Effects of postharvest storage and dormancy status on ABA content, metabolism, and expression of genes involved in ABA biosynthesis and metabolism in potato tuber tissues

2006 ◽  
Vol 61 (4-5) ◽  
pp. 687-697 ◽  
Author(s):  
Luis Destefano-Beltrán ◽  
Donna Knauber ◽  
Linda Huckle ◽  
Jeffrey C. Suttle
Keyword(s):  
Potato Tuber ◽  
Postharvest Storage ◽  
Expression Of Genes ◽  
Aba Content ◽  
Aba Biosynthesis

scholarly journals Influence of γ-irradiation on the expression of encoding ABA metabolism enzymes in barley embryos

2018 ◽  
Vol 16 (4) ◽  
pp. 85-89
Author(s):  
Sofia V. Bitarishvili ◽  
Vladimir S. Bondarenko ◽  
Stanislav A. Geras’kin
Keyword(s):  
Dose Range ◽  
Γ Irradiation ◽  
Aba Metabolism ◽  
Expression Of Genes ◽  
Metabolism Enzymes ◽  
Small Doses ◽  
Genes Encoding ◽  
Aba Content ◽  
Aba Biosynthesis ◽  
Relative Gene

Background. Small doses of radiation stimulate the growth and development of plants including seed germination. ABA plays a key role not only in seed dormancy and germination but also in the regulation of adaptive reactions of plants. The aim of our work was to study the effect of γ-irradiation of barley seeds in a small doses on the expression of genes encoding ABA biosynthesis enzyme 9-cis-epoxycarotenoid dioxygenase (HvNCED1) and catabolism enzyme ABA 8’-hydroxylase (HvABA8’OH-1). Materials and Methods. The barley seeds were irradiated at dose range of 4–50 Gy at a dose rate of 60 Gy/h, the radiation source was 60Co. The study was carried out in the embryos within the first 30 hours after germination. Relative gene expression was investigated using real-time PCR (RT-PCR). Results. It was shown that γ-irradiation of barley seeds changes the expression of ABA biosynthesis and catabolism genes in all experimental groups. Conclusion. This alterations can lead to a decrease the ABA content under irradiation with stimulating doses and increase the biosynthesis of phytohormone under irradiation with inhibitory dose.

scholarly journals Integrative hormone and transcriptome analyses underline the role of abscisic acid in seed shattering of weedy rice

2020 ◽  
Author(s):  
Hong Lang ◽  
Yuting He ◽  
Jian Sun ◽  
Fengcheng Li ◽  
Dianrong Ma
Keyword(s):  
Abscisic Acid ◽  
Rice Variety ◽  
Critical Role ◽  
Weedy Rice ◽  
Seed Shattering ◽  
Expression Of Genes ◽  
Aba Content ◽  
Potential Signal ◽  
Aba Biosynthesis

Abstract Abstract Backgrounds: Weedy rice is one of the most severe weeds in paddy fields, strongly characterized by its high seed shattering level. Abscisic acid (ABA) serves as an abscission-accelerating signal and plays a critical role during abscission. However, mechanisms that link ABA and seed shattering remain elusive. In this study, we compared WR04-6 a shattering, and SN9816 non-shattering rice variety for genetic expression and ABA levels in the abscission zone (AZ) and the spikelet. Results : ABA content in WR04-6, particularly in AZ, was significantly higher than that in SN9816, and it increased remarkably prior to abscission. Transcriptomic analysis and qRT-PCR showed that the expression of NCED , the key gene in ABA biosynthesis, coincided with increased ABA content in AZ and increased significantly during the seed shattering process. Additionally, the expression of genes related biosynthesis and metabolism of IAA, GA, and ETH showed the greatest fold change. Phytohormone levels associated with ABA co-expression-prediction revealed a potential signal transduction network among plant hormones involved in regulating seed abscission. Conclusions: Altogether, our data strongly indicated that ABA contributes to seed shattering and appears to transiently cooperate with other hormones, triggering a hormone imbalance that leads to the downstream activation of AZ

Vegetation-derived abscisic acid and four terpenes enforce dormancy in seeds of the post-fire annual,Nicotiana attenuata

2002 ◽  
Vol 12 (4) ◽  
pp. 239-252 ◽  
Author(s):  
Bernd Krock ◽  
Sybille Schmidt ◽  
Christian Hertweck ◽  
Ian T. Baldwin
Keyword(s):  
Abscisic Acid ◽  
Environmental Control ◽  
Wood Smoke ◽  
Nicotiana Attenuata ◽  
Germination Inhibitors ◽  
Physiological Basis ◽  
Fire Environment ◽  
Aba Content ◽  
Aba Biosynthesis ◽  
Endogenous Aba

AbstractThe native tobacco,Nicotiana attenuata, synchronizes its germination with the immediate post-fire environment with a combination of germination stimulants found in wood smoke and inhibitors from the unburned litter of the dominant vegetation. The inhibitors override the stimulants and prevent seeds from germinating maladaptively in unburned habitats adjacent to burns. To understand the physiological basis of this environmental control of germination, we tested several previously isolated signals, phytohormones and their respective biosynthesis inhibitors. The germination inhibitors methyl jasmonate (MeJA, a constituent of sagebrush litter), bornane-2,5-dione (BD, a constituent of juniper litter extract, JLE) and JLE did not alter abscisic acid (ABA) content of imbibed seeds. Treatment with the ABA biosynthesis inhibitor, fluridone, inhibited the dormancy-inducing effects of BD, JLE and MeJA, but surprisingly did not affect endogenous ABA levels in treated seeds. However, ABA leached from litter of the species, which dominate the plant community before fires, plays an important role in germination control. We conclude thatN. attenuataseeds, which can lie dormant in the soil for 150 years between fires, time their germination with the post-fire environment by responding to smoke, ABA and four terpenes (BD, 1,8-cineole, β-thujaplicin and camphor) leaching from the litter of the dominant vegetation.

scholarly journals Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in Grape Berry of Vitis vinifera cv. Kyoho

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 12 ◽  
Author(s):  
Mingyi Yang ◽  
Lei Wang ◽  
Tarun Belwal ◽  
Xiaocheng Zhang ◽  
Hongyan Lu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Vitis Vinifera ◽  
Agricultural Production ◽  
High Performance ◽  
Nordihydroguaiaretic Acid ◽  
Underlying Mechanism ◽  
Grape Berry ◽  
Flight Mass Spectrometry ◽  
Expression Of Genes ◽  
Aba Biosynthesis

Grape polyphenols contributing to more than half of the global polyphenol market were well studied; however, how melatonin (MLT), a potential plant hormone, and abscisic acid (ABA) affects polyphenols profile is still poorly understood. To explore whether these hormones are involved in polyphenolic biosynthesis, grape (Vitis vinifera cv. Kyoho) was exposed to MLT, ABA, and NDGA (nordihydroguaiaretic acid, an ABA biosynthesis inhibitor) treatments, and 16 polyphenols were identified from grape extracts by high performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS). Both exogenous MLT and ABA significantly enhanced the biosynthesis of each flavonol and flavanol component, especially catechin, which was almost increased double by 200 µM of MLT treatment. Furthermore, the expression of genes involved in flavonoid biosynthesis, including 4-coumaroyl-CoA synthase, chalcone synthase, flavonoid 3′-hydroxylase, anthocyanin 3′-methyltransferase, flavonol synthase, flavonoid-3-O-glucosyltransferase, and flavonoid 3′,5′-methyltransferase were highly up-regulated as well but were down-regulated by NDGA. The present study provided new insights for improving flavonoids accumulation in agricultural production and its underlying mechanism.

scholarly journals Transcriptomic Analysis Reveals the Positive Role of Abscisic Acid in Endodormancy Maintenance of Leaf Buds of Magnolia wufengensis

2021 ◽  
Vol 12 ◽  
Author(s):  
Kunjing Wu ◽  
Xiaojing Duan ◽  
Zhonglong Zhu ◽  
Ziyang Sang ◽  
Yutong Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Rna Sequencing ◽  
Average Length ◽  
Transcriptomic Analysis ◽  
Bud Dormancy ◽  
Positive Role ◽  
Cold Environments ◽  
Expression Of Genes ◽  
Aba Content ◽  
Magnolia Wufengensis

Magnolia wufengensis (Magnoliaceae) is a deciduous landscape species, known for its ornamental value with uniquely shaped and coloured tepals. The species has been introduced to many cities in south China, but low temperatures limit the expansion of this species in cold regions. Bud dormancy is critical for plants to survive in cold environments during the winter. In this study, we performed transcriptomic analysis of leaf buds using RNA sequencing and compared their gene expression during endodormancy, endodormancy release, and ecodormancy. A total of 187,406 unigenes were generated with an average length of 621.82 bp (N50 = 895 bp). In the transcriptomic analysis, differentially expressed genes involved in metabolism and signal transduction of hormones especially abscisic acid (ABA) were substantially annotated during dormancy transition. Our results showed that ABA at a concentration of 100 μM promoted dormancy maintenance in buds of M. wufengensis. Furthermore, the expression of genes related to ABA biosynthesis, catabolism, and signalling pathway was analysed by qPCR. We found that the expression of MwCYP707A-1-2 was consistent with ABA content and the dormancy transition phase, indicating that MwCYP707A-1-2 played a role in endodormancy release. In addition, the upregulation of MwCBF1 during dormancy release highlighted the enhancement of cold resistance. This study provides new insights into the cold tolerance of M. wufengensis in the winter from bud dormancy based on RNA-sequencing and offers fundamental data for further research on breeding improvement of M. wufengensis.

scholarly journals OsWRKY5 Promotes Rice Leaf Senescence via Senescence-Associated NAC and Abscisic Acid Biosynthesis Pathway

2019 ◽  
Vol 20 (18) ◽  
pp. 4437 ◽  
Author(s):  
Kim ◽  
Kang ◽  
Kim ◽  
An ◽  
Paek
Keyword(s):  
Abscisic Acid ◽  
Leaf Senescence ◽  
Chlorophyll Degradation ◽  
Regulatory Function ◽  
Abscisic Acid Biosynthesis ◽  
Positive Regulator ◽  
Expression Of Genes ◽  
Dna Insertion ◽  
Aba Biosynthesis ◽  
Endogenous Aba

he onset of leaf senescence is triggered by external cues and internal factors such as phytohormones and signaling pathways involving transcription factors (TFs). Abscisic acid (ABA) strongly induces senescence and endogenous ABA levels are finely tuned by many senescence-associated TFs. Here, we report on the regulatory function of the senescence-induced TF OsWRKY5 TF in rice (Oryza sativa). OsWRKY5 expression was rapidly upregulated in senescing leaves, especially in yellowing sectors initiated by aging or dark treatment. A T-DNA insertion activation-tagged OsWRKY5-overexpressing mutant (termed oswrky5-D) promoted leaf senescence under natural and dark-induced senescence (DIS) conditions. By contrast, a T-DNA insertion oswrky5-knockdown mutant (termed oswrky5) retained leaf greenness during DIS. Reverse-transcription quantitative PCR (RT-qPCR) showed that OsWRKY5 upregulates the expression of genes controlling chlorophyll degradation and leaf senescence. Furthermore, RT-qPCR and yeast one-hybrid analysis demonstrated that OsWRKY5 indirectly upregulates the expression of senescence-associated NAM/ATAF1/2/CUC2 (NAC) genes including OsNAP and OsNAC2. Precocious leaf yellowing in the oswrky5-D mutant might be caused by elevated endogenous ABA concentrations resulting from upregulated expression of ABA biosynthesis genes OsNCED3, OsNCED4, and OsNCED5, indicating that OsWRKY is a positive regulator of ABA biosynthesis during leaf senescence. Furthermore, OsWRKY5 expression was suppressed by ABA treatment. Taken together, OsWRKY5 is a positive regulator of leaf senescence that upregulates senescence-induced NAC, ABA biosynthesis, and chlorophyll degradation genes.

Integrative hormone and transcriptome analysis underline the role of abscisic acid in seed shattering of weedy rice

2021 ◽  
Author(s):  
Hong Lang ◽  
Yuting He ◽  
Fengcheng Li ◽  
Dianrong Ma ◽  
Jian Sun
Keyword(s):  
Abscisic Acid ◽  
Critical Role ◽  
Weedy Rice ◽  
Seed Shattering ◽  
High Degree ◽  
Aba Content ◽  
Potential Signal ◽  
Aba Biosynthesis ◽  
Indole 3 Acetic Acid

AbstractWeedy rice is one of the most severe weeds in paddy fields, characterized by its high degree of seed shattering. Abscisic acid (ABA) serves as an abscission-accelerating signal and plays a critical role during abscission. However, mechanisms that link ABA and seed shattering remain elusive. In this study, WR04-6 (shattering) and SN9816 (non-shattering) were used to investigate the expression levels of genes involved in ABA biosynthesis and to determine the levels of ABA in tissues collected from the abscission zone (AZ) and the spikelet. ABA content in WR04-6, particularly in AZ, was significantly higher than in SN9816, significantly increasing prior to abscission. RNA-Sequencing and further expression analyses showed that the expression of OsNCED, the key gene involved in ABA biosynthesis, coincided with the increase of ABA content in the AZ and significantly increased during the seed shattering process. Additionally, the expression analysis of genes related to biosynthesis and metabolism of indole-3-acetic acid, gibberellin acid, and ethylene showed the greatest fold-change. Phytohormone levels associated with ABA co-expression-prediction revealed a potential signal transduction network among plant hormones involved in the regulation of seed abscission. Taken together, data presented in this study suggest that ABA contributes to seed shattering and transiently cooperates with other hormones, triggering a hormone imbalance that leads to the downstream activation of the AZ.

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