

Osteogenesis around CaP-coated titanium implants visualized using 3D histology and micro-computed tomography

2015 ◽  
Vol 103 (11) ◽  
pp. 3463-3473 ◽  
Author(s):  
Vincent M. J. I. Cuijpers ◽  
Hamdan S. Alghamdi ◽  
Natasja W. M. Van Dijk ◽  
Jakub Jaroszewicz ◽  
X. Frank Walboomers ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Titanium Implants ◽  
Micro Computed Tomography

scholarly journals Evaluation of Peri-Implant Bone Grafting Around Surface-Porous Dental Implants: An In Vivo Study in a Goat Model

Materials ◽  
2019 ◽  
Vol 12 (21) ◽  
pp. 3606 ◽  
Author(s):  
Fahad Alshehri ◽  
Mohammed Alshehri ◽  
Terrence Sumague ◽  
Abdurahman Niazy ◽  
John Jansen ◽  
...  
Keyword(s):  
Dental Implants ◽  
Bone Grafting ◽  
Bone Growth ◽  
Bone Ingrowth ◽  
Titanium Implant ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Biomechanical Assessment ◽  
Growth Area

Dental implants with surface-porous designs have been recently developed. Clinically, peri-implant bone grafting is expected to promote early osseointegration and bone ingrowth when applied with surface-porous dental implants in challenging conditions. The aim of this study was to comparatively analyze peri-implant bone healing around solid implants and surface-porous implants with and without peri-implant bone grafting, using biomechanical and histomorphometrical assessment in a goat iliac bone model. A total of 36 implants (4.1 mm wide, 11.5 mm long) divided into three groups, solid titanium implant (STI; n = 12), porous titanium implants (PTI; n = 12) and PTI with peri-implant bone grafting using biphasic calcium phosphate granules (PTI + BCP; n = 12), were placed bilaterally in the iliac crests of six goats. The goats were sacrificed seven weeks post-operatively and then subjected to biomechanical (n = 6 per group) and histomorphometrical (n = 6 per group) assessment. The biomechanical assessment revealed no significant differences between the three types of implants. Although the peri-implant bone-area (PIBA%) measured by histomorphometry (STI: 8.63 ± 3.93%, PTI: 9.89 ± 3.69%, PTI + BCP: 9.28 ± 2.61%) was similar for the three experimental groups, the percentage of new bone growth area (BGA%) inside the porous implant portion was significantly higher (p < 0.05) in the PTI group (10.67 ± 4.61%) compared to the PTI + BCP group (6.50 ± 6.53%). These data demonstrate that peri-implant bone grafting around surface-porous dental implants does not significantly accelerate early osseointegration and bone ingrowth.

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